A Positioning Theory Analysis of Interaction Surrounding Design Failures in an Elementary Engineering Club

This qualitative study applies Positioning Theory to identify positions that mediate the experiences of design failure within the context of an afterschool engineering club (EC) with elementary students diverse in language, race, ethnicity, gender, and academic abilities. We ask: (1) What kinds of structural design failure and failure responses did participants in EC experience? and (2) What are students’ and teachers’ positions in relation to responses to design failure? Types of positions (e.g., builder, tinkerer, idea-elicitor, director, observer) were identified in relation to children’s and teachers’ actions and speech in response to structural design failure during EC. Participants included 12 third-grade students and four teachers involved in EC for eight weeks. Data sources include audio transcripts, video, and field notes. Twenty-four design failure episodes were identified and transcribed multimodally from video, followed by coding of episodes using a multimodal Positioning Theory analytical framework. Findings discuss the kinds of engineering design actions and associated positionings unfolding in response to failure as well as the positions mediating teacher and student responses to design. We highlight the importance of student and teacher mediation as well as how Positioning Theory can be used to expand our understanding of (re)positionings that can occur within responses to design failure. Specifically, elementary engineering curricular materials must create the context to support the range of positions taken up in response to design failure. This includes explicit modeling of discursive actions surrounding design failure, multiple opportunities for students to experience and respond to design failure with time to improve beyond the design–build–test model, and support for teachers to address the range of students’ responses to design failures knowledgeably and flexibly

Performance: An Approach to Strengthening Interdisciplinarity in Women\u27s Studies and Gender Studies

Issues of transition are always difficult and transforming founding assumptions to suit changing conditions can be a challenge for Women’s Studies and Gender Studies programs in the corporate university environment. This essay explains how performance of Fefu and Her Friends by Marie Irene Fornes in a mansion on campus led faculty participants in History, Classics, Modern Languages, Theatre, Communication, Social Work, and Nursing to re-vision their professional lives and the institutional status of the Women’s Studies and Gender Studies program through a new appreciation for interdisciplinarity, derived from using performance as research. Each of eight faculty reflect on their experience of performance as an embodied pedagogy and method of research. The importance of emotion, trust, and responsibility in team work and collaboration, consideration of bodies in spaces and communities, and linkages between theory to practice are all considered in reflections. The individual re-visioning led to changes and renewed energy in the WSGS program and faculty collaboration in research and teaching

Independent and Interactive Contributions of Parenting Behaviors and Beliefs in the Prediction of Early Childhood Behavior Problems

This study examined interactions between parenting beliefs and parenting behaviors in the prediction of early childhood externalizing and internalizing symptom

The altered gut microbiota in adults with cystic fibrosis

peer-reviewedBackground Cystic Fibrosis (CF) is an autosomal recessive disease that affects the function of a number of organs, principally the lungs, but also the gastrointestinal tract. The manifestations of cystic fibrosis transmembrane conductance regulator (CFTR) dysfunction in the gastrointestinal tract, as well as frequent antibiotic exposure, undoubtedly disrupts the gut microbiota. To analyse the effects of CF and its management on the microbiome, we compared the gut microbiota of 43 individuals with CF during a period of stability, to that of 69 non-CF controls using 454-pyrosequencing of the 16S rRNA gene. The impact of clinical parameters, including antibiotic therapy, on the results was also assessed. Results The CF-associated microbiome had reduced microbial diversity, an increase in Firmicutes and a reduction in Bacteroidetes compared to the non-CF controls. While the greatest number of differences in taxonomic abundances of the intestinal microbiota was observed between individuals with CF and the healthy controls, gut microbiota differences were also reported between people with CF when grouped by clinical parameters including % predicted FEV1 (measure of lung dysfunction) and the number of intravenous (IV) antibiotic courses in the previous 12 months. Notably, CF individuals presenting with severe lung dysfunction (% predicted FEV1 ≤ 40%) had significantly (p < 0.05) reduced gut microbiota diversity relative to those presenting with mild or moderate dysfunction. A significant negative correlation (−0.383, Simpson’s Diversity Index) was also observed between the number of IV antibiotic courses and gut microbiota diversity. Conclusions This is one of the largest single-centre studies on gut microbiota in stable adults with CF and demonstrates the significantly altered gut microbiota, including reduced microbial diversity seen in CF patients compared to healthy controls. The data show the impact that CF and it's management have on gut microbiota, presenting the opportunity to develop CF specific probiotics to minimise microbiota alterations.The authors and their work were supported by the Science Foundation of Ireland and funded by the Centre for Science, Engineering and Technology (SFI-CSET) grant 02/CE/B124 and by FP7 funded CFMATTERS (Cystic Fibrosis Microbiome-determined Antibiotic Therapy Trial in Exacerbations: Results Stratified, Grant Agreement no. 603038)

Carbohydrate Syntrophy enhances the establishment of Bifidobacterium breve UCC2003 in the neonatal gut

The non-digestible oligosaccharide fraction of maternal milk represents an important of carbohydrate and energy source for saccharolytic bifidobacteria in the gastrointestinal tract during early life. However, not all neonatal bifidobacteria isolates can directly metabolise the complex sialylated, fucosylated, sulphated and/or N-acetylglucosamine-containing oligosaccharide structures present in mothers milk. For some bifidobacterial strains, efficient carbohydrate syntrophy or crossfeeding is key to their establishment in the gut. In this study, we have adopted advanced functional genomic approaches to create single and double in-frame deletions of the N-acetyl glucosamine 6-phosphate deacetylase encoding genes, nagA1 and nagA2, of B. breve UCC2003. In vitro phenotypic analysis followed by in vivo studies on co-colonisation, mother to infant transmission, and evaluation of the relative co-establishment of B. bifidum and B. breve UCC2003 or UCC2003 Delta nagA1 Delta nagA2 in dam-reared neonatal mice demonstrates the importance of crossfeeding on sialic acid, fucose and N-acetylglucosamine-containing oligosaccharides for the establishment of B. breve UCC2003 in the neonatal gut. Furthermore, transcriptomic analysis of in vivo gene expression shows upregulation of genes associated with the utilisation of lactose, sialic acid, GlcNAc-6-S and fucose in B. breve UCC2003, while for UCC2003 Delta nagA1 Delta nagA2 only genes for lactose metabolism were upregulated

Disciplinary Literacies Across Content Areas: Supporting Secondary Reading Through Functional Language Analysis

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/88105/1/JAAL.53.7.6.pd

A pilot study demonstrating the altered gut microbiota functionality in stable adults with Cystic Fibrosis

peer-reviewedCystic Fibrosis (CF) and its treatment result in an altered gut microbiota composition compared to non-CF controls. However, the impact of this on gut microbiota functionality has not been extensively characterised. Our aim was to conduct a proof-of-principle study to investigate if measurable changes in gut microbiota functionality occur in adult CF patients compared to controls. Metagenomic DNA was extracted from faecal samples from six CF patients and six non-CF controls and shotgun metagenomic sequencing was performed on the MiSeq platform. Metabolomic analysis using gas chromatography-mass spectrometry was conducted on faecal water. The gut microbiota of the CF group was significantly different compared to the non-CF controls, with significantly increased Firmicutes and decreased Bacteroidetes. Functionality was altered, with higher pathway abundances and gene families involved in lipid (e.g. PWY 6284 unsaturated fatty acid biosynthesis (p = 0.016)) and xenobiotic metabolism (e.g. PWY-5430 meta-cleavage pathway of aromatic compounds (p = 0.004)) in CF patients compared to the controls. Significant differences in metabolites occurred between the two groups. This proof-of-principle study demonstrates that measurable changes in gut microbiota functionality occur in CF patients compared to controls. Larger studies are thus needed to interrogate this further

The evolution of health policy guidelines for assisted reproduction in the Republic of Ireland, 2004-2009

This analysis reports on Irish regulatory policies for in vitro fertilisation (IVF) from 2004-2009, in the context of membership changes within the Medical Council of Ireland. To achieve this, the current (2009) edition of the Guide to Professional Conduct & Ethics was compared with the immediately preceding version (2004). The statutory composition of the Medical Council from 2004-2009 was also studied. Content analysis of the two editions identified the following differences: 1) The 2004 guide states that IVF "should only be used after thorough investigation has failed to reveal a treatable cause of the infertility", while the 2009 guide indicates IVF "should only be used after thorough investigation has shown that no other treatment is likely to be effective"; 2) The 2004 stipulation stating that fertilized ovum (embryo) "must be used for normal implantation and must not be deliberately destroyed" is absent from the 2009 guidelines; 3) The option to donate "unused fertilised ova" (embryos) is omitted from the 2009 guidelines; 4) The 2009 guidelines state that ART should be offered only by "suitably qualified professionals, in appropriate facilities, and according to the international best practice"; 5) The 2009 guidelines introduce criteria that donations as part of a donor programme should be "altruistic and non-commercial". These last two points represent original regulatory efforts not appearing in the 2004 edition. The Medical Practitioners Act 2007 reduced the number of physicians on the Medical Council to 6 (of 25) members. The ethical guidelines from 2004 preceded this change, while the reconstituted Medical Council published the 2009 version. Between 2004 and 2009, substantial modifications in reproductive health policy were incorporated into the Medical Council's ethical guidelines. The absence of controlling Irish legislation means that patients and IVF providers in Ireland must rely upon these guidelines by default. Our critique traces the evolution of public policy on IVF during a time when the membership of the Medical Council changed radically; reduced physician contribution to decision-making was associated with diminished protection for IVF-derived embryos in Ireland. Considerable uncertainty on IVF practice in Ireland remains