The innervation of the enthesis organ of the rat achilles tendon

PURPOSE: The enthesis (bony insertion of a tendon or ligament) is a common site of overuse injuries in sport. Because enthesopathies can be painful, it is often assumed that the enthesis is highly innervated-but with little evidence to support the assumption. The Achilles tendon has one of the most complex of attachment sites, for together with adjacent tissues, the enthesis itself forms part of an 'enthesis organ' which reduces stress concentration at the bony interface. These adjacent structures include a 'sesamoid fibrocartilage' in the tendon, a 'periosteal fibrocartilage' on the superior tuberosity of the calcaneus, and a fat pad which extends into the retrocalcaneal bursa during plantarflexion. The purpose of the present study is to investigate the innervation of the whole enthesis organ complex. METHODS: The tendon attachment site was removed from one leg of 3 male Wistar rats at each of the following ages-neonates, 4 weeks, 12 weeks, and 24 months. The tissue was fixed in 4% paraformaldehyde, prepared for routine indirect immunohistochemistry and cryosectioned in the sagittal plane. Serial sections were immunolabelled with polyclonal antibodies to protein gene product 9.5, substance P, calcitonin gene related peptide and neurofilament 200. Histology reference sections were stained with toluidine blue. RESULTS: No nerve fibers were detected at the enthesis itself or in the sesamoid and periosteal fib ro cartilages in rats of any age. However, the fat pad was richly supplied by nerve fibers which immunolabelled with all of the antibodies used. It also contained abundant mast cells. The innervation of the fat pad was confirmed in 10 human Achilles tendons obtained from the Department of Forensic Medicine at the Ludwig-Maximilians-Universität, in accordance with the ethical regulations of Munich University. The nerve fibers again immunolabelled with all antibodies and formed an intricate network in which the fibers lay between individual fat cells. CONCLUSION: We suggest that healthy entheses are not innervated because of the high levels of mechanical loading experienced at insertion sites. However, the striking innervation of the adjacent fat pad suggests that it may have an unheralded proprioceptive role monitoring changes in insertional angle between tendon and bone that occur as a result of foot movements

Multi-User Droplet Combustion Apparatus - Flame Extinguishment Experiment

Multi-User Droplet Combustion Apparatus Flame Extinguishment Experiment (MDCA-FLEX) will assess the effectiveness of fire suppressants in microgravity and quantify the effect of different possible crew exploration atmospheres on fire suppression. The goal of this research is to provide definition and direction for large scale fire suppression tests and selection of the fire suppressant for next generation crew exploration vehicles

Fiber Supported Droplet Combustion-2 (FSDC-2)

Experimental results for the burning characteristics of fiber supported, liquid droplets in ambient Shuttle cabin air (21% oxygen, 1 bar pressure) were obtained from the Glove Box Facility aboard the STS-94/MSL-1 mission using the Fiber Supported Droplet Combustion - 2 (FSDC-2) apparatus. The combustion of individual droplets of methanol/water mixtures, ethanol, ethanol/water azeotrope, n-heptane, n-decane, and n-heptane/n-hexadecane mixtures were studied in quiescent air. The effects of low velocity, laminar gas phase forced convection on the combustion of individual droplets of n-heptane and n-decane were investigated and interactions of two droplet-arrays of n-heptane and n-decane droplets were also studied with and without gas phase convective flow. Initial diameters ranging from about 2mm to over 6mm were burned on 80-100 micron silicon fibers. In addition to phenomenological observations, quantitative data were obtained in the form of backlit images of the burning droplets, overall flame images, and radiometric combustion emission measurements as a function of the burning time in each experiment. In all, 124 of the 129 attempted experiments (or about twice the number of experiments originally planned for the STS-94/MSL-1 mission) were conducted successfully. The experimental results contribute new observations on the combustion properties of pure alkanes, binary alkane mixtures, and simple alcohols for droplet sizes not studied previously, including measurements on individual droplets and two-droplet arrays, inclusive of the effects of forced gas phase convection. New phenomena characterized experimentally for the first time include radiative extinction of droplet burning for alkanes and the "twin effect" which occurs as a result of interactions during the combustion of two-droplet arrays. Numerical modeling of isolated droplet combustion phenomenon has been conducted for methanol/water mixtures, n-heptane, and n-heptane/n-hexadecane mixtures, and results compare quantitatively with those found experimentally for methanol/water mixtures. Initial computational results qualitatively predict experimental results obtained for isolated n-heptane and n-heptane/n-hexadecane droplet combustion, although the effects of sooting are not yet included in the modeling work. Numerical modeling of ethanol and ethanol/water droplet burning is under development. Considerable data remain to be fully analyzed and will provide a large database for comparisons with further numerical and analytical modeling and development of future free droplet experiments aboard space platforms

A new Holocene sea-level record for Singapore

Relative sea-level (RSL) records from far-field regions distal from ice sheets remain poorly understood, particularly in the early Holocene. Here, we extended the Holocene RSL data from Singapore by producing early Holocene sea-level index points (SLIPs) and limiting dates from a new ~40 m sediment core. We merged new and published RSL data to construct a standardized Singapore RSL database consisting of 88 SLIPs and limiting data. In the early Holocene, RSL rose rapidly from −21.0 to −0.7 m from ~9500 to 7000 cal. yrs. BP. Thereafter, the rate of RSL rise decelerated, reaching a mid-Holocene highstand of 4.0 ± 4.5 m at 5100 cal. yrs. BP, before falling to its present level. There is no evidence of any inflections in RSL when the full uncertainty of SLIPs is considered. When combined with other standardized data from the Malay-Thai Peninsula, our results also show substantial misfits between regional RSL reconstructions and glacial isostatic adjustment (GIA) model predictions in the rate of early Holocene RSL rise, the timing of the mid-Holocene highstand and the nature of late-Holocene RSL fall towards the present. It is presently unknown whether these misfits are caused by regional processes, such as subsidence of the continental shelf, or inaccurate parameters used in the GIA model

Incorporating temporal and spatial variability of salt-marsh foraminifera into sea-level reconstructions

Foraminifera from salt-marsh environments have been used extensively in quantitative relative sea-level reconstructions due to their strong relationship with tidal level. However, the influence of temporal and spatial variability of salt-marsh foraminifera on quantitative reconstructions remains unconstrained. Here, we conducted a monitoring study of foraminifera from four intertidal monitoring stations in New Jersey from high marsh environments over three years that included several extreme weather (temperature, precipitation, and storm surge) events. We sampled four replicates from each station seasonally (four times per year) for a total of 188 samples. The dead foraminiferal assemblages were separated into four site-specific assemblages. After accounting for systematic trends in changes in foraminifera over time among stations, the distribution of foraminiferal assemblages across monitoring stations explained ~87% of the remaining variation, while ~13% can be explained by temporal and/or spatial variability among the replicate samples. We applied a Bayesian transfer function to estimate the elevation of the four monitoring stations. All samples from each station predicted an elevation estimate within a 95% uncertainty interval consistent with the observed elevation of that station. Combining samples into replicate- and seasonal-aggregate datasets decreased elevation estimate uncertainty, with the greatest decrease in aggregate datasets from Fall and Winter. Information about the temporal and spatial variability of modern foraminiferal distributions was formally incorporated into the Bayesian transfer function through informative foraminifera variability priors and was applied to a Common Era relative sea-level record in New Jersey. The average difference in paleomarsh elevation estimates and uncertainties using an informative vs uninformative prior was minimal

Mass-flowering crops have a greater impact than semi-natural habitat on crop pollinators and pollen deposition

Context: Maximising insect pollination of mass-flowering crops is a widely-discussed approach to sustainable agriculture. Management actions can target landscape-scale semi-natural habitat, cropping patterns or field-scale features, but little is known about their relative effectiveness. Objective: To test how landscape composition (area of mass-flowering crops and semi-natural habitat) and field-scale habitat (margins and hedges) affect pollinator species richness, abundance, and pollen deposition within crop fields. Methods: We surveyed all flower visitors (Diptera, Coleoptera and Hymenoptera) in oilseed rape fields and related them to landscape composition and field features. Flower visitors were classified as bees, non-bee pollinators and brassica specialists. Total pollen deposition by individual taxa was estimated using single visit pollen deposition on stigmas combined with insect abundance. Results: The area of mass-flowering crop had a negative effect on the species richness and abundance of bees in fields, but not other flower visitors. The area of semi-natural habitat in the surrounding landscape had a positive effect on bees, but was not as important as the area of mass-flowering crop. Taxonomic richness and abundance varied significantly between years for non-bee pollinators. Greater cover of mass-flowering crops surrounding fields had a negative effect on pollen deposition, but only when non-bee pollinator numbers were reduced. Conclusions: Management choices that result in landscape homogenisation, such as large areas of mass-flowering crops, may reduce pollination services by reducing the numbers of bees visiting fields. Non-bee insect pollinators may buffer these landscape effects on pollen deposition, and management to support their populations should be considered

Author Correction: Estimating global mean sea-level rise and its uncertainties by 2100 and 2300 from an expert survey

Correction to: NPJ Climate and Atmospheric Science https://doi.org/10.1038/s41612-020-0121-5, published online 08 May 202

EHD-driven mass transport enhancement in surface dielectric barrier discharges

Surface Dielectric Barrier Discharges (S-DBDs) have received renewed attention in recent years for their potential application in emerging biomedical, environmental and agricultural applications. In most of these applications, the plasma is not in direct contact with the substrate being treated and the transport of reactive species from the plasma to the substrate is typically assumed to be controlled by diffusion. Here, we demonstrate that generally this is not the case and that electrohydrodynamic (EHD) forces can produce jets that enhance the delivery of these species, thereby influencing the efficacy of the S-DBD device. In particular, we have studied the degradation of potassium indigotrisulfonate solutions exposed to S-DBDs generated in devices with annular electrodes of diameters varying between 10mm and 50mm. All the devices were driven at constant linear power density (Watts per cm of plasma length) and although local plasma properties remained the same in all the devices, a three-fold efficacy enhancement was observed for devices of diameter ~30mm due to EHD effects

Estimating global mean sea-level rise and its uncertainties by 2100 and 2300 from an expert survey

Sea-level rise projections and knowledge of their uncertainties are vital to make informed mitigation and adaptation decisions. To elicit projections from members of the scientific community regarding future global mean sea-level (GMSL) rise, we repeated a survey originally conducted five years ago. Under Representative Concentration Pathway (RCP) 2.6, 106 experts projected a likely (central 66% probability) GMSL rise of 0.30–0.65 m by 2100, and 0.54–2.15 m by 2300, relative to 1986–2005. Under RCP 8.5, the same experts projected a likely GMSL rise of 0.63–1.32 m by 2100, and 1.67–5.61 m by 2300. Expert projections for 2100 are similar to those from the original survey, although the projection for 2300 has extended tails and is higher than the original survey. Experts give a likelihood of 42% (original survey) and 45% (current survey) that under the high-emissions scenario GMSL rise will exceed the upper bound (0.98 m) of the likely range estimated by the Fifth Assessment Report of the Intergovernmental Panel on Climate Change, which is considered to have an exceedance likelihood of 17%. Responses to open-ended questions suggest that the increases in upper-end estimates and uncertainties arose from recent influential studies about the impact of marine ice cliff instability on the meltwater contribution to GMSL rise from the Antarctic Ice Sheet. © 2020, The Author(s)