Intra and inter-rater reliability study of pelvic floor muscle dynamometric measurements

The aim of this study was to evaluate the intra and inter-rater reliability of pelvic floor muscle (PFM) dynamometric measurements for maximum and average strengths, as well as endurance. A convenience sample of 18 nulliparous women, without any urogynecological complaints, aged between 19 and 31 (mean age of 25.4±3.9) participated in this study. They were evaluated using a pelvic floor dynamometer based on load cell technology. The dynamometric evaluations were repeated in three successive sessions: two on the same day with a rest period of 30 minutes between them, and the third on the following day. All participants were evaluated twice in each session; first by examiner 1 followed by examiner 2. The vaginal dynamometry data were analyzed using three parameters: maximum strength, average strength, and endurance. The Intraclass Correlation Coefficient (ICC) was applied to estimate the PFM dynamometric measurement reliability, considering a good level as being above 0.75. The intra and inter-raters' analyses showed good reliability for maximum strength (ICCintra-rater1=0.96, ICCintra-rater2=0.95, and ICCinter-rater=0.96), average strength (ICCintra-rater1=0.96, ICCintra-rater2=0.94, and ICCinter-rater=0.97), and endurance (ICCintra-rater1=0.88, ICCintra-rater2=0.86, and ICCinter-rater=0.92) dynamometric measurements. The PFM dynamometric measurements showed good intra- and inter-rater reliability for maximum strength, average strength and endurance, which demonstrates that this is a reliable device that can be used in clinical practice.1929710

Leaf Appearance and Elongation in Panicum maximum cv. Tanzania Tillers of Varying Ages

A sward may be considered as a tiller population of varying ages and sizes, and these different age groups are likely to present distinct behaviour in terms of growth and herbage production. However, there is very little information on how tiller age, in association with grazing management practices (e.g. frequency and intensity of grazing), alter morphogenetic characteristics and, therefore, herbage production. Against this background, the present experiment had the objective to evaluate leaf appearance and elongation in Panicum maximum cv. Tanzania tillers of different age groups when submitted to intermittent grazing regimes

Herbage Production of Tanzania Grass (\u3cem\u3ePanicum maximum\u3c/em\u3e cv. Tanzania) Submitted to Combinations of Frequencies and Intensities of Grazing by Cattle

Animal production from pastures is a complex process comprising three main stages: herbage growth, consumption by grazing animals and conversion into animal products (Hodgson, 1990). Utilisation is the stage where the grazier finds greater flexibility for management, probably because most processes related to harvest of the produced herbage by the grazing animals are very responsive to manipulation and control of defoliation practices. The objective of this study was to evaluate herbage production of a Panicum maximum cv. Tanzania pasture submitted to combinations of frequencies and intensities of grazing

Effect of different cleansers on the weight and ion release of removable partial denture: an in vitro study

OBJECTIVE: Removable partial dentures (RPD) require different hygiene care, and association of brushing and chemical cleansing is the most recommended to control biofilm formation. However, the effect of cleansers has not been evaluated in RPD metallic components. The aim of this study was to evaluate in vitro the effect of different denture cleansers on the weight and ion release of RPD. MATERIAL AND METHODS: Five specimens (12x3 mm metallic disc positioned in a 38x18x4 mm mould filled with resin), 7 cleanser agents [Periogard (PE), Cepacol (CE), Corega Tabs (CT), Medical Interporous (MI), Polident (PO), 0.05% sodium hypochlorite (NaOCl), and distilled water (DW) (control)] and 2 cobalt-chromium alloys [DeguDent (DD), and VeraPDI (VPDI)] were used for each experimental situation. One hundred and eighty immersions were performed and the weight was analyzed with a high precision analytic balance. Data were recorded before and after the immersions. The ion release was analyzed using mass spectrometry with inductively coupled plasma. Data were analyzed by two-way ANOVA and Tukey HSD post hoc test at 5% significance level. RESULTS: Statistical analysis showed that CT and MI had higher values of weight loss with higher change in VPDI alloy compared to DD. The solutions that caused more ion release were NaOCl and MI. CONCLUSIONS: It may be concluded that 0.05% NaOCl and Medical Interporous tablets are not suitable as auxiliary chemical solutions for RPD care

Desenvolvimento de processos cromatográficos para retenção de amônia em água produzida- relatório final

A extração do petróleo em áreas offshore é, em geral, acompanhada de água de formação, originária do reservatório. Esta água tem características oleosas com um alto teor de sais o que constitui um sério problema ambiental. O nível de concentração de amônia nesta água é superior ao limite máximo permitido para descarte. A principal dificuldade na remoção de amônia da água produzida é o alto nível de íons sódio e a presença de muitos outros íons interferentes encontrados nesta água. Este relatório apresenta a consolidação do estudo da remoção de amônia de água produzida utilizando zeólitas e adsorventes comerciais

Effects of cardiopulmonary bypass on propofol pharmacokinetics and bispectral index during coronary surgery

PURPOSE: Cardiopulmonary bypass is known to alter propofol pharmacokinetics in patients undergoing cardiac surgery. However, few studies have evaluated the impact of these alterations on postoperative pharmacodynamics. This study was designed to test the hypothesis that changes in propofol pharmacokinetics increase hypnotic effects after cardiopulmonary bypass. METHODS: Twenty patients scheduled for on-pump coronary artery bypass graft (group, n=10) or off-pump coronary artery bypass graft (group, n=10) coronary artery bypass grafts were anesthetized with sufentanil and a propofol target controlled infusion (2.0 µg/mL). Depth of hypnosis was monitored using the bispectral index. Blood samples were collected from the induction of anesthesia up to 12 hours after the end of propofol infusion, at predetermined intervals. Plasma propofol concentrations were measured using high-performance liquid chromatography, followed by a non-compartmental propofol pharmacokinetic analysis. Data were analyzed using ANOVA, considering p<0.05 as significant. RESULTS: After cardiopulmonary bypass, despite similar plasma propofol concentrations in both groups, bispectral index values were lower in the on-pump coronary artery bypass graft group. Time to extubation after the end of propofol infusion was greater in the on-pump coronary artery bypass graft group (334 ± 117 vs. 216 ± 85 min, p = 0.04). Patients undergoing cardiopulmonary bypass had shorter biological (1.82 ± 0.5 vs. 3.67 ± 1.15h, p < 0.01) and terminal elimination (6.27 ± 1.29 vs. 10.5h ± 2.18, p < 0.01) half-life values, as well as higher total plasma clearance (28.36 ± 11.40 vs.18.29 ± 7.67 mL/kg/min, p = 0.03), compared to patients in the off-pump coronary artery bypass graft group. CONCLUSION: Aside from the increased sensitivity of the brain to anesthetics after cardiopulmonary bypass, changes in propofol pharmacokinetics may contribute to its central nervous system effects

Carbon nanomaterials for the purification of antileukemic drugs

Getting older is the biggest risk factor for most fatal diseases, including cancer, heart disease and Alzheimer. To overcome such age-related society diseases, it is crucial to optimize the production and purification of biopharmaceuticals, such as nucleic acid-based products, antibodies and recombinant proteins and enzymes. Low cost production combined with high purity levels allow their routinely use by a widespread population. Continuous progresses have been made for the development of recombinant therapeutic enzymes. L-asparaginase (LA) is an antileukemic biopharmaceutical enzyme of current high-cost. LA is produced via fermentation and its purification usually comprises several steps that account up to 80% of its total production cost (1). This work aims to develop sustainable technologies to extract and purify LA. Reusable functionalized nanomaterials, namely carbon nanomaterials (CNTs), are used as cost-effective purification techniques for the target enzyme. Initially, the synthesis and modification of CNTs was performed. Different CNTs were obtained and used for the purification of LA. Commercial LA was used for the first purification tests, in order to understand the behaviour of the enzyme in contact with the nanomaterial. Experimental conditions, such as pH, and material/LA ratio, contact time were optimized. LA activity was quantified by Nessler reaction (2). The first results reveal a total adsorption of LA by the CNTs. Depending on the CNT functionalization/ treatment, different values of recovered activity of LA were obtained. The modified CNTs are shown to be very promising nanomaterials for the purification of LA. The LA was easily attached to CNTs by adsorption under mild conditions. CNTs supports can be a real alternative for a single step immobilization/purification of LA.publishe

EFEITOS DE DOIS PROGRAMAS DE EXERCÍCIOS AQUÁTICOS NOS DISTÚRBIOS DO INTESTINO EM INDIVÍDUOS COM FIBROMIALGIA

Fibromyalgia is a chronic painful syndrome, characterized bydiffuse muscle skeletal pain, fatigue, sleep and bowel disturbance. This studyaimed to assess the effects of two different hydrotherapy programs &ndash; withactive exercises and passive relaxation exercises for the bowel disturbancesof subjects with fibromyalgia. Sixteen subjects with fibromyalgia concludedthe study. All of then related bowel symptoms, as constipation, flatulence,excrements with too much mucus and chronicle diarrhea. It was requestedthen to quantify the intensity of these symptoms, using analogous-numericalscale. Then, subjects were randomly assigned in three groups. The firstperformed active hydrotherapy exercises. The second, passive hydrotherapyrelaxation exercises. The third one was the control group. Exercises weredone for eight weeks. After that, subjects were reevaluated. When comparingbaseline and post-test scores, it was not detected any significant difference(p &gt; .05 for all the groups). It was not detected differences when comparingall the groups (p = .115). However, improvement was observed when the Gactivewas compared to the control group (p = 0,036). The program of active exercisesimproved the intensity of bowel disturbances of subjects with fibromyalgia.However, it is not possible to confirm that active exercises are more efficientthan relaxation exercises, even so the last one did not improved the boweldisturbances of these subjects.A fibromialgia &eacute; uma s&iacute;ndrome dolorosa cr&ocirc;nica, caracterizada pordor musculoesquel&eacute;tica difusa, fadiga, dist&uacute;rbios do sono e do intestino.Este trabalho objetiva analisar os efeitos de dois diferentes programas aqu&aacute;ticos&ndash; de exerc&iacute;cios ativos e de relaxamento passivo &ndash; nos dist&uacute;rbios dointestino de indiv&iacute;duos com fibromialgia. Dezesseis indiv&iacute;duos com a doen&ccedil;aconclu&iacute;ram o estudo. Todos relatavam queixas relacionadas ao intestino,como constipa&ccedil;&atilde;o, flatul&ecirc;ncia, fezes com muco excessivo e diarr&eacute;ia cr&ocirc;nica.Foi solicitado aos mesmos que quantificassem a intensidade destes sintomas,utilizando a escala an&aacute;logo-num&eacute;rica. Ap&oacute;s, foram aleatorizados emtr&ecirc;s grupos. O primeiro realizou exerc&iacute;cios aqu&aacute;ticos ativos (Gativo). O segundorealizou exerc&iacute;cios de relaxamento aqu&aacute;tico passivo (Grelax). O terceiroatuou como controle (Gcont). Os exerc&iacute;cios foram realizados por oito semanas,depois das quais os indiv&iacute;duos foram reavaliados. Quando se comparouo desempenho pr&eacute; e p&oacute;s intra-grupo, observou-se que nenhum dos gruposapresentou melhora significativa (p &gt; 0,05 para todos os grupos). A diferen&ccedil;atamb&eacute;m n&atilde;o ocorreu quando comparados todos os grupos (p = 0,115). Entretanto,observou-se melhora quando se comparou o Gativo e o grupo controle (p= 0,036). O programa de exerc&iacute;cios ativos melhorou a intensidade de dist&uacute;rbiosde intestino de indiv&iacute;duos com fibromialgia. Entretanto, n&atilde;o se podeafirmar que seja mais eficaz que o programa de exerc&iacute;cios de relaxamento,embora os &uacute;ltimos n&atilde;o tenham se mostrado eficazes em melhorar os dist&uacute;rbiosde intestino destes indiv&iacute;duos

Immobilization of L-asparaginase towards surface-modified carbon nanotubes

L-asparaginase (LA) is an enzyme that catalyzes L-asparagine hydrolysis into L-aspartic acid and ammonia and is mainly applied in pharmaceutical and food industries. The LA currently commercialized for pharmaceutical purposes is produced from two main bacterial sources: recombinant Escherichia coli and Erwinia chrysanthemi. However, some disadvantages are associated with its free form, such as the shorter half-life. Immobilization of LA has been proposed as an efficient approach to overcome this limitation. In this work, a straightforward method, including the functionalization of multi-walled carbon nanotubes (MWCNTs) through a hydrothermal oxidation treatment and the immobilization of LA by adsorption over pristine and modified MWCNTs was investigated. Different operation conditions, including pH, contact time, ASNase/MWCNT mass ratio, and the operational stability of the immobilized LA, were evaluated. The characterization of the LA-MWCNT bioconjugate was addressed using different techniques, namely Transmission Electron Microscopy (TEM), Thermogravimetric analysis (TGA), and Raman spectroscopy. Functionalized MWCNTs showed promising results, with an immobilization yield and a relative recovered activity of commercial LA above 95%, under the optimized adsorption conditions (pH 8, 60 min of contact, and 1.510–3 g.mL-1 of LA). The LA-MWCNT bioconjugate also showed improved enzyme operational stability (6 consecutive reaction cycles without activity loss), proving its suitability for application in industrial processes.publishe