Simple, Frequent Indicator for Personal Identification-Postmortem and Antemortem Abdominal Computed Tomography Findings of a Charred Body.

Postmortem personal identification in forensic science is performed using various methods. However, severely burnt bodies are hard to identify using odontological or skeletal features because of carbonization, and sometimes DNA profiling is impracticable because of the unavailability of the relatives. We present a case of a burn victim found after a house fire. Personal identification was attempted, but the body was heavily charred to the bones and the use of physical appearance was impracticable. There were no known relatives or personal belongings of the deceased for comparison of DNA typing. We obtained a series of abdominal computed tomography (CT) scans taken antemortem and found bilateral multiple renal cysts, left renal artery calcification, and a big right inguinal hernia, which matched the deceased\u27s postmortem CT findings and autopsy findings. To date, studies of identification by CT have acted for a rise in precision, but they require complicated calculation or high graphical methods. Calcification of the arteries or renal cysts seen in our case are very common lesions present in many adults with abundant variation; thus, they may be helpful as simple indicators for identification

Artificial Production and Natural Breeding of the Endangered Frog Species Odorrana ishikawae, with Special Reference to Fauna Conservation in the Laboratory

Odorrana ishikawae is listed as a class IB endangered species in the IUCN Red List and is protected by law in both Okinawa and Kagoshima Prefectures, Japan. Here, in an effort to help effectively preserve the genetic diversity of this endangered species in the laboratory, we tested a farming technique involving the artificial breeding of frogs, and also promoted natural breeding in the laboratory. Field-caught male/female pairs of the Amami and Okinawa Island populations were artificially bred using an artificial insemination method in the 2004, 2006, and 2008 breeding seasons (March to April). Although fewer than 50% of the inseminated eggs achieved metamorphosis, approximately 500, 300, and 250 offspring from the three respective trials are currently being raised in the laboratory. During the 2009 and 2010 breeding seasons, second-generation offspring were produced by the natural mating activities of the first offspring derived from the two artificial matings in 2004. The findings and the methods presented here appear to be applicable to the temporary protection of genetic diversity of local populations in which the number of individuals has decreased or the environmental conditions have worsened to levels that frogs are unable to survive by themselves

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Current Issues in Dental Identification in Severely Burned Bodies from a Studio Arson Attack in Japan

A studio arson attack in Japan caused 36 deaths. Dentists obtained dental findings from 33 unidentified bodies immediately after the attack and autopsy. The dental findings before autopsy were obtained on the day of the incident and the next day. Dental findings after autopsy were obtained at 1 week from the day after the incident. We examined issues associated with collecting these dental findings. Other findings recorded were CT images, autopsy findings, and various blood and pathological examinations. Among the 33 unidentified bodies, for 30 bodies, the number of teeth from which the dental findings were obtained was significantly higher after autopsy than before autopsy. The dental findings of the remaining three bodies were not obtained before or after the autopsy. Five percent of anterior teeth and 28.7% of molars had differing pre-autopsy and post-autopsy findings. This discrepancy can be attributed to various factors. One of the major influencing factors was the carbonization of the facial surface muscles and skin along with limited mouth opening due to thermocoagulation. For the scientific identification of an individual based on dental findings, it is mandatory to examine the teeth in all quadrants. The collection of dental findings solely from anterior teeth may not be adequate to establish identity. The present article describes the challenges faced by the dentists while identifying charred bodies in an arson attack

Application of Teeth in Toxicological Analysis of Decomposed Cadavers Using a Carbamazepine-Administered Rat Model

In a regular autopsy, blood and organs are used to quantify drug and toxicant concentrations; however, specimens such as blood cannot be collected from highly decomposed corpses, making the quantification of drug and toxicants impossible. This study aimed to estimate the blood carbamazepine (CBZ) concentration from teeth, a part of the human body that is best preserved after death. We sampled teeth and blood of rats administered CBZ. The correlation between the tooth and serum CBZ concentrations was analyzed. Rats were euthanized after CBZ administration and kept at 22 °C for 0 to 15 days before sampling the teeth and measuring the CBZ concentration. Undecalcified, fresh, frozen sections of rat teeth were prepared, and CBZ localization was evaluated. CBZ concentrations in both teeth and cardiac blood peaked at 60 min after administration and increased in a dose-dependent manner. CBZ concentration in teeth did not substantially change after death, with high CBZ distribution being observed in the pulp cavity. The tooth and serum CBZ concentrations were highly correlated, suggesting that the measurement of toxicant concentration in sampled teeth would allow for the estimation of blood toxicant concentration in highly decomposed corpses

琉球列島で得られた日本初記録のカワウミヘビLamnostoma mindorum (条鰭綱: ウナギ目: ウミヘビ科)

A single specimen (626 mm in total length) of Lamnostoma mindorum (Jordan & Richardson, 1908) was collected from the freshwater area in Okinawa Island, Ryukyu Islands, Japan. The specimen represents the first record of L. mindorum from Japan and the northernmost record of this species.沖縄島の淡水域においてカワウミヘビ (新称) Lamnostoma mindorum (Jordan & Richardson, 1908) 1個体（全長626 mm)を捕獲した. 本確認は本種の日本初記録であるとともに北限記録である