Dermatophytes’ identification by Matrix-assisted laser desorption ionization-time of flight mass spectrometry. (MALDI-TOF MS) - the experience of a clinical laboratory

Objectives: Dermatophytes are a challenging group of fungi that infect the keratinized tissues. The taxonomy of these fungi has changed recently with the reclassification of some species and description of new ones. However, many clinical laboratories still base the identification of dermatophytes on their phenotype. Since dermatophytes are very pleomorphic, macro and micromorphology are often insufficient to reach a correct classification and may lead to misidentifications. The identification based on MALDI-TOF relies on the protein profile of the microorganism. Thus, this study aims to summarize our current laboratorial experience of dermatophyte identification using MALDI-TOF MS. Methods: From january to april 2018, 95 dermatophytes isolates, collected from human keratinized samples and also from quality control programs were characterized by phenotypic analysis, and by VITEK MS V3.2 bioMerieux. Before identification procedure, isolates were inoculated on Sabouraud Dextrose agar plates and incubated at 27°C during 5 to 10 days. Species were identified taking into account clinical features, as well as cultural, microscopic and physiological characteristics. Prior to MALDI-TOF MS analysis, the samples were pre-treated according to the manufacturer’s protocol for filamentous fungi. Molecular identification by sequencing of the internal transcribed spacer 1 (ITS1) was performed in 34 of those isolates Results: Through phenotypic analysis eight different species were identified (54 Trichophyton rubrum; 4 T.soudanense; 22 T.interdigitale; 1 T.mentagrophytes; 3 T.tonsurans; 7 Microsporum canis; 3 M.audouinii; 1 Microsporum spp.- (non canis or audouinii). MALDI-TOF analysis showed an identification agreement in 80 cases (84,2%) with a confidence level of 99,9%. Eight isolates showed divergent identification results: three T.rubrum were identified as T.violaceum, three T.soudanense were identified as T.rubrum, one T.mentagrophytes was identified as T.interdigitale and one T.tonsurans was identified as T.rubrum. In four cases MALDI-TOF analysis did not get a profile. The ITS sequencing analysis of discrepant results corroborated the MALDI-TOF identification in five of them. On the other hand, T.soudanense was only identified by phenotypic analysis since MALDI-TOF and ITS sequencing result was T.rubrum. MALDITOF identification of T.violaceum was not confirmed by ITS sequencing that identified T. rubrum instead, in accordance with the phenotypic identification. Conclusion: Correct identification of dermatophytes to species level requires sequencing of the ITS, LSU, and/or betatubulin regions. The implementation of this methodology in a clinical laboratory is expensive and time consuming. MALDI-TOF identification is a good option for dermatophytes’ identification performed in laboratory routine, since costs of consumables as well as time of sample preparation are lower than for PCR analysis and doesn’t require long training period as phenotypic identification does. In this study, however, both methods failed to identify some species variants like Trichophyton soudanense or T. violaceum. The combined use of both MALDI-TOF and phenotypic methods seems to be the better approach for dermatophytes’ identification since some species show significant phenotypic and clinical differences.info:eu-repo/semantics/publishedVersio

O mercado de luxo e o marketing holístico: o caso dos hotéis de luxo

No atual contexto económico de crise, o mercado de luxo conseguiu cresceu, porque muitos dos clientes de luxo mantêm o poder financeiro e conservam a sua ambição por luxo. No entanto, num mercado caracterizado por intensa concorrência não é suficiente ter uma marca forte, é imprescindível acrescentar valor e o marketing holístico pode contribuir fortemente para esse resultado. Nesse sentido, o objetivo deste artigo é analisar as especificidades do mercado de luxo comparativamente com o mercado de bens comuns, e avaliar o nível de valorização e aplicação pelas marcas de luxo de cada uma das quatro dimensões do marketing holístico (marketing relacional, marketing interno, marketing performance e marketing integrado). Para o efeito foi escolhido o setor de turismo de luxo português, mais concretamente, os hotéis de luxo. Os dados foram recolhidos por via da realização de entrevistas aos diretores hoteleiros ou gestores de marketing de seis hotéis de luxo localizados em Portugal e foram tratados seguindo uma metodologia qualitativa de análise temática de conteúdo por recurso ao software MAXqda®. Os resultados denotam que o mercado de luxo é mais complexo de gerir do que o mercado de bens comuns e realçam que os hotéis de luxo privilegiam cada uma das dimensões inerentes à filosofia de marketing holístico. Este artigo faculta informações importantes que podem contribuir para as recentes teorias e práticas de marketing no mercado de luxo

Language identification: a neural network approach

Series : OASIcs - Open access series in informatics, ISSN 2190-6807, vol. 38One of the first tasks when building a Natural Language application is the detection of the used language in order to adapt the system to that language. This task has been addressed several times. Nevertheless most of these attempts were performed a long time ago when the amount of computer data and the computational power were limited. In this article we analyze and explain the use of a neural network for language identification, where features can be extracted automatically, and therefore, easy to adapt to new languages. In our experiments we got some surprises, namely with the two Chinese variants, whose forced us for some language-dependent tweaking of the neural network. At the end, the network had a precision of 95%, only failing for the Portuguese language.(undefined

Indicadores para a avaliação da disponibilidade hídrica para o desenvolvimento sustentável da atividade canavieira irrigada.

Para a racionalização do uso dos recursos hídricos e promoção do desenvolvimento sustentável da atividade canavieira na região Centro-Oeste torna-se importante o desenvolvimento de procedimentos metodológicos que possam fornecer subsídios técnicos para o planejamento e gestão setorial - agricultura e recursos hídricos. O presente trabalho apresenta dois indicadores concebidos para a avaliação do potencial da disponibilidade hídrica para atender a demanda projetada de água para o desenvolvimento da atividade canavieira

Scrutinising the exceptionalism of young rural NEETs: A bibliometric review

The situation of rural NEETs aged 15 to 24 remains understudied. However, transitions from adolescence to emerging adulthood are very demanding for those in the countryside. Our paper discusses this gap by characterising the scholarship focusing on rural NEETs. We undertook a bibliometric review based on 325 entries on Web of Science (WoS) using the Bibliometrix analysis package. Our approach included descriptive bibliometric analysis, co-citation networks assessment, and thematic analysis. Our findings show that the investigation efforts depicting younger rural NEET are recent and marginal in the larger context of international NEETs scholarship. The field is dominated by economy- and sociology-led networks. Concerns regarding health and employment issues are central in international publishing trends, showing a dominant youth-at-risk approach to this group. Still, themes associated with adolescent NEETs and relevant programs’ assessment are gaining traction. Our findings show a need for funding research initiatives to reduce the invisibility of young rural NEETs.info:eu-repo/semantics/acceptedVersio

Microscopy studies in support of dating of mortars of historical buildings

Fundação das Universidades PortuguesasMinisterio de Ciencia e InnovaciónFundação para a Ciência e a Tecnologia (FCT

Sistema de indicadores para a avaliação da sustentabilidade hídrica da atividade canaviera.

O objetivo deste trabalho é apresentar um estudo de caso realizado na Bacia Hidrográfica do Rio Verde (BHRV) para demonstrar a aplicabilidade do Sistema de Indicadores - SISHidro-Cana - para a avaliação da sustentabilidade hídrica da cultura canavieira, proposta por Ferraz (2012), como uma metodologia para avaliar a disponibilidade de água de bacias hidrográficas para subsidiar a proposição de políticas setoriais, ordenação territorial, planejamento agrícola e gestão dos recursos hídricos

Algumas considerações sobre o estudo estatístico de medições de dose em grãos de argamassas por luminescência estimulada opticamente

Fundação das Universidades PortuguesasMinisterio de Ciencia e Innovación (Espanha)Fundação para a Ciência e a Tecnologia (FCT)Ação Integrada E-141”(Fundação das Universidades Portuguesas)/“Acción Integrada PT2009-0077” (Ministerio de Ciencia e Innovación)/PEst-OE/CTE/UI0697/201