Radiation damage in silicon first semiannual report, oct. 15, 1963 - apr. 15, 1964

Observed paramagnetic center, effects of impurities on radiation damage of silicon, and low energy proton bombardment of silicon and gallium arsenide solar cell

Distinct fos-expressing neuronal ensembles in the ventromedial prefrontal cortex mediate food reward and extinction memories

In operant learning, initial reward-associated memories are thought to be distinct from subsequent extinction-associated memories. Memories formed during operant learning are thought to be stored in “neuronal ensembles.” Thus, we hypothesize that different neuronal ensembles encode reward- and extinction-associated memories. Here, we examined prefrontal cortex neuronal ensembles involved in the recall of reward and extinction memories of food self-administration.Wefirst trained rats to lever press for palatable food pellets for 7 d (1 h/d) and then exposed them to 0, 2, or 7 daily extinction sessions in which lever presses were not reinforced. Twenty-four hours after the last training or extinction session, we exposed the rats to either a short 15 min extinction test session or left them in their homecage (a control condition). We found maximal Fos (a neuronal activity marker) immunoreactivity in the ventral medial prefrontal cortex of rats that previously received 2 extinction sessions, suggesting that neuronal ensembles in this area encode extinction memories. We then used the Daun02 inactivation procedure to selectively disrupt ventral medial prefrontal cortex neuronal ensembles that were activated during the 15 min extinction session following 0 (no extinction) or 2 prior extinction sessions to determine the effects of inactivating the putative food reward and extinction ensembles, respectively, on subsequent nonreinforced food seeking 2 d later. Inactivation of the food reward ensembles decreased food seeking, whereas inactivation of the extinction ensembles increased food seeking. Our results indicate that distinct neuronal ensembles encoding operant reward and extinction memories intermingle within the same cortical area

galign: A Tool for Rapid Genome Polymorphism Discovery

BACKGROUND: Highly parallel sequencing technologies have become important tools in the analysis of sequence polymorphisms on a genomic scale. However, the development of customized software to analyze data produced by these methods has lagged behind. METHODS/PRINCIPAL FINDINGS: Here I describe a tool, 'galign', designed to identify polymorphisms between sequence reads obtained using Illumina/Solexa technology and a reference genome. The 'galign' alignment tool does not use Smith-Waterman matrices for sequence comparisons. Instead, a simple algorithm comparing parsed sequence reads to parsed reference genome sequences is used. 'galign' output is geared towards immediate user application, displaying polymorphism locations, nucleotide changes, and relevant predicted amino-acid changes for ease of information processing. To do so, 'galign' requires several accessory files easily derived from an annotated reference genome. Direct sequencing as well as in silico studies demonstrate that 'galign' provides lesion predictions comparable in accuracy to available prediction programs, accompanied by greater processing speed and more user-friendly output. We demonstrate the use of 'galign' to identify mutations leading to phenotypic consequences in C. elegans. CONCLUSION/SIGNIFICANCE: Our studies suggest that 'galign' is a useful tool for polymorphism discovery, and is of immediate utility for sequence mining in C. elegans

Incompatibility of long-period neutron star precession with creeping neutron vortices

Aims: To determine whether ``vortex creep'' in neutron stars, the slow motion of neutron vortices with respect to pinning sites in the core or inner crust, is consistent with observations of long-period precession. Methods: Using the concept of vortex drag, I discuss the precession dynamics of a star with imperfectly-pinned (i.e., "creeping'') vortices. Results: The precession frequency is far too high to be consistent with observations, indicating that the standard picture of the outer core (superfluid neutrons in co-existence with type II, superconducting protons) should be reconsidered. There is a slow precession mode, but it is highly over-damped and cannot complete even a single cycle. Moreover, the vortices of the inner crust must be able to move with little dissipation with respect to the solid.Comment: 4 pages, v3. Missing reference adde

Tkachenko waves, glitches and precession in neutron star

Here I discuss possible relations between free precession of neutron stars, Tkachenko waves inside them and glitches. I note that the proposed precession period of the isolated neutron star RX J0720.4-3125 (Haberl et al. 2006) is consistent with the period of Tkachenko waves for the spin period 8.4s. Based on a possible observation of a glitch in RX J0720.4-3125 (van Kerkwijk et al. 2007), I propose a simple model, in which long period precession is powered by Tkachenko waves generated by a glitch. The period of free precession, determined by a NS oblateness, should be equal to the standing Tkachenko wave period for effective energy transfer from the standing wave to the precession motion. A similar scenario can be applicable also in the case of the PSR B1828-11.Comment: 6 pages, no figures, accepted to Ap&S

An accretion disk model for periodic timing variations of pulsars

The long-term, highly periodic and correlated variations in both the pulse shape and the rate of slow-down of two isolated pulsars (PSRs) PSR B1828-11 and PSR B1642-03 were discovered recently. This phenomenon may provide evidence for "free precession" as suggested in the literature. Some authors presented various kinds of models to explain this phenomenon within the framework of free precession. Here we present an accretion disk model for this precession phenomenon instead. Under reasonable parameters, the observed phenomenon can be explained by an isolated pulsar with a fossil disk. This may link radio pulsars and anomalous X-ray pulsars (AXPs) and present an indirect evidence for the existence of the fossil disk in nature.Comment: 4 pages, 2 figures, to appear in A&A Lette

Involvement of Noradrenergic Neurotransmission in the Stress- but not Cocaine-Induced Reinstatement of Extinguished Cocaine-Induced Conditioned Place Preference in Mice: Role for β-2 Adrenergic Receptors

The responsiveness of central noradrenergic systems to stressors and cocaine poses norepinephrine as a potential common mechanism through which drug re-exposure and stressful stimuli promote relapse. This study investigated the role of noradrenergic systems in the reinstatement of extinguished cocaine-induced conditioned place preference by cocaine and stress in male C57BL/6 mice. Cocaine- (15 mg/kg, i.p.) induced conditioned place preference was extinguished by repeated exposure to the apparatus in the absence of drug and reestablished by a cocaine challenge (15 mg/kg), exposure to a stressor (6-min forced swim (FS); 20–25°C water), or administration of the α-2 adrenergic receptor (AR) antagonists yohimbine (2 mg/kg, i.p.) or BRL44408 (5, 10 mg/kg, i.p.). To investigate the role of ARs, mice were administered the nonselective β-AR antagonist, propranolol (5, 10 mg/kg, i.p.), the α-1 AR antagonist, prazosin (1, 2 mg/kg, i.p.), or the α-2 AR agonist, clonidine (0.03, 0.3 mg/kg, i.p.) before reinstatement testing. Clonidine, prazosin, and propranolol failed to block cocaine-induced reinstatement. The low (0.03 mg/kg) but not high (0.3 mg/kg) clonidine dose fully blocked FS-induced reinstatement but not reinstatement by yohimbine. Propranolol, but not prazosin, blocked reinstatement by both yohimbine and FS, suggesting the involvement of β-ARs. The β-2 AR antagonist ICI-118551 (1 mg/kg, i.p.), but not the β-1 AR antagonist betaxolol (10 mg/kg, i.p.), also blocked FS-induced reinstatement. These findings suggest that stress-induced reinstatement requires noradrenergic signaling through β-2 ARs and that cocaine-induced reinstatement does not require AR activation, even though stimulation of central noradrenergic neurotransmission is sufficient to reinstate

Fos-expressing neuronal ensemble in rat ventromedial prefrontal cortex encodes cocaine seeking but not food seeking in rats

Neuronal ensembles in ventromedial prefrontal cortex (vmPFC) play a role in both cocaine and palatable food seeking. However, it is unknown whether similar or different vmPFC neuronal ensembles mediate food and cocaine seeking. Here, we used the Daun02 inactivation procedure to assess whether the neuronal ensembles mediating food and cocaine seeking can be functionally distinguished. We trained male and female Fos-LacZ rats to self-administer palatable food pellets and cocaine on alternating days for 18 days. We then exposed the rats to a brief nonreinforced food- or cocaine-seeking test to induce Fos and β-gal in neuronal ensembles associated with food or cocaine seeking, respectively and infused Daun02 into vmPFC to ablate the β-gal-expressing ensembles. Two days later, we tested the rats for food or cocaine seeking under extinction conditions. Although inactivation of the food-seeking ensemble did not influence food or cocaine seeking, inactivation of the cocaine-seeking ensemble reduced cocaine seeking but not food seeking. Results indicate that the neuronal ensemble activated by cocaine seeking in vmPFC is functionally separate from the ensemble activated by food seeking

Vortex Unpinning in Precessing Neutron Stars

The neutron vortices thought to exist in the inner crust of a neutron star interact with nuclei and are expected to pin to the nuclear lattice. Evidence for long-period precession in pulsars, however, requires that pinning be negligible. We estimate the strength of vortex pinning and show that hydrodynamic forces present in a precessing star are likely sufficient to unpin all of the vortices of the inner crust. In the absence of precession, however, vortices could pin to the lattice with sufficient strength to explain the giant glitches observed in many radio pulsars.Comment: 5 pages, 2 figure

Kinetics of electron-positron pair plasmas using an adaptive Monte Carlo method

A new algorithm for implementing the adaptive Monte Carlo method is given. It is used to solve the relativistic Boltzmann equations that describe the time evolution of a nonequilibrium electron-positron pair plasma containing high-energy photons and pairs. The collision kernels for the photons as well as pairs are constructed for Compton scattering, pair annihilation and creation, bremsstrahlung, and Bhabha & Moller scattering. For a homogeneous and isotropic plasma, analytical equilibrium solutions are obtained in terms of the initial conditions. For two non-equilibrium models, the time evolution of the photon and pair spectra is determined using the new method. The asymptotic numerical solutions are found to be in a good agreement with the analytical equilibrium states. Astrophysical applications of this scheme are discussed.Comment: 43 pages, 7 postscript figures, to appear in the Astrophysical Journa