Inactivation of Brettanomyces bruxellensis by High Hydrostatic Pressure technology

Póster presentado en el XXV Congreso de la Sociedad Española de Microbiología (SEM), celebrado en Logroño del 7 al 10 de julio de 2015.Peer Reviewe

Pleiotropic functions of the tumor- and metastasis-suppressing Matrix Metalloproteinase-8 in mammary cancer in MMTV-PyMT transgenic mice

Matrix metalloproteinase-8 (MMP-8; neutrophil collagenase) is an important regulator of innate immunity which has onco-suppressive actions in numerous tumor types

Synaptic proximity enables NMDAR signalling to promote brain metastasis.

Metastasis-the disseminated growth of tumours in distant organs-underlies cancer mortality. Breast-to-brain metastasis (B2BM) is a common and disruptive form of cancer and is prevalent in the aggressive basal-like subtype, but is also found at varying frequencies in all cancer subtypes. Previous studies revealed parameters of breast cancer metastasis to the brain, but its preference for this site remains an enigma. Here we show that B2BM cells co-opt a neuronal signalling pathway that was recently implicated in invasive tumour growth, involving activation by glutamate ligands of N-methyl-D-aspartate receptors (NMDARs), which is key in model systems for metastatic colonization of the brain and is associated with poor prognosis. Whereas NMDAR activation is autocrine in some primary tumour types, human and mouse B2BM cells express receptors but secrete insufficient glutamate to induce signalling, which is instead achieved by the formation of pseudo-tripartite synapses between cancer cells and glutamatergic neurons, presenting a rationale for brain metastasis.This work was principally supported by grants from the Swiss National Science Foundation and the European Research Council, and by a gift from the Biltema Foundation that was administered by the ISREC Foundation, Lausanne, Switzerland

Preclinical Models of Brain Metastasis

Research at the Brain Metastasis Group is supported by MINECO grants MINECO-Retos SAF2017-89643-R (M.V.), Bristol-Myers Squibb- Melanoma Research Alliance Young Investigator Award 2017 (498103) (M.V.), Beug Foundation’s Prize for Metastasis Research 2017 (M.V.), Fundación Ramón Areces (CIVP19S8163) (M.V.), Worldwide Cancer Research (19-0177) (M.V.), H2020-FETOPEN (828972)N

Joint protection of a military formation using heterogeneous sensors in a mobile ad hoc network: Concept and field tests

This paper presents a concept on how a military formation can be jointly protected by linking available single-platform protection systems. To achieve this, a mobile ad hoc network is established between different vehicles carrying heterogeneous sensors, specifically acoustic and ESM sensors, emulating the protection systems. Distributed data fusion is applied to provide a situation overview for localizing a threat. To substantiate the concept, results from field tests regarding network, sensor, and data fusion functionality are presented

Coalition networks for secure information sharing (CoNSIS)

The multilateral CONSIS project is related to the migration towards Network Enabled Capabilities (NEC) in the participating countries. As such, CoNSIS aligns with the overarching objective of the NATO NEC (NNEC) to enhance the Alliance's ability to federate various capabilities at all levels, military (strategic to tactical) and civilian through networking and information infrastructure. Providing security and efficient network management have been important aspects of this work. The work has been a combination of theoretical studies and field experiments in order to get hands-on experience with the involved technologies. This possibility of addressing radios, networks, SOA, security and management together, has given us knowledge on how the different technologies affect each other and how they may be combined. A follow up project (CoNSIS phase II) is being planned with startup in 2014

High pressure processing assisted enzymatic hydrolysis - An innovative approach for the reduction of soy immunoreactivity

Soybean (Glycinemax (L.)MERR.) is recognized as a potent food allergen causing one of the most frequent food allergies worldwide. The effect of high pressure processing (HPP) prior to and during enzymatic hydrolysis using the enzyme preparation Flavourzyme® on the degree of hydrolysis (DH), molecular weight distribution (SDS-PAGE) and β-conglycinin (Glym5) immune reactivity of soy protein isolate (SPI)was studied. Enzymatic hydrolysis was carried out at atmospheric pressure (0.1MPa) and HPP (100–600MPa) at 50 °C for 15 min. Pressures higher than 300 MPa enhanced the degradation of Gly m5, which was confirmed by SDS-PAGE and LC-MS/MS analyses. The immunoreactivity of the samples was assessed by invitro sandwich ELISA using mouse monoclonal anti-Gly m5 antibodies. Depending on the antibody tested, the residual immunoreactivity was completely inhibited or significantly impaired up to 99.5% applying HPP during hydrolysis at 400 and 500 MPa. By means of principal component analysis, the beany and green off-flavors characteristic for unprocessed SPI could be reduced by pressure enhanced hydrolysis at 400–500 MPa. The resulting hydrolysates possessed improved protein solubility, foaming activities and oil-binding capacities, which were improved by 45%, 66%, and 210%, respectively. HPP prior to and during enzymatic hydrolysis at 400–500MPa constitutes an innovative approach for the production of low-allergen food ingredients that combine good taste and enhanced functional properties. Industrial relevance: Food allergy has emerged in the last years as the incidence and prevalence are rising dramatically. Up to now, enzymatic hydrolysis is the only feasible method to mitigate soy allergy. However, the major drawback associated with enzymatic hydrolysis is the incomplete destruction of allergenic epitopes and the formation of a strong bitter taste. This research activity demonstrates that high pressure assisted enzymatic hydrolysis using the enzyme preparation Flavourzyme effectively reduces the immunoreactivity of soy proteins. Degree of hydrolysis analysis, SDS-PAGE, mass spectrometry as well as sandwich ELISAwithmousemonoclonalanti-Glym5antibodieshave been applied to analyze the destruction of allergenic proteins as well as to determine the residual immunoreactivity. This study provides preliminary evidence that this innovative combination process of high pressure and enzymatic hydrolysis has great potential to produce tasty low-allergen soy-based food ingredients with good physicochemical properties, i.e. protein solubility and foam ability