Imidazolines increase the levels of the autophagosomal marker LC3-II in macrophage-like RAW264.7 cells

This study evaluated whether imidazolines can induce autophagy in the murine macrophage-like cell line RAW264.7. Idazoxan increased the content of LC3-II, an autophagosomal marker, in RAW264.7 cells. To determine whether this effect was due to the induction of its synthesis or inhibition of its degradation, idazoxan-treatment was performed in the presence of bafilomycin A1, which blocks autophagosome-lysosome fusion, as well as Pepstatin A and E-64d, both of which block protein degradation in autolysosomes. An increased content of LC3-II was observed in the presence of bafilomycin A1 as well as the protease inhibitors. Furthermore, an increased number of autophagosomes was observed following idazoxan-treatment using an autophagosome-specific dye. This indicated that idazoxan induced autophagy. Other imidazolines, such as efaroxan, clonidine, and 2-(2-Benzofuranyl)-2-imidazoline, also increased the LC3-II content in RAW264.7 cells in the presence of bafilomycin A1. Taken together, these results indicate that some imidazolines, including idazoxan, can induce autophagy in RAW264.7 cells.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

A numerical experiment on the influence of the interannual variation of sea surface temperature on terrestrial precipitation in northern Japan during the cold season

The role of sea surface temperature (SST) anomaly in modulating the terrestrial precipitation in winter around Japan was investigated using a regional atmospheric model. Large amount of snowfall occurs as the westerly winter monsoon carries abundant moisture from the Japan Sea. An experiment with realistic SST gives improved representation of terrestrial precipitation distribution compared to the reanalysis. The standard deviation of interannual variation of precipitation in the experiment was approximately 20% larger over the Kuroshio extension than that in the experiment with climatology SST, suggesting that the SST variability enhances the oceanic precipitation variability. For the role of Japan Sea, the terrestrial precipitation over the Japan Sea side (JSS) region in northern Japan was sensitive to the offshore SST anomaly through affecting moisture flux toward Japan. Since the offshore SST was clearly warmer in the 2000s relative to the 1980s, the effect of the long-term SST variation on the terrestrial precipitation trend was examined. The experiment with realistic SST simulated the observed trend in terrestrial precipitation in the JSS region. In contrast, the precipitation trend was significantly reduced in the experiment with climatology SST. Therefore, the long-term SST trend is an important factor for the precipitation trend in the region of Japan and the adjacent oceans where SST has significant trends. Precipitation in southern Japan facing the Pacific Ocean indicated increasing trend even without the SST trend. This suggests that the long-term variations in extratropical cyclones are also an important factor for precipitation trends around the Kuroshio extension