Blood mRNA expression profiles of autophagy, apoptosis, and hypoxia markers on blood cardioplegia and custodiol cardioplegia groups

Introduction: Blood cardioplegia (BC) and Custodiol cardioplegia (CC) have been used for a long time in open heart surgery and are highly effective solutions. The most controversial issue among these two is whether there is any difference between them regarding myocardial damage after ischemia surgery. In this study, autophagy, apoptosis, and hypoxia markers were investigated and that way we evaluated the differences between BC and CC patients

Comparação de Soluções Cardioplégicas em Cirurgia de Revascularização Miocárdica sobre Mecanismos de Autofagia e Apoptose

Resumo Fundamento A doença arterial coronariana (DAC) devido à isquemia miocárdica causa perda permanente de tecido cardíaco. Objetivos Nosso objetivo foi demonstrar o possível dano ao miocárdio em nível molecular através dos mecanismos de autofagia e apoptose em pacientes submetidos à cirurgia de revascularização miocárdica. Métodos Um grupo recebeu uma solução de cardioplegia Custodiol e o outro grupo uma solução de cardioplegia sanguínea. Duas amostras miocárdicas foram coletadas de cada paciente durante a operação, imediatamente antes da parada cardíaca e após a liberação do pinçamento aórtico. Foram avaliadas as expressões de marcadores de autofagia e apoptose. O nível de significância estatística adotado foi de 5%. Resultados A expressão do gene BECLIN foi significativa nos tecidos miocárdicos do grupo CS (p=0,0078). Os níveis de expressão dos genes CASPASE 3, 8 e 9 foram significativamente menores no grupo CC. Os níveis pós-operatórios de TnT foram significativamente diferentes entre os grupos (p=0,0072). As expressões dos genes CASPASE 8 e CASPASE 9 foram semelhantes antes e depois do pinçamento aórtico (p=0,8552, p=0,8891). No grupo CC, os níveis de expressão gênica de CASPASE 3, CASPASE 8 e CASPASE 9 não foram significativamente diferentes em amostras de tecido coletadas após pinçamento aórtico (p=0,7354, p=0,0758, p=0,4128, respectivamente). Conclusões Com nossos achados, acreditamos que as soluções CC e CS não apresentam diferença significativa em termos de proteção miocárdica durante as operações de by-pass

Heterozygous Cc2d1a mice show sex-dependent changes in the Beclin-1/p62 ratio with impaired prefrontal cortex and hippocampal autophagy

Autism Spectrum Disorders (ASD) are a group of neurodevelopmental disorders characterized by repetitive behaviors, lack of social interaction and communication. CC2D1A is identified in patients as an autism risk gene. Recently, we suggested that heterozygous Cc2d1a mice exhibit impaired autophagy in the hippocampus. We now report the analysis of autophagy markers (Lc3, Beclin and p62) in different regions hippocampus, prefrontal cortex, hypothalamus and cerebellum, with an overall decrease in autophagy and changes in Beclin-1/p62 ratio in the hippocampus. We observed sex-dependent variations in transcripts and protein expression levels. Moreover, our analyses suggest that alterations in autophagy initiated in Cc2d1a heterozygous parents are variably transmitted to offspring, even when the offspring's genotype is wild type. Aberration in the autophagy mechanism may indirectly contribute to induce synapse alteration in the ASD brain

Reproduction and survival of overwintered and F-1 generation of two egg parasitoids of sunn pest, Eurygaster integriceps Put. (Heteroptera: Scutelleridae)

WOS: 000269553700004The differences in the reproductive biology of overwintered (OW) and F-1 generation of Trissolcus semistriatus Nees and T. festivae Victorov (Hymenoptera: Scelionidae) were investigated under laboratory conditions. The experiments were conducted to evaluate oviposition period, lifetime fecundity, female progeny, sex ratio, and longevity of OW and F-1 generation with the two species as factors. The results of this study showed that the oviposition period of F-1 generation was longer than that of OW females for both of the two parasitoid species. With the exception of F-1 generation of T. festivae, the highest daily average progeny production of females was recorded on the first day. The mean lifetime fecundity of ovipositing females were calculated as 88.8 +/- 6.13 and 111.9 +/- 6.66 for OW and F-1 generation of T. semistriatus, 85.6 +/- 6.83; 104.7 +/- 4.66 for OW and F-1 generation of T. festivae, respectively. The sex ratios of progeny for both of the species were distinctly female-biased. The effects of species, factors (OW and F-1 generation), and their interactions were significant on the sex ratio of progeny. The average longevity of OW females was 16.2 +/- 1.76 days for T. semistriatus and 16.9 +/- 1.21 for T. festivae. The average longevity of F-1 generation females was 17.5 +/- 1.46 days for T. semistriatus and 28.5 +/- 1.94 for T. festivae. Thus, some several biological characteristics of both species were compared and discussed with regard to the use of OW populations for the successful implantation of biological control and mass production

Bilateral Nevoid Hyperkeratosis of the Nipple and/or Areola: A Case Report

Nevoid hyperkeratosis of the nipple and areola (NHNA) is a rare condition, in which the skin of the nipple and/or areola becomes diffusely thickened, hyperpigmented, and covered with filiform or papular warty excrescences. Although it affects both men and woman, approximately 80% of cases ocur in women. Pathogenic mechanisms have been proposed but mainly unsubstantiated. Moreover, specific treatment guidelines do not exist partly because of the unknown pathogenesis of this disorder. We report a female patient with this rare condition. [Med-Science 2015; 4(2.000): 2252-6

The incidence of demodex in the Faculty of Science Students and measurement of these Students' knowledge level about demodex.

This study was purposed to unveil prevalence of Demodex in faculty of science students and to measure the level of knowledge about Demodex. The study population consisted of Ordu University Faculty of Science students. Participants were selected by random sampling method. A questionnaire including socio-demographic questions was applied. Standard superficial skin biopsy method was employed. In the study, after student volunteers filled out and signed Patients Informed Form, standardized surface skin biopsy from the face after signed (SYDB) samples were taken and analyzed. In the study, 34 (22.7%) of the subjects were males, 116 (77.3%) were females. The mean age of subjects with the standard deviation was 20.10 ± 2.11. 69 (46%) students were found as Demodex spp. All of the students were 1^st,2^nd,3^(rd )and 4^th year Science Faculty students. The study demonstrated that Demodex spp. is a widespread health problem in the faculty of Science students, but the level of knowledge about the Demodex spp. isnt high enough. [Med-Science 2017; 6(1.000): 30-4

Linear IgA Bullous Dermatosis: A Case Report [Lineer IgA Bulloz Dermatozu: Olgu Sunumu]

Linear IgA dermatosis(LAD) is a rare autoimmune bullous skin disease with subepidermal blister formation and linear IgA deposits along the basement membrane zone. The disease is more common in young adults. Vesiculobullous rash on the skin and mucous membranes clinically monitored. This article is presented with urticarial plaques before the clinical and admitted 17 -year-old male patient with vesiculobullous rash after about 1 year. [Med-Science 2016; 5(3.000): 845-50

Primary cutaneous anaplastic large cell lymphoma initially clinically considered to be pyoderma gangrenosum

Primary CD30+ anaplastic large cell lymphoma, which is generally seen in adults, is the second most common cutaneous T-cell lymphoma after mycosis fungoides. The lesions are characterized by red-brown plaque or nodules often with ulceration. Although usually solitary, there may occasionally be multiple lesions. Here in this case report, a 44-year old male patient with multifocal primary cutaneous anaplastic large cell lymphoma is presented. Primary cutaneous anaplastic large cell lymphoma is a rare disease especially when it is multifocal and has no systemic involvement like in this case. Therefore this case is found valuable to share. [Med-Science 2017; 6(4.000): 771-773