Study of zeolite influence on analytical characteristics of urea biosensor based on ion-selective field-effect transistors

A possibility of the creation of potentiometric biosensor by adsorption of enzyme urease on zeolite was investigated. Several variants of zeolites (nano beta, calcinated nano beta, silicalite, and nano L) were chosen for experiments. The surface of pH-sensitive field-effect transistors was modified with particles of zeolites, and then the enzyme was adsorbed. As a control, we used the method of enzyme immobilization in glutaraldehyde vapour (without zeolites). It was shown that all used zeolites can serve as adsorbents (with different effectiveness). The biosensors obtained by urease adsorption on zeolites were characterized by good analytical parameters (signal reproducibility, linear range, detection limit and the minimal drift factor of a baseline). In this work, it was shown that modification of the surface of pH-sensitive field-effect transistors with zeolites can improve some characteristics of biosensors

ВОЗМОЖНОСТИ ЖИДКОСТНОЙ ЦИТОЛОГИИ В ДИАГНОСТИКЕ РАКА МОЧЕВОГО ПУЗЫРЯ

The study included 323 patients (the first group - 150 patients with suspected bladder cancer, the second - 173 patients with bladder cancer after treatment). All patients underwent cytological investigation of washings of bladder using conventional method and liquid cytology. This study presents comparative characteristics of these methods and shows considerable effectiveness of the method of liquid cytology in the diagnosis of bladder cancer and its local recurrence.В исследование  включено 323 человека (первая  группа – 150 пациентов с подозрением на рак мочевого пузыря, вторая – 173 больных раком мочевого пузыря, после проведенного лечения). Всем обследуемым было выполнено цитологическое исследование  промывных вод мочевого пузыря традиционным методом и методом жидкостной цитологии. Дана сравнительная характеристика этих методов исследования и показана значительная эффективность метода жидкостной цитологии в диагностике рака мочевого пузыря и его местных рецидивов

Совершенствование цитологической диагностики рака мочевого пузыря

The paper gives the comparative results of cytological examination of alcohol-induced bladder washouts by liquid-based cytology and conventional cytology in 323 patients, including 150 with suspected bladder cancer (BC) and 173 patients after performed combination or combined treatment for BC. The performed investigation has established that the diagnostic value of liquid-based cytology in diagnosing BC and its local recurrences is 1.3-fold higher than that of conventional cytology.В работе представлены сравнительные результаты цитологического исследования методом жидкостной цитологии и традиционного цитологического исследования спиртовых смывов мочевого пузыря 323 пациентов, 150 из которых были с подозрением на рак мочевого пузыря (РМП) и 173 пациента – после проведенного комплексного или комбинированного лечения по поводу РМП. В результате проведенного исследования установлено, что диагностическая ценность метода жидкостной цитологии в диагностике РМП и его местных рецидивов в 1,3 раза выше традиционного цитологического метода

FAST-TRACK HIP AND KNEE ARTHROPLASTY (LITERATURE REVIEW)

In the present paper the authors discuss the history of fast-track formation, its development and current status. Fast-track advantages in comparison to other rehabilitation options are analyzed. The focus is placed on methods of accelerated rehabilitation after total hip and knee arthroplasty in the leading European and American clinics. Fast-track protocols of surgical centers of Denmark and Hospital for special surgery in the US are described, factors that determine time of hospital stay after total hip and knee arthroplasty are considered, predictors of hospital stay and patients satisfaction after the surgery are examined. The paper reflects the results of the comparison of frequency of thromboembolic and other complications as well as readmission rate after traditional rehabilitation and after fast-track. The paper considers fast-track in bilateral total knee replacement. The authors conclude that further research on acceleration of rehabilitation and reduction of hospital stay after total hip and knee arthroplasty needs to be focused on the optimization of pain relief, especially after discharge, blood preservation techniques, supplementation of fluids loss and strengthening of muscles. Literature analysis demonstrated that the implementation of the Protocol for accelerated recovery after joint arthroplasty in Russia is at an insufficient level. Data of foreign multicenter studies during recent years indicate a high efficacy and safety of this technique in different groups of patients

FAST-TRACK HIP AND KNEE ARTHROPLASTY (LITERATURE REVIEW)

In the present paper the authors discuss the history of fast-track formation, its development and current status. Fast-track advantages in comparison to other rehabilitation options are analyzed. The focus is placed on methods of accelerated rehabilitation after total hip and knee arthroplasty in the leading European and American clinics. Fast-track protocols of surgical centers of Denmark and Hospital for special surgery in the US are described, factors that determine time of hospital stay after total hip and knee arthroplasty are considered, predictors of hospital stay and patients satisfaction after the surgery are examined. The paper reflects the results of the comparison of frequency of thromboembolic and other complications as well as readmission rate after traditional rehabilitation and after fast-track. The paper considers fast-track in bilateral total knee replacement. The authors conclude that further research on acceleration of rehabilitation and reduction of hospital stay after total hip and knee arthroplasty needs to be focused on the optimization of pain relief, especially after discharge, blood preservation techniques, supplementation of fluids loss and strengthening of muscles. Literature analysis demonstrated that the implementation of the Protocol for accelerated recovery after joint arthroplasty in Russia is at an insufficient level. Data of foreign multicenter studies during recent years indicate a high efficacy and safety of this technique in different groups of patients

The increased risk zones of bladder cancer emergence

The prevalence of incidence and mortality from a bladder cancer in Krasnodar region during 2003-2012 was studied in this article on the basis of documents. The analysis of spatial distribution of incidence and mortality frequency from a bladder cancer on 44 municipalities of Krasnodar region is carried out by means of mapping and risk zones of bladder cancer cases are allocated. Recommendations about preventive actions carrying out in these zones are presented here

Improvement of the cytological diagnosis of bladder cancer

<p>The paper gives the comparative results of cytological examination of alcohol-induced bladder washouts by liquid-based cytology and conventional cytology in 323 patients, including 150 with suspected bladder cancer (BC) and 173 patients after performed combination or combined treatment for BC. The performed investigation has established that the diagnostic value of liquid-based cytology in diagnosing BC and its local recurrences is 1.3-fold higher than that of conventional cytology.</p

Application of different zeolites for improvement of the characteristics of a pH-FET biosensor based on immobilized urease

International audienceDifferent modifications of the zeolites Na+-Beta and LTA were applied for improving the working characteristics of a urea biosensor. The bioselective membrane of the biosensor was based on urease and different zeolites co-immobilized with bovine serum albumin on the surface of a pH-FET. It was shown that the biosensors modified with the zeolites H+-Beta30 and H+-Beta50 are characterized by increased sensitivity to urea. The influence of the zeolite concentration on the sensitivity of the biosensors was studied. The optimal concentration of the zeolites H+-Beta30 and H+-Beta50 in the bioselective membrane was 15%. Different variants of co-immobilization of urease and zeolite H+-Beta30 were studied and the optimal method was selected. Thus, a general conclusion is that the urea biosensor sensitivity can be improved using zeolite H+-Beta30 for urease immobilization in the bioselective membrane