THE ROLE OF THE INTERCELLULAR DIFFUSION CHANNELS IN THE REGULATION OF THE DIFFERENTIATED CELLS PHENOTYPE

The comparative investigation of the intercellular contacts in the normal liver, the hepatomas of the different origin and in the liver tissue in the cource of cancerogenesis, has been performed. For the first time it has been shown, that the transformed fibroblasts acquire the sensitivity to the cancerogene at its local interaction with the normal fibroblasts. In the culture of hepatocytes of the grown-up mice liver, first the distinct reverse correlation has been detected between the level of the intercellular bond and the number of the cells, synthsizing the alpha-phetoprotein. The different sensitivity of cells HeLa, transficated with connexines Cx32 and Cx26, to the inhibiting action of CO*002, has been detected.Available from VNTIC / VNTIC - Scientific & Technical Information Centre of RussiaSIGLERURussian Federatio

УПРАВЛІННЯ ПРІОРИТЕТАМИ ПРОЕКТУ НА ОСНОВІ НЕЧІТКОЇ ЛОГІКИ

Based on project priorities, a methodology has been developed to determine the economic efficiency of the project. The technique makes it possible to evaluate priority levels using fuzzy logic based on the MATLAB toolkit and allows you to visualize the state of the system.На основі пріоритетів проекту розроблено методику для визначення оцінки економічної ефективності проекту. Методика дає можливість оцінювати рівні пріоритетів з використанням нечіткої логіки на базі інструментарію MATLAB і дозволяє наочно уявити стан системи

Dynamic regulation of connexins in stem cell pluripotency

©AlphaMed Press 2019 Characterization of the pluripotent “ground state” has led to a greater understanding of species-specific stem cell differences and has imparted an appreciation of the pluripotency continuum that exists in stem cells in vitro. Pluripotent stem cells are functionally coupled via connexins that serve in gap junctional intercellular communication (GJIC) and here we report that the level of connexin expression in pluripotent stem cells depends upon the state in which stem cells exist in vitro. Human and mouse pluripotent stem cells stabilized in a developmentally primitive or “naïve” state exhibit significantly less connexin expression compared with stem cells which are “primed” for differentiation. This dynamic connexin expression pattern may be governed, in part, by differential regulation by pluripotency transcription factors expressed in each cell state. Species-specific differences do exist, however, with mouse stem cells expressing several additional connexin transcripts not found in human pluripotent stem cells. Moreover, pharmacological inhibition of GJIC shows limited impact on naïve human stem cell survival, self-renewal, and pluripotency but plays a more significant role in primed human pluripotent stem cells. However, CRISPR-Cas9 gene ablation of Cx43 in human and mouse primed and naïve pluripotent stem cells reveals that Cx43 is dispensable in each of these four pluripotent stem cell types

Connexin43 Mutant Patient-Derived Induced Pluripotent Stem Cells Exhibit Altered Differentiation Potential

© 2017 American Society for Bone and Mineral Research We present for the first time the generation of induced pluripotent stem cells (iPSCs) from a patient with a connexin-linked disease. The importance of gap junctional intercellular communication in bone homeostasis is exemplified by the autosomal dominant developmental disorder oculodentodigital dysplasia (ODDD), which is linked to mutations in the GJA1 (Cx43) gene. ODDD is characterized by craniofacial malformations, ophthalmic deficits, enamel hypoplasia, and syndactyly. In addition to harboring a Cx43 p.V216L mutation, ODDD iPSCs exhibit reduced Cx43 mRNA and protein abundance when compared to control iPSCs and display impaired channel function. Osteogenic differentiation involved an early, and dramatic downregulation of Cx43 followed by a slight upregulation during the final stages of differentiation. Interestingly, osteoblast differentiation was delayed in ODDD iPSCs. Moreover, Cx43 subcellular localization was altered during chondrogenic differentiation of ODDD iPSCs compared to controls and this may have contributed to the more compact cartilage pellet morphology found in differentiated ODDD iPSCs. These studies highlight the importance of Cx43 expression and function during osteoblast and chondrocyte differentiation, and establish a potential mechanism for how ODDD-associated Cx43 mutations may have altered cell lineages involved in bone and cartilage development. © 2017 American Society for Bone and Mineral Research