Using an ensemble modeling to predict the potential distribution and habitat suitability of caracal (Caracal caracal) in southwestern Iran

Anthropogenic activities and climate change are degrading wildlife habitats, making it crucial to assess suitability of remaining habitat for effective conservation and management of threatened wildlife. This study aimed to determine the variables influencing habitat selection and spatial distribution of caracal (Caracal caracal) in southwestern Iran from 2019 to 2020. Ensembles of Small Models (ESM) were used based on eight species distribution models that included a combination of statistical and machine learning methods (GLM, CTA, FDA, GBM, ANN, MARS, RF, and MaxEnt). The outputs of two ensemble models were hybridized by simple averaging. In ESM 1, distance to water resources was used as a representative of environmental variables and in ESM 2, annual precipitation was used as a representative of bioclimatic variables. In both ESMs, these variables were combined with ecological variables such as the distribution of wild goat (Capra aegagrus) and livestock. The average importance of the model variables for ESM 1 and ESM 2 showed that the distribution of wild goat and livestock were the most important factors in predicting suitable habitat for caracals, respectively. Habitat suitability was higher close to the distribution of wild goat and livestock, near water sources and during periods of higher precipitation. Probabilistic predictions of caracal presence based on the hybridization of ESM 1 and ESM 2 suggest that only 1.3 % (206 ha) of the study area was highly suitable habitat, while 58.4 % (9570 ha) was unsuitable. Based on the ensemble of binary presence-absence maps, caracals were certainly absent in 76.2 % (12,490 ha) of the area, while their presence was uncertain in 21.6 % (3536 ha). The presence of caracals was certain in 2.2 % (363 ha) of the study area. Awareness of the habitat and distribution of caracal can help to make better decisions towards conservation and management and also reduce its conflict with humans

Effect of Using Micronutrients Elements on Physiological Performance Indicators in the Calendula Officinalist L

Background and Objective: Calendula officinalist is a grass, one annual plant and race of Asteraceae. This plant is a valuable medicinal plant that In addition to the medicinal properties, it has ornamental aspects. Methods: To study the effects of micronutrient elements on the physiological properties of Calendula officinalist in the term of a completely randomized design with three replications in 1390 was conducted at the research farm of Islamic Azad University of shahrkord. Treatments of iron and zinc in three levels [0, 2 and 4 per thousand] on Calendula officinalist plants studied and evaluated. During this test Calendula officinalist 2 times, at 4 to 6 leaf stage and early flowering, by iron and zinc were sprayed. Results and discussion: Based on results of One-way ANOVA test in this experiment, Concentration of 2 per thousand of Fe and Zn had the most effect on Calendula officinalist. Practical / Industrial advice: According to the results of the study, it is recommended that more study should be done on other micronutrients elements of different concentrations of this experiment in the effective ingredients of medicinal plants

The Effect of Interactive Content and Gamification on the Learning Rate and Motivation of Students in the Pediatric Nursing Virtual Internship Course

Introduction: The learning styles of students have changed in today's world and they need new teaching methods. In a situation like the spread of the Coronavirus, the use of virtual training such as gamification has flourished. Therefore, this study was conducted with the aim of investigating the effect of interactive content and gamification on the level of learning and students' motivation in the integrated course of pediatric nursing virtual internship. Materials and Methods: This semi-experimental study was conducted on 49 sixth-semester nursing students of Rafsanjan University of Medical Sciences. The level of students' learning was assessed using a researcher-made questionnaire before and 10 days after the intervention. The motivation level was measured after the intervention by the standard educational materials motivational questionnaire (IMMS). This practical unit encompassed a 10-day internship in pediatric nursing. Each group received practical training and virtual assignments for 5 days, which simultaneously covered other internship days, in the pediatric department . Data were analyzed using SPSS version 18 software. Results: Based on the results, there was a statistically significant difference between the students' average learning scores before and after the implementation of gamification. The average post-test scores were higher than the pre-test scores, with a significant difference (p < 0.05). The average scores and standard deviation of the sum of the standard motivational questionnaire components were108±13 after the intervention; which indicates a moderate level of motivation created by this intervention. Conclusion: The results of the present study indicated the positive effect of gamification on students' motivation and learning. Therefore, it is recommended to use this method to facilitate students' learning

Antibiotic use during the first 6 months of COVID-19 pandemic in Iran : a large-scale multi-centre study

WHAT IS KNOWN AND OBJECTIVE: Although antibiotics are ineffective against viral infections, epidemiological studies have revealed that the COVID‐19 pandemic resulted in the overuse of antibiotics and disruption of antimicrobial stewardship programmes. We investigated the pattern of antibiotic use during the first 6 months of the COVID‐19 pandemic in Iran. METHODS: A multi‐centre retrospective study was designed to investigate the use of 16 broad‐spectrum antibiotics in 12 medical centres. The rate of antibiotic use was calculated and reported based on the Defined Daily Dose (DDD) per 100 hospital bed‐days. The bacterial co‐infection rate was also reported. RESULTS AND DISCUSSION: Totally, 43,791 hospitalized COVID‐19 patients were recruited in this study. It was found that 121.6 DDD of antibiotics were used per 100 hospital bed‐days, which estimated that each patient received approximately 1.21 DDDs of antibiotics every day. However, the bacterial co‐infections were detected only in 14.4% of the cases. A direct correlation was observed between the rate of antibiotic use and mortality (r[142] = 0.237, p = 0.004). The rate of antibiotic consumption was not significantly different between the ICU and non‐ICU settings (p = 0.15). WHAT IS NEW AND CONCLUSION: In this study, widespread antibiotic use was detected in the absence of the confirmed bacterial coinfection in COVID‐19 patients. This over‐consumption of broad‐spectrum antibiotics may be associated with increased mortality in hospitalized COVID‐19 patients, which can be an alarming finding

Dronedarone, amiodarone and other antiarrhythmic drugs, and acute liver injuries: a case-referent study

International audienceBACKGROUND: Spontaneous reports of acute liver injuries (ALI) in patients taking dronedarone triggered an EMAalert in 2011. This study aimed to assess the risk of ALI for class III antiarrhythmic drugs controlling for the useof other potential ALI-inducing drugs.METHODS: Between 2010 and 2014, consecutive ALI cases (≥50 years-old) were identified across Germany. ALIwas defined as a new increase in at least one of the transaminases≥3 times the upper limit of normal (ULN)or≥2 ULN if alkaline phosphatase, with (“definite”case) or without (“biochemical”case) suggestive signs/symp-toms of ALI, excluding other liver diseases. Recruited community controls were matched to cases on gender, ageand inclusion date. Exposure to antiarrhythmic drugs and co-medication up to 2 years before ALI onset was in-formed by patients and confirmed by physicians' prescriptions. Adjusted Odds Ratios (aOR) were obtained fromconditional multivariable logistic regressions, adjusted for a multivariate disease risk score and co-medication.RESULTS: 252 cases and 1081 matched controls were included (59.1% females; mean age: 64 years). Exposure toclass III antiarrhythmic drugs was 4.0% in cases and 1.5% in controls, aOR = 3.6 (95% CI: 1.6–8.4). Associationswith exposure to dronedarone and amiodarone were respectively 3.1 (95% CI: 0.7–14. 8) and 5.90 (1.7–20.0).Restricting the analysis to definite or severe ALI cases did not change these results.CONCLUSIONS: Class III antiarrhythmic drugs were associated with ALI, amiodarone displaying the highest risk, andresults were robust to case definitions. Continued vigilance is needed for patients taking these drug

SD1000: High Sustained Viral Response Rate in 1361 Patients With Hepatitis C Genotypes 1, 2, 3, and 4 Using a Low-cost, Fixed-dose Combination Tablet of Generic Sofosbuvir and Daclatasvir: A Multicenter, Phase III Clinical Trial

Background. The combination of sofosbuvir and daclatasvir is a potent, pangenotypic regimen suitable for mass-scale hepatitis C treatment, especially in resource-limited countries where newer, expensive combinations are not available. This combination has been widely tested on genotype 4. However, Phase III trials of this combination in other genotypes have been cost prohibitive. With the introduction of generic, low-cost sofosbuvir and daclatasvir, large-scale studies in resource-limited countries are now possible. Methods. Sofosbuvir at 400 mg and daclatasvir at 60 mg were coformulated into a fixed-dose combination (FDC) tablet (Sovodak, Rojan Pharma, Tehran, Iran). Patients from 46 centers were dosed for 12 or 24 weeks with or without ribavirin, in line with existing guidelines. Responses to treatment were evaluated 12 weeks after the end of treatment (for a sustained virological response at Week 12; SVR12). Results. There were 1361 patients recruited. Overall, the patients were 21% female, with a mean age of 50 years; 39% were cirrhotic; 22% were treatment-experienced; 47% were genotype 1, 41% were genotype 3, and 2% were other genotypes. The genotype was not known in 10% of the patients. The intention-to-treat and per-protocol SVR12 rates were 94.7% and 98.8%, respectively. The safety profile was unremarkable, treatment was well tolerated, and compliance with the single-tablet regimen was excellent. Conclusions. The treatment with FDC of sofosbuvir and daclatasvir achieved high SVR12 rates, equivalent to those seen in Phase III trials of other pangenotypic options, and has been conducted at a similar scale in a representative, real-world population at a cost of under $100 per patient, which makes this combination suitable for elimination protocols in resource-limited countries. Keywords:sofosbuvir; daclatasvir; Hepatitis C; sustained virological response; generic drug

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field