P10-10. HIV-1 Gag virus like particles pseudotyped with CD40 ligand to stimulate innate immune responses

ISSN:1742-469

Global optimization of silicon nanoclusters

A new method is presented for the computation of the lowest energy configurations of atomic clusters. It is based on recently developed set oriented numerical algorithms for the global optimization of nonlinear functions. Its underlying idea is to combine multilevel subdivision techniques for the computation of fixed points of dynamical systems with well-known branch and bound methods. We describe how this method can be used to find global minima of silicon nanoclusters in the self-consistent charge tight-binding-density-functional (SCC-DFTB) energy surface. Due to the insufficient experimental evidence of structures of silicon clusters, local minima that are near to the global minimum, are also important

Global optimization of silicon nanoclusters 1 PAPER REFERENCE: 0358

A new method is presented for the computation of the lowest energy configurations of atomic clusters. It is based on recently developed set oriented numerical algorithms for the global optimization of nonlinear functions. Its underlying idea is to combine multilevel subdivision techniques for the computation of fixed points of dynamical systems with well known branch and bound methods. We describe how this method can be used to find global minima of silicon nanoclusters in the SCC-DFTB energy surface. Due to the insufficient experimental evidence of structures of silicon clusters, local minima that are near to the global minimum, are also important

Basal cells as stem cells of the mouse trachea and human airway epithelium

The pseudostratified epithelium of the mouse trachea and human airways contains a population of basal cells expressing Trp-63 (p63) and cytokeratins 5 (Krt5) and Krt14. Using a KRT5-CreERT2 transgenic mouse line for lineage tracing, we show that basal cells generate differentiated cells during postnatal growth and in the adult during both steady state and epithelial repair. We have fractionated mouse basal cells by FACS and identified 627 genes preferentially expressed in a basal subpopulation vs. non-BCs. Analysis reveals potential mechanisms regulating basal cells and allows comparison with other epithelial stem cells. To study basal cell behaviors, we describe a simple in vitro clonal sphere-forming assay in which mouse basal cells self-renew and generate luminal cells, including differentiated ciliated cells, in the absence of stroma. The transcriptional profile identified 2 cell-surface markers, ITGA6 and NGFR, which can be used in combination to purify human lung basal cells by FACS. Like those from the mouse trachea, human airway basal cells both self-renew and generate luminal daughters in the sphere-forming assay