A fully-automated low-cost cardiac monolayer optical mapping robot.

Scalable and high-throughput electrophysiological measurement systems are necessary to accelerate the elucidation of cardiac diseases in drug development. Optical mapping is the primary method of simultaneously measuring several key electrophysiological parameters, such as action potentials, intracellular free calcium and conduction velocity, at high spatiotemporal resolution. This tool has been applied to isolated whole-hearts, whole-hearts in-vivo, tissue-slices and cardiac monolayers/tissue-constructs. Although optical mapping of all of these substrates have contributed to our understanding of ion-channels and fibrillation dynamics, cardiac monolayers/tissue-constructs are scalable macroscopic substrates that are particularly amenable to high-throughput interrogation. Here, we describe and validate a scalable and fully-automated monolayer optical mapping robot that requires no human intervention and with reasonable costs. As a proof-of-principle demonstration, we performed parallelized macroscopic optical mapping of calcium dynamics in the well-established neonatal-rat-ventricular-myocyte monolayer plated on standard 35 mm dishes. Given the advancements in regenerative and personalized medicine, we also performed parallelized macroscopic optical mapping of voltage dynamics in human pluripotent stem cell-derived cardiomyocyte monolayers using a genetically encoded voltage indictor and a commonly-used voltage sensitive dye to demonstrate the versatility of our system.The Centro Nacional de Investigaciones Cardiovasculares (CNIC) is supported by the MCIN and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (CEX2020-001041-S). The study was supported by the Ministry of Science and Innovation (MCIN) (PID2019-109329RB-I00), the Fundación Interhospitalaria para la Investigación Cardiovascular, the McEwen Stem Cell Institute, the Canada Research Chairs Program, the Stem Cell Network, the University of Toronto’s Medicine by Design/Canada First Research Excellence Fund initiative, and Ted Rogers Centre for Heart Research Education Fund.S

A fully-automated low-cost cardiac monolayer optical mapping robot

Scalable and high-throughput electrophysiological measurement systems are necessary to accelerate the elucidation of cardiac diseases in drug development. Optical mapping is the primary method of simultaneously measuring several key electrophysiological parameters, such as action potentials, intracellular free calcium and conduction velocity, at high spatiotemporal resolution. This tool has been applied to isolated whole-hearts, whole-hearts in-vivo, tissue-slices and cardiac monolayers/tissue-constructs. Although optical mapping of all of these substrates have contributed to our understanding of ion-channels and fibrillation dynamics, cardiac monolayers/tissue-constructs are scalable macroscopic substrates that are particularly amenable to high-throughput interrogation. Here, we describe and validate a scalable and fully-automated monolayer optical mapping robot that requires no human intervention and with reasonable costs. As a proof-of-principle demonstration, we performed parallelized macroscopic optical mapping of calcium dynamics in the well-established neonatal-rat-ventricular-myocyte monolayer plated on standard 35 mm dishes. Given the advancements in regenerative and personalized medicine, we also performed parallelized macroscopic optical mapping of voltage dynamics in human pluripotent stem cell-derived cardiomyocyte monolayers using a genetically encoded voltage indictor and a commonly-used voltage sensitive dye to demonstrate the versatility of our system

SERVEZ VOUS SANTÉ. Project d'intervention éducative sur les soins personnels et les habitudes saines chez les groupes vulnérables des Centres de soins de toxicomanie (CAD) des districts de Tetuán et A

Así mismo, se ha llevado a cabo el desarrollo de un TFG en metodología ApS, mediante la elaboración de una "Guía básica de cuidados para cuidadores y familiares de enfermos de Alzheimer" solicitada por la Asociación de Enfermos de Alzheimer de Coslada.El objetivo de este proyecto "Aprendizaje por Servicio" ha sido formar en autocuidados y hábitos saludables a colectivos vulnerables y en riesgo de exclusión social. Se ha trabajado con grupos de “personas sin hogar” (vinculado al centro de atención a las Adicciones del distrito de Arganzuela) y grupos de “jóvenes inmigrantes marroquíes”, muchos de ellos en situación de consumo crónico, que acuden a los Centros de Atención a las Adicciones (CAD) de los distritos de Tetuán y de Arganzuela del Ayuntamiento de Madrid. El proyecto se ha desarrollado dentro de una asignatura optativa de grado, de 3 ECTS, basada en metodología Aprendizaje por Servicio que tiene carácter transversal y es ofertada al alumnado de 3º y 4º curso de todas las titulaciones impartidas por la Facultad: Grado en Enfermería, Grado en Fisioterapia y Grado en Podología.The objective of this "Service Learning" project has been to train vulnerable groups at risk of social exclusion in self-care and healthy habits. Work has been done with groups of “homeless people” (linked to the Addictions Care Center of the Arganzuela district) and groups of “young Moroccan immigrants”, many of them in situations of chronic consumption, who go to the Care Centers. Addictions (CAD) of the Tetuán and Arganzuela districts of the Madrid City Council. The project has been developed within an optional undergraduate subject, worth 3 ECTS, based on Service Learning methodology that has a transversal nature and is offered to 3rd and 4th year students of all degrees taught by the Faculty: Degree in Nursing, Degree in Physiotherapy and Degree in Podiatry.L'objectif de ce projet « Service Learning » a été de former les groupes vulnérables menacés d'exclusion sociale aux soins personnels et aux habitudes saines. Un travail a été réalisé avec des groupes de « sans-abri » (liés au Centre de Soins des Addictions du quartier Arganzuela) et des groupes de « jeunes immigrés marocains », dont beaucoup sont en situation de consommation chronique, qui se rendent dans les Centres de Soins des Addictions ( CAD) des quartiers Tetuán et Arganzuela de la Mairie de Madrid. Le projet a été développé dans le cadre d'une matière facultative de premier cycle, d'une valeur de 3 ECTS, basée sur une méthodologie d'apprentissage par le service qui a un caractère transversal et est proposée aux étudiants de 3ème et 4ème années de tous les diplômes enseignés par la Faculté : diplôme en sciences infirmières, diplôme en physiothérapie et Diplôme en Podologie.Depto. de EnfermeríaDepto. de Psicología Experimental, Procesos Cognitivos y LogopediaSección Deptal. de Radiología, Rehabilitación y Fisioterapia (Enfermería)Fac. de Enfermería, Fisioterapia y PodologíaTRUEunpu

Siéntete un héroe. Salva vidas. Tercera edición

Proyecto orientado a formas a adolescentes en el manejo de situaciones de emergencia.Este proyecto es la continuación de dos proyectos de ApS aprobados en los cursos 2021-2022 y 2022-2023, y ha sido realizado con el Ayuntamiento de Coslada, estudiantes de la E.S.O., nuestros estudiantes UCM y miembros de la Facultad de Enfermería, Fisioterapia y Podología. Señalar la excelente valoración por todas las partes implicadas en esta 3ª edición, destacando como fortalezas la enseñanza entre iguales y la mejora en la capacidad de responder ante situaciones de emergencia de manera eficaz y segura para todos, en jóvenes de institutos. Ser capaz de salvar vidas es un aprendizaje de gran utilidad para toda la vida.Universidad Complutense de MadridDepto. de EnfermeríaFac. de Enfermería, Fisioterapia y PodologíaTRUEsubmittedAPC financiada por la UC

Non-invasive electromechanical assessment during atrial fibrillation identifies underlying atrial myopathy alterations with early prognostic value

Electromechanical characterization during atrial fibrillation (AF) remains a significant gap in the understanding of AF-related atrial myopathy. This study reports mechanistic insights into the electromechanical remodeling process associated with AF progression and further demonstrates its prognostic value in the clinic. In pigs, sequential electromechanical assessment during AF progression shows a progressive decrease in mechanical activity and early dissociation from its electrical counterpart. Atrial tissue samples from animals with AF reveal an abnormal increase in cardiomyocytes death and alterations in calcium handling proteins. High-throughput quantitative proteomics and immunoblotting analyses at different stages of AF progression identify downregulation of contractile proteins and progressive increase in atrial fibrosis. Moreover, advanced optical mapping techniques, applied to whole heart preparations during AF, demonstrate that AF-related remodeling decreases the frequency threshold for dissociation between transmembrane voltage signals and intracellular calcium transients compared to healthy controls. Single cell simulations of human atrial cardiomyocytes also confirm the experimental results. In patients, non-invasive assessment of the atrial electromechanical relationship further demonstrate that atrial electromechanical dissociation is an early prognostic indicator for acute and long-term rhythm control. Electromechanical characterization during atrial fibrillation (AF) remains a significant gap in the understanding of AF-related atrial myopathy. Here, the authors use non-invasive atrial electromechanical assessment during AF to identify early remodeling changes associated with underlying myopathy, which in the clinic decrease the probability of acute and mid-term successful rhythm control

Non-invasive electromechanical assessment during atrial fibrillation identifies underlying atrial myopathy alterations with early prognostic value.

Electromechanical characterization during atrial fibrillation (AF) remains a significant gap in the understanding of AF-related atrial myopathy. This study reports mechanistic insights into the electromechanical remodeling process associated with AF progression and further demonstrates its prognostic value in the clinic. In pigs, sequential electromechanical assessment during AF progression shows a progressive decrease in mechanical activity and early dissociation from its electrical counterpart. Atrial tissue samples from animals with AF reveal an abnormal increase in cardiomyocytes death and alterations in calcium handling proteins. High-throughput quantitative proteomics and immunoblotting analyses at different stages of AF progression identify downregulation of contractile proteins and progressive increase in atrial fibrosis. Moreover, advanced optical mapping techniques, applied to whole heart preparations during AF, demonstrate that AF-related remodeling decreases the frequency threshold for dissociation between transmembrane voltage signals and intracellular calcium transients compared to healthy controls. Single cell simulations of human atrial cardiomyocytes also confirm the experimental results. In patients, non-invasive assessment of the atrial electromechanical relationship further demonstrate that atrial electromechanical dissociation is an early prognostic indicator for acute and long-term rhythm control.This work was supported by the European Union Horizon 2020 research and innovation program under Grant Agreement#965286. The study was also supported by the Ministry of Science and Innovation (MCIN) (PID2019- 109329RB-I00 and PGC2018-097019-B-I00) funded by MCIN / AEI / 10.13039/501100011033, the Instituto de Salud Carlos III (Fondo de Investigación Sanitaria grant PRB3) (PT17/0019/0003- ISCIII-SGEFI / ERDF, ProteoRed), the Fundación Interhospitalaria para la Investigación Cardiovascular, the Fundación Salud 2000 and by “la Caixa” Banking Foundation (project code HR17-00247). The Centro Nacional de Investigaciones Cardiovasculares (CNIC) is supported by the Instituto de Salud Carlos III (ISCIII), the Ministerio de Ciencia e Innovación (MCIN) and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (CEX2020- 001041-S funded by MICIN/AEI/10.13039/501100011033). We also thank Asunción Conde and Sergey Mironov for their support on monitoring database quality and advice in ECG signal processing, respectively.S

SIRVE-T-SALUD. PROJET D'INTERVENTION ÉDUCATIVE SUR LES SOINS AUTO-SOINS ET LES HABITUDES SAINES DANS LES GROUPES VULNÉRABLES DES CENTRES DE SOINS ADDICTION (CAD) DES DISTRICTS DE TETUÁN ET ARGANZUELA (2e édition)

Nuestro estudiantado ha colaborado en la formación en autocuidados y hábitos saludables a colectivos vulnerables y/o en riesgo de exclusión social, mediante el desarrollo de talleres colaborativos y proactivos, diseñados y orientados a diferentes grupos de usuarios de los centros de atención a las adicciones (CAD) de los distritos de Tetuán y Arganzuela. En el CAD de Tetuán se trabajó con un grupo de “menores de 18 años en sanción administrativa” por consumo de cannabis que habían sido sancionados en vía pública y, con un “grupo de adultos con problemática específica de consumo Chemsex”. En el caso del CAD de Arganzuela se colaboró con un grupo de “adultos en prevención de recaídas, con abstinencia más o menos consolidada”. Las intervenciones del alumnado aportaron su pequeño grano de arena al Plan de Adicciones de la ciudad de Madrid 2022-2026, colaborando en alguno de los apartados de las líneas estratégicas que plantea: • “Prevención frente a los riesgos relacionados con las adicciones (sensibilizando, informando y formando a toda la población y, de forma específica, a los jóvenes,..” • “Atención integral a adolescentes y jóvenes, tanto los que presentan una situación de riesgo como quienes cumplen criterios de adicción...” • “Reducción de los riesgos y daños asociados a las adicciones..."Depto. de EnfermeríaSección Deptal. de Radiología, Rehabilitación y Fisioterapia (Enfermería)Depto. de Psicología Experimental, Procesos Cognitivos y LogopediaFac. de Enfermería, Fisioterapia y PodologíaTRUEsubmittedAPC financiada por la UC

Non-invasive electromechanical assessment during atrial fibrillation identifies underlying atrial myopathy alterations with early prognostic value

Depto. de MedicinaFac. de MedicinaFALSEpu