Propiedades antioxidantes de algunas plantas que crecen salvajes en Turquía

In this study, the antioxidant activity of 50% aqueous methanol extracts of 38 plants growing in the Afyonkarahisar province of Turkey were evaluated by various antioxidant assay, including free radical scavenging, hydrogen peroxide (H2O2) scavenging and metal (Fe2+) chelating activities. The methanolic fruit extracts of the Cornus and Morus species (H2O2 and DPPH scavenging activities, Fe2+ chelating activity) and the methanolic leaf extracts of the Mentha species (DPPH scavenging activities) examined in the assay showed the strongest activities. These antioxidant properties depended on the concentration of samples.En este estudio, la actividad antioxidante de extractos metanólicos al 50% en agua de 38 plantas que crecen en la provincia turca de Afyonkarahisar fueron evaluados con algunos ensayos antioxidantes, incluyendo la actividad capta-dora de radicales libres y de peróxido de oxígeno (H2O2) y la actividad quelatante de metales (Fe2+). Los extractos metanólicos de frutas de las especies Cornus y Morus (actividades captadoras de H2O2 y DPPH y actividad quelatante de Fe2+) y los extractos metanólicos de hojas de especies de Mentha (actividad captadora de DPPH) son los que mostraron una actividad mayor. Estas propiedades antioxidantes dependieron de la concentración de la muestra

Plasma malondialdehyde and erythrocyte glutathione levels in workers with cement dust-exposure silicosis

Background: Chronic exposure to cement dust has been reported to lead to several health problems. Acute and chronic exposure to quartz are associated with the provocation of an inflammatory response and triggers an extensive host defense mechanism. These inflammatory reactions result in the secretions of cytokines, eicosanoids. lytic enzymes, chemotactic factors and reactive oxygen species (ROS). This Study was designed to investigate the plasma oxidant and antioxidant status in cement plant workers

The Impact of Iron Overload in Acute Leukemia: Chronic Inflammation, but Not the Presence of Nontransferrin Bound Iron is a Determinant of Oxidative Stress

In the literature, studies on the oxidant effects of nontransferrin bound iron [NTBI (eLPI assay)] during chemotherapy of acute lymphoblastic leukemia and acute myeloblastic leukemia are lacking. We established NTBI and oxidative stress determinants (OSD), iron parameters, high-sensitive C-reactive protein (hs-CRP) levels, liver tests, cumulative chemotherapeutic doses, and transfused blood in 36 children with acute leukemia throughout chemotherapy. These parameters were determined at the beginning and end of chemotherapy blocks (11 time points) and in 20 healthy children using enzyme-linked immunosorbent assay, and colorimetric and fluorometric enzymatic methods. In acute lymphoblastic leukemia, NTBI, OSD, and hs-CRP were higher than controls at 4/11, 7/11, and 9/11 time points (P0.05). OSD did not correlate with NTBI, but correlated with hs-CRP. In conclusion, NTBI is a poor predictor of OSD in acute leukemia possibly because of the heterogeneity of NTBI and chronic inflammation. Further studies are needed to delineate the pathophysiology of these diseases

Within-subject and between-subject biological variation estimates of 21 hematological parameters in 30 healthy subjects

Background: The complete blood count (CBC) is used to evaluate health status in the contexts of various clinical situations such as anemia, infection, inflammation, trauma, malignancies, etc. To ensure safe clinical application of the CBC, reliable biological variation (BV) data are required. The study aim was to define the BVs of CBC parameters employing a strict protocol

The EuBIVAS project:within- and between-subject biological variation data for serum creatinine using enzymatic and alkaline picrate methods and implications for monitoring

Abstract BACKGROUND The European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) European Biological Variation Study (EuBIVAS) has been established to deliver rigorously determined biological variation (BV) indices. EuBIVAS determined BV for serum creatinine using the enzymatic and alkaline picrate measurement methods. METHOD In total, 91 healthy individuals (38 males, 53 females; age range, 21–69 years) were bled for 10 consecutive weeks at 6 European laboratories. An equivalent protocol was followed at each center. Sera were stored at −80 °C before analysis. Analyses for each patient were performed in duplicate within a single run on an ADVIA 2400 system (San Raffaele Hospital, Milan). The data were subjected to outlier and homogeneity analysis before performing CV-ANOVA to determine BV and analytical variation (CVA) estimates with confidence intervals (CI). RESULTS The within-subject BV estimates [CVI (95% CI)] were similar for enzymatic [4.4% (4.2–4.7)] and alkaline picrate [4.7% (4.4–4.9)] methods and lower than the estimate presently available online (CVI = 5.9%). No significant male/female BV differences were found. Significant differences were observed in mean creatinine values between men and women and between Turkish individuals and those of other nationalities. Between-subject BV (CVG) estimates, stratified accordingly, produced CVG values similar to historical BV data. CVA was 1.1% for the enzymatic and 4.4% for alkaline picrate methods, indicating that alkaline picrate methods fail to fulfill analytical performance specifications for imprecision (CVAPS). CONCLUSIONS The serum creatinine CVI obtained by EuBIVAS specifies a more stringent CVAPS than previously identified. The alkaline picrate method failed to meet this CVAPS, raising questions regarding its future use. </jats:sec