Association of serum and follicular fluid leptin and ghrelin levels with in vitro fertilization success

 Objectives: The aim of this study was to evaluate the relationship between in vitro fertilization (IVF) cycle outcomes, serum and follicular fluid (FF) levels of leptin and ghrelin. Material and methods: Forty-four women who underwent intracytoplasmic sperm injection cycles (ICSI) were enrolled in the study. On the third day (D3) of the menstrual cycle, venous blood samples were drawn for serum measurements of leptin and ghrelin. The follicular fluid (FF) and the corresponding oocyte were obtained from a single dominant preovulatory follicle at the time of oocyte pick-up. The FF and D3 serum leptin and ghrelin concentrations were measured by enzyme-linked immunosorbent assay. The relationship between pregnancy rate and serum, follicular fluid levels of leptin and ghrelin were analyzed. Results: Of the 44 cases included, nineteen achieved clinical pregnancy (43.18%). Follicular fluid ghrelin levels were significantly lower in the pregnant group than non-pregnant group (p < 0.05) With respect to FF leptin, there was no statistically significant differences between the pregnant and non-pregnant women (p > 0.05). There was no statistically significant difference in D3 serum ghrelin between pregnant and non-pregnant groups (p > 0.05). However, D3 serum leptin levels were significantly lower in pregnant women than non-pregnant women (p < 0.05). Conclusions: Lower ghrelin levels in the follicular fluid were associated with higher pregnancy rates. Also, D3 serum leptin levels were inversely correlated with clinical pregnancy rates. These findings support the potential role of these molecules on IVF outcomes

Normoresponder hastalarda in-vitro fertilizasyon başarısızlığı sonrası protokol seçimi ne olmalı: Agonist mi, antagonist mi?

Amaç: Gonadotropin salgılatıcı hormon (GnRH) agonist ile downregüle edilmiş, başarısız bir in-vitro fertilizasyon (İVF) siklusunu takip eden tedavi siklusunda protokol tercihinin agonist veya antagonist olmasının gebelik sonuçları üzerine etkisinin değerlendirilmesi. Gereç ve Yöntemler: Bu bir retrospektif çalışmadır. Bir İVF ünitesinde 2002-2012 yılları arasında GnRH agonist protokol kullanılan ve başarısız bir İVF siklusu sonrasında bir yıl içinde tekrar İVF uygulanan 269 hasta çalışmaya dahil edilmiştir. Hastaların yaşı, FSH değerleri, antral follikül sayıları, indüksiyon süreleri, toplanılan oosit sayısı, transfer edilen embriyo sayısı ve transfer günleri, klinik ve devam eden gebelik oranları her iki tedavi siklusu için değerlendirilmiştir. Bulgular: Normoresponder hastalar ikinci siklusta agonist ya da antagonist protokol seçimine göre ikiye ayrılmış ve ardışık iki İVF sikluslarının sonuçları karşılaştırılmıştır. Uygulanan gonadotropin dozu, indüksiyon süresi, elde edilen oosit sayısı, embriyo transfer günü ve sayısı açısından her iki grup arasında istatistiksel olarak anlamlı bir fark saptanmamıştır (p>0,05). Ayrıca fertilizasyon, klinik ve devam eden gebelik oranları açısından her iki grup benzer oranlara sahiptir. Sonuç: Normoresponder hastalarda İVF başarısızlığı sonrasında antagonist tedavi seçimi agonist protokoller kadar etkilidir. J Turk Soc Obstet Gynecol 2014;4:198-202Objective: Evaluation of the impact of agonist or antagonist protocol selection on pregnancy outcomes after failure of in-vitro fertilization (IVF) treatment cycles which were down regulated with Gonadotropin Releasing Hormone (GnRH) agonist. Materials and Methods: This was a retrospective study. Two hundred and sixty nine patients who were treated with GnRH agonist protocol between years 2002-2012 at an IVF unit and underwent a second attempt following one year period after failure of IVF enrolled in the study. Age, basal FSH levels, antral follicle counts, duration of induction, the number of yielded oocytes, the number of transferred embryos and the transfer days, clinical and ongoing pregnancy rates were evaluated for each treatment cycle. Results: Normoresponder patients were separated into two groups according to the agonist or antagonist protocol selection at the second attempt and the results of two consequent IVF cycles were compared. There were no statistically significant difference between the groups for the dosage of administered gonadotropin, duration of induction, the count of yielded oocytes, the day and the number of transferred embryos (p>0.05). Furthermore the fertilization rate, clinical and ongoing pregnancy rates were similar in two groups. Conclusion: The selection of antagonist treatment is effective as agonist protocols at normoresponder patients after failure of IVF. J Turk Soc Obstet Gynecol 2014;4:198-20

Association of serum and follicular fluid leptin and ghrelin levels with in vitro fertilization success

Dirican, Enver/0000-0001-6230-1229; Gode, Funda/0000-0002-9371-6942; Dirican, Enver/0000-0001-6230-1229WOS: 000411999200002PubMed: 29949336Objectives: The aim of this study was to evaluate the relationship between in vitro fertilization (IVF) cycle outcomes, serum and follicular fluid (FF) levels of leptin and ghrelin. Material and methods: Forty-four women who underwent intracytoplasmic sperm injection cycles (ICSI) were enrolled in the study. On the third day (D3) of the menstrual cycle, venous blood samples were drawn for serum measurements of leptin and ghrelin. The follicular fluid (FF) and the corresponding oocyte were obtained from a single dominant preovulatory follicle at the time of oocyte pick-up. The FF and D3 serum leptin and ghrelin concentrations were measured by enzyme-linked immunosorbent assay. The relationship between pregnancy rate and serum, follicular fluid levels of leptin and ghrelin were analyzed. Results: Of the 44 cases included, nineteen achieved clinical pregnancy (43.18%). Follicular fluid ghrelin levels were significantly lower in the pregnant group than non-pregnant group (p 0.05). There was no statistically significant difference in D3 serum ghrelin between pregnant and non-pregnant groups (p > 0.05). However, D3 serum leptin levels were significantly lower in pregnant women than non-pregnant women (p < 0.05). Conclusions: Lower ghrelin levels in the follicular fluid were associated with higher pregnancy rates. Also, D3 serum leptin levels were inversely correlated with clinical pregnancy rates. These findings support the potential role of these molecules on IVF outcomes

Association of serum and follicular fluid leptin and ghrelin levels with in vitro fertilization success

Objectives: The aim of this study was to evaluate the relationship between in vitro fertilization (IVF) cycle outcomes, serum and follicular fluid (FF) levels of leptin and ghrelin

Association of serum and follicular fluid leptin and ghrelin levels with in vitro fertilization success

Objectives: The aim of this study was to evaluate the relationship between in vitro fertilization (IVF) cycle outcomes, serum and follicular fluid (FF) levels of leptin and ghrelin