Rice production in Chitwan district of Nepal: An analysis from economic and environmental perspectives

Rice (Oryza sativa L.) is the major staple food in the Nepalese context. Chitwan district of Nepal was purposively selected to analyze the rice production from the socio-economic and environmental perspective. A total of 100 rice growing farmers, 50 organic and 50 inorganic were selected as the sample for the purpose of the study using the simple random method of sampling. Primary data were collected through a pre-tested semi-structure interview schedule and key informant interviews; secondary data were collected reviewing related publications. Descriptive statistics, multiple regression and chi-square test were used for data analysis. The multiple regression revealed that the four explanatory variables included in the model: age of the household head, primary occupation of the household head, number of family members involved in agriculture and subsidy in inputs for rice farming were found to have positive and statistically significant effect on rice yield (P<0.01). Moreover, chi-square test revealed that the farming practices that contributes to climate change mitigation such as: minimum tillage practice (P<0.05), crop diversification (P<0.01), green manuring (P<0.01), agro forestry practice (P<0.05), incorporating crop residues (P<0.1), weed management practice (P<0.01) and pest management practice (P<0.01)were found to be well adopted by the organic rice farmers, in contrast, the farming practices of inorganic rice farmers were statistically and significantly different in this respect. Government should make such policy that could grave the attention of the Nepalese people towards organic agriculture; moreover, encouraging them to make it their primary occupation

Effect of plastic mulches on growth and yield of potato (Solanum tuberosum L.) in Dadeldhura, Nepal

A field experiment was conducted from February to June, 2020 at Bhatkanda, Dadeldhura, Nepal to assess the effectiveness of plastic mulches in potato production. The experiment was laid out in Randomized Complete Block Design (RCBD) with four replications comprising of five treatments viz: T1: white plastic mulch (white on black colored), T2: silver plastic mulch (silver on black colored), T3: perforated black plastic mulch, T4: black plastic mulch and T5: control (without mulch). Results revealed that the black plastic mulch significantly increased the rate of emergence while perforated black plastic exhibited highest values of all other studied growth parameters, yield components and quality parameters. The highest marketable tuber yield was obtained in perforated black plastic (6.05 kg/m2) followed by silver plastic (5.62 kg/m2), white plastic (5.46 kg/m2), black plastic (5.14 kg/m2) and lowest marketable tuber yield was obtained in control condition (4.07 kg/m2). Similarly, temperature difference between controlled and mulched condition at 15 cm depth of soil was observed up to 2.8°C with its highest value in black plastic mulch and lowest in control condition. The perforated black plastic mulch was found most economical with maximum value of net return (NRs. 1904.31 thousands/ha) and B: C ratio (5.83). This study concludes that the use of perforated black plastic mulch is most economical with optimum plant growth and yield, producing best quality potatoes under climatic condition of Dadeldhura, Nepal

Low Viral Load Does Not Exclude Significant Liver Damage in Patients with Chronic HBV Infection in Bangladesh

Background: In general, it is assumed that patients with chronic hepatitis B virus (HBV) infection with high viral load exhibit increased liver damages. Accordingly, the treatment guidelines emphasize on reducing viral load in chronic HBV carriers. The ethical and scientific basis of these observations was mainly accumulated from investigations from developed countries of the world. More than 80% chronic HBV carriers live in the developing nations of the world, but little is known about relationship between HBV viral load and extent of liver damages in these countries. In this study, we addressed this issue to provide insights about this. Methods: In this retrospective study we reviewed the records of 210 chronic hepatitis B (CHB) patients from our pool of 561 Bangladeshi CHB patients. All of these 210 patients had low HBV DNA (<105 copies/ml by PCR). Of them 16 were HBeAg +ve and rest 194 HBeAg -ve. They have also been tested for other serologic markers of HBV (i.e. HBsAg, anti-HBe), HCV (i.e. anti-HCV) and serum alaninetransaminase (ALT) level. All patients also underwent per-cutaneous liver biopsy. Results: 37.5% (6/16) HBeAg +ve patients with low HBV DNA had significant hepatic necro-inflammation (HAI-NI ≥7), whereas this figure was 31.44% (61/194) in case of HBeAg -ve patients. On the other hand significant hepatic fibrosis (HAI-F ≥3) was observed in 31.25% (5/16) and 14.4% (28/194) in HBeAg +ve and -ve patients respectively. Conclusion: This study shows that a correlation could not be established between viral load and liver damage in patients with CHB in Bangladesh. A significant percentage of patients with low HBV DNA may have marked hepatic necro-inflammation and fibrosis, more so in case of HBeAg +ve CHB. Further study may be needed to find out the influence of other factors on liver damages in CHB patients in developing nations like Bangladesh, where about 8 million chronic HBV carriers are living. Most of these patients have not been characterized and treatment modalities have not been defined for them. Our study may suggest the research direction for management of these cases. Key Words: Low HBV DNA; Chronic hepatitis B; Hepatic necro-inflammation; Hepatic fibrosis.DOI: 10.3329/bsmmuj.v1i1.3693 BSMMU J 2008; 1(1): 19-2

Efficacy of Various Biopesticides against Red Ant (Dorylus orientalis Westwood) in Potato Field

Red ant (Dorylus orientalis Westwood) is the most destructive insect pest of potato tubers in the field in Nepal. A field experiment was conducted at Bhatkada, Dadeldhura district, Nepal in 2020 to determine the efficacy of various biopesticides and chemical insecticide on red ants. The plot size in this experiment was 106.7 m2. Five treatments viz. Chlorpyriphos 20% EC (2 mL L-1 water), Banmaara (2 kg plot-1), Cow urine (2 L 6 L-1water plot-1), Agave (25 g tuber-1) and Control (no treatment) were evaluated in randomized complete block design with four replications. The data on plant height and the number of leaves were taken at 45, 60, and 75 days after planting (DAP). The data on slightly and severely infested tubers were taken after harvesting of potato. The results showed that Chlorpyriphos treated plots gave the lowest values of traits viz numbers of slightly infested tuber (91,500 ha-1), severely infested tubers (12,500 ha-1), weights of slight infested tubers (7,700 kg ha-1) and severely infested tuber (1,130 kg ha-1). Among the botanical pesticides, Agave treated plots provided the second lowest values of numbers of slightly infested tuber (145,500 ha-1), severely infested tubers (37,400 ha-1), weights of slight infested tubers (13,400 kg ha-1) and severely infested tuber (3,110 kg ha-1). The results showed that the Agave treatment provided the highest marketable yield (50,900 kg ha-1). Thus, Chlorpyriphos and Agave could be used to efficiently protect potato tubers from red ant in potato field

Prevalence and risk of hepatitis E virus infection in the HIV population of Nepal

Background: Infection with the hepatitis E virus (HEV) can cause acute hepatitis in endemic areas in immune-competent hosts, as well as chronic infection in immune-compromised subjects in non-endemic areas. Most studies assessing HEV infection in HIV-infected populations have been performed in developed countries that are usually affected by HEV genotype 3. The objective of this study is to measure the prevalence and risk of acquiring HEV among HIV-infected individuals in Nepal. Methods: We prospectively evaluated 459 Human Immunodeficiency Virus (HIV)-positive individuals from Nepal, an endemic country for HEV, for seroprevalence of HEV and assessed risk factors associated with HEV infection. All individuals were on antiretroviral therapy and healthy blood donors were used as controls. Results: We found a high prevalence of HEV IgG (39.4%) and HEV IgM (15.3%) in HIV-positive subjects when compared to healthy HIV-negative controls: 9.5% and 4.4%, respectively (OR: 6.17, 95% CI 4.42-8.61, p \u3c 0.001 and OR: 3.7, 95% CI 2.35-5.92, p \u3c 0.001, respectively). Individuals residing in the Kathmandu area showed a significantly higher HEV IgG seroprevalance compared to individuals residing outside of Kathmandu (76.8% vs 11.1%, OR: 30.33, 95% CI 18.02-51.04, p = 0.001). Mean CD4 counts, HIV viral load and presence of hepatitis B surface antigen correlated with higher HEV IgM rate, while presence of hepatitis C antibody correlated with higher rate of HEV IgG in serum. Overall, individuals with HEV IgM positivity had higher levels of alanine aminotransferase (ALT) than IgM negative subjects, suggesting active acute infection. However, no specific symptoms for hepatitis were identified. Conclusion: HIV-positive subjects living in Kathmandu are at higher risk of acquiring HEV infection as compared to the general population and to HIV-positive subjects living outside Kathmandu

Prevalence and risk of hepatitis e virus infection in the HIV population of Nepal

Background: Infection with the hepatitis E virus (HEV) can cause acute hepatitis in endemic areas in immune-competent hosts, as well as chronic infection in immune-compromised subjects in non-endemic areas. Most studies assessing HEV infection in HIV-infected populations have been performed in developed countries that are usually affected by HEV genotype 3. The objective of this study is to measure the prevalence and risk of acquiring HEV among HIV-infected individuals in Nepal. Methods: We prospectively evaluated 459 Human Immunodeficiency Virus (HIV)-positive individuals from Nepal, an endemic country for HEV, for seroprevalence of HEV and assessed risk factors associated with HEV infection. All individuals were on antiretroviral therapy and healthy blood donors were used as controls. Results: We found a high prevalence of HEV IgG (39.4%) and HEV IgM (15.3%) in HIV-positive subjects when compared to healthy HIV-negative controls: 9.5% and 4.4%, respectively (OR: 6.17, 95% CI 4.42-8.61, p < 0.001 and OR: 3.7, 95% CI 2.35-5.92, p < 0.001, respectively). Individuals residing in the Kathmandu area showed a significantly higher HEV IgG seroprevalance compared to individuals residing outside of Kathmandu (76.8% vs 11.1%, OR: 30.33, 95% CI 18.02-51.04, p = 0.001). Mean CD4 counts, HIV viral load and presence of hepatitis B surface antigen correlated with higher HEV IgM rate, while presence of hepatitis C antibody correlated with higher rate of HEV IgG in serum. Overall, individuals with HEV IgM positivity had higher levels of alanine aminotransferase (ALT) than IgM negative subjects, suggesting active acute infection. However, no specific symptoms for hepatitis were identified. Conclusions: HIV-positive subjects living in Kathmandu are at higher risk of acquiring HEV infection as compared to the general population and to HIV-positive subjects living outside Kathmandu

Impact of milk protein type on the viability and storage stability of microencapsulated Lactobacillus acidophilus using spray drying

Three different milk proteins — skim milk powder (SMP), sodium caseinate (SC) and whey protein concentrate (WPC) — were tested for their ability to stabilize microencapsulated L. acidophilus produced using spray drying. Maltodextrin (MD) was used as the primary wall material in all samples, milk protein as the secondary wall material (7:3 MD/milk protein ratio) and the simple sugars, d-glucose and trehalose were used as tertiary wall materials (8:2:2 MD/protein/sugar ratio) combinations of all wall materials were tested for their ability to enhance the microbial and techno-functional stability of microencapsulated powders. Of the optional secondary wall materials, WPC improved L. acidophilus viability, up to 70 % during drying; SMP enhanced stability by up to 59 % and SC up to 6 %. Lactose and whey protein content enhanced thermoprotection; this is possibly due to their ability to depress the glass transition and melting temperatures and to release antioxidants. The resultant L. acidophilus powders were stored for 90 days at 4 °C, 25 °C and 35 °C and the loss of viability calculated. The highest survival rates were obtained at 4 °C, inactivation rates for storage were dependent on the carrier wall material and the SMP/d-glucose powders had the lowest inactivation rates (0.013 day−1) whilst the highest was observed for the control containing only MD (0.041 day−1) and the SC-based system (0.030 day−1). Further increase in storage temperature (25 °C and 35 °C) was accompanied by increase of the inactivation rates of L. acidophilus that followed Arrhenius kinetics. In general, SMP-based formulations exhibited the highest temperature dependency whilst WPC the lowest. d-Glucose addition improved the storage stability of the probiotic powders although it was accompanied by an increase of the residual moisture, water activity and hygroscopicity, and a reduction of the glass transition temperature in the tested systems

Effects of Climate Change on the Livestock Population in Mustang District, Nepal

There is strong evidence to suggest that climate change has affected and will continue to affect the occurrence, distribution, and prevalence of livestock diseases in Nepal. This study investigated how climate change has affected the livestock population in Mustang District. The outbreak of new diseases, changes in disease patterns, increase in cases of external and internal parasites, decrease in the availability of forage and fodder, and deterioration of pasture land were the major climate change effects observed. The number of livestock has decreased, resulting in declining income from livestockrelated activities of Jomsom and Kagbeni, two Village Development Committees in the Mustang District where people are seeking alternative occupations. The results revealed that some signs of climate change were experienced by rural communities in the study sites, such as an increasing number of warm days and a decreasing number of cold days. The different measures that people have adopted to lessen the impacts of climate change on the livestock population were also identified

A Unique Spontaneous Subcapsular Bilioma: Is it an idiopathic variety?

Spontaneous Subcapsular bilioma is an extrabiliary intrahepatic collection of bile in subcapsular location commonly occurring after biliary trauma or surgery. It rarely occurs in absence of any underlying biliary abnormality. We report a such a case with no known underlying biliary tract abnormality except for a solitary gall stone. We bring into discussion whether this type of bilioma can be labeled as idiopathic spontaneous variety in presence of gall stone. Keywords: bile; idiopathic; subcapsular bilioma