Proyecto de inversión para la implementación de una agencia de viajes de servicios turísticos comunitarios en el distrito de Incahuasi

Vive Tours, es una agencia de viajes dedicada a los servicios turísticos comunitarios en la comunidad de Incahuasi, la importancia del proyecto está en impulsar el turismo comunitario destacando sus recursos territoriales turísticos con la colaboración de los pobladores y de las entidades públicas; asimismo, hacer novedosos trabajos turísticos como agricultura y vivencial, de tal manera que contribuye a generar ingresos y desarrollo para las comunidades rurales ubicadas en el sector. El proyecto se justifica a partir de la falta de servicios turísticos comunitarios en la ciudad, y de las auspiciosas cifras esperadas para el turismo en Perú. La agencia de viaje será constituida con una inversión total de $35.598.80, el 50 Este proyecto determina la viabilidad, rentabilidad económica y financiera de la agencia de viajes de turismo comunitario; asimismo, está dirigido a turistas nacionales entre 25 a 60 años de edad que realicen turismo comunitario del nivel socioeconómico A y B que deseen vivir nuevas experiencias. Por otro lado, se determinó la viabilidad del proyecto, el cual se obtuvo mediante indicadores como el VAN, TIR, Análisis de sensibilidad y por último estados financieros. En conclusión, se demostró que el proyecto de inversión es viable económica y financieramente, porque se obtuvó un VANE de$ $49,491.32 y una VANF de$61,160.91, de tal forma que se alcanzó un TIR de 36% económico y 195.35% financiera

Silver as antibacterial toward Listeria monocytogenes

Listeria monocytogenes is a serious foodborne pathogen that can contaminate food during processing and can grow during food shelf-life. New types of safe and effective food contact materials embedding antimicrobial agents, like silver, can play an important role in the food industry. The present work aimed at evaluating the in vitro growth kinetics of different strains of L. monocytogenes in the presence of silver, both in its ionic and nano form. The antimicrobial effect was determined by assaying the number of culturable bacterial cells, which formed colonies after incubation in the presence of silver nanoparticles (AgNPs) or silver nitrate (AgNO(3)). Ionic release experiments were performed in parallel. A different reduction of bacterial viability between silver ionic and nano forms was observed, with a time delayed effect exerted by AgNPs. An association between antimicrobial activity and ions concentration was shown by both silver chemical forms, suggesting the major role of ions in the antimicrobial mode of action

Honey, bee pollen and vegetable oil unsaponifiables in wound healing

We would like to remark on the mechanisms and therapeutic properties of honey, bee pollen and unsaponifiable fractions of vegetable oils in wound healing

El ejercicio de la metacognición como dispositivo didáctico en el proceso de aprendizaje de la investigación

El presente trabajo describe un dispositivo didáctico para promover el desarrollo de la metacognición, durante el proceso de aprendizaje de la investigación, en estudiantes que cursan los últimos dos semestres de la carrera Licenciatura en Educación Física en el Instituto Superior de Educación Física (ISEF), sede Maldonado, Centro Universitario Regional Este de la Universidad de la República (UdelaR). Este dispositivo tiene su marco en la asignatura Seminario Tesina del Plan de Estudios 2004, la cual es concebida a su vez como un dispositivo pedagógico contenedor de éste. La metodología del seminario se implementa mediante la acción tutorial que promueve el desarrollo de estrategias metacognitivas. En este contexto, se explicitan la Pedagogía de la Investigación que se adopta, la tutoría universitaria como intervención formativa y el concepto de metacognición como pensamiento dirigido a supervisar la acción a través de una función reflexiva por excelencia. Finalmente, se presenta el análisis resultante del dispositivo didáctico implementado a fines del cursado del Seminario Tesina 2015

Mulinum spinosum and lamb meat: detecting its presence on live sheep

En Patagonia, la ganadería ovina es una de las principales actividades económicas, pero la calidad de la carne de cordero se ve afectada por el sabor desagradable que toma a causa de la ingesta de neneo (Mulinum spinosum (Cav.) Persoon, Apiaceae) que es una planta predominante, y muy accesible para el ganado en primavera-verano. Para detectar los animales afectados antes de su sacrificio, se ensayaron técnicas fitoquímicas en diferentes órganos de la planta y del animal buscando correspondencias. Se estudiaron 2 grupos (uno con neneo como componente de la dieta y el otro no) de 6 animales cada uno a los que se extrajeron muestras de carne, grasa, hígado, orina y sangre. Los compuestos responsables del efecto del neneo pertenecen al grupo de los terpenos/esteroles, el único presente tanto en las flores de neneo, como en la orina de los corderos. Los tejidos animales fueron inadecuados para detectar el neneo siendo la orina el medio más apropiado para ello. se propone un protocolo con la intención de generar una herramienta que permita desarrollar tecnologías de bajo costo para superar la dificultad, que queda a disposición de investigadores y productores para su puesta a prueba, con el fin de detectar un posible sabor desagradable previo al sacrificio, evitando así la consecuente pérdida económica.In Patagonia, sheep farming constitutes one of the major economic activities, but the quality of lamb meat is seriously affected by a disagreeable taste found in its tissues, derived from the ovine intake of neneo (Mulinum spinosum (Cav.) Persoon, Apiaceae), predominant in grazing lands easily accessed by sheep during the warm seasons. With the aim of detecting the affected animals before their slaughter, phytochemical testing techniques were applied on different organs of the plant and animal accordingly, in order to identify matches. We studied two groups (one had neneo in their diet while the other did not) of six animals each, of which were extracted samples of meat, fat, liver, urine and blood. The compounds responsible for the effect of neneo belong to the terpene/sterol group, the only present in the flowers of neneo as in the urine of lambs. Various animal tissues were inadequate to detect the presence of neneo in lambs, only urine has been identified as the most suitable to examine the metabolites. A protocol, to experiment with it, was proposed, with the aim of generate a useful tool for the development of low-cost technologies. This protocol is available to researchers and producers to put to the test, with the aim of detecting a disagreeable taste in the animals before their slaughter and avoiding the consequent economic loss.Fil: Seoane, Nicolás Fernando. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; ArgentinaFil: Ochoa, Juan. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; ArgentinaFil: Borrelli, Laura. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; ArgentinaFil: Severino, María Elena. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; ArgentinaFil: Taddeo, Héctor. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; ArgentinaFil: Giraudo, Celso. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; ArgentinaFil: Andrade Gamboa, Julio José. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina. Comision Nacional de Energia Atomica. Gerencia del Area de Investigaciones y Aplicaciones no Nucleares. Gerencia de Fisica (CAB); Argentin

Constitucionalismo(s) na América Latina e proteção dos afluentes: um estudo comparativo entre Equador e Brasil = Constitutionalism(s) in Latin America and protection of afluents: a comparative study between Ecuador and Brazil = Constitucionalismo(s) en América Latina y protección de afluentes: un estudio comparativo entre Ecuador y Brasil

- DOI: 10.30612/videre. v13i28.1459

Limited proteolysis of a disulfide-linked apoA-I dimer in reconstituted HDL.

The apolipoprotein A-I Milano (apoA-I M ) is a mo- lecular variant of apoA-I characterized by the Arg 173 → Cys substitution, leading to the formation of homodimers A-I M / A-I M. Upon interaction with palmitoyloleoylphosphatidyl- choline, A-I M /A-I M forms only two species of reconstituted HDL (rHDL) particles, with diameters of 7.8 and 12.5 nm. We used limited proteolysis to analyze the conformation of A-I M /A-I M in the two rHDL particles, in comparison with that of apoA-I in rHDL of similar size. ApoA-I in the small, 7.8-nm rHDL is degraded to a greater extent (50% after 6 h) than in the large rHDL ( � 10% degraded after 6 h). The pro- tease susceptibility of A-I M /A-I M in small and large rHDL is instead remarkably the same, with A-I M /A-I M being much more sensitive to proteolytic digestion (50% degraded after 10 min) than apoA-I. The identification of the proteolytic fragments by immunoblotting, N-terminal sequencing, and molecular mass determination, shows that the N-terminus of both proteins is resistant to proteolysis, with six cleavage sites located in the central and carboxy-terminal portions of the molecules. Cleavage in the middle of apoA-I occurs at dis- tinct sites in 7.8-nm (Lys 118 ) and 12.7-nm (Arg 123 ) rHDL, in- dicating a different conformation in small and large rHDL particles. The A-I M /A-I M instead adopts a unique and identi- cal conformation in small and large rHDL, with the carboxy- terminal portion of the molecule being remarkably more ac- cessible to the proteases than in apoA-I. This suggests the presence of a novel carboxy-terminal domain in A-I M /A-I M , not organized in a compact structure and not shared by wild-type apoA-I, which may account for the unique functional proper- ties of A-I M /A-I M. —Calabresi, L., G. Tedeschi, C. Treu, S. Ron- chi, D. Galbiati, S. Airoldi, C. R. Sirtori, Y. Marcel, and G. Franceschini. Limited proteolysis of a disulfide-linked apoA-I dimer in reconstituted HDL. J. Lipid Res. 2001. 42: 935-942

Clinical and prognostic implications of the genetic diagnosis of hereditary NET syndromes in asymptomatic patients

Neuroendocrine tumors (NETs) can be sporadic or they can arise in complex hereditary syndromes. Patients with hereditary NETs can be identified before the development of tumors by performing genetic screenings. The aim of the study was to evaluate the clinical and prognostic impact of a preclinical genetic screening in subjects with hereditary NET syndromes. 46 subjects referred for hereditary NET syndrome [22 MEN1, 12 MEN2, 12 Familial Paragangliomatosis (FPGL)] were enrolled and divided in 2 groups (group A, 20 subjects with clinical appearance of NET before the genetic diagnosis; group B, 26 subjects with genetic diagnosis of hereditary NET syndromes before the clinical appearance of NETs). The main outcome measures were severity of disease, prognosis, and survival. The rate of surgery for MEN1-, MEN2-, FPGL4-related tumors was 90% in group A and 35% in group B (p<0.01). Both symptoms related to tumors and symptoms related to therapies were significantly less frequent in group B than in group A (p<0.05). Tumor stage was locally advanced or metastatic in 50% of group A and in no one of group B (p<0.01). The mortality rate was 25% in group A and 0% in group B (p<0.05). An early genetic screening for hereditary NET syndromes results in an improvement in clinical presentation and morbidity. A potential impact of the genetic screening on the mortality rate of these subjects is suggested and needs to be investigated in further and more appropriate studies

Revisión de Programas hospitalarios para tratar la ansiedad quirúrgica infantil: ¿Es evidente todo lo que reluce?

There are still few hospitals that consider it important to include in their services a program that helps to decrease the level of anxiety in the patients. However, recently it is possible to find different initiatives and programs in the pediatric area for this purpose. Initiatives born under the current "humanization of hospitals" trying to achieve, with greater or lesser fortune, a closeness and better assimilation of the surgical protocols present in a hospital. Personally we believe that there is nothing more human than to exercise our office. Exercising medicine and curing a disease implies humanity in itself, even if it is applied under scientific evidence and seeking an adequate effectiveness. There is nothing more human than the fact of healing others, although in that way science is prioritized over comfort and closeness. Therefore, the authors are in favor of the new, and poorly called, humanization of health but, first ensuring, proper care. We are therefore witnessing the birth of proposals and initiatives that try to reduce the anxiety level of our smaller patients, who try to bring our medical activity closer to the daily life and try to transform the instrumentalization and modernization of medicine into something understandable to all. Something positive and desirable. However we can not forget that medicine is based on the scientific method, and that without it, these initiatives can remain in a mere superficial marketing. They may even endanger the performance and health outcomes of the health care activity itself. This does not mean in any way that the aspects that concern the psyche and the emotional part of the patient are not important. In fact they are a fundamental part of the medical activity but always from a scientific and consensual point of view. That is why it is always desirable to quantify results and evaluate aspects that aim to improve our work. In the case of childhood preoperative anxiety, initiatives and protocols have been developed that try to alleviate the causes and their consequences. The development of stories, toys, the presence of parents in anesthetic induction and other actions seem to be a current trend developed by some hospitals concerned about this issue. But what is the truth about it? How are these initiatives properly developed? And above all ... What is true in all this?RESUMEN La OMS define la salud como el estado de bienestar físico, mental y social, y no solamente la ausencia de enfermedad. Es por ello que hay que cuidar las implicaciones de la actividad quirúrgica más allá de las físicas. Algunos autores defienden que únicamente la inducción anestésica produce niveles de ansiedad clínicamente visibles en un 40-60% de los pacientes que son sometidos a una anestesia. Este hecho es más evidente en la población infantil donde los mecanismos de protección frente a situaciones estresantes no están desarrollados. Para abordar este tema hay que ofrecer al personal hospitalario herramientas que funcionen, y parece, que los programas de preparación preoperatoria y algunas técnicas de distracción funcionan, todas con sus limitaciones y sus beneficios particulares Cuando se quiere implantar un programa que ayude a los niños a afrontar el proceso quirúrgico hay que hacerlo bajo un prisma basado en la evidencia científica. El principal problema al que nos enfrentamos sobre la utilidad de estos programas e iniciativas es la gran heterogeneidad que existe entre los distintos estudios, originado por la gran cantidad de variables implicadas en el proceso psicológico del ser humano. Los autores tratan de buscar respuestas a la efectividad y al origen de los programas de preparación quirúrgica que existen actualmente mediante la búsqueda y el análisis de los estudios existentes en las bases de datos: MEDLINE, Web of Knowledge, COCHRANE, EMBASE hasta diciembre de 2016 sin año de restricción. Se incluyeron aquellos trabajos que evaluaban la ansiedad perioperatoria infantil y fueran estudios randomizados, con una puntuación 4-5 en la escala de JADAD

Immunoanalytical Approach for Detecting and Identifying Ancestral Peptide Biomarkers in Early Earth Analogue Environments

Several mass spectrometry and spectroscopic techniques have been used in the search for molecular biomarkers on Mars. A major constraint is their capability to detect and identify large and complex compounds such as peptides or other biopolymers. Multiplex immunoassays can detect these com-pounds, but antibodies must be produced for a large number of sequence-dependent molecular targets. Ancestral Sequence Re-construction (ASR) followed by protein "resurrection" in the lab can help to narrow the selection of targets. Herein, we propose an immunoanalytical method to identify ancient and universally conserved protein/peptide sequences as targets for identifying ancestral biomarkers in nature. We have developed, tested, and validated this approach by producing antibodies to eight previously described ancestral resurrected proteins (three beta-lactamases, three thioredoxins, one Elongation Factor Tu, and one RuBisCO, all of them theoretically dated as Precambrian), and used them as a proxy to search for any potential feature of them that could be present in current natural environments. By fluorescent sandwich microarray immunoassays (FSMI), we have detected positive immunoreactions with antibodies to the oldest beta-lactamase and thioredoxin proteins (ca. 4 Ga) in samples from a hydrothermal environment. Fine epitope mapping and inhibitory immunoassays allowed the identification of well-conserved epitope peptide sequences that resulted from ASR and were present in the sample. We corroborated these results by metagenomic sequencing and found several genes encoding analogue proteins with significant matches to the peptide epitopes identified with the antibodies. The results demonstrated that peptides inferred from ASR studies have true counterpart analogues in Nature, which validates and strengthens the well-known ASR/protein resurrection technique and our immunoanalytical approach for investigating ancient environments and metabolisms on Earth and elsewhere