Kontinuirani postupak bistrenja soka od obične žutike pomoću pektinaze imobilizirane oksidiranim polisaharidima

Research background. Barberry juice is a rich source of bioactive compounds and shows different health properties such as antioxidant and anticancer activities. Clarification, as the removal process of suspended material, is an important step in the production of fruit juice due to its significant effect on the appearance, flavour and commercialisation of juice. Pectinase is the most important enzyme applied in juice clarification that breaks down the pectin polymer structure and reduces the undesirable turbidity. Pectinase immobilisation is a way to overcome free enzyme drawbacks such as instability, high cost, the difficulty of recovery and recyclability. Also, continuous clarification process which is highly preferred in fruit juice industry is not possible without enzyme immobilisation. Experimental approach. Pectinase enzymes were immobilised on the functionalised glass beads (glass bead with (3-aminopropyl)triethoxysilane) by glutaraldehyde, polyaldehyde derivatives of pullulan and kefiran and the barberry juice was clarified in the batch and continuous processes in a packed bed reactor (PBR). Also, the effect of clarification on the physicochemical and antioxidant properties of the barberry juice samples was evaluated. Results and conclusions. The optimum conditions for clarification in the PBR were: flow rate 0.5 mL/min, temperature 50 °C and treatment time 63 min. Clarification led to a decrease in turbidity, pH, total soluble solid content, viscosity, total phenolic content and antioxidant activity of the juice samples. Also, this process increased the clarity, acidity, reducing sugar concentration and the lightness parameter of the barberry juice. The greatest effect of clarification on the studied properties of barberry juice was related to the pectinase immobilised by the polyaldehyde of kefiran in the continuous process and both new cross-linkers (polyaldehyde derivatives of pullulan and kefiran) immobilised the enzyme better than the common cross-linker (glutaraldehyde). Novelty and scientific contribution. For the first time, barberry juice was clarified with pectinase immobilised by polyaldehyde derivatives of pullulan and kefiran and the obtained results showed that the pectinase immobilisation by these new cross-linkers was much more efficient than by the glutaraldehyde as a common cross-linker. These findings can be of use for an industrialised production of fruit juices.Pozadina istraživanja. Sok od obične žutike bogat je bioaktivnim spojevima te ima razna ljekovita svojstva, poput antioksidacijskih i antikancerogenih. Bistrenje je postupak uklanjanja materijala iz suspenzije i važan je korak u proizvodnji sokova jer bitno utječe na izgled, okus i ekonomsku isplativost soka. Pektinaza je najvažniji enzim koji se koristi za bistrenje sokova, a razgrađuje polimernu strukturu pektina i time smanjuje zamućenost soka. Imobilizacijom pektinaze uklanjaju se nedostaci slobodnog enzima, kao što su nestabilnost, visoka cijena, malo iskorištenje i slaba ponovna iskoristivost. Osim toga, kontinuirani postupak bistrenja, koji se najviše koristi u proizvodnji sokova, nije moguće provesti bez imobilizacije enzima. Eksperimentalni pristup. Pektinaza je imobilizirana pomoću glutaraldehida te polialdehidnih derivata pululana i kefirana na staklenim kuglicama prevučenim (3-aminopropil)trietoksisilanom, a sok od obične žutike bistren je šaržnim ili kontinuiranim postupkom u reaktoru s nasutim slojem nosača. Osim toga, ispitana su fizikalno-kemijska i antioksidacijska svojstva soka. Rezultati i zaključci. Optimalni uvjeti za bistrenje soka u reaktoru s nasutim slojem nosača bili su: protok 0,5 mL/min, temperatura 50 °C i trajanje postupka 63 min. Postupkom bistrenja smanjila se zamućenost soka, snizila njegova pH-vrijednost i smanjili ukupan udjel topljive tvari, viskoznost, ukupan udjel fenola te antioksidacijska aktivnost. Osim toga, povećali su se bistroća, kiselost, koncentracija reducirajućih šećera te parametri boje soka. Svojstva soka najviše su se poboljšala bistrenjem pomoću pektinaze imobilizirane polialdehidom kefirana u kontinuiranom postupku. Oba derivata polialdehida, pululan i kefiran, imobilizirali su enzim bolje od uobičajeno korištenog glutaraldehida. Novina i znanstveni doprinos. U ovom je radu po prvi put proveden postupak bistrenja soka od obične žutike pomoću pektinaze imobilizirane derivatima polialdehida pululana i kefirana. Dobiveni rezultati pokazuju da je ovako imobilizirana pektinaza bitno učinkovitija od one imobilizirane pomoću glutaraldehida, te se može upotrijebiti u industrijskoj proizvodnji voćnih sokova

Housing development and the smart city: a case study of Tehran, Iran

Iran has one of the oldest civilizations in the world, and many elements of today’s urban planning and design have their origins in the country. However, mass country-city migration from the 1960s onwards brought enormous challenges for the country’s main cities in the provision of adequate housing and associated services, resulting in a range of sub-standard housing solutions, particularly in Tehran, the capital city. At the same time, and notably in the past decade, Iran’s main cities have had significant involvement in the smart city movement. The Smart Tehran Program is currently underway, attempting to transition the capital towards a smart city by 2025. This study adopts a qualitative, inductive approach based on secondary sources and interview evidence to explore the current housing problems in Tehran and their relationship with the Smart Tehran Program. It explores how housing has evolved in Tehran and identifies key aspects of the current provision, and then assesses the main components of the Smart Tehran Program and their potential contribution to remedying the housing problems in the city. The article concludes that although housing related issues are at least being raised via the new smart city technology infrastructure, any meaningful change in housing provision is hampered by the over centralized and bureaucratic political system, an out of date planning process, lack of integration of planning and housing initiatives, and the limited scope for real citizen participation

The effect of regular resistance exercise, vitamin D, and calcium supplements on the gastrocnemius muscle in rats in the post-menopausal period: An experimental study

Background: Menopause is the natural termination of menstruation which affects the quality and important aspects of women’s life. Objective: To evaluate the effect of regular resistance training (Ex) with vitamin D (Vit. D) and calcium (Ca) supplements in the postmenopausal period on muscle tissue in rats. Materials and Methods: In this experimental study, 72 female Wistar rats (8-10-wk old) were randomly divided into control, placebo, Vit. D, Ca, Ex, Ca + Vit. D, Ex + Ca, Ex + Vit. D, and Ex + Ca + Vit. D groups. Control and placebo groups were fed with a standard diet and sesame oil, respectively. Two months after the ovariectomy, Ex, Ca (35 mg/kg), and Vit. D (10000 IU) were administered in all groups except the control. The number of muscle and inflammatory cells, fiber diameter, endomysium thickness, and degenerative collagen fiber area were assessed through hematoxylin-eosin staining. Results: Muscle cell number was increased in the Ex + Vit. D + Ca, Vit. D + Ex, and Vit. D groups compared to the control group; also, inflammatory cell number showed significant increase in the Ex + Vit. D + Ca (12 ± 5.46), Vit. D + Ex (14 ± 3.25), Ex (13 ± 4.08), Vit. D (11 ± 3.26), Ca + Vit. D (10 ± 1.01), and Ca + Ex (9 ± 2.87) groups. Muscle fiber diameter in the Ex + Vit. D + Ca and Vit. D + Ex groups was higher than the other groups. Endomysium thickness was significantly decreased in the Ex + Vit. D + Ca and Vit. D + Ex groups compared to the control and placebo groups (p < 0.001). Degenerative collagen fiber area showed a significant increase in the Ex + Vit. D + Ca and Vit. D + Ex groups (p ≤ 0.001) comparison with the control group. Conclusion: Regular resistance exercise, Vit. D, and Ca supplements can improve muscle morphological features in the postmenopausal period. Key words: Menopause, Muscle, Vitamin D, Calcium, Exercise

Hemodialysis Patients and Nurses’ Perspective: What Is a Qualified Nursing Care?

Background: With the high prevalence of chronic diseases such as chronic kidney disease (CKD) and consequently, the increasing number of hemodialysis (HD) patients, providing high-quality care has become a health concern in many countries. Therefore, the present study aimed to explore the concept of “qualified care” from HD patients’ and nurses’ points of view. Methods: This qualitative study was conducted using a content analysis approach. A total of 48 patients and nurses in the HD wards were selected through purposive and snowball sampling methods. Sampling continued until data saturation. Finally, a total of 54 interviews were conducted with the participants. The interviews were recorded with the participants’ permission and analyzed using Graneheim and Lundman approach. Results: In this study, 16.7% of the nurses were male, and their mean age was 35.4 ± 7.47 years. Moreover, 50% of the HD patients were male, and their mean age was 53.1 ± 8.6 years. The results of the analysis of the interviews in response to the main question were classified into four main themes including (a) physical care, (b) psychological care, (c) ethical care, and (d) extensive skills and knowledge of the nurse. Conclusion: From the patients’ and nurses’ perspective, empathy and companionship in sessions, fulfilling daily needs, social support, and high-quality dialysis are the components of the concept of care. Therefore, these concepts should be considered in the care programs for HD patients designed by the healthcare team

Evaluation and Comparison of Choroidal Thickness in Patients with Behçet Disease with versus without Ocular Involvement

Purpose: To assess the subfoveal choroidal thickness (SFCT) in patients with Behçet disease (BD) and compare the SFCT in patients with and without ocular BD (OBD) and between patients with active and quiescent phases of the Behçet’s posterior uveitis. Method: This prospective cross-sectional study was conducted on patients with BD (n = 51) between October 2016 and October 2018. Complete ocular examinations including slit lamp biomicroscopy and fundus examination with dilated pupils were performed for all patients. The SFCT values were compared between patients with and without OBD. Enhanced depth imaging optical coherence tomography (EDI–OCT) was done to measure the SFCT, and wide field fundus fluorescein angiography (WF–FAG) was performed to evaluate the ocular involvement and determine the active or quiescent phases of the Behçet’s posterior uveitis. The correlation between the changes of SFCT and the WF-FAG scores was assessed. Results: One hundred and two eyes of 51 patients with BD, aged 29 to 52 years were studied. Of these, 23 patients were male. The mean age ± standard deviation in patients with OBD and patients without ocular involvement was 38.71 ± 7.8 and 36.22 ± 10.59 years (P = 0.259) respectively. The mean SFCT in patients with OBD was significantly greater than in patients without OBD (364.17 ± 93.34 vs 320.43 ± 56.70 μm; P = 0.008). The difference of mean SFCT between the active compared to quiescent phase was not statistically significant when only WF-FAG criteria were considered for activity (368.12 ± 104.591 vs 354.57 ± 58.701 μm, P = 0.579). However, when the disease activity was considered based on both WF-FAG and ocular exam findings, SFCT in the active group was higher than the inactive group (393.04 ± 94.88 vs 351.65 ± 58.63 μm, P = 0.060). This difference did not reach statistical significance, but it was clinically relevant. Conclusion: Choroidal thickness was significantly increased in BD patients with ocular involvement; therefore, EDI-OCT could be a noninvasive test for evaluation of ocular involvement in patients with BD. The increased SFCT was not an indicative of activity in OBD; however, it could predict possible ocular involvement throughout the disease course

Isolated Intraconal Meningioma

Purpose: To report a rare case of isolated intraconal meningioma. Case Report: A 24-year-old woman presented with painless proptosis in her left eye which started and progressed during her pregnancy about 10 months ago. Hertel exophthalomometry revealed anterior displacement of the globe with 4 mm of proptosis which was remarkable. Magnetic resonance imaging (MRI) demonstrated an intraconal circumscribed oval-shaped mass with hypointense signals on T1- weighted images and hyperintense signals on T2-weighted images, mimicking cavernous hemangioma. This mass, however, was free of any connections to optic nerve or bones. Due to the imaging characteristics, more prevalent diagnoses like cavernous hemangioma were placed on the top of the differential diagnoses list. However, during the surgical excision, the tumor’s consistency and gross features were not compatible with cavernous hemangioma. The pathologic findings instead determined meningotheliomatous meningioma, a very rare condition, which was far from our expectations prior to the surgery. Conclusion: Ectopic orbital meningiomas are rare tumors that are not easily diagnosed without postoperative histopathology. Despite its low prevalence, they should be considered in the differential diagnosis list of intraconal masses with hypointense signals on T1-weighted images and hyperintense signals on T2- weighted images

Coenzyme Q10 and Its Therapeutic Potencies Against COVID-19 and Other Similar Infections: A Molecular Review

Purpose: New lethal coronavirus disease 2019 (COVID-19), currently, has been converted to a disastrous pandemic worldwide. As there has been found no definitive treatment for the infection in this review we focused on molecular aspects of coenzyme Q10 (CoQ10) and possible therapeutic potencies of CoQ10 against COVID-19 and similar infections. Methods: This is a narrative review in which we used some authentic resources including PubMed, ISI, Scopus, Science Direct, Cochrane, and some preprint databases, the molecular aspects of CoQ10 effects, regarding to the COVID-19 pathogenesis, have been analyzed and discussed. Results: CoQ10 is an essential cofactor in the electron transport chain of the phosphorylative oxidation system. It is a powerful lipophilic antioxidant, anti-apoptotic, immunomodulatory and anti-inflammatory supplement which has been tested for the management and prevention of a variety of diseases particularly diseases with inflammatory pathogenesis. CoQ10 is a strong anti-inflammatory agent which can reduce tumor necrosis factor-α (TNF-α), interleukin (IL)- 6, C-reactive protein (CRP), and other inflammatory cytokines. The cardio-protective role of CoQ10 in improving viral myocarditis and drug induced cardiotoxicity has been determined in different studies. CoQ10 could also improve the interference in the RAS system caused by COVID-19 through exerting anti-Angiotensin II effects and decreasing oxidative stress. CoQ10 passes easily through blood–brain barrier (BBB). As a neuroprotective agent CoQ10 can reduce oxidative stress and modulate the immunologic reactions. These properties may help to reduce CNS inflammation and prevent BBB damage and neuronal apoptosis in COVID-19 patients. Conclusion: CoQ10 supplementation may prevent the COVID-19-induced morbidities with a potential protective role against the deleterious consequences of the disease, further clinical evaluations are encouraged

The emerging role of miR-20b in human cancer and other disorders: Pathophysiology and therapeutic implications

miR-20b is a microRNA with diverse and somehow contradictory roles in the pathogenesis of human disorders, especially cancers. It has been known to be a tumor suppressor in colon cancer, renal cell carcinoma, prostate cancer, osteosarcoma and papillary thyroid cancer. In lung cancer and breast cancers, both tumor suppressor and oncogenic effects have been identified for this miRNA. Finally, in T cell leukemia, hepatocellular carcinoma, esophageal squamous cell carcinoma and cervical and gastric cancers, miR-20b is regarded as an oncogenic miRNA. In several types of cancer, dysregulation of miR-20b has been recognized as a predictive marker for patients’ survival. Dysregulation of miR-20b has also been recognized in Alzheimer’s disease, diabetic retinopathy, myocardial ischemia/infarction, chronic hepatitis B and multiple sclerosis. In the current review, we have summarized the miR-20b targets and related cellular processes. We have also provided a review of participation of this miRNA in different human disorders

Psychometrics properties of the Iranian version of fertility quality of life tool: a cross- sectional study

Background: Clinical measurement of quality of life for assessing reproductive problems should be considered as a standard investigation at the initial and continuing medical consultations with infertile people. Objective: The purpose of this study was comprehensive testing the psychometric properties of the Iranian version of fertility quality of life (FertiQol) as its use could be considerable due to the prevalence of infertility in Iran and the importance of evaluation of QoL in patients with infertility. Method and material: This was a psychometric properties study of 300 women referred to infertility clinic, Hormozgan, Iran. After linguistic validation of the Iranian version of MPCOSQ, a semi-structured interview was conducted to assess face validity. Consequently exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) were performed to indicate the scale constructs. Discriminant validity was assessed using the known groups comparison. Convergent validity was evaluated by assessing the correlation between similar content on the 12-Item Short Form Health Survey, Hospital Anxiety and Depression Scale and FertiQol. In addition, reliability analysis was carried out with internal consistency. Results: The reliability of the Iranian version of the FertiQol questionnaire was satisfactory in all dimensions (Cronbach’s alpha ranging from 0.77 to 0.83 ). Six factors (emotional, mind/body, relational, social, environmental and tolerability) were extracted from the results of EFA with factor loading of the more than 0.3. Discrimination validity showed that FertiQol can differentiate between female patients with differing duration of infertility and number of children; this indicates favorable discrimination validity. Moreover, the results of convergent validity showed a favorable correlation between the related dimensions of 12-Item Short Form Health Survey (correlation ranging from 0.43 to 0.68 ), Hospital Anxiety and Depression Scale(correlation ranging from 0.47 to 0.52 ) and FertiQol. Conclusion: The Iranian version of FertiQol is valid and reliable for assessing infertility problems and the effects of treatment on quality of life of infertile patients referred for diagnosis and treatment at an Iranian infertility clinic. The clinical use of this measure is recommended for Iranian infertility clinics