(λ,μ)-fuzzy Subrings and (λ,μ)-fuzzy Quotient Subrings with Operators

In this paper, we extend the fuzzy subrings with operators to the (λ, μ)-fuzzy subrings with operators. And the concepts of the (λ, μ)-fuzzy subring with operators and (λ, μ)-fuzzy quotient ring with operators are gived, while their elementary properties are discussed

Fuzzy Subalgebras and Fuzzy Ideals of BCI-Algebras with Operators

The aim of this paper is to introduce the concepts of fuzzy subalgebras, fuzzy ideals and fuzzy quotient algebras of BCI-algebras with operators, and to investigate their basic properties

(λ, μ)-Intuitionistic Fuzzy Subgroups of Groups with Operators

The aim of this paper is to introduce the concepts of the (λ, μ)-intuitionistic fuzzy subgroups and (λ, μ)-intuitionistic fuzzy normal subgroups of groups with operators, and to investigate their properties and characterizations based on M-group homomorphism

Intuitionistic Fuzzy Subalgebras (Ideals) with Thresholds (λ,μ) of BCI-algebras

Based on the theory of intuitionistic fuzzy sets, the concepts of intuitionistic fuzzy subalgebras with thresholds (λ,μ) and intuitionistic fuzzy ideals with thresholds (λ,μ) of BCI-algebras are introduced and some properties of them are discussed

Passenger and energy-saving oriented train timetable and stop plan synchronization optimization model

The stability of train timetables plays an important role in ensuring the safety and punctuality of train operations. Meanwhile, a reasonable stop plan has a significant effect on passenger and energy consumption. Existing research has focused exclusively on adjusting timetables after delays occur, or on adjusting them without considering stop plans. By contrast, in this study, we developed a train timetable and stop plan synchronization optimization model that is passenger and energy-saving oriented. The following objectives were considered for minimization: probability of train delays, energy consumption, and travel time of the trains. A parallelism selection genetic algorithm was designed to solve the model. The section of high-speed railway from Nanjing South to Shanghai Hongqiao was utilized as a case study to evaluate the proposed model. The results showed that, while meeting passenger demand, the proposed optimization approach increased the punctuality rate by 12.55% and decreased the energy consumption by 8.16%

Parental genetic material and oxygen concentration affect hatch dynamics of mouse embryo in vitro

Abstract Background Hatching is crucial for mammalian embryo implantation, since difficulties during this process can lead to implantation failure, ectopic pregnancy and consequent infertility. Despite years of intensive researches, how internal and external factors affecting embryo hatch are still largely unclear. Methods The effects of parental genetic material and oxygen concentration on hatch process were examined. Fertilized and parthenogenetic mouse preimplantation embryos were cultured in vitro under 5 and 20% oxygen for 120 h. Zona pellucida drilling by Peizo micromanipulation were performed to resemble the breach by sperm penetration. Results Firstly, parthenogenetic embryos had similarly high blastocyst developmental efficiency as fertilized embryos, but significantly higher hatch ratio than fertilized embryos in both O2 concentrations. 5% O2 reduced the hatch rate of fertilized embryos from 58.2 to 23.8%, but increased that of parthenogenetic embryos from 81.2 to 90.8% significantly. Analogously, 5% O2 decreased the ratio of Oct4-positive cells in fertilized blastocysts, whereas increased that in parthenogenetic blastocysts. Additionally, 5% O2 increased the total embryonic cell number in both fertilized and parthegenetic embryos, when compared to 20% O2, and the total cell number of fertilized embryos was also higher than that of parthegenetic embryos, despite O2 concentration. Real-time PCR revealed that the expression of key genes involving in MAPK pathway and superoxide dismutase family might contribute to preimplantation development and consequent blastocyst hatch in vitro. Finally, we showed that fertilized and parthenogenetic embryos have diverse hatch dynamics in vitro, although the zona pellucida integrity is not the main reason for their mechanistic differences. Conclusion Both parental genetic material and O2 concentration, as the representative of intrinsic and extrinsic factors respectively, have significant impacts on mouse preimplantation development and subsequent hatch dynamics, probably by regulating the gene expression involving in MAPK pathway and superoxide dismutase family to control embryonic cell proliferation and allocation of ICM cells

Nanofibrous polypeptide hydrogels with collagen-like structure as biomimetic extracellular matrix

Abstract Supramolecular peptides exhibit obvious similarities with collagen fibers in terms of self-assembly characteristics, nanofibrous structure, and responsiveness to external stimuli. Here, a series of supramolecular peptides were developed by altering the amino acid sequence, enabling the self-assembly of three types of 4-biphenylacetic acid (BPAA)-tripeptides into fibrous hydrogel through hydrogen bonding and π–π stacking under the influence of ion induction. Transmission electron and scanning electron microscopies revealed that the diameter of the fiber within nanofibrous hydrogels was ~ 10 and ~ 40 nm, respectively, which was similar with the self-assembled collagen fibers. For this reason, these hydrogels could be considered as a biomimetic extracellular substitute. Meanwhile, the gelation concentration induced by ions was even lower than 0.66 wt%, with an elastic modulus of ~ 0.27 kPa, corresponding to a water content of 99.34 wt%. In addition, the three supramolecular hydrogels were found to be good substrates for L929 cell adhesion and MC-3T3 cell proliferation. The overall results implied that BPAA-based hydrogels have a lucrative application potential as cell carriers. Graphical Abstrac

Molecular co-assembled strategy tuning protein conformation for cartilage regeneration

Abstract The assembly of oligopeptide and polypeptide molecules can reconstruct various ordered advanced structures through intermolecular interactions to achieve protein-like biofunction. Here, we develop a “molecular velcro”-inspired peptide and gelatin co-assembly strategy, in which amphiphilic supramolecular tripeptides are attached to the molecular chain of gelatin methacryloyl via intra-/intermolecular interactions. We perform molecular docking and dynamics simulations to demonstrate the feasibility of this strategy and reveal the advanced structural transition of the co-assembled hydrogel, which brings more ordered β-sheet content and 10-fold or more compressive strength improvement. We conduct transcriptome analysis to reveal the role of co-assembled hydrogel in promoting cell proliferation and chondrogenic differentiation. Subcutaneous implantation evaluation confirms considerably reduced inflammatory responses and immunogenicity in comparison with type I collagen. We demonstrate that bone mesenchymal stem cells-laden co-assembled hydrogel can be stably fixed in rabbit knee joint defects by photocuring, which significantly facilitates hyaline cartilage regeneration after three months. This co-assembly strategy provides an approach for developing cartilage regenerative biomaterials

Additional file 1: of Parental genetic material and oxygen concentration affect hatch dynamics of mouse embryo in vitro

Real time PCR primers for genes expression analysis of 96 h blastocysts. (DOC 41 kb