Datasets for transcriptomics, q-proteomics and phenotype microarrays of polyphosphate metabolism mutants from Escherichia coli

Indexación: Scopus.Author acknowledges Fondecyt Grants 1120209, 1121170 and Anillo ACT-1107Here, we provide the dataset associated with our research article on the polyphosphate metabolism entitled, “Multi-level evaluation of Escherichia coli polyphosphate related mutants using global transcriptomic, proteomic and phenomic analyses”. By integrating different omics levels (transcriptome, proteome and phenome), we were able to study Escherichia coli polyphosphate mutant strains (Δppk1, Δppx, and Δppk1-ppx). We have compiled here all datasets from DNA microarrys, q-proteomic (Isotope-Coded Protein Labeling, ICPL) and phenomic (Phenotype microarray) raw data we have obtained in all polyP metabolism mutants.http://www.sciencedirect.com/science/article/pii/S2352340917300860?via%3Dihu

Caracterización de semillas blancas y negras de Salvia hispanica L. (Lamiaceae)

Caracterización de semillas blancas y negras de Salvia hispanica L. (Lamiaceae)Fil: Bueno, Mirian. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentin

Estudio experimental del secado de hojas de chía (<i>Salvia hispánica</i> L.)

Las hojas de Salvia hispánica L. chía presentan potencial uso medicinal, en perfumería y como saborizante, además de propiedades repelentes de algunos insectos, siendo escasos los estudios sobre secado y conservación de las mismas. En el presente trabajo, se analizan condiciones de secado en laboratorio y en secador solar pasivo, como asimismo se determinan características microscópicas y espectrales de tejido fresco y seco, constituyendo un avance en el conocimiento del material y metodología de estudio a efectos de su aplicación.Leaves of Salvia hispanica L. chia have potential medicinal use in perfumery and as a flavoring, in addition to some insect repellent properties, with few studies on drying and preserving them. In this paper, drying conditions in laboratory and analyze passive solar dryer, microscopic and spectral features of cool, dry tissue was also determined, providing a breakthrough in understanding the material and methodology to study effects of its application.Asociación Argentina de Energías Renovables y Medio Ambiente (ASADES

Comparative Analysis of Salmon Cell Lines and Zebrafish Primary Cell Cultures Infection with the Fish Pathogen <i>Piscirickettsia salmonis</i>

Piscirickettsia salmonis is the etiologic agent of piscirickettsiosis, a disease that causes significant losses in the salmon farming industry. In order to unveil the pathogenic mechanisms of P. salmonis, appropriate molecular and cellular studies in multiple cell lines with different origins need to be conducted. Toward that end, we established a cell viability assay that is suitable for high-throughput analysis using the alamarBlue reagent to follow the distinct stages of the bacterial infection cycle. Changes in host cell viability can be easily detected using either an absorbance- or fluorescence-based plate reader. Our method accurately tracked the infection cycle across two different Atlantic salmon-derived cell lines, with macrophage and epithelial cell properties, and zebrafish primary cell cultures. Analyses were also carried out to quantify intracellular bacterial replication in combination with fluorescence microscopy to visualize P. salmonis and cellular structures in fixed cells. In addition, dual gene expression analysis showed that the pro-inflammatory cytokines IL-6, IL-12, and TNFα were upregulated, while the cytokines IL1b and IFNγ were downregulated in the three cell culture types. The expression of the P. salmonis metal uptake and heme acquisition genes, together with the toxin and effector genes ospD3, ymt, pipB2 and pepO, were upregulated at the early and late stages of infection regardless of the cell culture type. On the other hand, Dot/Icm secretion system genes as well as stationary state and nutrient scarcity-related genes were upregulated only at the late stage of P. salmonis intracellular infection. We propose that these genes encoding putative P. salmonis virulence factors and immune-related proteins could be suitable biomarkers of P. salmonis infection. The infection protocol and cell viability assay described here provide a reliable method to compare the molecular and cellular changes induced by P. salmonis in other cell lines and has the potential to be used for high-throughput screenings of novel antimicrobials targeting this important fish intracellular pathogen