The Use of Sunn Hemp as Forage in Florida

Sunn hemp (Crotalaria juncea L.) is a warm-season annual legume that has been widely used as cover crop; however, there is potential to use sunn hemp as forage in subtropical regions. The objective of this study was to evaluate management practices to improve the efficiency of using sunn hemp as a forage crop. The study was conducted in Ona, Florida, USA from 2016 to 2018 and tested the factorial arrangement of five sunn hemp genotypes (Crescent Sunn, Ubon, Blue Leaf, and AU Golden) and two harvest times (60 d after seeding or flowering) with four replicates. There was a cultivar × harvest period effect on HA, crude protein (CP), and in vitro digestible organic matter (IVDOM) concentrations. Herbage accumulation was greater at flowering than 60 d harvest for all cultivars; however, the magnitude of increase was greater for Blue Leaf and Crescent Sun than AU Golden and Ubon. AU Golden and Ubon flowered at 83 and 92 d after seeding, while Blue Leaf and Crescent Sun flowered or were harvested at 166 d after seeding. AU Golden and Crescent Sunn had the greatest CP at 60 d harvest, and Blue Leaf and Ubon had the least CP concentrations. AU Golden and Crescent Sun had the greatest IVDOM at 60-d harvest; however, AU Golden and Ubon had greater IVDOM than Blue Leaf and Crescent Sun at flowering. Sunn hemp may be a feasible warm-season annual legume to be used in forage systems in Florida and harvest 60 d after seeding would results in forage with greater nutritive value

Energy demand and distribution of corn seeds as a function of the speed of two seedrills

This work had the objective of evaluating the distribution uniformity of corn seed in two displacement speeds and two precision seedrills, in an experiment conducted in a Oxisol, with slightly inclined relief in a loamy soil. The experimental design was constituted of four treatments composed of two seeders (S1 - PSE 8/Semeato and S2 - PST 2/Super Tatu) and two velocity of seedrills (V1 = 5.2 and V2 = 8.1 km h-1). The mean value of the traction force in the bar was 9.6 kN for S1 and 8.07 kN for S2, while the slippage was 5.83 and 4.26%, for S1 and S2, respectively. The values obtained for distribution uniformity of seeds of the seeder S1 in the speeds V1 and V2 were 21.6 and 22.0, and for the seeder S2 18.0 and 19.0 cm respectively, which did not differ statistically; however for the two displacement speeds a tendency of increase of the spacing among plants with the speed increase was verified.Objetivou-se, através deste trabalho, avaliar a uniformidade de distribuição de sementes de milho em duas velocidades de deslocamento de duas semeadoras-adubadoras de precisão, em experimento conduzido em um Latossolo Vermelho distroférrico, de relevo levemente inclinado e textura muito argilosa. O delineamento experimental constituiu-se de quatro tratamentos compostos de duas semeadoras (S1-PSE 8/Semeato, S2-PST 2/Super Tatu) e duas velocidades de deslocamento (V1 = 5,2 e V2 = 8,4 km h-1). O valor médio da força de tração na barra foi de 9,6 kN para S1 e 8,07 kN para S2, enquanto a patinagem foi de 5,83 e 4,26%, para S1 e S2, respectivamente. Os valores obtidos para o espaçamento entre sementes da semeadora S1 nas velocidades V1 e V2, foram 21,6 e 22,0 e, para a semeadora S2, 18,0 e 19,0 cm respectivamente, os quais não diferiram estatisticamente entre si; verificou-se, entretanto, para as duas velocidades de deslocamento, tendência de aumento dos espaçamentos entre plantas, com o aumento da velocidade.42442

Superação da dormência de Sementes de erva-mate (ilex paraguariensis st. hill) por estratificação

Sementes de erva-mate apresentam dormência e necessitam de tempo para superação e amadurecimento do embrião. Buscou-se reduzir o tempo da germinação para produção de mudas por sementes e subsidiar a estratificação como meio de superação da dormência. As sementes foram beneficiadas manualmente e, posteriormente, foi realizada a estratificação usando-se caixas plásticas com areia umedecida em água destilada, intercalando uma camada de areia (1 cm de altura) com uma de 100 sementes, totalizando seis camadas de sementes. Para realizar o experimento, foram adotados os seguintes tratamentos: permanência em germinador (25ºC), refrigerador (-2ºC), congelador (-12ºC) e testemunha em laboratório, por seis meses. Mensalmente retirou-se uma camada de sementes, as quais eram avaliadas quanto à germinação, e, em seguida, as sementes duras eram levadas ao germinador com nova avaliação a cada 30 dias. As médias foram comparadas pelo teste de comparação de duas proporções. As sementes submetidas ao germinador sofreram maior deterioração e as mantidas no refrigerador apresentaram melhor conservação. Verificou-se a ocorrência de germinação a partir do quinto mês de estratificação no tratamento germinador, porém sem diferença significativa, o que pode ter ocorrido devido à necessidade de maior tempo para estratificação. No entanto, observou-se influência na qualidade das sementes

New volatile constituents from leaves of Stemodia trifoliata (Link.) Reichb. (Schrophulariaceae)

The leaf essential oils of Stemodia trifoliata (Scrophulariaceae), collected at the same month of two different years (August 2005 and 2006), were analized by GC-MS and GC-FID. A total of 22 volatile components represented by sesquiterpenes and diterpenes was identified. β-Caryophyllene (9.4-15.4%) and caryophyllene oxide (6.2-9.0%) were the major compounds identified in the sesquiterpene fraction, while the diterpenoids 6α-acetoxymanoyl oxide (13.9-23.2%) and 6α-hydroxymanoyl oxide (25.1-29.7%) were the main constituents of the diterpene fraction. The two novel manoyl oxide derivatives had their structures established by means of spectroscopic methods, particularly 1D and 2D NMR. This work describes for the first time the chemical investigation on the volatile composition of S. trifoliata

Ecosystem Services Provided by Overseeding Aeschynomene Into Bahiagrass Pastures in South Florida

Aeschynomene (Aeschynomene americana L.) is a warm-season annual legume commonly overseeded into warm-season perennial grass pastures in tropical and subtropical regions. Although aeschynomene usually increases forage production and nutritive value, there is limited information about the ecosystem services provided by this legume. The objective of these studies was to evaluate the effects of overseeding aeschynomene into bahiagrass (Paspalum notatum Flüggé) pastures on nutrient dynamics and microbial N-cycling gene abundances. The studies were conducted in Ona, FL, from April to March 2019-2020 and 2020-2021. Treatments were the split-plot arrangement of two forage systems treatments (overseeding aeschynomene into bahiagrass or bahiagrass monoculture; main plots) and two N fertilization levels [0 (control) and 60 kg N ha-1 ; sub-plot], distributed in a randomized complete block design with four replicates. Forage characteristics were evaluated 8-wk after seeding and every 35 d thereafter. The static chamber technique was used to estimate nitrous oxide (N2O) emissions. The litter bag technique was used to estimate organic matter (OM) and N decomposition. Nitrogen-cycling gene abundances were measured by qPCR. Bahiagrass-aeschynomene had greater crude protein concentration than bahiagrass monoculture but there was no difference in forage accumulation. Nitrogen fertilization increased forage accumulation and daily N2O emissions. Litter from bahiagrass-aeschynomene had greater OM and N decomposition rates than bahiagrass only, and N fertilization did not affect litter decomposition. There were no differences in N-cycling microbial gene abundances among treatments. Overseeding aeschynomene into bahiagrass may provide additional ecosystem services, but the magnitude is conditional to the establishment and proportion of aeschynomene in the pasture botanical composition

Vitamin D3 as adjuvant in the treatment of type 2 diabetes mellitus: modulation of genomic and biochemical instability

Erratum in - Corrigendum: Vitamin D3 as adjuvant in the treatment of type 2 diabetes mellitus: modulation of genomic and biochemical instability. Fagundes GE, Macan TP, Rohr P, Damiani AP, Da Rocha FR, Pereira M, Longaretti LM, Vilela TC, Ceretta LB, Mendes C, Silveira PCL, Teixeira JPF, de Andrade VM. Mutagenesis. 2019 May 29;34(2):215. doi: 10.1093/mutage/gez006.Type 2 diabetes mellitus has undergone a worldwide growth in incidence in the world and has now acquired epidemic status. There is a strong link between type 2 diabetes and vitamin D deficiency. Because vitamin D has beneficial effects on glucose homeostasis, the aim of this study was to evaluate the influence of vitamin D3 supplementation on the modulation of glycaemic control and other metabolic effects, as well as modulation of genomic instability in patients with type 2 diabetes. We evaluated 75 patients with type 2 diabetes, registered in the Integrated Clinics of the University of Southern Santa Catarina. Participants received 4000 IU of vitamin D3 (25(OH)D) supplementation daily for 8 weeks. Blood samples were collected at the beginning and at the end of the supplementation, and 4 weeks after the end of supplementation. The glycidic and lipid profiles [total cholesterol, high-density lipoprotein (HDL), low-density lipoprotein and triglycerides], oxidative stress, DNA damage and 25(OH)D levels were evaluated. Vitamin D3 supplementation for 8 weeks showed enough to significantly increase blood levels of 25(OH)D. A significant difference in lipid profile was observed only in non-HDL cholesterol. Significant changes were observed in glucose homeostasis (fasting glucose and serum insulin) and, in addition, a reduction in the parameters of oxidative stress and DNA damage. There was a significant reduction in the values of 25(OH)D 4 weeks after the end of the supplementation, but levels still remained above baseline. Use of vitamin D supplementation can be an ally in the health modulation of patients with type 2 diabetes mellitusThis work was supported by grants from Conselho Nacional de Pesquisa e Desenvolvimento (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à Pesquisa e Inovação do Estado de Santa Catarina (FAPESC) and Programa de Pós-Graduação em Ciências da Saúde/ Universidade do Extremo Sul Catarinense.info:eu-repo/semantics/publishedVersio

Hepatitis C in Brazil: lessons learned with boceprevir and telaprevir

In 2012, the first-generation protease inhibitors telaprevir (TVR) and boceprevir (BOC) were introduced in the Brazilian health system for treatment of chronic hepatitis C, after their approval by the National Committee for Health Technology Incorporation (CONITEC). However, these medicines were discontinued in 2015. The short period of use in therapy and their high cost require a discussion about the consequences for patients and for the health system of the early incorporation of new therapies. The article presents a qualitative analysis of the incorporation process of both medications in Brazil and the results of a multicenter study that included patients treated with BOC or TVR between January 2011 and December 2015 in five Brazilian cities. The study included 855 patients (BOC: n=247) and (TVR: n=608). The document analysis showed that CONITEC’s decision to incorporate BOC and TVR was based on results of phase III clinical trials that compared sustained virologic response (SVR) rates of patients treated with BOC and TVR with rates of those that received placebo. However, these studies included a low percentage of cirrhotic patients. The SVR rates observed in this multicenter study were worse than clinical trials pointed out (BOC: 45.6%; TVR: 51.8%), but similar to those achieved with previously adopted therapies. The discontinuation rate due to adverse events was (BOC: 15.4%; TVR: 12.7%). Based on these unsatisfactory results, the study brings a discussion that goes beyond the therapy outcomes, exploring the incorporation of these high-cost medicines and the related decision-making process, contributing to future decisions in medicine policies and in the treatment of chronic hepatitis C