Banana peel and grape stalk: potential of valorization through the evaluation of chemical composition and physical-chemical properties

CHISA 2008 - 18th International Congress of Chemical and Process Engineering (CD-ROM of Full Texts)Bioconversion of food processing residues (agr o-based industrial residues) in valuable products has been receiving an increasing attention in the last years. In fact, the main problem experienced by agro-based industries in several c ountries is the management of their residues. As a consequence, many research centers and government departments are preparing scientific strategies in order to develop biotechnological processes capable of transforming these residues in new bio-products or as sources of other chemicals. Most of these agro-industrial residues are lignocellulosic materials constituted basically of cellulose, hemicelluloses, and lignin. In particular, banana production is one of the main economic resources of several regions in many countries, an important crop in the tropical and sub- tropical regions and one of the most consumed fruits in the world. Grapes are other of the world’s largest fruit crops, mainly grown for direct consumption, grape juice and wine- making processes. These agricultural/industrial activities generate large amounts of residues such as banana fruit peel and grape stalk. Taking in consideration these facts, it is fundamental to know their chemical composition and physical-chemical properties, in order to evaluate perspectives of applications and improvement of procedures towards an efficient utilization of these residues. For this reason, following hydrolysis, the residues obtained were analyzed by HPLC, FTIR, TGA and DSC. Preliminary results indicate the attractiveness of these materials for further applications due to their chemical composition and physical-chemical properties: glucan and xylan contents of banana peel are 23.2 ± 0.2% and 18.9 ± 0.5%, respectively, while grape stalk contains 26.5 ± 1.5% and 16.8 ± 0.4%, respectively. These results are in agreement with those obtained with some other agro-industrial residues. These approaches offer several advantages, since the several fractions obtained from the hydrolysis of these annually produced materials can be applied as raw-materials to reduce the existence of environmentally hazardous situations and/or to increase the supply of energy or chemicals produced from renewable resourcesFCT(Portugal), FAPESP(Brazil) and CAPES/GRICES(Brazil/Portugal)info:eu-repo/semantics/publishedVersio

Herbage Accumulation and Animal Performance on Xaraes Palisade Grass Subjected to Intensities of Continuous Stocking Management

The large majority of species used in Brazil belong to the Brachiaria and Panicum genus, with marandu paladisadegrass (Brachiaria brizantha (Hochst. ex A. Rich) cv. Marandu) being the main cultivated forage grass species (Santos Filho 1996). Brachiaria brizantha cv. xaraés (xaraes palisade grass) was released as an option for diversifying forage species, and it had the advantages of fast regrowth and herbage yield, favouring larger stocking rates and animal productivity (Euclides et al. 2008; 2009). The objective of this study was to evaluate herbage accumulation and animal performance of beef cattle steers grazing continuously stocked xaraes palisade grass managed at 15, 30 and 45 cm

Meningoradiculitis Due To Cryptococcus Neofermans In An Immunocompetent Patient.

Meningoradiculitis refers to combined involvement of meninges and nerve roots. The most frequent location is the lumbosacral region. Etiology is diverse, including inflammatory, infectious and neoplastic disorders. Meningoradiculitis is a rare form of involvement in cryptococcal infection. We describe a case of subacute lower limbs flaccid paresis diagnosed as lumbosacral meningoradiculitis in view of cerebrospinal fluid (CSF) inflammatory changes and typical enhancement on MRI of lumbar spine. Cryptococcus neoformans was isolated from CSF. Extensive screening yielded no immunodeficiencies.62147-

Grazing Management Flexibility in Pastures Subjected to Rotational Stocking Management: Herbage Production and Chemical Composition of Kikuyu-Grass Swards

Several recent papers published on tropical pastures have pointed out that under rotational stocking management regrowth should be interrupted when canopy light interception is 95% (LI). Further, these studies have revealed a positive and high correlation between LI and sward height, allowing LI management targets to be defined in terms of sward height. However, there are some indications that lower pre-grazing heights relative to those targets would result in similar leaf accumulation without interfering with sward persistence. The objective of this paper was to verify a possible flexibility of such pre-grazing height targets. A replicated experiment was conducted with treatments corresponding to four pre-grazing height targets (25 cm, corresponding to a canopy light interception of 95%; 20; 15 and 10 cm), which were associated with a single severity of grazing equivalent to removal of 50% of initial height, leaving four post-grazing heights (12.5, 10.0, 7.5 and 5 cm, respectively). Preliminary results indicated that there were no differences in rate of herbage accumulation, herbage yield and crude protein, NDF and ADF contents on swards managed with the pre-grazing targets of 15, 20 and 25 cm. Swards managed with the 10 cm pre-grazing target had the highest contents of CP and lowest contents of NDF and ADF, but herbage accumulation was reduced. Overall, the findings indicate that there may be some flexibility in targets of pre-grazing sward height, provided that defoliation severity is moderate and does not interfere with herbage yield and quality. In that context, targets of pre-grazing sward height defined in terms of canopy light interception would correspond to the maximum value of the possible range of values to be used

Use of epidermic and granulocyte-colony stimulating growth factors in the prevention and treatment of necrotizing enterocolitis of the newborn

OBJETIVO: Revisar os conhecimentos existentes em relação ao uso de fatores de crescimento epidérmico e estimulador de colônias de granulócitos na prevenção e/ou no tratamento da enterocolite necrosante (ECN) durante o período neonatal. FONTES DE DADOS: Revisão da literatura, nas bases de dados Medline, Lilacs, SciELO e PubMed, utilizando os unitermos "recém-nascidos", "enterocolite" e "fatores de crescimento", no período de 2003 a 2007. Nesta busca, 49 artigos foram encontrados, sendo 17 pertinentes ao tema. Também foram utilizados outros artigos, independente do ano de publicação, relacionados a aspectos definidores da ECN no recém-nascido. SÍNTESE DOS DADOS: A ECN continua sendo responsável por uma elevada morbimortalidade neonatal. Os mecanismos fisiopatológicos vêm sendo elucidados e, a partir deles, são discutidas novas terapias, como o uso de fatores de crescimento, destacando-se o fator de crescimento epidérmico e o fator estimulador de colônias de granulócitos. CONCLUSÕES: O uso de fatores de crescimento no tratamento e prevenção da ECN neonatal parece promissor. É necessário maior número de ensaios clínicos para comprovar sua eficácia e segurança. Enquanto isso, a melhor prática médica continua sendo a prevenção da doença.OBJECTIVE: To review the literature regarding the use of hematopoietic and epidermic growth factors for prevention or treatment of neonatal necrotizing enterocolitis (NEC). DATA SOURCES: Literature review of Medline, Lilacs, SciELO and Pubmed databases, using the key-words "newborn", "enterocolitis" and "growth factors", from 2003 to 2007. Fourty-nine papers were retrieved, but only 17 related to the subject. Other studies that described some clinical aspects of enterocolitis were also included, regardless of the year of publication. DATA SYNTHESIS: Necrotizing and enterocolitis has been an important cause of morbidity and mortality in the neonatal period. As the knowledge about the pathophysiology of this disease improves, new therapies, such as the administration of epidermal growth factor and granulocyte colony-stimulating factor, are being discussed. CONCLUSIONS: The use of growth factors for treatment and prevention of NEC seems promising. However, further clinics assays are needed to evaluate the effectiveness and the safety of these growth factors. At this moment, the best clinical practice is the prevention of the disease

PARTNESHIP – A STRATEGY FOR HEALTH PROMOTION

Experiência de parceria do Núcleo de Convivência em Situações Crônicas de Saúde (NUCRON), a Associação Catarinenese de Ostomizados (ACO) e do Programa de Assistência ao Ostomizado (PAO). O processo de parceria iniciou em 1989, durante o qual foram conduzidos quatro projetos conjuntos, com enfoques na descoberta das potencialidades dos grupos e na expansão da ACO. A idéia de parceria foi criada para envolver os associados da ACO num processo de desenvolvimento de suas potencialidades individuais e coletivas e, com isso, fortalecer e expandir a ACO.The focus is on the partnership experience of the NUCRON (Nucleus ps Studies on chronical conditions.) ACO (Ostomized Association of the State of Santa Catarina) and the PAO (Program of Ostomized Patient Care). The partnership has been in progress since 1989, period in wich four research projects were conduted. The main partnership purpose was to envolve the ostomized patientes in a process os discovering their own potentialities in order to transform the ACO in a powerful Association

Experimental Chemotherapy for Chagas Disease: A Morphological, Biochemical, and Proteomic Overview of Potential Trypanosoma cruzi Targets of Amidines Derivatives and Naphthoquinones

Chagas disease (CD), caused by Trypanosoma cruzi, affects approximately eight million individuals in Latin America and is emerging in nonendemic areas due to the globalisation of immigration and nonvectorial transmission routes. Although CD represents an important public health problem, resulting in high morbidity and considerable mortality rates, few investments have been allocated towards developing novel anti-T. cruzi agents. The available therapy for CD is based on two nitro derivatives (benznidazole (Bz) and nifurtimox (Nf)) developed more than four decades ago. Both are far from ideal due to substantial secondary side effects, limited efficacy against different parasite isolates, long-term therapy, and their well-known poor activity in the late chronic phase. These drawbacks justify the urgent need to identify better drugs to treat chagasic patients. Although several classes of natural and synthetic compounds have been reported to act in vitro and in vivo on T. cruzi, since the introduction of Bz and Nf, only a few drugs, such as allopurinol and a few sterol inhibitors, have moved to clinical trials. This reflects, at least in part, the absence of well-established universal protocols to screen and compare drug activity. In addition, a large number of in vitro studies have been conducted using only epimastigotes and trypomastigotes instead of evaluating compounds' activities against intracellular amastigotes, which are the reproductive forms in the vertebrate host and are thus an important determinant in the selection and identification of effective compounds for further in vivo analysis. In addition, due to pharmacokinetics and absorption, distribution, metabolism, and excretion characteristics, several compounds that were promising in vitro have not been as effective as Nf or Bz in animal models of T. cruzi infection. In the last two decades, our team has collaborated with different medicinal chemistry groups to develop preclinical studies for CD and investigate the in vitro and in vivo efficacy, toxicity, selectivity, and parasite targets of different classes of natural and synthetic compounds. Some of these results will be briefly presented, focusing primarily on diamidines and related compounds and naphthoquinone derivatives that showed the most promising efficacy against T. cruzi

A CHARACTERISTIC OF LINKING RESEARCH PROJECT WITH NURSING CARE ACTIVITIES

Comunicamos uma experiência de processo envolvendo pesquisa, assistência e educação através da parceria do NUCRON, ACO e PAO com o propósito de mobilizar uma organização de grupos de pessoas ostomizadas e de profissionais da saúde para a intercomunicação de suportes para articulação em redes de serviços e associações. Utilizamos vários métodos e técnicas de trabalho tais como: mapeamento, seminários, questionários, reuniões, entrevistas, cursos, discussões em grupos. Os resultados permitiram: 1) visualização da distribuição dos núcleos do Estado de Santa Catarina onde os ostomizados recebem assistência; 2) Ter uma aproximação com a realidade vivenciada pelos ostomizados, e 3) conhecer o grande potencial do grupo de ostomizados e o desejo de implementar as várias decisões assumidas como compromisso o processo.The purpose of this study was to conduct a group organizational process with ostomized patients and health professionals in order to start an intercommunication system between the health service personal and the client association members. This experience has involved a research project, some education activities and a nursing care process. We have utilized various procedures such as: seminars, meetings, interview, questionairs and courses. The resoults showed the health facilities distribution; the ostomized patients reality and their potentialities for implementing the intercommunication process according to their undertaken decisions

Different Assay Conditions for Detecting the Production and Release of Heat-Labile and Heat-Stable Toxins in Enterotoxigenic Escherichia coli Isolates

Enterotoxigenic Escherichia coli (ETEC) produce heat-labile (LT) and/or heat-stable enterotoxins (ST). Despite that, the mechanism of action of both toxins are well known, there is great controversy in the literature concerning the in vitro production and release of LT and, for ST, no major concerns have been discussed. Furthermore, the majority of published papers describe the use of only one or a few ETEC isolates to define the production and release of these toxins, which hinders the detection of ETEC by phenotypic approaches. Thus, the present study was undertaken to obtain a better understanding of ST and LT toxin production and release under laboratory conditions. Accordingly, a collection of 90 LT-, ST-, and ST/LT-producing ETEC isolates was used to determine a protocol for toxin production and release aimed at ETEC detection. for this, we used previously raised anti-LT antibodies and the anti-ST monoclonal and polyclonal antibodies described herein. the presence of bile salts and the use of certain antibiotics improved ETEC toxin production/release. Triton X-100, as chemical treatment, proved to be an alternative method for toxin release. Consequently, a common protocol that can increase the production and release of LT and ST toxins could facilitate and enhance the sensitivity of diagnostic tests for ETEC using the raised and described antibodies in the present work.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Butantan Inst, Bacteriol Lab, BR-05503900 São Paulo, BrazilSão Paulo Trop Med Inst, Seroepidemiol & Immunol Lab, BR-05403000 São Paulo, BrazilFleury Med & Hlth, BR-04344903 São Paulo, BrazilButantan Inst, Immunopathol Lab, BR-05503900 São Paulo, BrazilButantan Inst, Immunochem Lab, BR-05503900 São Paulo, BrazilAdolfo Lutz Inst, Bacteriol Sect, BR-01246000 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol, BR-04923062 São Paulo, SP, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol, BR-04923062 São Paulo, SP, BrazilWeb of Scienc