Development of a continuous measurement system for atmospheric N2O and CO concentrations and its application to in-situ observation at Syowa Station, Antarctica

The Tenth Symposium on Polar Science/Ordinary sessions: [OM] Polar Meteorology and Glaciology, Wed. 4 Dec. / Entrance Hall (1st floor) , National Institute of Polar Researc

Printing surface charge as a new paradigm to program droplet transport

Directed, long-range and self-propelled transport of droplets on solid surfaces, especially on water repellent surfaces, is crucial for many applications from water harvesting to bio-analytical devices. One appealing strategy to achieve the preferential transport is to passively control the surface wetting gradients, topological or chemical, to break the asymmetric contact line and overcome the resistance force. Despite extensive progress, the directional droplet transport is limited to small transport velocity and short transport distance due to the fundamental trade-off: rapid transport of droplet demands a large wetting gradient, whereas long-range transport necessitates a relatively small wetting gradient. Here, we report a radically new strategy that resolves the bottleneck through the creation of an unexplored gradient in surface charge density (SCD). By leveraging on a facile droplet printing on superamphiphobic surfaces as well as the fundamental understanding of the mechanisms underpinning the creation of the preferential SCD, we demonstrate the self-propulsion of droplets with a record-high velocity over an ultra-long distance without the need for additional energy input. Such a Leidenfrost-like droplet transport, manifested at ambient condition, is also genetic, which can occur on a variety of substrates such as flexible and vertically placed surfaces. Moreover, distinct from conventional physical and chemical gradients, the new dimension of gradient in SCD can be programmed in a rewritable fashion. We envision that our work enriches and extends our capability in the manipulation of droplet transport and would find numerous potential applications otherwise impossible.Comment: 11 pages, 4 figure

Directional linearly polarized terahertz emission from argon clusters irradiated by noncollinear double-pulse beams

It has been demonstrated that the interaction between argon clusters and intense femtosecond double laser pulses with appropriate intervals in time and space provides important properties for terahertz electromagnetic wave generation, namely, high forward directivity, power enhancement, and linear polarization with a variable direction. Irradiating argon clusters with double pulses (1 and 3 mJ, 40 fs, 810 nm) in 133-ps and 40-μm intervals results in terahertz wave emission in the forward direction that is 10 times greater than that for a single pulse. The polarization direction of terahertz electromagnetic waves can be varied by changing the relative focal positions of the first and second pulses

Role of LOTR1 in nutrient transport through organization of spatial distribution of root endodermal barriers

The formation of Casparian strips and suberin lamellae at the endodermis limits the free diffusion of nutrients and harmful substances via the apoplastic space between the soil solution and the stele in roots [1–3]. Casparian strips are ring-like lignin polymers deposited in the middle of anticlinal cellwalls between endodermal cells and fill the gap between them [4–6]. Suberin lamellae are glycerolipid polymers covering the endodermal cells and likely function as a barrier to limit transmembrane movement of apoplastic solutes into the endodermal cells [7, 8].However, the current knowledge on the formation of these two distinct endodermal barriers and their regulatory role in nutrient transport is still limited. Here, we identify an uncharacterized gene,LOTR1, essential for Casparian strip formation in Arabidopsis thaliana. The lotr1 mutants display altered localization of CASP1, an essential protein for Casparian strip formation [9], disrupted Casparian strips, ectopic suberization of endodermal cells, and low accumulation of shoot calcium (Ca). Degradation by expression of a suberin-degrading enzyme in the mutants revealed that the ectopic suberization at the endodermal cells limits Ca transport through the transmembrane pathway, thereby causing reduced Ca delivery to the shoot. Moreover, analysis of the mutants showed that suberin lamellae function as an apoplastic diffusion barrier to the stele at sites of lateral root emergence where Casparian strips are disrupted. Our findings suggest that the transmembrane pathway through unsuberized endodermal cells, rather than the sites of lateral root emergence,mediates the transport of apoplastic substances such as Ca into the xylem

Detection and analyses by gel electrophoresis of cisplatin-mediated DNA damage.

DNA damage induced by cis-diamminedichloroplatinum (II) (cisplatin: cis-DDP), an anticancer drug, was studied in vitro by monitoring the drug-induced conformational change of pUC18 plasmid DNA, the sensitivity to some restriction enzymes of the damaged DNA and the sequence-dependent termination of DNA synthesis caused by cisplatin. Closed circular, superhelical pUC18 DNA was treated at 37 degrees C for 16 h with various concentrations of cisplatin. Cisplatin-dose-dependent conformational change due to unwinding of the treated DNA was detected by agarose gel electrophoresis. To analyze the base-specificity of the cisplatin damage, the measurement for sensitivity of cisplatin-treated DNA to various types of restriction enzyme and sequence gel analysis of the treated DNA were conducted. The results suggested that cisplatin attacked preferentially the sequence of GG &#62; AG &#62; GNG in the order. In the present assay condition, the cisplatin/DNA nucleotide ratios required for the DNA damage detection were roughly 0.025 for the conformational analysis, 0.001 or more for the restriction enzyme analysis, and less than 0.001 for the sequence gel analysis. By using the present method, it was demonstrated that the cisplatin-mediated DNA damage was inhibited by NaCl, KCl, CaCl2 or MgCl2 at their nearly physiological concentrations, and by reducing agents such as thiourea and 2-mercaptoethanol in the reaction mixture.</p

Inhibition of SIRT2 by Targeting GSK3β-Mediated Phosphorylation Alleviates SIRT2 Toxicity in SH-SY5Y Cells

Sirtuin 2 (SIRT2) is thought to be important in the pathogenesis of Parkinson’s disease (PD), and the inhibition of SIRT2 rescues α-synuclein toxicity in a cellular model of PD. Recent studies have focused on identifying inhibitors of SIRT2, but little is known about the processes that directly regulate its function. GSK3β is a serine/threonine protein kinase that affects a wide range of biological functions, and it is localized in Lewy bodies (LBs). Therefore, we investigated whether SIRT2 is regulated by GSK3β and enhances cell death in PD. In the present study, Western blot showed that total SIRT2 levels did not change noticeably in a cellular model of PD but that SIRT2 phosphorylation was increased, and GSK3β activity was elevated. In addition, mass spectrometry (MS) studies indicated that SIRT2 was phosphorylated by GSK3β at three specific sites. Phospho- or dephospho-mimicking studies demonstrated that this postmodification (phosphorylation) increased SIRT2 toxicity in SH-SY5Y cells. Collectively, our findings identify a posttranslational mechanism that controls SIRT2 function in PD and provide evidence for a novel regulatory pathway involving GSK3β, SIRT2, and α-synuclein

Near-Infrared Imaging Polarimetry of the Serpens Cloud Core: Magnetic Field Structure, Outflows, and Inflows in A Cluster Forming Clump

We made deep NIR imaging polarimetry toward the Serpens cloud core. The polarization vector maps enable us to newly detect 24 small IR reflection nebulae with YSOs. Polarization measurements of NIR point sources indicate an hourglass-shaped magnetic field, of which symmetry axis is nearly perpendicular to the elongation of the C18O (J=1-0) or submillimeter continuum emission. The bright part of C18O (J=1-0), submillimeter continuum cores as well as many class 0/I objects are located just toward the constriction region of the hourglass-shaped magnetic field. Applying the CF method, the magnetic field strength was estimated to be ~100 muG, suggesting that the ambient region of the Serpens cloud core is moderately magnetically supercritical. These suggest that the Serpens cloud core first contracted along the magnetic field to be an elongated cloud, which is perpendicular to the magnetic field, and that then the central part contracted cross the magnetic field due to the high density in the central region of the cloud core, where star formation is actively continuing. Comparison of this magnetic field with the previous observations of molecular gas and large-scale outflows suggests a possibility that the cloud dynamics is controlled by the magnetic field, protostellar outflows and gravitational inflows. This appears to be in good agreement with the outflow-driven turbulence model and implies the importance of the magnetic field to continuous star formation in the center region of the cluster forming region.Comment: 36 pages, 11 figures, 2 tables, accepted for publication in the Astrophysical Journa

Peplomycin-induced DNA repair synthesis in permeable mouse ascites sarcoma cells.

DNA repair synthesis induced in permeable mouse ascites sarcoma cells by peplomycin, an antitumor antibiotic, was studied. Mouse ascites sarcoma (SR-C3H/He) cells were permeabilized with a low concentration of Triton X-100 in an isotonic condition. Permeable cells were treated with an appropriate concentration of peplomycin to introduce single-strand breaks in permeable cell DNA. DNA repair synthesis in peplomycin-treated permeable cells was measured by incubating the cells with four deoxynucleoside triphosphates in an appropriate buffer system. The DNA repair synthesis was enhanced by ATP and NaCl at near physiological concentrations. More than 90% of DNA synthesis in the present system depended on the peplomycin-treatment. The repair nature of the DNA synthesis was confirmed by a BrdUMP density shift technique. The repair patches were largely completed and ligated in the presence of ATP. Analyses using selective inhibitors for DNA polymerases showed that both DNA polymerase Beta and aphidicolin-sensitive DNA polymerases (DNA polymerase alpha and/or delta) were involved in the repair DNA synthesis.&#60;/P&#62;</p