Constraining Primordial Magnetism

Primordial magnetic fields could provide an explanation for the galactic magnetic fields observed today, in which case they may also leave interesting signals in the CMB and the small-scale matter power spectrum. We discuss how to approximately calculate the important non-linear magnetic effects within the guise of linear perturbation theory, and calculate the matter and CMB power spectra including the SZ contribution. We then use various cosmological datasets to constrain the form of the magnetic field power spectrum. Using solely large-scale CMB data (WMAP7, QUaD and ACBAR) we find a 95% CL on the variance of the magnetic field at 1 Mpc of B_\lambda < 6.4 nG. When we include SPT data to constrain the SZ effect, we find a revised limit of B_\lambda < 4.1 nG. The addition of SDSS Lyman-alpha data lowers this limit even further, roughly constraining the magnetic field to B_\lambda < 1.3 nG.Comment: 12 pages, 9 figure

Silent ischemia: Evaluation by exercise and redistribution tomographic thallium-201 myocardial imaging

AbstractTo compare the amount of myocardium jeopardized during silent ischemia and painful ischemia, 112 consecutive patients undergoing coronary arteriography with ischemia demonstrated by exercise and redistribution tomographic thallium-201 myocardial imaging (SPECT) were divided into two groups: 84 patients without anginal pain (silent ischemia) and 28 with pain (painful ischemia). The SPECT apical, mid and basal ventricular levels of the short-axis view and the apical portion of the long-axis view were divided into 20 segments.The results were 1) 7.4 ± 4.7 ischemic segments in silent ischemia and 7.6 ± 3.7 in painful ischemia (p = NS) with 4.7 ± 3.6 segments in silent ischemia undergoing total redistribution compared with 5.4 ± 3.4 in painful ischemia (p = NS); 2) no difference in the incidence of single, double or triple vessel disease between silent and painful ischemic groups; 3) similar anatomic distribution of ischemic segments between the two groups; 4) more positive exercise electrocardiographic (ECG) changes in painful ischemia (70%) than in silent ischemia (32%) (p < 0.001) with equal amounts of ischemia associated with positive and negative exercise ECG findings.Conclusions: 1) Patients with silent and painful ischemia during exercise have similar amounts of ischemic myocardium demonstrated by tomographic thallium-201 imaging and similar extent of angiographically documented coronary artery disease despite the absence of pain and the lower incidence of positive exercise ECG findings in silent ischemia. 2) Positive and negative exercise ECG findings were associated with similar amounts of ischemic myocardium

Methylation enrichment pyrosequencing: combining the specificity of MSP with validation by pyrosequencing

It has been suggested that detection of aberrant DNA methylation in clinical specimens such as sputum or saliva may be a valuable tumour biomarker. Any clinically applicable detection technique must combine high sensitivity with high specificity. In this study we describe methylation enrichment pyrosequencing (MEP), which benefits from the high sensitivity and specificity of methylation-specific PCR (MSP) but has a second, confirmatory, pyrosequencing step. The pyrosequencing reaction is rapid, relatively inexpensive and offers significant logistical advantages over previously described validation methods. As proof of principle, we illustrate MEP using assays of p16 and cyclin A1 promoters in a methylated DNA dilution matrix and also in a clinical setting using paired saliva and oral tumour specimens. Our results confirm that mis-priming of MSP, with subsequent false positive results, can occur frequently (perhaps 10%) in assays combining high numbers of PCR cycles and low concentrations of starting DNA. In our clinical example, MEP of saliva-derived DNA was more sensitive than standard non-methylation-specific pyrosequencing as illustrated using p16 and cyclin A1 promoter methylation assays

The direct synthesis of hydrogen peroxide from H₂ and O₂ using Pd-Ni/TiO₂ catalysts

The direct synthesis of hydrogen peroxide (H2O2) from molecular H2 and O2 offers an attractive solution to decentralised production compared to the anthraquinone process. Herein we evaluate the performance of a 0.5%Pd-4.5%Ni/TiO2 catalyst in batch and flow reactor systems using water as a solvent at ambient temperature which makes synthesising high H2O2 concentrations challenging. Catalyst activity was observed to be stable to prolonged use in multiple batch experiments or in a flow system, with selectivities towards H2O2 of 97% and 85% respectively. This study was carried out in the absence of halide or acid additives that are typically used to inhibit sequential H2O2 degradation reactions showing that this Pd-Ni catalyst has potential to produce H2O2 selectivel

CpG island methylation phenotype (CIMP) in oral cancer: associated with a marked inflammatory response and less aggressive tumour biology.

Studies in several tumour sites highlight the significance of the CpG island methylation phenotype (CIMP), with distinct features of histology, biological aggression and outcome. We utilise pyrosequencing techniques of quantitative methylation analysis to investigate the presence of CIMP in oral squamous cell carcinoma (OSCC) for the first time, and evaluate its correlation with allelic imbalance, pathology and clinical behaviour. Tumour tissue, control tissue and PBLs were obtained from 74 patients with oral squamous cell carcinoma. Pyrosequencing was used to analyse methylation patterns in 75-200 bp regions of the CpG rich gene promoters of 10 genes with a broad range of cellular functions. Allelic imbalance was investigated using a multiplexed panel of 11 microsatellite markers. Corresponding variables, histopathological staging and grading were correlated with these genetic and epigenetic aberrations. A cluster of tumours with a greater degree of promoter methylation than would be predicted by chance alone (P=0.001) were designated CIMP+ve. This group had less aggressive tumour biology in terms of tumour thickness (p=0.015) and nodal metastasis (P=0.012), this being apparently independent of tumour diameter. Further, it seems that these CIMP+ve tumours excited a greater host inflammatory response (P=0.019). The exact mechanisms underlying CIMP remain obscure but the association with a greater inflammatory host response supports existing theories relating these features in other tumour sites. As CIMP has significant associations with other well documented prognostic indicators, it may prove beneficial to include methylation analyses in molecular risk modelling of tumours

Are there gender differences in the geography of alcohol-related mortality in Scotland? An ecological study

&lt;b&gt;Background&lt;/b&gt; There is growing concern about alcohol-related harm, particularly within Scotland which has some of the highest rates of alcohol-related death in western Europe. There are large gender differences in alcohol-related mortality rates in Scotland and in other countries, but the reasons for these differences are not clearly understood. In this paper, we aimed to address calls in the literature for further research on gender differences in the causes, contexts and consequences of alcohol-related harm. Our primary research question was whether the kind of social environment which tends to produce higher or lower rates of alcohol-related mortality is the same for both men and women across Scotland. &lt;b&gt;Methods&lt;/b&gt; Cross-sectional, ecological design. A comparison was made between spatial variation in men's and women's age-standardised alcohol-related mortality rates in Scotland using maps, Moran's Index, linear regression and spatial analyses of residuals. Directly standardised mortality rates were derived from individual level records of death registration, 2000–2005 (n = 8685). &lt;b&gt;Results&lt;/b&gt; As expected, men's alcohol-related mortality rate substantially exceeded women's and there was substantial spatial variation in these rates for both men and women within Scotland. However, there was little spatial variation in the relationship between men's and women's alcohol-mortality rates (r2 = 0.73); areas with relatively high rates of alcohol-related mortality for men tended also to have relatively high rates for women. In a small number of areas (8 out of 144) the relationship between men's and women's alcohol-related mortality rates was significantly different. &lt;b&gt;Conclusion&lt;/b&gt; In as far as geographic location captures exposure to social and economic environment, our results suggest that the relationship between social and economic environment and alcohol-related harm is very similar for men and women. The existence of a small number of areas in which men's and women's alcohol-related mortality had an different relationship suggests that some places may have unusual drinking cultures. These might prove useful for further investigations into the factors which influence drinking behaviour in men and women

Restenosis after excellent angiographic angioplasty result for chronic total coronary artery occlusion--Implications for newer percutaneous revascularization devices

The incidence of restenosis after coronary angioplasty for treatment of chronic total coronary occlusion is unacceptably high.1 The pathophysiology of restenosis after coronary angioplasty may be conceptually divided into an exuberant myointimal proliferation,2 and a residual partial obstruction that serves as a platform for atheroma regrowth and may potentiate that process by augmenting blood flow turbulence and platelet deposition. The techniques of atherectomy or laser ablation3 may lessen the likelihood of restenosis by minimizing the residual stenosis, although currently each may require supplemental balloon angioplasty to achieve this result. However, the effect of these techniques on later myointimal proliferation in human beings is largely unknown. The concept that intracoronary stenting4 may reduce restenosis is based largely on the supposition that, by forcing and maintaining the obstructive atheroma out of the normal arterial lumen, turbulence and hence platelet deposition would be reduced5 and a large amount of myointimal proliferation would be required to recreate an obstruction of physiologic consequence.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/27757/1/0000150.pd

Contrasting longitudinal and cross-sectional relationships between insulin resistance and percentage of body fat, fitness, and physical activity in children - the LOOK study

Telford RD, Cunningham RB, Shaw JE, Dunstan DW, Lafferty ARA, Reynolds GJ, Hickman PE, Southcott E, Potter JM, Waring P, Telford RM. Contrasting longitudinal and cross-sectional relationships between insulin resistance and percentage of body fat, fitnes