Investigating the evolution of apoptosis in malaria parasites: the importance of ecology

Apoptosis is a precisely regulated process of cell death which occurs widely in multicellular organisms and is essential for normal development and immune defences. In recent years, interest has grown in the occurrence of apoptosis in unicellular organisms. In particular, as apoptosis has been reported in a wide range of species, including protozoan malaria parasites and trypanosomes, it may provide a novel target for intervention. However, it is important to understand when and why parasites employ an apoptosis strategy before the likely long-and short-term success of such an intervention can be evaluated. The occurrence of apoptosis in unicellular parasites provides a challenge for evolutionary theory to explain as organisms are expected to have evolved to maximise their own proliferation, not death. One possible explanation is that protozoan parasites undergo apoptosis in order to gain a group benefit from controlling their density as this prevents premature vector mortality. However, experimental manipulations to examine the ultimate causes behind apoptosis in parasites are lacking. In this review, we focus on malaria parasites to outline how an evolutionary framework can help make predictions about the ecological circumstances under which apoptosis could evolve. We then highlight the ecological considerations that should be taken into account when designing evolutionary experiments involving markers of cell death, and we call for collaboration between researchers in different fields to identify and develop appropriate markers in reference to parasite ecology and to resolve debates on terminology.Host-parasite interactio

The Serological Findings of Parvo virus B19 and Neopterin Detection among Sickle cell Disease Patients and Blood Doners in the Kingdom of Bahrain

Introduction. Parvovirus B19 (PV B19) is a small, non-enveloped, ss DNA virus with an icosahedral capsid having a size of 18–26 nm. PV B19 transmits through respiratory droplets, blood transfusion and nosocomial infections that have also been documented recently. The virus targets the actively dividing Erythroid Progenitor Cells (EPCs) that are found in the human bone marrow, fetal liver and human umbilical cord. Methods. The study was particularly conducted on Sickle Cell Disease (SCD) patients and focuses on the determination of parvovirus B19 among Bahraini population by relying on their clinical status. The serological study of PV B19 was performed using Enzyme Linked Immunosorbent Assay (ELISA) technique and includes 150 SCD patientsand 100 healthy blood donors in which both males and females were employed. The samples were taken from the emergency unit of Salmania Medical Complex (SMC) and Ibrahim Khalil Kano Center (IKKC). Results. Of the 150 SCD patients, 100 were with vaso-occlusive crisis(VOC) and 50 non-vaso-occlusive crisis (NVOC). The three groups showed significantly higher percentages of PV B19 IgG but the percentage in SCD was relatively high compared to the control group of age-matched healthy donors – 70% of the VOC patients, 76% of the NVOC cases and 57% of blood donors were found to be IgG sero-positive. Discussion and Conclusion. PV B19 is a pathogenic virus and sometimes considered as life-threatening specifically for those individuals who have SCD due to which a risk of transient aplastic crisis increases. This virus is only associated with those patients whohave some hematological disorders such as hemolytic anemia and erythro-cytopenia. An effective screening test must be performed in the future to reduce the risk of PV B19 infection. Keywords: Aplastic Crisis, Seroprevalence, Bahraini Population, Genotypes, Vasoocclusive crisis, Neopteri

Parallel H.264/AVC Fast Rate-Distortion Optimized Motion Estimation using Graphics Processing Unit and Dedicated Hardware

Heterogeneous systems on a single chip composed of CPU, Graphical Processing Unit (GPU), and Field Programmable Gate Array (FPGA) are expected to emerge in near future. In this context, the System on Chip (SoC) can be dynamically adapted to employ different architectures for execution of data-intensive applications. Motion estimation is one such task that can be accelerated using FPGA and GPU for high performance H.264/AVC encoder implementation. In most of works on parallel implementation of motion estimation, the bit rate cost of motion vectors is generally ignored. On the contrary, this paper presents a fast rate-distortion optimized parallel motion estimation algorithm implemented on GPU using OpenCL and FPGA/ASIC using VHDL. The predicted motion vectors are estimated from temporally preceding motion vectors and used for evaluating the bit rate cost of the motion vectors simultaneously. The experimental results show that the proposed scheme achieves significant speedup on GPU and FPGA, and has comparable ratedistortion performance with respect to sequential fast motion estimation algorithm

Effect of fulvic acids on lead-induced oxidative stress to metal sensitive Vicia faba L. plant

Lead (Pb) is a ubiquitous environmental pollutant capable to induce various morphological, physiological, and biochemical functions in plants. Only few publications focus on the influence of Pb speciation both on its phytoavailability and phytotoxicity. Therefore, Pb toxicity (in terms of lipid peroxidation, hydrogen peroxide induction, and photosynthetic pigments contents) was studied in Vicia faba plants in relation with Pb uptake and speciation. V. faba seedlings were exposed to Pb supplied as Pb(NO3)2 or complexed by two fulvic acids (FAs), i.e. Suwannee River fulvic acid (SRFA) and Elliott Soil fulvic acid (ESFA), for 1, 12, and 24 h under controlled hydroponic conditions. For both FAs, Pb uptake and translocation by Vicia faba increased at low level (5 mg l−1), whereas decreased at high level of application (25 mg l−1). Despite the increased Pb uptake with FAs at low concentrations, there was no influence on the Pb toxicity to the plants. However, at high concentrations, FAs reduced Pb toxicity by reducing its uptake. These results highlighted the role of the dilution factor for FAs reactivity in relation with structure; SRFA was more effective than ESFA in reducing Pb uptake and alleviating Pb toxicity to V. faba due to comparatively strong binding affinity for the heavy metal

Long-term field metal extraction by pelargonium:phytoextraction efficiency in relation to plant maturity

The long length of periods required for effective soil remediation via phytoextraction constitutes a weak point that reduces its industrial use. However, these calculated periods are mainly based on short-term and/or hydroponic controlled experiments. Moreover, only a few studies concern more than one metal, although soils are scarcely polluted by only one element.In this scientific context, the phytoextraction of metals and metalloids (Pb, Cd, Zn, Cu,and As) by Pelargonium was measured after a long-term field experiment. Both bulk and rhizosphere soils were analyzed in order to determine the mechanisms involved in soil-root transfer. First, a strong increase in lead phytoextraction was observed with plant maturity, significantly reducing the length of the period required for remediation. Rhizosphere Pb, Zn, Cu, Cd, and As accumulation was observed (compared to bulk soil), indicating metal mobilization by the plant, perhaps in relation to root activity. Moreover, metal phytoextraction and translocation were found to be a function of the metals’ nature. These results, taken altogether, suggest that Pelargonium could be used as a multi-metal hyperaccumulator under multi-metal soil contamination conditions, and they also provide an interesting insight for improving field phytoextraction remediation in terms of the length of time required, promoting this biological technique

Gauged linear sigma model and pion-pion scattering

A simple gauged linear sigma model with several parameters to take the symmetry breaking and the mass differences between the vector meson and the axial vector meson into account is considered here as a possibly useful template for the role of a light scalar in QCD as well as for (at a different scale) an effective Higgs sector for some recently proposed walking technicolor models. An analytic procedure is first developed for relating the Lagrangian parameters to four well established (in the QCD application) experimental inputs. One simple equation distinguishes three different cases:1. QCD with axial vector particle heavier than vector particle, 2. possible technicolor model with vector particle heavier than the axial vector one, 3. the unphysical QCD case where both the KSRF and Weinberg relations hold. The model is applied to the s-wave pion-pion scattering in QCD. Both the near threshold region and (with an assumed unitarization) theglobal region up to about 800 MeV are considered. It is noted that there is a little tension between the choice of bare sigma mass parameter for describing these two regions. If a reasonable globa fit is made, there is some loss of precision in the near threshold region.Comment: 19 pages, 9 figure

Machine learning and multi-dimension features based adaptive intrusion detection in ICN

As a new network architecture, Information-Centric Networks (ICN) has great advantages in content distribution and can better meet our needs. But it faced with many threats unavoidably. There are four types of attack in ICN: naming related attacks, routing related attacks, caching related attacks and miscellaneous attacks. These attacks will undermine the availability of ICN, the confidentiality and privacy of data. In addition, routers store a large amount of content for the users' request, and it is necessary to protect these intermediate nodes. Since the styles of content stored in nodes are not the same, using a unified set of intrusion detection rules simply will cause a large number of false positives and false negatives. Therefore, every node should perform intrusion detection according to its own characteristics. In this paper, we propose an intrusion detection mechanism to alert for abnormal packets. We introduce a extensive solution using machine learning for attacks in ICN. Moreover, the nodes in this scheme can adapt to the external environment and intelligently detect packets. Simulation on the machine learning algorithm involved prove that the algorithm is effective and suitable for network packets

Molecular Characterization and Functional Significance of the Vti Family of SNARE Proteins in Tick Salivary Glands

Exocytosis involves membrane fusion between secretory vesicles and the plasma membrane. The Soluble N-ethylmaleimide-sensitive factor attachment proteins (SNAPs) and their receptor proteins (SNAREs) interact to fuse vesicles with the membrane and trigger the release of their sialosecretome out of the tick salivary gland cells. In this study, we examined the functional significance of the Vti family of SNARE proteins of blood-feeding Amblyomma maculatum and Amblyomma americanum. Vti1A and Vti1B have been implicated in multiple functional roles in vesicle transport. QRT-PCR studies demonstrated that the highest transcriptional expression of vti1a and vti1b genes occurs in unfed salivary glands, suggesting that elevated secretory vesicle formation occurs prior to feeding but continues at low rates after blood feeding commences. Vti1A and Vti1B localize to the secretory vesicles in unfed tick salivary glands in immunofluorescence microscopy studies. Knockdown of vti1a and vti1b by RNA interference resulted in a significant decrease in the engorged tick weight compared to the control during prolonged blood-feeding on the host. RNA interference of vti1a or vti1b impaired oviposition and none of the ticks produced eggs masses. Surprisingly, the double knockdown did not produce a strong phenotype and ticks fed normally on the host and produced egg masses, suggesting a compensatory mechanism exists within the secretory system which may have been activated in the double knockdown. These results suggest an important functional role of the Vti family of SNARE proteins in tick blood feeding and ultimately oviposition. Understanding the basic functions of the Vti family of SNARE proteins in salivary glands may lead to better ways to prevent tick attachment and transmission of tick-borne diseases

Key features of palliative care service delivery to Indigenous peoples in Australia, New Zealand, Canada and the United States: A comprehensive review

Background: Indigenous peoples in developed countries have reduced life expectancies, particularly from chronic diseases. The lack of access to and take up of palliative care services of Indigenous peoples is an ongoing concern. Objectives: To examine and learn from published studies on provision of culturally safe palliative care service delivery to Indigenous people in Australia, New Zealand (NZ), Canada and the United States of America (USA); and to compare Indigenous peoples’ preferences, needs, opportunities and barriers to palliative care. Methods: A comprehensive search of multiple databases was undertaken. Articles were included if they were published in English from 2000 onwards and related to palliative care service delivery for Indigenous populations; papers could use quantitative or qualitative approaches. Common themes were identified using thematic synthesis. Studies were evaluated using Daly’s hierarchy of evidence-for-practice in qualitative research. Results: Of 522 articles screened, 39 were eligible for inclusion. Despite diversity in Indigenous peoples’ experiences across countries, some commonalities were noted in the preferences for palliative care of Indigenous people: to die close to or at home; involvement of family; and the integration of cultural practices. Barriers identified included inaccessibility, affordability, lack of awareness of services, perceptions of palliative care, and inappropriate services. Identified models attempted to address these gaps by adopting the following strategies: community engagement and ownership; flexibility in approach; continuing education and training; a whole-of-service approach; and local partnerships among multiple agencies. Better engagement with Indigenous clients, an increase in number of palliative care patients, improved outcomes, and understanding about palliative care by patients and their families were identified as positive achievements. Conclusions: The results provide a comprehensive overview of identified effective practices with regards to palliative care delivered to Indigenous populations to guide future program developments in this field. Further research is required to explore the palliative care needs and experiences of Indigenous people living in urban areas

Inflation and Transition to a Slowly Accelerating Phase from S.S.B. of Scale Invariance

We consider the effects of adding a scale invariant $R^{2}$ term to the action of the scale invariant model (SIM) studied previously by one of us (E.I.G., Mod. Phys. Lett. A14, 1043 (1999)). The SIM belongs to the general class of theories, where an integration measure independent of the metric is introduced. To implement scale invariance (S.I.), a dilaton field is introduced. The integration of the equations of motion associated with the new measure gives rise to the spontaneous symmetry breaking (S.S.B) of S.I.. After S.S.B. of S.I. in the model with the $R^{2}$ term, it is found that a non trivial potential for the dilaton is generated. This potential contains two flat regions: one associated with the Planck scale and with an inflationary phase, while the other flat region is associated to a very small vacuum energy (V.E.) and is associated to the present slowly accelerated phase of the universe (S.A.PH). The smallness of the V.E. in the S.A.PH. is understood through the see saw mechanism introduced in S.I.M.Comment: 22 pages, latex, three figures now in separate file