Study of zeolite influence on analytical characteristics of urea biosensor based on ion-selective field-effect transistors

A possibility of the creation of potentiometric biosensor by adsorption of enzyme urease on zeolite was investigated. Several variants of zeolites (nano beta, calcinated nano beta, silicalite, and nano L) were chosen for experiments. The surface of pH-sensitive field-effect transistors was modified with particles of zeolites, and then the enzyme was adsorbed. As a control, we used the method of enzyme immobilization in glutaraldehyde vapour (without zeolites). It was shown that all used zeolites can serve as adsorbents (with different effectiveness). The biosensors obtained by urease adsorption on zeolites were characterized by good analytical parameters (signal reproducibility, linear range, detection limit and the minimal drift factor of a baseline). In this work, it was shown that modification of the surface of pH-sensitive field-effect transistors with zeolites can improve some characteristics of biosensors

Совершенствование цитологической диагностики рака мочевого пузыря

The paper gives the comparative results of cytological examination of alcohol-induced bladder washouts by liquid-based cytology and conventional cytology in 323 patients, including 150 with suspected bladder cancer (BC) and 173 patients after performed combination or combined treatment for BC. The performed investigation has established that the diagnostic value of liquid-based cytology in diagnosing BC and its local recurrences is 1.3-fold higher than that of conventional cytology.В работе представлены сравнительные результаты цитологического исследования методом жидкостной цитологии и традиционного цитологического исследования спиртовых смывов мочевого пузыря 323 пациентов, 150 из которых были с подозрением на рак мочевого пузыря (РМП) и 173 пациента – после проведенного комплексного или комбинированного лечения по поводу РМП. В результате проведенного исследования установлено, что диагностическая ценность метода жидкостной цитологии в диагностике РМП и его местных рецидивов в 1,3 раза выше традиционного цитологического метода

ВОЗМОЖНОСТИ ЖИДКОСТНОЙ ЦИТОЛОГИИ В ДИАГНОСТИКЕ РАКА МОЧЕВОГО ПУЗЫРЯ

The study included 323 patients (the first group - 150 patients with suspected bladder cancer, the second - 173 patients with bladder cancer after treatment). All patients underwent cytological investigation of washings of bladder using conventional method and liquid cytology. This study presents comparative characteristics of these methods and shows considerable effectiveness of the method of liquid cytology in the diagnosis of bladder cancer and its local recurrence.В исследование  включено 323 человека (первая  группа – 150 пациентов с подозрением на рак мочевого пузыря, вторая – 173 больных раком мочевого пузыря, после проведенного лечения). Всем обследуемым было выполнено цитологическое исследование  промывных вод мочевого пузыря традиционным методом и методом жидкостной цитологии. Дана сравнительная характеристика этих методов исследования и показана значительная эффективность метода жидкостной цитологии в диагностике рака мочевого пузыря и его местных рецидивов

SIMULATION AND DEVELOPMENT OF MASS-DETERMINES WITH AIR INTERLAYER FOR CONTROL SYSTEM OF PRODUCTION OF UNCURED TYRE TREAD MATERIALS

There the mathematical simulator of flow of air layer with the changing thickness in the pneumomechanic mass-determing devices has been obtained. The structural scheme of mass-determiners of pneumoconveyers have been developed; the choice technique of structural materials, of variables and of operating characteristics of pneumomechanic mass-determing section has been offered. The introduction subject is the pneumoconveyer with with the mass-determing section. The efficiency is the improvement of convection properties of product at the expense of decrease of number of mechanic contacts.The application field is the production of polymeric materials, of rubber-engineering articles etcAvailable from VNTIC / VNTIC - Scientific & Technical Information Centre of RussiaSIGLERURussian Federatio

FAST-TRACK HIP AND KNEE ARTHROPLASTY (LITERATURE REVIEW)

In the present paper the authors discuss the history of fast-track formation, its development and current status. Fast-track advantages in comparison to other rehabilitation options are analyzed. The focus is placed on methods of accelerated rehabilitation after total hip and knee arthroplasty in the leading European and American clinics. Fast-track protocols of surgical centers of Denmark and Hospital for special surgery in the US are described, factors that determine time of hospital stay after total hip and knee arthroplasty are considered, predictors of hospital stay and patients satisfaction after the surgery are examined. The paper reflects the results of the comparison of frequency of thromboembolic and other complications as well as readmission rate after traditional rehabilitation and after fast-track. The paper considers fast-track in bilateral total knee replacement. The authors conclude that further research on acceleration of rehabilitation and reduction of hospital stay after total hip and knee arthroplasty needs to be focused on the optimization of pain relief, especially after discharge, blood preservation techniques, supplementation of fluids loss and strengthening of muscles. Literature analysis demonstrated that the implementation of the Protocol for accelerated recovery after joint arthroplasty in Russia is at an insufficient level. Data of foreign multicenter studies during recent years indicate a high efficacy and safety of this technique in different groups of patients

The increased risk zones of bladder cancer emergence

The prevalence of incidence and mortality from a bladder cancer in Krasnodar region during 2003-2012 was studied in this article on the basis of documents. The analysis of spatial distribution of incidence and mortality frequency from a bladder cancer on 44 municipalities of Krasnodar region is carried out by means of mapping and risk zones of bladder cancer cases are allocated. Recommendations about preventive actions carrying out in these zones are presented here

FAST-TRACK HIP AND KNEE ARTHROPLASTY (LITERATURE REVIEW)

In the present paper the authors discuss the history of fast-track formation, its development and current status. Fast-track advantages in comparison to other rehabilitation options are analyzed. The focus is placed on methods of accelerated rehabilitation after total hip and knee arthroplasty in the leading European and American clinics. Fast-track protocols of surgical centers of Denmark and Hospital for special surgery in the US are described, factors that determine time of hospital stay after total hip and knee arthroplasty are considered, predictors of hospital stay and patients satisfaction after the surgery are examined. The paper reflects the results of the comparison of frequency of thromboembolic and other complications as well as readmission rate after traditional rehabilitation and after fast-track. The paper considers fast-track in bilateral total knee replacement. The authors conclude that further research on acceleration of rehabilitation and reduction of hospital stay after total hip and knee arthroplasty needs to be focused on the optimization of pain relief, especially after discharge, blood preservation techniques, supplementation of fluids loss and strengthening of muscles. Literature analysis demonstrated that the implementation of the Protocol for accelerated recovery after joint arthroplasty in Russia is at an insufficient level. Data of foreign multicenter studies during recent years indicate a high efficacy and safety of this technique in different groups of patients

The epidemiology of the primary brain tumors in Rostov region in 2010-2015

The dynamics of the prevalence, incidence, and clinical and statistical characteristics of primary and metastatic brain tumors (BT) with regard to their histological types in the Rostov region, depending on the environmental conditions of life, different age and gender and social groups who were treated in specialized and non-specialized hospitals of Rostov-on-Don for the period from 1 january 2010 to 2015 is a proven result. A further increase in the level of incidence of malignant brain tumors, both due to the improved diagnostics of tumor diseases of the brain, and the influence of climatic, demographic, environmental and medico-social characteristics of the study region were showed. However, many epidemiological aspects of neoplastic diseases of the brain remains poorly understood, requires the continuation of this study

Investigation of characteristics of urea and butyrylcholine chloride biosensors based on ion-selective field-effect transistors modified by the incorporation of heat-treated zeolite Beta crystals

Urea and butyrylcholine chloride (BuChCl) biosensors were prepared by adsorption of urease and butyrylcholinesterase (BuChE) on heat-treated zeolite Beta crystals, which were incorporated into membranes deposited on ion-selective field-effect transistor (ISFET) surfaces. The responses, stabilities, and use for inhibition analysis of these biosensors were investigated. Different heat treatment procedures changed the amount of Bronsted acid sites without affecting the size, morphology, overall Si/Al ratio, external specific surface area, and the amount of terminal silanol groups in zeolite crystals. Upon zeolite incorporation the enzymatic responses of biosensors towards urea and BuChCl increased up to similar to 2 and similar to 5 times, respectively; and correlated with the amount of Bronsted acid sites. All biosensors demonstrated high signal reproducibility and stability for both urease and BuChE. The inhibition characteristics of urease and BuChE were also related to the Bronsted acidity. The pore volume and pore size increases measured for the heat-treated samples are very unlikely causes for the improvements observed in biosensors' performance, because urease and BuChE are approximately one order of magnitude larger than the resulting zeolite Beta pores. Overall, these results suggest that the zeolites incorporated into the biologically active membrane with enhanced Bronsted acidity can improve the performance of ISFET-based biosensors