169 research outputs found
Assessing of growth, antioxidant enzymes, and phytohormone regulation in Cucurbita pepo under cadmium stress
One of the major problems worldwide is soil pollution by trace metal elements, which limits plant productivity and threatens human health. In this work, we have studied the effect of different concentrations of cadmium on Cucurbita pepo plants, evaluating different physiological and biochemical parameters: hormone signaling, metabolite concentration (malondialdehyde and hydrogen peroxide) and, in addition, the antioxidant enzyme activities of catalase and superoxide dismutase were evaluated. The production of biomass decreased under the Cd‐stress. The results showed that C. pepo accumulates higher amounts of Cd2+ in roots than in shoots and fruits. Cd2+ differently affected the content of endogenous phytohormones. Furthermore, data suggest an essential involvement of roots in the regulation of tolerance to trace elements. As a result, indole acetic acid content increased in roots of treated plants, indicating that this phytohormone can stimulate root promotion and growth under Cd‐stress. Similarly, salicylic acid content in roots and shoots increased in response to Cd2+, as well as abscisic acid levels in roots and fruits. In roots, the rambling accumulation pattern observed for jasmonic acid and salicylic acid suggests the lack of a specific regulation role against trace element toxicity. The activity of catalase and superoxide dismutase decreased, disrupted by the metal stress. However, the proline, malondialdehyde and hydrogen peroxide content significantly increased in Cd2+in all the analyzed tissues of the stressed plants. All these data suggest that C. pepo plants are equipped with an effective antioxidant mechanism against oxidative stress induced by cadmium up to a concentration of 500 μM
Management priorities for marine invasive species
Managing invasive alien species is particularly challenging in the ocean mainly because marine ecosystems are highly connected across broad spatial scales. Eradication of marine invasive species has only been achieved when species were detected early, and management responded rapidly. Generalized approaches, transferable across marine regions, for prioritizing actions to control invasive populations are currently lacking. Here, expert knowledge was elicited to prioritize 11 management actions for controlling 12 model species, distinguished by differences in dispersion capacity, distribution in the area to be managed, and taxonomic identity. Each action was assessed using five criteria (effectiveness, feasibility, acceptability, impacts on native communities, and cost), which were combined in an ‘applicability’ metric. Raising public awareness and encouraging the commercial use of invasive species were highly prioritized, whereas biological control actions were considered the least applicable. Our findings can guide rapid decision-making on prioritizing management options for the control of invasive species especially at early stages of invasion, when reducing managers' response time is critical
Guide ROSELT/OSS pour l'évaluation et le suivi des pratiques d'exploitation des ressources naturelles
Barium effect on germination, plant growth, and antioxidant enzymes in Cucumis sativus L. plants
Barium (Ba) is a nonessential element that can cause several deleterious effects in
most organisms. Elevated Ba concentrations can be toxic for plants and may affect
growth and disturbances in homeostasis. This study aimed to evaluate the Ba stress,
the plant-tolerance limits, and the detoxification strategy adopted by Cucumis sativus
L. The effect of Ba on seed's germination and vegetative development of this species
was evaluated. For germination test, different Ba concentrations were used (0, 200,
500, 1,000, and 2,000 μM). Results showed that germination was stimulated with
500 and 2,000 µM of Ba. The toxicity effect on plant development was studied by
treating the plants with increasing doses of Ba (100, 200, 300, and 500 μM) during
45 days. Shoot and root dry biomass production decreased significantly with elevated
Ba concentrations, although water content enhanced in the roots. The concentration
of Ba, 500 µM, induced high Ba accumulation in shoots and roots (9 times higher
than in the control plants). Moreover, results showed that catalase, guaiacol peroxidase, and ascorbate peroxidase activities were stimulated in the different tissues of
cucumber plants which highlight the occurring of an oxidative damage through Ba
treatments and the involvement of the plant enzymatic antioxidant defense system
A multipurpose experimental facility for advanced X-ray Spectrometry applications
Ponencia presentada en la European Conference on X-Ray Spectrometry (EXRS). 2014Motivation, Ultra High Vacuum Chamber (UHVC) project: To support/enhance the training of scientists/engineers from developing countries in the operation of synchrotron radiation instrumentation; To provide beam time access for R&D projects and hands-on training in SR-XRS based techniques; To promote networking and knowledge sharing; To increase the quality and the competitiveness of the developing countries to apply beam time proposals at SR facilities; To contribute in the further development of XRS techniques in applications with socioeconomicalrelevance (characterization of energy storage/conversion materials, environmental, biological and biomedical applications)Fil: Leani, Juan José. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina.Fil: Leani, Juan José. Nuclear Science and Instrumentation Laboratory, IAEA Laboratories; Austria.Física Atómica, Molecular y Química (física de átomos y moléculas incluyendo colisión, interacción con radiación, resonancia magnética, Moessbauer Efecto.
Performance and bacterial community shifts during phosphogypsum biotransformation
Phosphogypsum (PG) is an industrial waste composed mainly by sulfate, turning it a suitable sulfate source for sulfate-reducing bacteria (SRB). In the present work, the capability of two SRB communities, one enriched from Portuguese PG (culture PG) and the other from sludge from a wastewater treatment plant (culture WWT-1), to use sulfate from PG was compared. In addition, the impact of this sulfate-rich waste in the microbial community was assessed. The highest efficiency in terms of sulfate reduction was observed with culture WWT-1. The bacterial composition of this culture was not significantly affected when sodium sulfate from the nutrient medium was replaced by PG as a sulfate source. Next generation sequencing (NGS) showed that this community was phylogenetically diverse, composed by bacteria affiliated to Clostridium, Arcobacter, and Sulfurospirillum genera and by SRB belonging to Desulfovibrio, Desulfomicrobium, and Desulfobulbus genera. In contrast, the bacterial structure of the community enriched from PG was modified when sodium sulfate was replaced by PG as the sulfate source. This culture, which showed the poorest performance in the use of sulfate from PG, was mainly composed by SRB related to Desulfosporosinus genus. The present work provides new information regarding the phylogenetic characterization of anaerobic bacterial communities with the ability to use PG as sulfate donor, thus, contributing to improve the knowledge of microorganisms suitable to be used in PG bioremediation. Additionally, this paper demonstrates that an alternative to lactate and low-cost carbon source (wine wastes) can be used efficiently for that purpose
Status and Trends of Land Degradation and Restoration and Associated Changes in Biodiversity and Ecosystem Functions
A Complete Sequence and Transcriptomic Analyses of Date Palm (Phoenix dactylifera L.) Mitochondrial Genome
Based on next-generation sequencing data, we assembled the mitochondrial (mt) genome of date palm (Phoenix dactylifera L.) into a circular molecule of 715,001 bp in length. The mt genome of P. dactylifera encodes 38 proteins, 30 tRNAs, and 3 ribosomal RNAs, which constitute a gene content of 6.5% (46,770 bp) over the full length. The rest, 93.5% of the genome sequence, is comprised of cp (chloroplast)-derived (10.3% with respect to the whole genome length) and non-coding sequences. In the non-coding regions, there are 0.33% tandem and 2.3% long repeats. Our transcriptomic data from eight tissues (root, seed, bud, fruit, green leaf, yellow leaf, female flower, and male flower) showed higher gene expression levels in male flower, root, bud, and female flower, as compared to four other tissues. We identified 120 potential SNPs among three date palm cultivars (Khalas, Fahal, and Sukry), and successfully found seven SNPs in the coding sequences. A phylogenetic analysis, based on 22 conserved genes of 15 representative plant mitochondria, showed that P. dactylifera positions at the root of all sequenced monocot mt genomes. In addition, consistent with previous discoveries, there are three co-transcribed gene clusters–18S-5S rRNA, rps3-rpl16 and nad3-rps12–in P. dactylifera, which are highly conserved among all known mitochondrial genomes of angiosperms
Unique features of a global human ectoparasite identified through sequencing of the bed bug genome
The bed bug, Cimex lectularius, has re-established itself as a ubiquitous
human ectoparasite throughout much of the world during the past two decades.
This global resurgence is likely linked to increased international travel and
commerce in addition to widespread insecticide resistance. Analyses of the C.
lectularius sequenced genome (650 Mb) and 14,220 predicted protein-coding
genes provide a comprehensive representation of genes that are linked to
traumatic insemination, a reduced chemosensory repertoire of genes related to
obligate hematophagy, host–symbiont interactions, and several mechanisms of
insecticide resistance. In addition, we document the presence of multiple
putative lateral gene transfer events. Genome sequencing and annotation
establish a solid foundation for future research on mechanisms of insecticide
resistance, human–bed bug and symbiont–bed bug associations, and unique
features of bed bug biology that contribute to the unprecedented success of C.
lectularius as a human ectoparasite
The Complete Chloroplast Genome Sequence of Date Palm (Phoenix dactylifera L.)
BACKGROUND: Date palm (Phoenix dactylifera L.), a member of Arecaceae family, is one of the three major economically important woody palms--the two other palms being oil palm and coconut tree--and its fruit is a staple food among Middle East and North African nations, as well as many other tropical and subtropical regions. Here we report a complete sequence of the data palm chloroplast (cp) genome based on pyrosequencing. METHODOLOGY/PRINCIPAL FINDINGS: After extracting 369,022 cp sequencing reads from our whole-genome-shotgun data, we put together an assembly and validated it with intensive PCR-based verification, coupled with PCR product sequencing. The date palm cp genome is 158,462 bp in length and has a typical quadripartite structure of the large (LSC, 86,198 bp) and small single-copy (SSC, 17,712 bp) regions separated by a pair of inverted repeats (IRs, 27,276 bp). Similar to what has been found among most angiosperms, the date palm cp genome harbors 112 unique genes and 19 duplicated fragments in the IR regions. The junctions between LSC/IRs and SSC/IRs show different features of sequence expansion in evolution. We identified 78 SNPs as major intravarietal polymorphisms within the population of a specific cp genome, most of which were located in genes with vital functions. Based on RNA-sequencing data, we also found 18 polycistronic transcription units and three highly expression-biased genes--atpF, trnA-UGC, and rrn23. CONCLUSIONS: Unlike most monocots, date palm has a typical cp genome similar to that of tobacco--with little rearrangement and gene loss or gain. High-throughput sequencing technology facilitates the identification of intravarietal variations in cp genomes among different cultivars. Moreover, transcriptomic analysis of cp genes provides clues for uncovering regulatory mechanisms of transcription and translation in chloroplasts
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