Cubosomes as potential theranostic tools

Application of nanotechnology to medicine offers a versatile approach for the design and the development of new multi-functional drug delivery systems where imaging probes, drugs, and targeting agents can be combined and applied as therapeutic/diagnostic (theranostic) tools. Furthermore, this application requires the development of biocompatible and non-toxic systems with tunable properties. Here, monoolein-based nanoparticles known as cubosomes are proposed as theranostic platform in nanomedicine. These nanoparticles show high structural stability and, because of their intrinsic nanostructure, can be loaded with hydrophobic cargos. It will be shown that cubosomes can effectively be loaded with anticancer drugs, UV-visible or NIR emitting fluorophores, while simultaneously conjugated with cells-targeting ligands. Their living cells imaging skills, addressing abilities toward HeLa cells, as well as biodistribution in vivo will be also presented. Please click Additional Files below to see the full abstract

The Open Data Kit suite, Mobile Data Collection technology as an opportunity for forest mensuration practices

This paper examines the potential for using Mobile Data Collection (MDC) as an effective database supported technology to substantially improve forest mensuration practices. Open source Open Data Kit (ODK) procedures and tools were used during a survey campaign that initiated a local forest monitoring process in the Marganai forest (Sardinia). The ODK suite is practical to use and its procedures allow authoring and use of digital survey forms without users needing software development expertise. Form design enables a high degree of customization to be achieved by means of specifying a wide range of data flow control mechanisms. ODK has proved to be a valid tool for data coherence and completeness improvements. As forestry’s contribution to regional Gross Domestic Product has dramatically decreased, forest mensuration practices have been reduced. Meeting the increased need to monitor environmental assets such as forests requires these practices to be re-evaluated. If regional public institutions took an active part in the process of enhancing forest mensuration, by contributing with open database systems acting as repositories and knowledge engines, support for MDC tools like ODK would potentially be a great opportunity to disseminate the use of the system and boost its development

Rational Design of Sustainable Liquid Microcapsules for Spontaneous Fragrance Encapsulation

The high volatility, water-immiscibility, and light/oxygen-sensitivity of most aroma compounds represent a challenge to their incorporation in liquid consumer products. Current encapsulation methods entail the use of petroleum-based materials, initiators, and crosslinkers as well as mixing, heating, and purification steps. Hence, more efficient and eco-friendly approaches to encapsulation must be sought. Herein, we propose a simple method by making use of a pre-formed amphiphilic polymer and employing the Hansen Solubility Parameters approach to determine which fragrances could be encapsulated by spontaneous coacervation in water. The coacervates do not precipitate as solids but they remain suspended as colloidally stable liquid microcapsules, as demonstrated by fluorescence correlation spectroscopy. The effective encapsulation of fragrance is proven through confocal Raman spectroscopy, while the structure of the capsules is investigated by means of cryo FIB/SEM, confocal laser scanning microscopy, and small-angle X-ray scattering

A selective cellulose/hemicellulose green solvents extraction from buckwheat chaff

A two-phase extraction process was adopted to obtain cellulose and hemicellulose from buckwheat chaff by using green solvents. We are proposing a combination of propylene carbonate (PC) and ionic liquids (ILs) in a 1:5 ratio. We compared the first-generation ILs 1-butyl-2,3-dimethylimidazolium chloride (BdmimCl), and 1-butyl-3-methyl imidazolium acetate (BmimAc) with respect to the tetrabutyl ammonium acetate (TBAAc). The cellulose and hemicellulose were, firstly, extracted into the PC/IL mixture and subsequently precipitated by water addition. All precipitate materials were analysed by FTIR-ATR and TGA, while the organic phase and the supernatant after water addition were analysed by using self-diffusion NMR. The PC green co-solvent was proven to be an exceptional candidate to replace dimethyl sulfoxide. The highest amount of precipitate material after water addition was obtained with PC-BmimAc, while PC-TBAAc was showing the highest cellulose/hemicellulose selectivity. Furthermore, a preferential interaction of the supernatant cellulose residue with PC or acetate was observed by self-diffusion NMR

Out-Of-Focus Holography Tool for the Sardinia Radio Telescope

This document represents a short guide to the out-of-focus holography package for the SRT

Forest Protection Unifies, Silviculture Divides: A Sociological Analysis of Local Stakeholders' Voices after Coppicing in the Marganai Forest (Sardinia, Italy)

Today, a forest is also understood as a real social actor with multiple-scale influences, capable of significantly conditioning the social, economic, and cultural system of a whole territory. The aim of this paper is to reconstruct and interpret the population's perception of the silvicultural activities related to traditional use of forest resources of the southwestern Sardinian Marganai State Forest. The "Marganai case" has brought to the attention of the mass media the role of this forest and its silviculture. The research was carried out via semi-structured interviews with the main stakeholders in the area. The qualitative approach in the collection and analysis of the information gathered has allowed us to reconstruct the historical-cultural and social cohesion function that the forest plays in rural communities. The results highlight that the main risks concern the erosion of the cultural forest heritage due to the abandonment of the rural dimension (mainly by the new generations, but not only), with the consequent spread of deep distortions in the perception, interpretation, and necessity of forestry activities and policy

Effect of glucocorticoid treatment on computed tomography angiography detected large-vessel inflammation in giant-cell arteritis. A prospective, longitudinal study.

Computed tomography angiography (CTA) detects signs of large-vessel vasculitis (LVV) in about 67.5% of patients with giant-cell arteritis (GCA) at the time of diagnosis and early aortic dilatation in 15%. The outcome of CTA-findings of LVV upon glucocorticoid treatment has not been prospectively evaluated. The aim of our study was to prospectively assess glucocorticoid-induced changes in CTA findings of LVV in patients with GCA. Forty biopsy-proven GCA patients evaluated by CTA at diagnosis were prospectively followed and scheduled a new CTA approximately after 1 year of treatment. Vessel wall thickening, diameter, and contrast enhancement of the aorta and its tributaries were evaluated. Results were compared to those obtained at the time of diagnosis. CTA was repeated to 35 patients after a median follow-up of 13.5 months (IQ25-75% 12.4-15.8). Arterial wall thickening was still present in 17 patients (68% of the patients who initially had LVV). The number of affected segments and wall thickness at various aortic segments significantly decreased and no patients developed new lesions, new aortic dilation or increase in previous dilation. Contrast enhancement disappeared in 15 (93.75%) of 16 patients in whom this finding could be assessed. Signs of LVV improve with treatment. While contrast enhancement resolves in the majority of patients, vessel wall thickening persists in two thirds. However, the number of affected aortic segments as well as aortic wall thickness significantly decreases. Longer follow-up is necessary to determine the clinical significance of persisting wall thickening and its relationship with relapses or subsequent development of aortic dilatation or large-vessel stenoses

MISTRAL and its KIDs

The MIllimetric Sardinia radio Telescope Receiver based on Array of Lumped elements KIDs, MISTRAL, is a cryogenic W-band (77–103 GH) LEKID camera which will be integrated at the Gregorian focus of the 64 m aperture Sardinia Radio Telescope, in Italy, in Autumn 2022. This instrument, thanks to its high angular resolution (∼13arcsec) and the wide instantaneous field of view (∼4arcmin), will allow continuum surveys of the mm-wave sky with a variety of scientific targets, spanning from extragalactic astrophysics to solar system science. In this contribution, we will describe the design of the MISTRAL camera, with a particular focus on the optimisation and test of a prototype of the focal plane

High angular resolution Sunyaev Zel’dovich observations: The case of MISTRAL

The MIllimeter Sardinia radio Telescope Receiver based on Array of Lumped elements kids, MISTRAL, is a millimetric (≃ 90GHz) multipixel camera being built for the Sardinia Radio Telescope. It is going to be a facility instrument and will sample the sky with 12 arcsec angular resolution, 4 arcmin field of view, through 408 Kinetic Inductance Detectors (KIDs). The construction and the beginning of commissioning is planned to be in 2022. MISTRAL will allow the scientific community to propose a wide variety of scientific cases including protoplanetary discs study, star forming regions, galaxies radial profiles, and high angular resolution measurements of the Sunyaev Zel'dovich (SZ) effect with the investigation of the morphology of galaxy cluster and the search for the Cosmic Web