635 research outputs found
Investigating the role of pannexin 3 in intervertebral disc health and disease
Pannexin 3 (Panx3) is a mechanosensitive, channel-forming glycoprotein implicated in the progression of post-traumatic osteoarthritis. Despite evidence for Panx3 expression in intervertebral discs (IVDs), its function here remained unknown. Using Panx3-/- mice, this study investigated the role of Panx3 in age-associated and injury-induced models of IVD degeneration. While loss of Panx3 did not significantly impact the progression of age-associated IVD degeneration, it was associated with a protective phenotype in an injury model of IVD degeneration, specifically in the annulus fibrosus (AF). Following IVD injury, WT mice showed an accumulation of enlarged cells that appeared to contribute to disrupted AF structure. In Panx3-/- IVDs, enlarged cells were rarely detected and AF structure was retained post-injury. Interestingly, uninjured IVDs adjacent to the site of injury were disrupted in Panx3-/- mice but remained healthy in WT mice. These studies suggest a role for Panx3 in mediating adaptive AF cellular responses to acute mechanical stress
The mechano-response of murine annulus fibrosus cells to cyclic tensile strain is frequency dependent
The intervertebral disk (IVD) is a composite structure essential for spine stabilization, load bearing, and movement. Biomechanical factors are important contributors to the IVD microenvironment regulating joint homeostasis; however, the cell type-specific effectors of mechanotransduction in the IVD are not fully understood. The current study aimed to determine the effects of cyclic tensile strain (CTS) on annulus fibrosus (AF) cells and identify mechano-sensitive pathways. Using a cell-type specific reporter mouse to differentiation NP and AF cells from the murine IVD, we characterized AF cells in dynamic culture exposed to CTS (6% strain) at specific frequencies (0.1 Hz, 1.0 Hz, or 2.0 Hz). We demonstrate that our culture model maintains the phenotype of primary AF cells and that the bioreactor system delivers uniform biaxial strain across the cell culture surface. We show that exposure of AF cells to CTS induces cytoskeleton reorganization resulting in stress fiber formation, with acute exposure to CTS at 2.0 Hz inducing a significant yet transient increase ERK1/2 pathway activation. Using SYBPR-based qPCR to assess the expression of extracellular matrix (ECM) genes, ECM-remodeling genes, candidate mechano-sensitive genes, inflammatory cytokines and cell surface receptors, we demonstrated that exposure of AF cells to CTS at 0.1 Hz increased Acan, Prg4, Col1a1 and Mmp3 expression. AF cells exposed to CTS at 1.0 Hz showed a significant increase in the expression of Acan, Myc, and Tnfι. Exposure of AF cells to CTS at 2.0 Hz induced a significant increase in Acan, Prg4, Cox2, Myc, Fos, and Tnfι expression. Among the cell surface receptors assessed, AF cells exposed to CTS at 2.0 Hz showed a significant increase in Itgβ1, Itgι5, and Trpv4 expression. Our findings demonstrate that the response of AF cells to CTS is frequency dependent and suggest that mechanical loading may directly contribute to matrix remodeling and the onset of local tissue inflammation in the murine IVD
Mid-infrared sources in the ELAIS Deep X-ray Survey
We present a crossâcorrelation of the European Large Area Infrared Space Observatory (ISO) survey (ELAIS) with the ELAIS Deep Xâray Survey of the N1 and N2 fields. There are seven Chandra point sources with matches in the ELAIS Final Analysis 15âÎźm catalogue, out of a total of 28 extragalactic ISO sources present in the Chandra fields. Five of these are consistent with active galactic nuclei (AGN) giving an AGN fraction of âź19 per cent in the 15âÎźm flux range 0.8â6âmJy. We have coâadded the hard Xâray fluxes of the individually undetected ISO sources and find a low significance detection consistent with star formation in the remaining population. We combine our point source crossâcorrelation fraction with the XMMâNewton observations of the Lockman Hole and Chandra observations of the Hubble Deep Field North to constrain source count models of the midâinfrared galaxy population. The low dustâenshrouded AGN fraction in ELAIS implied by the number of crossâidentifications between the ELAIS midâinfrared sample and the Chandra point sources is encouraging for the use of midâinfrared surveys to constrain the cosmic star formation history, provided there are not further large undetected populations of Comptonâthick AGN
The SCUBA 8-mJy survey - I: Sub-millimetre maps, sources and number counts
We present maps, source lists, and number counts from the largest, unbiassed,
extragalactic sub-mm survey so far undertaken with the SCUBA camera on the
JCMT. Our maps cover 260 sq. arcmin, to a noise level S(850)=2.5 mJy/beam. We
have reduced the data using both SURF, and our own pipeline which produces
zero-footprint maps and noise images. The uncorrelated noise maps produced by
the latter approach have allowed application of a maximum-likelihood method to
measure the statistical significance of each peak, leading to properly
quantified flux-density errors for all potential sources. We detect 19 sources
with S/N > 4, 38 with S/N > 3.5, and 72 with S/N > 3. To assess completeness
and the impact of source confusion we have applied our source extraction
algorithm to a series of simulated images. The result is a new estimate of the
sub-mm source counts in the flux-density range S(850)=5-15mJy, which we compare
with other estimates, and with model predictions. Our estimate of the
cumulative source count at S(850) > 8 mJy is 320 (+80,-100) per square degree.
Assuming that the majority of sources have z > 1.5, the co-moving number
density of high-z galaxies forming stars at a rate >1000 solar masses per year
is 10^-5 per Mpc^3, with only a weak dependence on the precise redshift
distribution. This number density corresponds to that of massive ellipticals
with L > 3-4 L* at low redshift and is also the same as the co-moving number
density of comparably massive, passively-evolving objects in the redshift band
1<z<2 inferred from recent surveys of extremely red objects. Thus the bright
sub-mm sources can plausibly account for the formation of all present-day
massive ellipticals. Improved z constraints, and a proper measurement of sub-mm
clustering can refine or refute this picture.Comment: Minor revisions. 27 pages, 13 figures. Higher resolution versions of
Figs 5,6,7 and 8 are available from the autho
The ELAIS Deep X-ray Survey
We present initial follow-up results of the ELAIS Deep X-ray Survey which is
being undertaken with the Chandra and XMM-Newton Observatories. 235 X-ray
sources are detected in our two 75 ks ACIS-I observations in the well-studied
ELAIS N1 and N2 areas. 90% of the X-ray sources are identified optically to
R=26 with a median magnitude of R=24. We show that objects which are unresolved
optically (i.e. quasars) follow a correlation between their optical and X-ray
fluxes, whereas galaxies do not. We also find that the quasars with fainter
optical counterparts have harder X-ray spectra, consistent with absorption at
both wavebands. Initial spectroscopic follow-up has revealed a large fraction
of high-luminosity Type 2 quasars. The prospects for studying the evolution of
the host galaxies of X-ray selected Type 2 AGN are considered.Comment: 9 pages, 5 figures, To appear in Proceedings of XXI Moriond
Conference: "Galaxy Clusters and the High Redshift Universe Observed in
X-rays", edited by D. Neumann, F.Durret, & J. Tran Thanh Va
The ELAIS deep X-ray survey - I. Chandra source catalogue and first results
We present an analysis of two deep (75 ks) Chandra observations of the European Large Area Infrared Space Observatory (ISO) Survey (ELAIS) fields N1 and N2 as the first results from the ELAIS deep X-ray survey. This survey is being conducted in well-studied regions with extensive multiwavelength coverage. Here we present the Chandra source catalogues along with an analysis of source counts, hardness ratios and optical classifications. A total of 233 X-ray point sources are detected in addition to two soft extended sources, which are found to be associated with galaxy clusters. An overdensity of sources is found in N1 with 30 per cent more sources than N2, which we attribute to large-scale structure. A similar variance is seen between other deep Chandra surveys. The source count statistics reveal an increasing fraction of hard sources at fainter fluxes. The number of galaxy-like counterparts also increases dramatically towards fainter fluxes, consistent with the emergence of a large population of obscured sources
Observations of the Hubble Deep Field with the Infrared Space Observatory. I. Data reduction, maps and sky coverage
We present deep imaging at 6.7 micron and 15 micron from the CAM instrument
on the Infrared Space Observatory (ISO), centred on the Hubble Deep Field
(HDF). These are the deepest integrations published to date at these
wavelengths in any region of sky. We discuss the observation strategy and the
data reduction. The observed source density appears to approach the CAM
confusion limit at 15 micron, and fluctuations in the 6.7 micron sky background
may be identifiable with similar spatial fluctuations in the HDF galaxy counts.
ISO appears to be detecting comparable field galaxy populations to the HDF, and
our data yields strong evidence that future IR missions (such as SIRTF, FIRST
and WIRE) as well as SCUBA and millimetre arrays will easily detect field
galaxies out to comparably high redshifts.Comment: 7 pages, LaTeX (using mn.sty), 9 figures included as GIFs. Gzipped
Postscipt version available from http://artemis.ph.ic.ac.uk/hdf/papers/ps/.
Further information on ISO-HDF project can be found at
http://artemis.ph.ic.ac.uk/hdf
Observations of the Hubble Deep Field South with the Infrared Space Observatory - II. Associations and star formation rates
We present results from a deep mid-IR survey of the Hubble Deep Field South
(HDF-S) region performed at 7 and 15um with the CAM instrument on board ISO. We
found reliable optical/near-IR associations for 32 of the 35 sources detected
in this field by Oliver et al. (2002, Paper I): eight of them were identified
as stars, one is definitely an AGN, a second seems likely to be an AGN, too,
while the remaining 22 appear to be normal spiral or starburst galaxies. Using
model spectral energy distributions (SEDs) of similar galaxies, we compare
methods for estimating the star formation rates (SFRs) in these objects,
finding that an estimator based on integrated (3-1000um) IR luminosity
reproduces the model SFRs best. Applying this estimator to model fits to the
SEDs of our 22 spiral and starburst galaxies, we find that they are forming
stars at rates of ~1-100 M_sol/yr, with a median value of ~40M_sol/yr, assuming
an Einstein - de Sitter universe with a Hubble constant of 50 km/s/Mpc, and
star formation taking place according to a Salpeter (1955) IMF across the mass
range 0.1-100M_sol. We split the redshift range 0.0<z<0.6 into two equal-volume
bins to compute raw estimates of the star formation rate density contributed by
these sources, assuming the same cosmology and IMF as above and computing
errors based on estimated uncertainties in the SFRs of individual galaxies. We
compare these results with other estimates of the SFR density made with the
same assumptions, showing them to be consistent with the results of Flores et
al. (1999) from their ISO survey of the CFRS 1415+52 field. However, the
relatively small volume of our survey means that our SFR density estimates
suffer from a large sampling variance, implying that our results, by
themselves, do not place tight constraints on the global mean SFR density.Comment: Accepted for MNRAS. 23 pages, 10 figures (Figs. 4&6 included here as
low resolution JPEGS), latex, uses mn,epsfig. Further information and full
resolution versions of Figs 4&6 available at http://astro.ic.ac.uk/hdfs (v2:
full author list added
The coincidence and angular clustering of Chandra and SCUBA sources
NRC publication: N
Near and mid-infrared colours of star-forming galaxies in ELAIS fields
We present J and K-band near-infrared photometry of a sample of mid-infrared
sources detected by the Infrared Space Observatory (ISO) as part of the
European Large Area ISO-Survey (ELAIS) and study their classification and
star-forming properties. We have used the Preliminary ELAIS Catalogue for the
6.7 micron (LW2) and 15 micron (LW3) fluxes. All of the high-reliability LW2
sources and 80 per cent of the LW3 sources are identified in the near-IR survey
reaching K = 17.5 mag. The near- to mid-IR flux ratios can effectively be used
to separate stars from galaxies in mid-IR surveys. The stars detected in our
survey region are used to derive a new accurate calibration for the ELAIS
ISOCAM data in both the LW2 and LW3 filters. We show that near to mid-IR
colour-colour diagrams can be used to further classify galaxies, as well as
study star-formation. The ISOCAM ELAIS survey is found to mostly detect
strongly star-forming late-type galaxies, possibly starburst powered galaxies,
and it also picks out obscured AGN. The ELAIS galaxies yield an average mid-IR
flux ratio LW2/LW3 = 0.67 +/- 0.27. We discuss this [6.7/15] ratio as a star
formation tracer using ISO and IRAS data of a local comparison sample. We find
that the [K/15] ratio is also agood indicator of activity level in galaxies and
conclude that the drop in the [6.7/15] ratio seen in strongly star-forming
galaxies is a result of both an increase of 15 mic emission and an apparent
depletion of 6.7 mic emission. Near-IR data together with the mid-IR give the
possibility to estimate the relative amount of interstellar matter in the
galaxies.Comment: 18 pages, 15 figures; accepted for publication in MNRA
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