The role of corifollitropin alfa in controlled ovarian stimulation for IVF in combination with GnRH antagonist

Corifollitropin alfa is a synthetic recombinant follicle-stimulating hormone (rFSH) molecule containing a hybrid beta subunit, which provides a plasma half-life of ∼65 hours while maintaining its pharmocodynamic activity. A single injection of corifollitropin alfa can replace daily FSH injections for the first week of ovarian stimulation for in vitro fertilization. Stimulation can be continued with daily FSH injections if the need arises. To date, more than 2500 anticipated normoresponder women have participated in clinical trials with corifollitropin alfa. It is noteworthy that one-third of women did not require additional gonadotropin injections and reached human chorionic gonadotropin criterion on day 8. The optimal corifollitropin dose has been calculated to be 100 μg for women with a body weight ≤60 kg and 150 μg for women with a body weight >60 kg, respectively. Combination of corifollitropin with daily gonadotropin-releasing hormone antagonist injections starting on stimulation day 5 seems to yield similar or significantly higher numbers of oocytes and good quality embryos, as well as similar ongoing pregnancy rates compared with women stimulated with daily rFSH injections. Stimulation characteristics, embryology, and clinical outcomes seem consistent with repeated corifollitropin-stimulated assisted reproductive technologies cycles. Multiple pregnancy or ovarian hyperstimulation syndrome rates with corifollitropin were not increased over daily FSH regimen. The corifollitropin alfa molecule does not seem to be immunogenic and does not induce neutralizing antibody formation. Drug hypersensitivity and injection-site reactions are not increased. Incidence and nature of adverse events and serious adverse events are similar to daily FSH injections. Current trials do not provide information regarding use of corifollitropin alfa in anticipated hyper- and poor responders to gonadotropin stimulation. Although corifollitropin alfa is unlikely to be teratogenic, at the moment data on congenital malformations is missing

Effects of various extremely low frequency magnetic fields on the free radical processes, natural antioxidant system and respiratory burst system activities in the heart and liver tissues

326-331Magnetic fields (MFs) can affect biological systems by increasing the release of free radicals that are able to alter cell defense systems and breakdown tissue homeostasis. In the present study, the effects of extremely low frequency (ELF) electromagnetic fields (EMF) were investigated on free radical levels, natural antioxidant systems and respiratory burst system activities in heart and liver tissues of guinea pigs exposed to 50 Hz MFs of 1, 2 and 3 mT for 4 h/day and 8 h/day for 5 days by measuring malondialdehyde (MDA), nitric oxide (NO), glutathione (GSH) levels and myeloperoxidase (MPO) activity. A total of sixty-two male guinea pigs, 10-12 weeks old were studied in seven groups as control and exposure groups: Group I (control), II (1 mT, 4 h/day), III (1 mT, 8 h/day), IV (2 mT, 4 h/day), V (2 mT, 8 h/day), VI (3 mT, 4 h/day), and VII (3 mT, 8 h/day). Controls were kept under the same conditions without any exposure to MF. MDA levels increased in liver in groups II and IV, but decreased in group VII for both liver and heart tissues. NOx levels declined in heart in groups II and III and in liver in groups III, V, and VI, but increased in liver in group VII. GSH levels increased in heart in groups II, IV, V, and in liver in groups V and VI and VI, but decreased in groups II and IV in liver. MPO activity decreased in liver in groups III, IV, VI and VII with respect to controls and in heart tissues in groups II, III and IV; however, there was a significant increase MPO activity in heart in group VII. From the results, it can be concluded that the intensity and exposure duration of MFs are among the effective conditions on the formation of free radicals and behaviour of antioxidant enzymes

Formulation of ELF magnetic fields' effects on malondialdehyde level and myeloperoxidase activity in kidney using genetic programming

In vivo exposure effects of electromagnetic fields (EMFs) on various tissues of experiment animals have been investigated. In this sense, modeling and formulation of these biological effects have been of significant importance. In this study extremely low frequency (ELF) EMFs effects on malondialdehyde (MDA) level and myeloperoxidase (MPO) activity in kidney of guinea pigs exposed to 50 Hz magnetic fields of 1 mT, 2 mT and 3 mT have been presented. It has been planned to determine whether genetic programming (GP) is appropriate to analyze and formulate these biological effects. Consequently, it has been observed that GP can be effectively used to model MDA level and MPO activity. The performances of prediction of the proposed GP formulation versus actual experimental values are found to be quite satisfactory in terms of standard deviation and correlation coefficient. It is concluded that the GP application serves to form a database for the researchers in this field, without exposing tissues to EMF and without using too many guinea pigs. (c) 2006 Elsevier Ireland Ltd. All rights reserved

Formulation of low intensity direct current effects on wound healing of skin using genetic programming

This study has two main purposes: Firstly.. the effect of electrical stimulation was studied on experimental wound healing of skin. It investigates whether a triggering was possible on collagen synthesis in wound healing before the 3rd day Of wounding, via measurements of hydroxyproline, a measure of collagen concentration in the wound tissue. Wound tissue hydroxyproline contents were determined with the effects of two different electric current intensities of. 200 mu A and 400 mu A at 48 and 72 hours after wounding by Woessner's Method. For both of the current intensities, statistically significant increases were found in hydroxyproline contents of current applied wounds with respect to their controls for 72 hours whereas decreased hydroxyproline content was found for 400 mu A of current intensities at 48 hours. Secondly, it was aimed to model and formulate how electric current effects wound healing using Genetic Programming (GP) based on experimental results. The accuracy of the proposed GP formulation is defined quantiatively by standard deviation (SD=0.39) and correlation coefficient (R=0.85), which is found to be high

Histerektominin seksüel fonksiyonlar üzerine olan etkisi

Amaç: Histerektominin seksüel fonksiyonlar üzerine olan etkisi tartışmalıdır. Histerektomi için endikasyon alan hastalar mevcut klinik hastalıklarından dolayı belirgin olarak daha önceki fiziksel, ruhsal ve seksüel statülerini bozan bir stress ile karşı karşıyadır. Operasyon sonrası uterin patolojiye bağlı olumsuz etkilerin sona ermesi bek­lenir. Bu çalışmada histerektomi sonrası premenopozal kadınlarda seksüel fonksiyonun durumu değerlendirildi Çalışma düzeni: Prospektif kontrollü çalışma Gereç ve yöntem: Yaşları 32-40 arasında değişen benign uterin hastalıklar nedeniyle en az 2 yıl önce överleri korunarak histerektomi uygulanmış ve överleri fonkiyonel olarak aktif 40 premenopozal kadın çalışmaya alındı. Aynı yaş grubunda (30-40 yaş) seksüel yaşamlanyla ilgili şikayeti bulunmayan sağlıklı premenopozal 40 kadın ile kontrol grubu oluşturuldu. Çalışmaya alman kadınların rutin tetkikleri yapıldı ve menopozal durumları serum FSH ve Östrodiol seviyeleri bakılarak teyid edildi. Tüm olgulara "Index of female sexual function" (IFSF) anketi uygulanarak seksüel istek, seksüel uyarım, lubrikasyon, orgazm, seksüel tatmin ve seksüel ağrı parametrelerinin skorlaması yapıldı. Bulgular: Histerektomize kadınlarda yaş ortalaması 35±5.3 kontrol grubunda ise 36±4.6 yıl olarak hesaplandı. Çalışmaya alınan bütün kadınlarda premenopozal dönem FSH ve Östradiol seviyeleri tespit edildi. En sık histerekto­mi nedeninin uterin myomlar olduğu görüldü. Histerek­tomize kadınlarda seksüel istek, lubrikasyon, orgazm ve seksüel ağrı skorları kontrol grubu ile karşılaştırıldığında anlamlı oranda düşük bulundu. Sonuç: Histerektomi sırasında vajinayı, serviksi ve uterusu besleyen küçük arter dallarının ve bu dokuları innerve eden sinir liflerinin travmatize edildiğini ve seksüel disfonksiyon için potansiyel oluşturduğunu düşünüyoruz. Radikal prostat cerrahisinde sinir koruyucu teknik ile erek-til fonksiyon devamlılığı sağlandığı gibi kadınlar için de pelvik cerrahilerde seksüel sağlığın sürdürülmesi için sinir­leri koruyucu cerrahi teknikler gündeme getirilmelidir

Prevention of adhesion formation following ovarian surgery in a standardized animal model: comparative study of Interceed and double layer Surgicell.

Aim: Comparison of antiadhesive performances of double layer Surgicell and single layer Interceed following ovarian surgery in a rabbit model

Pentylenetetrazol-induced seizures are not altered by pre- or post-drug exposure to a 50 Hz magnetic field

WOS: 000246068200003PubMed: 17575950Purpose: To investigate whether pre- and post-drug magnetic field (MF) exposure of 50 Hz, 0.2 mT has any significant effect on pentylenetetrazol (PTZ)-induced seizures in mice. Material and methods: MF was generated by a pair of Helmholtz coils. Seizures were induced by PTZ injection intraperitoneally (i.p.) at a dose of 60 mg/kg. A total of 48 locally bred adult female mice 25 - 35 g in weight were used. Latency to seizure, total seizure duration, and mortality were recorded for each mouse. Results: Neither pre- nor post-drug exposure to a 50 Hz, 0.2 mT MF was found to have any effect on PTZ-induced epileptic seizures or mortality rates in mice. Conclusion: The present study failed to provide any support for a therapeutic potential of a 50 Hz, 0.2 mT MF for epilepsy