Bredl, S

Köstler, J

Sertl, S

Wagner, R

Wiesel, M

Wild, J

Retrovirology

English

PubMed

Köstler, Josef

Bredl, Simon

Sertl, Simone

Wiesel, Melanie

Wild, Jens

Wagner, Ralf

Repository for Publications and Research Data

HIV-1 Gag virus like particles pseudotyped with CD40 ligand to stimulate innate immune responses

J Köstler

S Bredl

S Sertl

M Wiesel

J Wild

R Wagner

Springer - Publisher Connector

P10-10. HIV-1 Gag virus like particles pseudotyped with CD40 ligand to stimulate innate immune responses

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BioMed Central
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Retrovirology
Open AccessPoster presentation
P10-10. HIV-1 Gag virus like particles pseudotyped with CD40 
ligand to stimulate innate immune responses
J Köstler2, S Bredl2, S Sertl2, M Wiesel1, J Wild2 and R Wagner*2
Address: 1Institute of Microbiology, ETH, Zürich, Switzerland and 2Molecular Microbiology and Gene Therapy, Institute of Medical Microbiology 
and Hygiene, Regensburg, Germany
* Corresponding author    
Background
Previous studies with HIV-1 Pr55Gag virus-like particles
(VLP) produced via the baculovirus expression system
showed that these VLP are able to induce strong humoral
and cellular immune responses in mice as well as non
human primates. Based on these VLP we generated mam-
malian derived VLPs and compared them regarding their
ability to maturate and activate human monocyte derived
dendritic cells (MDDC). Furthermore, we investigated the
capacity of CD40 pseudotyped mammalian VLPs to
induce the proliferation of B cells.
Methods
Pr55Gag VLP were produced (i) either in insect cells using
the baculovirus (BV) expression system or (ii) in mamma-
lian 293T cells after transient DNA transfection. MDDC
were generated and treated with the different VLPs and
were further analysed regarding maturation and activa-
tion via surface staining of CD80, CD83, CD86, HLA-DR
as well as cytokine production (IFN, TNF, IL-6, IL-10, IL-
12). Mammalian VLP were further pseudotyped with
CD40 Ligand and examined regarding their B-cell prolif-
eration capacity.
Results
Ex vivo studies on MDDCs clearly demonstrated that VLP
of BV origin, but not VLP produced in mammalian cells
triggered MDDC maturation, activation and cytokine
secretion. Furthermore CD40 ligand pseudotyped mam-
malian cell derived VLP are able to induce B-cell prolifer-
ation whereas the other VLP preparations showed no
effect.
Conclusion
These results clearly indicate that the potency of VLP to
induce innate immune responses is not an intrinsic prop-
erty of VLP. In addition pseudotyped VLPs, are the next
forefront in vaccine development to stimulate and induce
the innate immunity.
Acknowledgment
This work was supported by NIH HIVRAD
#5P01AI066287-03.
from AIDS Vaccine 2009
Paris, France. 19–22 October 2009
Published: 22 October 2009
Retrovirology 2009, 6(Suppl 3):P141 doi:10.1186/1742-4690-6-S3-P141
<supplement> <title> <p>AIDS Vaccine 2009</p> </title> <editor>Anna Laura Ross</editor> <note>Meeting abstracts – A single PDF containing all abstracts in this Supplement is available <a href="http://www.biomedcentral.com/content/files/pdf/1742-4690-6-S3-full.pdf">here</a>.</note> <url>http://www.biomedcentral.com/content/pdf/1471-2105-10-S12-info.pdf</url> </supplement>
This abstract is available from: http://www.retrovirology.com/content/6/S3/P141
© 2009 Köstler et al; licensee BioMed Central Ltd. 


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P10-10. HIV-1 Gag virus like particles pseudotyped with CD40 ligand to stimulate innate immune responses

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