‘ZhongPan 101’ and ‘ZhongPan 102’: Two Flat Peach Cultivars From China

Flat peach [Prunus persica (L.) Batsch var. platycarpa] is a variant of ordinary peach with a unique flat shape. It is well known for its shape and delicious fruits (Miao et al. 2022). Although flat peach has a long history of cultivation in China, until the beginning of the 20th century, flat peach was only distributed as a minor variety in the main peach-producing areas of China. In terms of flat peach cultivars, only 46 of the 709 peach cultivars listed in Peach Genetic Resource in China (Wang et al. 2012) are flat peach cultivars, and most of them are flat landraces. Several problems have been noted previously in flat peach cultivars, including poor closure of the blossom end (blossom-end scarring in mild cases and cracking in severe cases), cracked stone in some cultivars (loss of commercial value in severe cases), nonsymmetrical fruit shape, small flesh, and low yield (Wang 2021). Many of the shortcomings of flat peach cultivars are intrinsic problems of the cultivars, which are difficult to improve through cultivation measures. This is the key factor limiting the large-scale promotion of flat peach cultivation in China. For many years, peach breeders in China have been devoted to the genetic improvement of flat peach, and some improved flat peach cultivars have been released, for instance, ‘Pocket Zaoban’ (Jiang et al. 2007) and ‘124 Pantao’ (Ma et al. 2003). However, problems persist in these cultivars, including small fruits, soft flesh, and blossom-end cracks. Only a few flat peach cultivars have good overall performance. In recent years, the Zhengzhou Fruit Research Institute (ZFRI), Chinese Academy of Agricultural Sciences (CAAS), identified genetic sources of flat peach with slow or nonmelting flesh, a well-closed blossom end, and little or no cracking. They were hybridized with high-quality peach and nectarine cultivars or selections. After multiple generations of improvement, breakthroughs were made in early flat peach breeding, and a series of flat peach cultivars with excellent comprehensive traits have been produced. These cultivars are favored by fruit farmers in the main peach-producing areas in China. Hence, the main problems in flat peach cultivation are expected to be solved, which will help expand the cultivation area of flat peach. ‘ZhongPan 101’ and ‘ZhongPan 102’ are two yellow-flesh flat peach cultivars 45 released from the ZFRI, CAAS. These two cultivars produce large, well-shaped, high-quality fruits with a completely closed stylar end and high yield. Three years of evaluation has confirmed that the peach trees of the two cultivars are stable. ‘ZhongPan 101’ and ‘ZhongPan 102’ were well adapted to climate of the middle and lower reaches of the Yellow River; have performed well in Henan, Jiangsu, and Anhui Provinces; and are suggested for trial wherever ‘ZhongYouPan 9’ is grown

Associations of vitamin D-related single nucleotide polymorphisms with post-stroke depression among ischemic stroke population

ObjectiveTo investigate the relationship between single nucleotide polymorphisms (SNPs) related to vitamin D (VitD) metabolism and post-stroke depression (PSD) in patients with ischemic stroke.MethodsA total of 210 patients with ischemic stroke were enrolled at the Department of Neurology in Xiangya Hospital, Central South University, from July 2019 to August 2021. SNPs in the VitD metabolic pathway (VDR, CYP2R1, CYP24A1, and CYP27B1) were genotyped using the SNPscan™ multiplex SNP typing kit. Demographic and clinical data were collected using a standardized questionnaire. Multiple genetic models including dominant, recessive, and over-dominant models were utilized to analyze the associations between SNPs and PSD.ResultsIn the dominant, recessive, and over-dominant models, no significant association was observed between the selected SNPs in the CYP24A1 and CYP2R1 genes and PSD. However, univariate and multivariate logistic regression analysis revealed that the CYP27B1 rs10877012 G/G genotype was associated with a decreased risk of PSD (OR: 0.41, 95% CI: 0.18–0.92, p = 0.030 and OR: 0.42, 95% CI: 0.18–0.98, p = 0.040, respectively). Furthermore, haplotype association analysis indicated that rs11568820-rs1544410-rs2228570-rs7975232-rs731236 CCGAA haplotype in the VDR gene was associated with a reduced risk of PSD (OR: 0.14, 95% CI: 0.03–0.65, p = 0.010), whereas no significant association was observed between haplotypes in the CYP2R1 and CYP24A1 genes and PSD.ConclusionOur findings suggest that the polymorphisms of VitD metabolic pathway genes VDR and CYP27B1 may be associated with PSD in patients with ischemic stroke

Dynamic residual deep learning with photoelectrically regulated neurons for immunological classification

Dynamic deep learning is considered to simulate the nonlinear memory process of the human brain during long-term potentiation and long-term depression. Here, we propose a photoelectrically modulated synaptic transistor based on MXenes that adjusts the nonlinearity and asymmetry by mixing controllable pulses. According to the advantage of residual deep learning, the rule of dynamic learning is thus elaborately developed to improve the accuracy of a highly homologous database (colorimetric enzyme-linked immunosorbent assay [c-ELISA]) from 80.9% to 87.2% and realize the fast convergence. Besides, mixed stimulation also remarkably shortens the iterative update time to 11.6 s as a result of the photoelectric effect accelerating the relaxation of ion migration. Finally, we extend the dynamic learning strategy to long short-term memory (LSTM) and standard datasets (Cifar10 and Cifar100), which well proves the strong robustness of dynamic learning. This work paves the way toward potential synaptic bionic retina for computer-aided detection in immunology

The role of indoleamine 2,3-dioxygenase 1 in early-onset post-stroke depression

BackgroundThe immune-inflammatory response has been widely considered to be involved in the pathogenesis of post-stroke depression (PSD), but there is ambiguity about the mechanism underlying such association.MethodsAccording to Diagnostic and Statistical Manual of Mental Disorders (5th edition), depressive symptoms were assessed at 2 weeks after stroke onset. 15 single nucleotide polymorphisms (SNPs) in genes of indoleamine 2,3-dioxygenase (IDO, including IDO1 and IDO2) and its inducers (including pro-inflammatory cytokines interferon [IFN]-γ, tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-2 and IL-6) were genotyped using SNPscan™ technology, and serum IDO1 levels were detected by double-antibody sandwich enzyme-linked immune-sorbent assay.ResultsFifty-nine patients (31.72%) were diagnosed with depression at 2 weeks after stroke onset (early-onset PSD). The IDO1 rs9657182 T/T genotype was independently associated with early-onset PSD (adjusted odds ratio [OR] = 3.008, 95% confidence interval [CI] 1.157-7.822, p = 0.024) and the frequency of rs9657182 T allele was significantly higher in patients with PSD than that in patients with non-PSD (χ2 = 4.355, p = 0.037), but these results did not reach the Bonferroni significance threshold (p > 0.003). Serum IDO1 levels were also independently linked to early-onset PSD (adjusted OR = 1.071, 95% CI 1.002-1.145, p = 0.044) and patients with PSD had higher serum IDO1 levels than patients with non-PSD in the presence of the rs9657182 T allele but not homozygous C allele (t = -2.046, p = 0.043). Stroke patients with the TNF-α rs361525 G/G genotype had higher serum IDO1 levels compared to those with the G/A genotype (Z = -2.451, p = 0.014).ConclusionsOur findings provided evidence that IDO1 gene polymorphisms and protein levels were involved in the development of early-onset PSD and TNF-α polymorphism was associated with IDO1 levels, supporting that IDO1 which underlie strongly regulation by cytokines may be a specific pathway for the involvement of immune-inflammatory mechanism in the pathophysiology of PSD

The value of diffusion kurtosis imaging in assessing mismatch repair gene expression of rectal carcinoma: Preliminary findings.

PURPOSE:To determine the correlation between the parameters of MR diffusion kurtosis imaging (MR-DKI) and mismatch repair gene expression (MMR) for rectal carcinomas. MATERIALS AND METHODS:Data from 80 patients with rectal carcinoma were analyzed in this prospective study. High-resolution T2WI and DKI (b = 0, 800 and 1600 s/mm2, respectively) were performed. Mean kurtosis (MK) and mean diffusivity (MD) from DKI were measured. MMR-positive expression and HER-2 expression were classified into two groups. For comparison between different grades, the Mann-Whitney U test, receiver operating characteristic curve, and Spearman's correlation analysis were used for statistical analyses. RESULTS:The MK values in identifying positive MMR expressions (MLH1, MSH2, and MSH6) were more reliable than the MD values (rs value: 0.772 vs. 0.448, 0.733 vs. 0.499, and 0.828 vs. 0.633 respectively, P0.05). Similarly, MK values were better than MD values in identifying HER2 expression (z = 2.795, P<0.05). CONCLUSIONS:MK derived from DKI demonstrated a greater correlation than MD with MMR expression. It also showed better performance in differentiating between high- and low-grade positive MMR expression and HER2 expression. Thus, DKI may be valuable for the prognoses and evaluation of non-invasive therapies

Genome-wide DNA polymorphisms in four Actinidia arguta genotypes based on whole-genome re-sequencing.

Among the genus Actinidia, Actinidia arguta possesses the strongest cold resistance and produces fresh fruit with an intense flavor. To investigate genomic variation that may contribute to variation in phenotypic traits, we performed whole-genome re-sequencing of four A. arguta genotypes originating from different regions in China and identified the polymorphisms using InDel markers. In total, 4,710,650, 4,787,750, 4,646,026, and 4,590,616 SNPs and 1,481,002, 1,534,198, 1,471,304, and 1,425,393 InDels were detected in the 'Ruby-3', 'Yongfeng male', 'Kuilv male', and 'Hongbei male' genomes, respectively, compared with the reference genome sequence of cv 'Hongyang'. A subset of 120 InDels were selected for re-sequencing validation. Additionally, genes related to non-synonymous SNPs and InDels in coding domain sequences were screened for functional analysis. The analysis of GO and KEGG showed that genes involved in cellular responses to water deprivation, sucrose transport, decreased oxygen levels and plant hormone signal transduction were significantly enriched in A. arguta. The results of this study provide insight into the genomic variation of kiwifruit and can inform future research on molecular breeding to improve cold resistance in kiwifruit

Table_1_Association of homocysteine and polymorphism of methylenetetrahydrofolate reductase with early-onset post stroke depression.DOCX

BackgroundHomocysteine (Hcy) has been indicated to be involved in pathophysiology of post stroke depression (PSD). There is a lack of research to study the relationship between Hcy metabolism genes and PSD. Our study aims to investigate the relationship among Hcy metabolism genes, Hcy, and early-onset PSD.Materials and methodsWe recruited 212 patients with stroke and collected their peripheral blood sample, clinical data, and laboratory test on admission. 12 single nucleotide polymorphisms (SNPs) in methylenetetrahydrofolate reductase (MTHFR), methionine synthase reductase (MTRR), and methionine synthase (MTR) genes were genotyped by high-resolution melt analysis. PSD was diagnosed by DSM-V at 2 weeks after stroke. Binary logistic regression and haplotype analysis were used to examine the association between Hcy metabolism genes and PSD. Mediation analysis was performed to clarify whether the SNPs exerted their effect on PSD by affecting the Hcy level.Results81 patients were diagnosed with PSD, and the incidence rate was 38.2%. Hcy level in PSD group was significantly higher than it in non-PSD group (p = 0.019). MTHFR rs1801133 AA genotype an A allele were associated with an elevated risk of PSD after adjustment for some confounding factors (OR = 4.021, 95% CI: 1.459∼11.080, p = 0.007 for AA genotype; OR = 1.808, 95% CI: 1.172∼2.788, p = 0.007 for A allele). Furthermore, the effect of MTHFR rs1801133 AA genotype on PSD was mediated by Hcy (OR = 1.569, 95% CI: 0.013∼3.350, p ConclusionMTHFR rs1801133 and Hcy were associated with PSD, and MTHFR rs1801133 may exert an effect on PSD via mediating Hcy level. This offers a new perspective for treating PSD and understanding the mechanism of PSD.</p