Caso clínico bioquímico

Bisalbuminemia is a qualitative albumin variation defined by coexistence of two types of serum albumin with different electrophoretic mobilities in the same individual. It can be of two different types: hereditary (or permanent) and acquired (or transient). Herein is described a rare case of hereditary bisalbuminemia in a healthy infant, incidentally found during elevated aminotransferase study. Despite not having pathological significance, acknowledgement of this analytical alteration is key for adequate management of these patients.info:eu-repo/semantics/publishedVersio

Implementação do sistema de gestão de segurança alimentar

Mestrado em Biotecnologia AlimentarA presente dissertação apresenta a descrição das tarefas executadas ao longo do estágio curricular no ano letivo 2011/2012. O estágio e a dissertação permitirão concluir o Mestrado em Biotecnologia Alimentar. O trabalho teve o objetivo de rever e atualizar os conceitos teóricos, bem como apresentar as atividades desenvolvidas na “Empresa”, relacionadas com a implementação do Sistema de Gestão de Segurança Alimentar (SGSA) nos entrepostos, nomeadamente a todas as áreas alimentares: área de temperatura ambiente, área de congelação e área de refrigeração. Ao longo do estágio foram desenvolvidos os seguintes documentos: Manual de Gestão de Segurança Alimentar, Manual de Boas Práticas e o Programa de Pré-requisitos. Os documentos referentes ao plano de Análise de Perigos e Pontos Críticos de Controlo (HACCP), também foram elaborados: fluxogramas, quadros de descrição de etapas, análise de perigos, determinação de pontos críticos de controlo (PCC’s) e monitorização dos PCC’s. O plano de trabalhos proposto pelo orientador da “Empresa” foi cumprido, faltando a validação dos documentos elaborados e a formação dos colaboradores dos entrepostos.The present essay describes the tasks executed during the academic training in the academic year 2011/2012. Both the supervised training and the essay will enable me to finish the Master's degree in Food Biotechnology. The present work had as main goal to review and update the theoretical concepts, as well as to present the activities developed at the "Company", related to the implementation of the Food Security Management System (FSMS) at the wholesale suppliers, namely the food areas: room temperature, freezing and refrigeration area. During the supervised training it was developed the following documents: Handbook of Food Security Management, Good Practice Manual and the Prerequisites Program. It was also prepared the documents concerning the Hazard Analysis plan and Critical Control Points (HACCPs): flowcharts, phase description charts, Hazard Analysis, determination of Critical Control Points (CCPs) and monitoring CCPs. The work plan proposed by the "Company" supervisor was fulfilled, nevertheless the validation of the documents that have been prepared and the wholesale collaborators training are lacking

The moderating effect of parenting styles on the relationship between social anxiety and depressive symptomatology in adolescents

Intruduction: The comorbidity between depression and social anxiety is high in adolescence. Parental emotion socialization behaviors have been associated with the development of social skills and depressive symptomatology. Objectives: This study aims to explore the moderating effect of parenting styles on the relationship between social anxiety and depression, to study the associations between them, and to analyze the relationship between parenting styles, social anxiety and depressive symptomatology in adolescents. Methods: The sample consisted of 122 parents and their children. Self-report instruments were used to assess social anxiety, depressive symptomatology and parenting styles. Results: It was found that social anxiety is significantly associated to depression and that the former has a predictive effect on the latter. The parenting styles revealed no significant associations with either depressive symptomatology or with social anxiety, but a moderating effect of explorer parenting style was found in the relationship between social anxiety (public performance) and depressive symptomatology. Conclusions: The present investigation confirmed the existence of a significant association between social anxiety and depressive symptomatology in adolescence and suggests an effect of parental practices of emotional socialization in this relation, which, however, should be replicated in future research. It will also be important to study the effect of parenting styles on children's emotional regulation skills and their possible mediating effect on the relationship between social anxiety and depression.info:eu-repo/semantics/publishedVersio

Contribuição para o estudo molecular e morfológico de Espéccies do género Digitalis L.

Tese de mestrado, Medicamentos à Base de Plantas, Universidade de Lisboa, Faculdade de Farmácia, 2012O presente trabalho visa comprovar a hibridação natural entre Digitalis thapsi L. (endemismo ibérico) e Digitalis purpurea L. por meio de técnicas morfológicas e moleculares. Procedeu-se à caracterização morfológica através da microscopia ótica e eletrónica de varrimento e à análise molecular, através da técnica RAPD-PCR, sendo os resultados obtidos submetidos a tratamento estatístico usando para o efeito técnicas e programas informáticos adequados - SPSS e NTSYS-pc. Os resultados da análise macroscópica mostraram, terem os exemplares híbridos, folha e corola morfologicamente semelhantes às de D. thapsi, e cálice floral similar ao de D. purpurea. A corola destes exemplares híbridos é de coloração intermédia entre as duas espécies (D. thapsi e D. purpurea), tendo também os dois tipos de máculas característicos destas espécies sido observados nestes. A nível microscópico, os exemplares híbridos observados mostraram ter: a cutícula das células epidérmicas das folhas e flores e o tipo e forma dos tricomas secretores, semelhante aos observados em D. thapsi; e o tipo e forma dos tricomas tectores e número de camadas de células do parênquima lacunoso foliar semelhantes aos de D. purpurea. O parênquima em paliçada dos exemplares híbridos observados tem um número de camadas celulares intermédio entre as duas espécies (D. thapsi e D. purpurea). A observação e tratamento dos dados moleculares obtidos por RAPD-PCR mostrou terem os exemplares híbridos e D. thapsi um perfil molecular muito semelhante, sendo evidente a predominância dos caracteres desta espécie (D. thapsi) nestes, permitindo assim concluir serem os exemplares observados um híbrido D. thapsi x D. purpurea.The aim of this work was to confirm the natural hybridization between Digitalis thapsi (Iberian endemism) and Digitalis purpurea L. by means of morphological and molecular techniques. Proceeded to the morphological characterization by optical microscopy and scanning electronic and molecular analysis by RAPD-PCR, and the results analyzed statistically using for this purpose appropriate techniques and software - SPSS and NTSYS-pc. Macroscopic results revealed that the hybrid presents leaves and corolla similar to D. thapsi and floral cup similar to D. purpurea. Hybrid corolla appeared to be intermediate color and the two types of stains found in other species. The corolla of exemplary hybrid is intermediate in color between the two species (D. thapsi and D. purpurea), and also the two types of stains characteristic of these species were observed in these. At microscopic level, the samples proved to be hybrids observed: the cuticle of the epidermal cells of leaves and flowers and the type and form of secretory trichomes, similar to those observed in D. thapsi, and the type and shape of trichomes and number of layers of spongy parenchyma cells leaf similar to D. purpurea. The palisade parenchyma of the hybrid specimens have seen a number of cell layers intermediate between the two species (D. purpurea and D. thapsi). The observation and treatment of molecular data obtained by RAPD-PCR showed the specimens were hybrids and D. thapsi a molecular profile very similar, with a clear predominance of the characters of this specie (D. thapsi) in these, thus completing the specimens were observed a hybrid D. thapsi x D. purpurea

The effect of dental instruments on grip strength

Dentist work requires comfort and visibility, which sometimes causes an incorrect, maladjusted, and static posture, affecting mostly the upper limbs. It is due to prolonged work that oral health professionals are more susceptible to developing musculoskeletal disorders (MSDs) of the hand and wrist. The propose of this study was to evaluate and analyze the variation of grip strength in dental students. We also pretend to evaluate the incidence of signs and symptoms of MSDs. A clinical dental procedure was simulated using instruments of different design on phantoms to evaluate the palmar grip. Grip strength was measured with a dynamometer. The Nordic Musculoskeletal Questionnaire was conducted to assess the incidence of symptoms of MSDs. The most prevalent symptoms in this population were neck, lower back, and shoulder pain. During the procedure, 43 participants found the ergonomic curette more comfortable. After scraping with the metallic curette, a lower palmar grip strength was observed. Women have a higher incidence of MSDs and lower grip strength. Participants should transform and improve clinical habits to promote better working conditions and decrease MSDs. It was concluded that the grip force exerted during root scraping depends on the design of the curette. Instruments with a larger diameter and lower weight are more ergonomic and require less grip force.info:eu-repo/semantics/publishedVersio

Effect of phenolic compounds extracted from chestnut (Castanea sativa Mill) industry by-products on antibiotic resistant bacteria

In the last years, antibiotic resistance has become a selious threat tohuman and animal health since a large number of microorganisms have developed resistance to the drugs commonly used.Several natural compounds have been gcttingincreased attention as alternatives to many drugs. Phenolic compounds are secondary metabolites which exhibit several properties, such as, antimicrobial. antioxidant, anti-inflammatory, antimutagentic and cardioprotective. Thus, this study aims to investigate the antibacterial properties of the iJhenolic compounds extracted from the chestnut (Castaneasativa Mill.) industry by-products !gains! antibiotic resistant bacteria.info:eu-repo/semantics/publishedVersio

AVALIAÇÃO DA APRENDIZAGEM NA EDUCAÇÃO INFANTIL

Este artigo tem por objeto de estudo a avaliação da aprendizagem na educação infantil. O problema levantado caracteriza-se “Qual a importância da avaliação na Educação Infantil para o processo de aprendizagem das crianças?”. Apresentam-se como objetivos a discussão sobre a importância das práticas de avaliação para o processo de aprendizagem na Educação Infantil; analisar os principais instrumentos e metodologias utilizadas para avaliar as crianças na Educação Infantil; conhecer as principais dificuldades encontradas pelas professoras para avaliar as crianças. Para apreender o problema levantado, bem como atender aos objetivos propostos, utiliza-se a metodologia da pesquisa bibliográfica/exploratória na busca de materiais para leituras tais como livros e artigos em revistas científicas. O estudo emprega a pesquisa de campo com aplicação de questionário às professoras em três Centros Municipais de Educação Infantil de Quirinópolis-GO (CMEI). As análises tiveram como fundamentação teórica os trabalhos dos autores: Hoffmann (2017); Luckesi (2008), Silva (2002); Piaget (2010); Brasil (1996) dentre outros. A avaliação refere-se a um conjunto de atividades e procedimentos didático-pedagógicos que têm sua contribuição quanto ao desenvolvimento da criança de zero a cinco anos de idade. Consiste em uma ação reflexiva na melhoria das práticas pedagógicas em favor da aprendizagem na Educação Infantil. A pesquisa, segundo dados coletados esclarece que muitos profissionais, ainda, atentam para a observação classificatória, todavia o maior percentual dos entrevistados optam pela avaliação qualitativa por reconhecerem, mediante valores subjetivos, que é possível obter resultados satisfatórios quanto há um trabalho integrado de desenvolvimento da cognição/afetividade/psicomotor/cultural e social

Uso de antidepressivos nas regiões do Porto, Braga e Bragança

A depressão é uma das formas de perturbação mental mais frequente com uma prevalência na população geral de aproximadamente 10%. No que diz respeito ao tratamento desta patologia, a terapia a ser utilizada deve abranger a psicoterapia, mudanças no estilo de vida e a terapia farmacológica. Objetivo: Caraterizar o uso de antidepressivos nas regiões do Porto, Braga e Bragança. Metodologia: Este estudo é de caráter transversal, descritivo-correlacional, sustentado por um questionário distribuído por uma amostra de 385 indivíduos nas diferentes regiões. Para a edição e tratamento de dados foi utilizado o programa SPSS, versão 22. Resultados: Neste estudo participaram 385 indivíduos dos quais 38,96% pertencem ao sexo masculino e 61,04% ao sexo feminino. Relativamente ao uso de antidepressivos, 74,29% dos indivíduos afirmam que nunca tomaram antidepressivos. O Porto é a região que apresenta uma maior utilização de antidepressivos e a região de Bragança é a que apresenta um menor uso destes medicamentos. Discussão e Conclusão: Com a análise dos resultados foi possível concluir que os distritos do litoral, são aqueles onde há maior utilização de antidepressivos. Além disso, foi verificado que a utilização destes medicamentos está associada com a idade, o género e o estado civil. Constatou-se também que os antidepressivos mais utilizados foram a sertralina, o escitalopram e a fluoxetina. Apesar de bons resultados, o número de reações de adversas é alarmante, afetando mais de metade da população consumidora.info:eu-repo/semantics/publishedVersio

Hemangioendotelioma hepático infantil num recém-nascido

Infantile hepatic hemangioendothelioma is the most common benign hepatic vascular tumor in infants younger than six months and may undergo spontaneous regression during the first years of life. Diagnosis of these lesions in preterm newborns can be challenging, with its timing and accuracy relevant to avoid inappropriate therapeutic measures.info:eu-repo/semantics/publishedVersio

Screening of chemical composition, antimicrobial and antioxidant activities in pomegranate, quince, and persimmon leaf, peel, and seed: valorization of autumn fruits by-products for a one health perspective

Antimicrobial resistance is increasing globally and is now one of the major public health problems. Therefore, there is a need to search for new antimicrobial agents. The food industry generates large amounts of by-products that are rich in bioactive compounds, such as phenolic compounds, which are known to have several health benefits, including antioxidant and antimicrobial properties. Thus, we aimed to characterize the phenolic compounds present in pomegranate, quince, and persimmon by-products, as well as their antioxidant and antimicrobial activities. Phenolic compounds were extracted from pomegranate, quince, and persimmon leaves, seeds, and peels using a mixture of ethanol/water (80/20). The polyphenol profile of the extracts was determined by high-performance liquid chromatography. The antioxidant activity of the extracts was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and cupric reducing antioxidant capacity (CUPRAC) methods. Antimicrobial susceptibility was evaluated using the Kirby–Bauer disk diffusion method. In general, leaves showed higher concentrations of phenolics than the peel and seeds of fruits. In total, 23 phenolic compounds were identified and quantified, with sanguiin and apigenin-3-O-galactoside being present in the highest concentrations. Leaf extracts of pomegranate showed higher antioxidant activities than the other components in all methods used. In general, all extracts had a greater antimicrobial activity against Gram-positive bacteria. Persimmon leaf and seed extracts inhibited a greater number of bacteria, both Gram-positive and -negative. The lowest minimum inhibitory concentration (MIC) detected among Gram-positive and -negative bacteria was 10 mg/mL for pomegranate peel and leaf extracts against Staphylococcus aureus and S. pseudintermedius and for pomegranate leaf extract against Escherichia coli. Our results reinforce the need to value food industry by-products that could be used as food preservatives and antibiotic adjuvants against multiresistant bacteria.This work was supported by projects UIDP/00772/2020 and LA/P/0059/2020, funded by the Portuguese Foundation for Science and Technology (FCT), and by LAQV-REQUIMTE, which is financed by national funds from FCT/MCTES (UIDB/50006/2020 and UIDP/50006/2020). This work was also supported by National Funds from the FCT–Portuguese Foundation for Science and Technology, under project UIDB/04033/2020, as also to CIMO (UIDB/00690/2020 and UIDP/00690/2020), SusTEC (LA/P/0007/2020), and for L. Barros institutional contract; to FEDER through the Regional Operational Program North 2020, under the Project GreenHealth, Norte-01-0145-FEDER-000042.info:eu-repo/semantics/publishedVersio