Effects Of Propranolol And Paclitaxel On Angiogenesis In Breast Cancer Cell Lines

Purpose: The aim of this study was to investigate the effect of propranolol (PR), which is used in infantile hemangiomas, and paclitaxel (PX), which is widely used as an chemotherapeutic agent, on cancer cells. Materials and Methods: That the cells counted with trypan blue the doubling time were determined. Also with MTT assay were analyzed the cytotoxic effect and IC50 value of drugs. In the breast cancer cell lines which are differents with regard to invasion (MDA-MB-231 and MCF-7) anti-VEGF, anti-eNOS, anti-iNOS and anti-ERK1/2 primer antibodies investigated by using immunohistochemical methods. To evaluation of immunoreactivity was used the H-scoring system. Results: With MTT test, IC50 values are applied to the cells dosage for MDA-MB-231 cells; PX: 5 nmol, PR: 50 mu m, and for MCF-7 cells PX: 3,7 nmol, PR: 50 mu m, were established. In immunohistochemical application, immunoreactivity of control group was increased with strong and/or stronger in the cancer cells, while those of in PX, PR and combine treatment was decreased either significant or very significant. Conclusion: With this study, application of anti chemotherapeutic therapy which is paclitaxel, in additon with anti angiogenic therapy in the treatment of breast cancer, vascular vasodilation, cell proliferation, migration, survival ultimately thought to be important in the prevention or reduce of angiogenesis.Wo

Effects of the Glutamine on the Neuronal Cell Death in rat Ischemia-reperfusion Model

ATILLA, PERGIN/0000-0001-5132-0002WOS: 000347120800005Background: The aim of this study was to explore the effects of glutamine in brain ischemia/reperfusion model in rat. Methods: Right common carotid arteries of 24 Wistar albino rats were clamped for a duration of 30 minutes. Two hours later, except CONTROL group, glutamine was infused into left femoral vein of rats in GLIV group; and glutamine and normal saline was administered into cisterna magna of rats in GLIS and SFIS groups, respectively. After 7 days, all animals were decapitated and each brain was divided into two hemispheres for histopathological and biochemical evaluation. The right hemisphere was called "Hypoxia/Reperfusion side (HRS)" and the left hemisphere was called " Toxicity side (TS)". Results: In TS and HRS, degenerated neuron counts of GLIV groups were significantly higher than other groups' values. Degenerated neuron count values of TS were significantly lower than HRS values for GLIS, and SFIS groups, but the results of GLIV group in TS did not different from the GLIV group in HRS. LPO levels of TS and HRS of the groups was not statistically significant. Conclusion: This study results showed that glutamine had no beneficial effect to the hypoxia/reperfusion injury in rat model

Cytotoxic Effect Of Endodontic Irrigants In Vitro

Background Cytotoxicity of root canal irrigants is important due to their close contact with host tissues. The aim of this study was to assess the cytotoxic effect of NaOCl 3%, Chx 2%, and MTAD on rat periodontal ligament fibroblasts, at 0.1 and 100 μl/mL, using WST-1 colorimetric method. Material/Method Rat ligamental fibroblasts were exposed to the irrigants and their viability was assessed after 1, 24, 48, and 72 h. The measurements were determined using WST-1 assay, using a micro ELISA reader. Results At 100 ml/L all 3 irrigants were strongly cytotoxic, although CHX was less so than NaOCl and MTAD. At the 0.1 ml/L concentration, NaOCl and MTAD were only moderately cytotoxic, whereas Chx was highly deleterious to cell viability at all time points. There was a significant influence of the dilution rate of the substance, because the odds ratio for cell viability being over 50% was increased 51 times between the 100 ml/L and 0.1 ml/L dilutions. Conclusions It seems that irrigating solutions should be used at lower concentrations to enhance cell viability.PubMe

In Vitro Comparison Of Cytotoxicity Of Four Root Canal Sealers On Human Gingival Fibroblasts

The goal of this in vitro study was to evaluate the relative biocompatibility of four endodontic sealers on the cell culture of the human fibroblast through cytotoxicity.PubMe

Netherton's Syndrome: An Ultrastructural Study: Case Report

Hair samples taken from 12-year-old boy prediagnosed with Netherton's syndrome were evaluated by using light and electron microscopic methods. Hair samples were prepared according to routine electron microscopic procedures. Semi-thin sections were stained with methylene blue-azure II, thin sections were contrasted with uranyl acetate-lead citrate. Besides atopy symptoms, hyperparakeratosis and inflammatory cell infiltration were seen in dermis. Results were in accordance with icthyosis linearis circumflexa. In cortex portion of hair, nodulary thickening and swelling were observed. There was a punctured proximal portion like a telescope in distal hair shaft segment. Cells that make up hair cortex were seen to be loosely arranged and there was detachment between cells exterior of cuticle. In evaluating all results trichorrhexis invaginata was identified. Patients given long term therapy for atopic dermatitis, which have breaking of hair in addition to icthyosis, Netherton's syndrome should be thought and investigation of hair samples for supporting the diagnosis is important

Effects Of Intracisternal Dexmedetomidine On Cerebral Neuronal Cells In Rat: A Preliminary Study

AIM:The aim was to investigate whether dexmedetomidine had a toxic effect on cerebral neurons when it was administered centrally into the cerebrospinal fluid by the intracisternal route. MATERIAL and METHODS: Eighteen rats were anesthetized and the right femoral artery was cannulated. Mean arterial pressures, heart rates, arterial carbon dioxide tension, arterial oxygen tension, and blood pH were recorded. When the free cerebrospinal fluid flow was seen, 0.1 ml normal saline (Group SIC, n=6) or 9 mu g/kg diluted dexmedetomidine in 0.1 ml volume (Group DIC, n=6) was administered into the cisterna magna of rats. After 24 hours, the whole body blood was collected for measurement of plasma lipid peroxidation (LPO) levels. The hippocampal formations used for histopathological examination and measurement of tissue LPO levels. RESULTS: There was a statistically significant difference between the DIC/SIC groups and DIC/CONTROL groups regarding the brain LPO levels (p=0.002, p<0.001, respectively). Plasma LPO levels were statistically different between the CONTROL/DIC groups, CONTROL/SIC groups, DIC/ SIC groups (p=0.002, p=0.047, p=0.025, respectively),The picnotic neuron counts were different between the CONTROL/SIC groups, CONTROL/ DIC groups, DIC/SIC groups (p<0.001, p=0.001, p=0.024, respectively). CONCLUSION: In conclusion, dexmedetomidine had a toxic effect on cerebral neurons when it was administered centrally into the cerebrospinal fluid by the intracisternal route.WoSScopu

Effects Of Intracisternal And Intravenous Dexmedetomidine On Ischemia-Induced Brain Injury In Rat: A Comparative Study

AIM: To compare the effect of dexmedetomidine administered by intracisternal route with by intravenous route on brain tissue of rat after incomplete cerebral ischemia. MATERIAL and METHODS: Cerebral ischemia was produced by the combination of right common carotid artery occlusion and hemorrhagic hypotension during 30 minutes. Thirty minutes before the ischemia, 0.1 ml 0.9% NaCl (Group SIC, n=6) or 9 mu g/kg dexmedetomidine (Group DIC, n=6) was administered into the cisterna magna. For the intravenous groups, 9 mu g/kg dexmedetomidine (Group DIV, n=6) or 0.9% NaCl (Group CONTROL, n=6) 5 ml/kg/h was given in 2 hours. After 24 hours, the lipid peroxidation levels were measured in the brain tissue and plasma. Hippocampal formations were used for histopathological examination. RESULTS: Intravenous dexmedetomidine produced a decrease in baseline mean arterial blood pressure and plasma glucose concentrations. There was a significant difference between the DIV group and DIC, SIC, CONTROL groups regarding the brain lipid peroxidation levels (p<0.001, p<0.001, p=0.001, respectively), and regarding the picnotic neuronal cell count (p<0.001, p=0.01, p=0.009, respectively). Mean plasma lipid peroxidation levels of the DIV group was different from the DIC group (p=0.003). CONCLUSION: Systemically administered dexmedetomidine had neuroprotective effect in ischemia-induced neuronal damage, but centrally administered dexmedetomidine did not.WoSScopu

Comparison of the Effects of Contractubex (R) Gel in an Experimental Model of Scar Formation in Rats: An Immunohistochemical and Ultrastructural Study

Background: Contractubex gel, a commercial treatment for scars, consists of a mixture of onion extract (cepea extract), heparin sodium, and allantoin. It exerts a softening and smoothing effect on indurated, hypertrophic, painful, and cosmetically-disfiguring scar tissue

Using Erbium-Doped Yttrium Aluminum Garnet Laser Irradiation In Different Energy Output Levels Versus Ultrasonic In Removal Of Root Canal Filling Materials In Endodontic Retreatment

Objectives: The objective of this study was to evaluate the efficacy of erbium-doped yttrium aluminum garnet (Er:YAG) laser irradiation in different energy outputs versus ultrasonic in gutta-percha removal during the endodontic retreatment. Materials and Methods: A total of 21 extracted human lower premolars were divided into three groups (n = 7). Following the standardized preparation of the root canals with Wave One Rotary system and obturation with gutta-percha: Group I was treated with ultrasonic, Group II by Er:YAG laser with 40 mJ/Pulse, and Group III by Er:YAG laser with 50 mJ/Pulse for the removal of gutta-percha from the canals. Two extra teeth were treated by Er:YAG laser with 135 mJ/Pulse as control group. For all groups, time for gutta-percha removal was recorded. Samples were then splited into two halves and tested by scanning electron microscope and stereomicroscopic evaluation under different magnification power to observe the efficacy of each method used in the removal of gutta-percha. Results: Statistical analysis of Kruskal–Wallis suggested that there are significant difference between the groups in relation to removal time (P 0.05), but ultrasonic versus 40 and/or 50 mJ laser outputs were significantly different (P < 0.05). Conclusions: Er:YAG laser beam was not so efficient when compared to ultrasonic to reach the deeper parts of the canals as it was asserted, thermal side effects and burning damages were observed on the root canal dentinal walls. Moreover, the delivery system was not flexible enough to compensate the curvature of the canal system even though we used more straight canals as the sample ones as well as more time-consuming than the ultrasonic and more clinical time, rendering it to be less efficient in the removal of the obturation material during endodontic retreatment procedures.PubMedScopu