53 research outputs found
A novel protein-coding ORF72.2 gene was identified from Marek's disease virus strain CVI988
Marek's disease is a highly contagious disease of poultry characterized by rapid-on set of T-cell lymphomas, which is caused by Marek's disease virus (MDV), but its pathogenic mechanism is still not very clear. Recently, some new progress were achieved in molecular character of MDV. Along with the genomic sequencing of MDV serotype 1, some novel open reading frames (ORFs) were predicted, and ORF72.2 was one of them which have no homologues in other MDV serotypes or in other alphaherpesvirus. In the study, ORF72.2 was firstly identified as a protein-coding gene by the method of reverse transcription polymerase chain reaction (RT-PCR), western blotting and indirect immunofluorescence assay. This study paved the way to conduct further studies to determine whether ORF72.2 plays a role in MDV replication and pathogenicity
Cubic phase nanoparticles for sustained release of ibuprofen formulation characterization and enhanced bioavailability study
Normative Analysis of Individual Brain Differences Based on a Population MRI-Based Atlas of Cynomolgus Macaques
The developmental trajectory of the primate brain varies substantially with aging across subjects. However, this ubiquitous variability between individuals in brain structure is difficult to quantify and has thus essentially been ignored. Based on a large-scale structural magnetic resonance imaging dataset acquired from 162 cynomolgus macaques, we create a species-specific 3D template atlas of the macaque brain, and deploy normative modeling to characterize individual variations of cortical thickness (CT) and regional gray matter volume (GMV). We observed an overall decrease in total GMV and mean CT, and an increase in white matter volume from juvenile to early adult. Specifically, CT and regional GMV were greater in prefrontal and temporal cortices relative to early unimodal areas. Age-dependent trajectories of thickness and volume for each cortical region revealed an increase in the medial temporal lobe, and decreases in all other regions. A low percentage of highly individualized deviations of CT and GMV were identified (0.0021%, 0.0043%, respectively, P \u3c 0.05, false discovery rate [FDR]-corrected). Our approach provides a natural framework to parse individual neuroanatomical differences for use as a reference standard in macaque brain research, potentially enabling inferences regarding the degree to which behavioral or symptomatic variables map onto brain structure in future disease studies
The Adaptive Gain Integrating Pixel Detector at the European XFEL
The Adaptive Gain Integrating Pixel Detector (AGIPD) is an x-ray imager,
custom designed for the European x-ray Free-Electron Laser (XFEL). It is a
fast, low noise integrating detector, with an adaptive gain amplifier per
pixel. This has an equivalent noise of less than 1 keV when detecting single
photons and, when switched into another gain state, a dynamic range of more
than 10 photons of 12 keV. In burst mode the system is able to store 352
images while running at up to 6.5 MHz, which is compatible with the 4.5 MHz
frame rate at the European XFEL. The AGIPD system was installed and
commissioned in August 2017, and successfully used for the first experiments at
the Single Particles, Clusters and Biomolecules (SPB) experimental station at
the European XFEL since September 2017. This paper describes the principal
components and performance parameters of the system.Comment: revised version after peer revie
Gene editing in monogenic autism spectrum disorder: animal models and gene therapies
Autism spectrum disorder (ASD) is a lifelong neurodevelopmental disease, and its diagnosis is dependent on behavioral manifestation, such as impaired reciprocal social interactions, stereotyped repetitive behaviors, as well as restricted interests. However, ASD etiology has eluded researchers to date. In the past decades, based on strong genetic evidence including mutations in a single gene, gene editing technology has become an essential tool for exploring the pathogenetic mechanisms of ASD via constructing genetically modified animal models which validates the casual relationship between genetic risk factors and the development of ASD, thus contributing to developing ideal candidates for gene therapies. The present review discusses the progress in gene editing techniques and genetic research, animal models established by gene editing, as well as gene therapies in ASD. Future research should focus on improving the validity of animal models, and reliable DNA diagnostics and accurate prediction of the functional effects of the mutation will likely be equally crucial for the safe application of gene therapies
Megapixels @ Megahertz -- The AGIPD High-Speed Cameras for the European XFEL
The European XFEL is an extremely brilliant Free Electron Laser Source with a
very demanding pulse structure: trains of 2700 X-Ray pulses are repeated at 10
Hz. The pulses inside the train are spaced by 220 ns and each one contains up
to photons of 12.4 keV, while being fs in length. AGIPD,
the Adaptive Gain Integrating Pixel Detector, is a hybrid pixel detector
developed by DESY, PSI, and the Universities of Bonn and Hamburg to cope with
these properties. It is a fast, low noise integrating detector, with single
photon sensitivity (for keV) and a large dynamic
range, up to photons at 12.4 keV. This is achieved with a charge
sensitive amplifier with 3 adaptively selected gains per pixel. 352 images can
be recorded at up to 6.5 MHz and stored in the in-pixel analogue memory and
read out between pulse trains. The core component of this detector is the AGIPD
ASIC, which consists of pixels of . Control of the ASIC's image acquisition and analogue readout is
via a command based interface. FPGA based electronic boards, controlling ASIC
operation, image digitisation and 10 GE data transmission interface AGIPD
detectors to DAQ and control systems. An AGIPD 1 Mpixel detector has been
installed at the SPB experimental station in August 2017, while a second one is
currently commissioned for the MID endstation. A larger (4 Mpixel) AGIPD
detector and one to employ Hi-Z sensor material to efficiently register photons
up to keV are currently under construction.Comment: submitted to the proceedings of the ULITIMA 2018 conference, to be
published in NIM
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