Food Habits of Deer in the Black Hills

This study was conducted in two parts for Master of Science theses by the senior authors of Parts I and II. The study in the Northem Black Hills (Part I) was completed in 1968. Principal and preferred foods were determined for the winter and summer and a pasture study was conducted to measure production and utilization of foods in a typical aspen stand during the summer months. The study in the Southern Black Hills (Part II) was made in 1968 and 1969. Objectives were to determine the principal plants used by mule and white-tailed deer in fall, winter and summer. The utility of the point-analysis technique for measuring rumen contents was evaluated and the technique was applied to rumen contents examined. The studies were supported by the South Dakota Department of Game, Fish and Parks under Federal Aid Project W-75-R through the South Dakota Cooperative Wildlife Research Unit (South Dakota State University, the Bureau of Sport Fisheries and Wildlife, the South Dakota Department of Game, Fish and Parks and the Wildlife Management Institute, cooperating). Special acknowledgement is extended to the personnel of the Wildlife Habitat Project, Rocky Mountain Forest and Range Experiment Station Rapid City, for their assistance in both studies. Dr. Donald Dietz, Project Leader, and Harold E. Messner, Range Technician, were particularly helpful in the development of techniques for analysis of rumen content and the equipment used for the point-analysis method described in Part II. The assistance of William Hepworth, Director of Technical Research, Wyoming Game and Fish Department, in securing the two deer used in the pasture study described in Part I is gratefully acknowledged

Optical/Near-Infrared Observations of GRO J1744-28

We present results from a series of optical (g and r-band) and near-infrared (K'-band) observations of the region of the sky including the entire XTE and ROSAT error circles for the ``Bursting Pulsar'' GRO J1744-28. These data were taken with the Astrophysical Research Consortium's 3.5-m telescope at Apache Point Observatory and with the 2.2-m telescope at the European Southern Observatory. We see no new object, nor any significant brightening of any known object, in these error circles, with the exception of an object detected in our 8 February 1996 image. This object has already been proposed as a near-infrared counterpart to GRO J1744-28. While it is seen in only two of our ten 8 February frames, there is no evidence that this is an instrumental artifact, suggesting the possibility of near-infrared flares from GRO J1744-28, similar to those that have been reported from the Rapid Burster. The distance to the ``Bursting Pulsar'' must be more than 2 kpc, and we suggest that it is more than 7 kpc.Comment: 21 pages, 5 JPEG plates, 2 postscript figures. This paper will appear in the May 1, 1997 edition of the Astrophysical Journa

Evaluation of large volume yeast interfering RNA lure-and-kill ovitraps for attraction and control of Aedes mosquitoes

Aedes mosquitoes (Diptera: Culicidae), principle vectors of several arboviruses, typically lay eggs in man-made water-filled containers located near human dwellings. Given the widespread emergence of insecticide resistance, stable and biofriendly alternatives for mosquito larviciding are needed. Laboratory studies have demonstrated that inactivated yeast interfering RNA tablets targeting key larval developmental genes can be used to facilitate effective larvicidal activity while also promoting selective gravid female oviposition behaviour. Here we examined the efficacy of transferring this technology toward development of lure-and-kill ovitraps targeting Aedes aegypti (L.) and Aedes albopictus (Skuse) female mosquitoes. Insectary, simulated field and semi-field experiments demonstrated that two mosquito-specific yeast interfering RNA pesticides induce high levels of mortality among larvae of both species in treated large volume containers. Small-scale field trials conducted in Trinidad, West Indies demonstrated that large volume ovitrap containers baited with inactivated yeast tablets lure significantly more gravid females than traps containing only water and were highly attractive to both A. aegypti and A. albopictus females. These studies indicate that development of biorational yeast interfering RNA-baited ovitraps may represent a new tool for control of Aedes mosquitoes, including deployment in existing lure-and-kill ovitrap technologies or traditional container larviciding programs

Imaginal Discs – A New Source of Chromosomes for Genome Mapping of the Yellow Fever Mosquito Aedes aegypti

Dengue fever is an emerging health threat to as much as half of the human population around the world. No vaccines or drug treatments are currently available. Thus, disease prevention is largely based on efforts to control its major mosquito vector Ae. aegypti. Novel vector control strategies, such as population replacement with pathogen-incompetent transgenic mosquitoes, rely on detailed knowledge of the genome organization for the mosquito. However, the current genome assembly of Ae. aegypti is highly fragmented and requires additional physical mapping onto chromosomes. The absence of readable polytene chromosomes makes genome mapping for this mosquito extremely challenging. In this study, we discovered and investigated a new source of chromosomes useful for the cytogenetic analysis in Ae. aegypti – mitotic chromosomes from imaginal discs of 4th instar larvae. Using natural banding patterns of these chromosomes, we developed a new band-based approach for physical mapping of DNA probes to the precise chromosomal positions. Further application of this approach for genome mapping will greatly enhance the utility of the existing draft genome sequence assembly for Ae. aegypti and thereby facilitate application of advanced genome technologies for investigating and developing novel genetic control strategies for dengue transmission

Phase 1 study of selinexor plus carfilzomib and dexamethasone for the treatment of relapsed/refractory multiple myeloma

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/150566/1/bjh15969.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/150566/2/bjh15969_am.pd

VectorBase: a data resource for invertebrate vector genomics

VectorBase (http://www.vectorbase.org) is an NIAID-funded Bioinformatic Resource Center focused on invertebrate vectors of human pathogens. VectorBase annotates and curates vector genomes providing a web accessible integrated resource for the research community. Currently, VectorBase contains genome information for three mosquito species: Aedes aegypti, Anopheles gambiae and Culex quinquefasciatus, a body louse Pediculus humanus and a tick species Ixodes scapularis. Since our last report VectorBase has initiated a community annotation system, a microarray and gene expression repository and controlled vocabularies for anatomy and insecticide resistance. We have continued to develop both the software infrastructure and tools for interrogating the stored data

Three Keys to Success for Principals (and Their Teachers)

This is the author's accepted manuscript, post peer-review. The publisher's official version is available at: http://dx.doi.org/10.1080/00228958.2008.10516527.What is successful leadership and how can leadership concepts be applied to schools? Hundreds of books and articles and a plethora of executive seminars describe what leadership is and propose strategies for what effective leaders do. Most of these writings and presentations, however, focus on business, with much less information available about how to lead schools. In addition, there is a diversity of opinions about what makes leaders effective. This article suggests that it is possible to extract, reframe, and apply the best of what is known about leadership to help principals be more successful. Moreover, if principals are successful, teachers also are positioned to be successful, with the ultimate impact being successful student learning

The sex locus is tightly linked to factors conferring sex-specific lethal effects in the mosquito Aedes aegypti

In many taxa, sex chromosomes are heteromorphic and largely non-recombining. Evolutionary models predict that spread of recombination suppression on the Y chromosome is fueled by the accumulation of sexually antagonistic alleles in close linkage to the sex determination region. However, empirical evidence for the existence of sexually antagonistic alleles is scarce. In the mosquito Aedes aegypti, the sex-determining chromosomes are homomorphic. The region of suppressed recombination, which surrounds the male-specific sex-determining gene, remains very small, despite ancient origin of the sex chromosomes in the Aedes lineage. We conducted a genetic analysis of the A. aegypti chromosome region tightly linked to the sex locus. We used a strain with an enhanced green fluorescent protein (EGFP)-tagged transgene inserted near the male-determining gene to monitor crossing-over events close to the boundary of the sex-determining region (SDR), and to trace the inheritance pattern of the transgene in relation to sex. In a series of crossing experiments involving individuals with a recombinant sex chromosome we found developmental abnormalities leading to 1:2 sex biases, caused by lethality of half of the male or female progeny. Our results suggest that various factors causing sex-specific lethal effects are clustered within the neighborhood of the SDR, which in the affected sex are likely lost or gained through recombination, leading to death. These may include genes that are recessive lethal, vital for development and/or sexually antagonistic. The sex chromosome fragment in question represents a fascinating test case for the analysis of processes that shape stable boundaries of a non-recombining region