Observed modes of sea surface temperature variability in the South Pacific region

The South Pacific (SP) region exerts large control on the climate of the Southern Hemisphere at many times scales. This paper identifies the main modes of interannual sea surface temperature (SST) variability in the SP which consist of a tropical-driven mode related to a horseshoe structure of positive/negative SST anomalies within midlatitudes and highly correlated to ENSO and Interdecadal Pacific Oscillation (IPO) variability, and another mode mostly confined to extratropical latitudes which is characterized by zonal propagation of SST anomalies within the South Pacific Gyre. Both modes are associated with temperature and rainfall anomalies over the continental regions of the Southern Hemisphere. Besides the leading mode which is related to well known warmer/cooler and drier/moister conditions due to its relationship with ENSO and the IPO, an inspection of the extratropical mode indicates that it is associated with distinct patterns of sea level pressure and surface temperature advection. These relationships are used here as plausible and partial explanations to the observed warming trend observed within the Southern Hemisphere during the last decades.The authors would like to thank Scott Power for his comments on an earlier version of the manuscript and the two anonymous reviewers whose suggestions led to a substantial improvement of the paper. This study was supported by Grants UBACyT-20020100100803, UBACyT-20020120300051, PIP-11220120100586 and the SPECS (GA 308378) EU-funded Project. JG-S was partially supported by the H2020-funded MSCA-IF-EF DPETNA project (GA No. 655339). The authors acknowledge the Red Española de Supercomputación (RES) and PRACE for awarding access to MareNostrum 3 at the Barcelona Supercomputing Center through the HiResClim project. The support of Virginie Guémas and Oriol Mula-Valls at the Barcelona Supercomputing Center is warmly appreciated.Peer ReviewedPostprint (author's final draft

SUMOylation regulates LKB1 localization and its oncogenic activity in liver cancer

BACKGROUND: Even though liver kinase B1 (LKB1) is usually described as a tumor suppressor in a wide variety of tissues, it has been shown that LKB1 aberrant expression is associated with bad prognosis in Hepatocellular Carcinoma (HCC). METHODS: Herein we have overexpressed LKB1 in human hepatoma cells and by using histidine pull-down assay we have investigated the role of the hypoxia-related post-translational modification of Small Ubiquitin-related Modifier (SUMO)ylation in the regulation of LKB1 oncogenic role. Molecular modelling between LKB1 and its interactors, involved in regulation of LKB1 nucleocytoplasmic shuttling and LKB1 activity, was performed. Finally, high affinity SUMO binding entities-based technology were used to validate our findings in a pre-clinical mouse model and in clinical HCC. FINDINGS: We found that in human hepatoma cells under hypoxic stress, LKB1 overexpression increases cell viability and aggressiveness in association with changes in LKB1 cellular localization. Moreover, by using site-directed mutagenesis, we have shown that LKB1 is SUMOylated by SUMO-2 at Lys178 hampering LKB1 nucleocytoplasmic shuttling and fueling hepatoma cell growth. Molecular modelling of SUMO modified LKB1 further confirmed steric impedance between SUMOylated LKB1 and the STe20-Related ADaptor cofactor (STRADα), involved in LKB1 export from the nucleus. Finally, we provide evidence that endogenous LKB1 is modified by SUMO in pre-clinical mouse models of HCC and clinical HCC, where LKB1 SUMOylation is higher in fast growing tumors. INTERPRETATION: Overall, SUMO-2 modification of LKB1 at Lys178 mediates LKB1 cellular localization and its oncogenic role in liver cancer. FUND: This work was supported by grants from NIH (US Department of Health and Human services)-R01AR001576-11A1 (J.M.M and M.L.M-C.), Gobierno Vasco-Departamento de Salud 2013111114 (to M.L.M.-C), ELKARTEK 2016, Departamento de Industria del Gobierno Vasco (to M.L.M.-C), MINECO: SAF2017-87301-R and SAF2014-52097-R integrado en el Plan Estatal de Investigación Cientifica y Técnica y Innovación 2013-2016 cofinanciado con Fondos FEDER (to M.L.M.-C and J.M.M., respectively), BFU2015-71017/BMC MINECO/FEDER, EU (to A.D.Q. and I.D.M.), BIOEF (Basque Foundation for Innovation and Health Research): EITB Maratoia BIO15/CA/014; Instituto de Salud Carlos III:PIE14/00031, integrado en el Plan Estatal de Investigación Cientifica y Técnica y Innovacion 2013-2016 cofinanciado con Fondos FEDER (to M.L.M.-C and J.M.M), Asociación Española contra el Cáncer (T.C.D, P·F-T and M.L.M-C), Daniel Alagille award from EASL (to T.C.D), Fundación Científica de la Asociación Española Contra el Cancer (AECC Scientific Foundation) Rare Tumor Calls 2017 (to M.L.M and M.A), La Caixa Foundation Program (to M.L.M), Programma di Ricerca Regione-Università 2007-2009 and 2011-2012, Regione Emilia-Romagna (to E.V.), Ramón Areces Foundation and the Andalusian Government (BIO-198) (A.D.Q. and I.D.M.), ayudas para apoyar grupos de investigación del sistema Universitario Vasco IT971-16 (P.A.), MINECO:SAF2015-64352-R (P.A.), Institut National du Cancer, FRANCE, INCa grant PLBIO16-251 (M.S.R.), MINECO - BFU2016-76872-R to (E.B.). Work produced with the support of a 2017 Leonardo Grant for Researchers and Cultural Creators, BBVA Foundation (M.V-R). Finally, Ciberehd_ISCIII_MINECO is funded by the Instituto de Salud Carlos III. We thank MINECO for the Severo Ochoa Excellence Accreditation to CIC bioGUNE (SEV-2016-0644). Funding sources had no involvement in study design; in the collection, analysis, and interpretation of data; in the writing of the report; and in the decision to submit the paper for publication

QGOLF-9 : Escala para la evaluación de la calidad percibida en los clubes de golf

The assessment of perceived quality has become important in the management of service providing organizations. The aim of this paper is to develop a brief, easy-to-apply scale with appropriate psychometric properties, which allows the incorporation of users' perceptions of the management of golf clubs. We interviewed 403 users of four 9-hole course golf clubs located in Galicia (316 males and 87 females) aged between 16 and 75 years (Mean = 47.19; SD = 13.91). For data collection, an ad hoc structured questionnaire which had 25 items grouped into 4 dimensions was used. As a result of Exploratory Factor Analysis and a subsequent Confirmatory Factor Analysis, it was possible to obtain a final scale made up of only 15 items, grouped into three dimensions. This scale, as well as being brief and easy-to-apply, has a high degree of validity (as it explains 72% of the perceived performance) and internal consistency (overall a of .91), thus making it a tool of considerable applied interest.La evaluación de la calidad percibida ha cobrado una gran importancia en la gestión de las organizaciones de prestación de servicios. El objetivo de este trabajo es desarrollar una escala con propiedades psicométricas adecuadas, breve y de fácil aplicación que permita incorporar las percepciones de los usuarios a la gestión de los clubes de golf. Fueron entrevistados 403 usuarios de 4 clubes de golf con campos de 9 hoyos ubicados en Galicia (316 hombres y 87 mujeres), con edades comprendidas entre los 16 y 75 años (Media = 47.19; DE = 13.91). Para la recogida de datos se utilizó un cuestionario estructurado ad hoc que contaba con 25 elementos agrupados en 4 dimensiones. Fruto de un Análisis Factorial Exploratorio inicial y de un Análisis Factorial Confirmatorio posterior, ha sido posible obtener una escala final compuesta por tan solo 15 ítems agrupados en tres dimensiones. Dicha escala, además de ser breve y de fácil aplicación, posee un alto grado de validez de criterio (ya que explica el 72% del desempeño percibido del club) y una elevada consistencia interna (con un a global de .91), lo que la convierte en una herramienta de notable interés aplicado

Characterization of Profiles as Management Strategies Based on the Importance and Valuation That Users Give to the Elements of the Golf Courses

The main objective of this study was to characterize golfers’ profiles based on the importance and valuation that users give to the elements of golf courses, considering the segmentation as key to making strategic decisions. This was done using a priori segmentation of the traditional style against a post hoc segmentation based on the importance that users concede to variability of elements of golf courses. Moreover, the relationship between the quality attributed to the service dimensions and the golf course global performance was examined for each of the obtained segments. The results have established certain specific characteristics of the typical golf user and two large segments of golfers were identified from Cluster Analysis (Competitives and Socials), they were made up of the following subgroups: Regular-leisure and business and matures-familiar. The causal analysis of covariance structures for each segment revealed partially different interests. This study provides useful information for managers to design management strategies that address the different characteristics, interests, and priorities of their customers and potential customers in order to win them, keep them, satisfy them, and build their loyalty, ensuring the development and sustainability of the entityS

Thickness-modulated tungsten-carbon superconducting nanostructures grown by focused ion beam induced deposition for vortex pinning up to high magnetic fields

We report efficient vortex pinning in thickness-modulated tungsten–carbon-based (W–C) nanostructures grown by focused ion beam induced deposition (FIBID). By using FIBID, W–C superconducting films have been created with thickness modulation properties exhibiting periodicity from 60 to 140 nm, leading to a strong pinning potential for the vortex lattice. This produces local minima in the resistivity up to high magnetic fields (2.2 T) in a broad temperature range due to commensurability effects between the pinning potential and the vortex lattice. The results show that the combination of single-step FIBID fabrication of superconducting nanostructures with built-in artificial pinning landscapes and the small intrinsic random pinning potential of this material produces strong periodic pinning potentials, maximizing the opportunities for the investigation of fundamental aspects in vortex science under changing external stimuli (e.g., temperature, magnetic field, electrical current)

Measuring Perceived Service Quality and Its Impact on Golf Courses Performance According to Types of Facilities and User Profile

The study was aimed at: (1) Analysing the psychometric features of the QGolf scale, (2) examining the relation between the user’s perceived quality, the club service dimensions, and the golf club performance and, (3) exploring whether a better performance could vary depending on the player’s profile and/or the type of golf course. To do so, 968 users from 13 clubs in north-western Spain golf courses were interviewed. Psychometric and theoretical findings are introduced regarding their further use in field marketing. The causal analysis of covariance structure leads us to state that the human and organisational dimension of the service is key to assess perceived quality. When comparing models, the explanatory power of the Handicap ≥ 20 model was higher than the one concerning Handicap < 20. Thus, the strategy to increase user satisfaction should be quite different depending on whether users are beginners or advanced golf players. Therefore, managers should consider the users’ profiles diversity, their specific needs, and the variety of target-groups involved, on account of the golf course’s interests. This seems the best pathway to achieve sustainability and survival in the areaS

Point of care medical device communication standars (ISO11073/IEEE1073) in patient telemonitoring

Proceeding of: European Medical and Biological Engineering and IFMBE Conference (EMBEC 2005). November 20-25, 2005. Prague, Czech Republic.This paper reviews the use of ISO11073/ IEEE1073 international standard in patient telemonitoring. The purpose of this family of standards is to allow interoperability between medical instrumentation devices and medical information systems. Its application in the field of telemonitoring can encourage telemedicine services and e-care, preventing failures and problems that are making difficult its spread (use problems, high costs of reconfigurations and actualizations). An application guide for the system engineer that want to apply them is proposed, showing the steps to follow, the benefits and handicaps in the standard implementation for different telemonitoring scenarios. The study also includes the conformity levels that have to be fulfilled, the main application points of the standard.This work was supported by projects G03/117 from Fondo de Investigaciones Sanitarias (Spanish Government) and 41/2003 from Departamento de Salud (Navarra Regional Government), and a personal grant to Miguel Galarraga from Departamento de Salud (Navarra Regional Government).No publicad

Implementación integrada de una plataforma telemática basada en estándares para monitorización de pacientes

Proceeding of: VI Jornadas de Ingeniería Telemática (JITEL 2007), Málaga, Spain, 17-19, SeptiembreThis paper presents a proof-of-concept design of an integrated solution of a telematic platform for home telemonitoring. It is end-to-end standards-based, using ISO/IEEE11073 in the client environment and EN13606 to send the information to an Electronic Healthcare Record (EHR) server. This solution has been implemented to comply with the standards available versions and tested in a laboratory environment to demonstrate the feasibility of an end-to-end standards-based platform.Este trabajo ha recibido el apoyo de proyectos de la Comisión Interministerial de Ciencia y Tecnología (CICYT) y de los Fondos Europeos de Desarrollo Regional (FEDER) TSI2004-04940-C02-01, del VI Programa Marco (Pulsers II IP) IST-27142, y del Ministerio de Educación y Ciencia (beca FPU AP-2004-3568).No publicad