Genetic evaluation for productive traits in endangered Portuguese Malhado de Alcobaça pig

Malhado de Alcobaça (MA) is a swine breed from center west Portugal, recognized in 2003. Herdbook (LGMA) has information of >17,000 animals (1985-2022). It´s a very endangered breed with only 244 sows, 18 boars and 12 breeders. Within the scope of MA’s genetic conservation plan, for genetic evaluation, genetic parameters and fixed effects were estimated for birth weight (PN), for adjusted weight at 30 d (P30) and at 90 d (P90). Pedigree records (n=16,883) and weighing records (nPN=9,198; nP30=5,799; nP90=2,429) from LGMA of 362 litters were compiled. Genetic parameters and fixed effects, genetic values and precisions were predicted for PN, P30 and P90, through BLUP, with a mixed model including fixed effects of breeder × year of birth, season of birth, sex, age of mother (linear / quadratic effect) and as random effects genetic value of animal, maternal genetic effect and permanent environmental effect of litter. Fixed effects of prolificacy and number of weaned piglets were included as covariates, respectively, in PN and P30/P90 analyses. Mean values for PN 1.32±0.28 kg, 7.16±1.6 kg P30 and 34.1±6.4 kg P90 were recorded. For PN, P30 and P90, respectively, a heritability for direct effects of 0.170±0.156, 0.145±0.161 and 0.293±0.175; a maternal heritability of 0.084±0.109, 0.124±0.173 and 0.250±0.186 and a genetic correlation between direct and maternal effects of -0.344, -0.524 and -0.174 was estimated. Permanent environmental effect of litter was 0.395±0.056 PN, 0.403±0.086 P30 and 0.129±0.047 P90. In fixed effects, a superiority was observed for males of +0.031 kg PN, +0.046 kg P30 and +0.201 P90. Breeder × year effect showed maximum differences of 0.93, 5.21 and 19.25 kg for PN, P30 and P90, respectively, with Spring being the best season of birth. Sow age at farrowing had a quadratic effect for all traits. For each increase of 1 piglet in prolificacy, PN, P30 and P90 were reduced by 4, 7 and 58 g, respectively.info:eu-repo/semantics/publishedVersio

A comparative study of extraction apparatus in HPLC analysis of ochratoxin A in muscle

Abstract Ochratoxin A (OTA) is a secondary fungal metabolite produced by several moulds, mainly by Aspergillus ochraceus and by Penicillium verrucosum, that occurs in meat products. The aim of this work was to optimize an efficient extraction procedure for the determination of OTA in muscle tissue in order to assess its occurrence in meat samples. Three different apparatus, a Waring blender, a switching apparatus, and an ultrasonic processor, were evaluated to verify the efficiency of extraction. The analytical methods proposed involve the extraction with chloroform-orthophosphoric acid, cleanup through an immunoaffinity column, high-performance liquid chromatography/fluorescence detection for separation and identification of OTA, and confirmation with liquid chromatography/FD after methylation of OTA in muscle tissue. The limit of quantification of the proposed method was 0.04 µg kg-1. Recoveries of OTA, using switching apparatus, ranged from 90.3 to 103.2% for chicken muscle spiked at 2.4 and 0.48 µg kg-1, respectively, with a within-day relative standard deviation of 17 and 15.3%. The proposed method was applied to 38 chicken, swine, and turkey muscle samples and the presence of OTA was confirmed in five samples. Finally, the estimated daily intake of OTA in this study was between 23 pg kg-1 body weight per day for swine samples and 18 pg kg-1 body weight per day for turkey samples

Desenvolvimento de comunicação estratégica sobre biossegurança de plantas geneticamente modificadas: o caso do projeto LAC-Biosafety no Brasil.

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Efficacy And Safety Of Implantable Loop Recorder: Experience Of A Center

Introduction: Symptoms like syncope or palpitations frequently present a diagnostic challenge. An implantable loop recorder (ILR) is an important aid in the management of these patients. Methods: A retrospective study of patients that underwent ILR implantation from November 2007 to 2014. For each patient the indication for implantation, baseline characteristics, previous study, complications, recorded tracing and interventions were evaluated. Results: A total of 62 patients were included, 50% men, with a mean age of 62.5±18.8 years old. Previously to ILR implantation 88.7% of patients had performed Holter, 17.7% external events recorder, 33.9% Tilt test and 29% an electrophysiological study. The implantation indications were recurrent syncope in 90.3%, palpitations 8.1% and ischemic stroke in one patient. Mean follow-up time was 17.1±16.3 months. Symptoms were reported in 66.1% of the patients, 46.8% of those yielding a diagnostic finding. In all cases of palpitation complaints with diagnosis we found atrial fibrillation (AF). In patients with syncope atrioventricular conduction disturbance was demonstrated in 19.6%, sinus node dysfunction in 16.1%, paroxysmal supra-ventricular tachycardia 7.1% and AF in 1.8%. These finding resulted in 19 pacemaker and one CRT-D implantation, introduction of anticoagulation in five patients and one ablation of accessory pathway. There were no major complications. Conclusion: ILR proved to be safe and efficient. It has enabled the identification or exclusion of serious rhythm disturbances in more than half of patients and provided a targeted therapeutic intervention.info:eu-repo/semantics/publishedVersio

Efeito de doses de fosforo e fontes de potassio na produtividade e qualidade comercial da batata (Solanum tuberosum, L., cv. Achat).

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Melhoramento do feijão ao alcance de todos: I. cruzamentos.

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Vinte anos de integração pesquisa-extensão-agricultor com feijão no Rio Grande do Sul.

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