La double réception du “Chevalier doré” (Denis Janot, 1541; Denis de Harsy, 1542; Jean Bonfons, s.d.)

Au xvie siècle le roman médiéval a fait l’objet d’une attention plus étendue qu’on ne le croit généralement. Qu’il suffise de citer la version latine de Paris et Vienne adressée par l’ambassadeur de France à Venise et futur évêque de Rieux, Jean de Pins, aux enfants du chancelier Duprat, publiée à Venise en 1516 et rééditée à Paris l’année suivante par Josse Bade, qui constitue une tentative isolée, mais significative, d’acclimatation humaniste à usage pédagogique d’un roman médiéval. Nous av..

Fatores sociodemográficos e psicológicos associados ao autocuidado e à qualidade de vida em adultos mexicanos com Diabetes Mellitus tipo 2

El propósito del presente trabajo fue identificar las variables sociodemográficas y psicológicas relacionadas con el autocuidado y la calidad de vida en adultos mexicanos con diabetes mellitus tipo 2. Se utilizó un diseño transversal en una muestra de 60 personas (93 % mujeres) entre 36 y 66 años de edad (M = 54.3, DE = 4.71) adscritas al sistema de salud pública en San Luis Potosí, México. Se midieron las variables de autocuidado, autoeficacia, conocimientos en diabetes, ansiedad, depresión y calidad de vida con la aplicación de las escalas EECAC, EAG, DKQ-24, AMAS, BDI-II y SF-36. En el análisis de resultados se utilizó un análisis de regresión lineal para evaluar el impacto de las variables medidas sobre el autocuidado y la calidad de vida. En general, el modelo explicó 33.9 % de la variación del autocuidado a través de las variables depresión (β = -.27) y autoeficacia (β = .74). El 56 % de variación en calidad de vida relacionada con la salud física se explicó a partir de las variables depresión (β = -.34) y autocuidado (β = .34). En su componente de salud mental, el 43.4 % de la variación en calidad de vida se explicó a través de la ansiedad (β = -.26) y la depresión (β = -.40). Finalmente, la percepción positiva de autoeficacia y el estado de salud libre de ansiedad y depresión resultaron ser factores determinantes para el autocuidado y la calidad de vida relacionada con la saludO propósito deste trabalho foi identificar as variáveis sociodemográficas e psicológicas relacionadas com o autocuidado e a qualidade de vida em adultos mexicanos com diabetes mellitus tipo 2. Utilizou-se um desenho transversal numa amostra de 60 pessoas (93 % mulheres) entre 36 e 66 anos (M = 54.3, DP = 4.71), vinculadas ao sistema de saúde pública em San Luis Potosí, México. Foram medidas as variáveis de autocuidado, autoeficácia, conhecimento em diabetes, ansiedade, depressão e qualidade de vida com a aplicação das escalas EECAC, EAG, DKQ-24, AMAS, BDI-II e SF-36. Na análise de resultados, utilizou-se a análise de regressão linear para avaliar o impacto das variáveis medidas sobre o autocuidado e a qualidade de vida. Em geral, o modelo explicou 33.9 % da variação do autocuidado por meio das variáveis depressão (β = -.27) e autoeficácia (β = .74). 56 % de variação em qualidade de vida relacionada com a saúde física foram explicadas a partir das variáveis depressão (β = -.34) e autocuidado (β = .34). Em seu componente de saúde mental, 43.4 % da variação em qualidade de vida foram explicadas por meio da ansiedade (β = -.26) e da depressão (β = -.40). Finalmente, a percepção positiva de autoeficácia e o estado de saúde livre de ansiedade e depressão foram fatores determinantes para o autocuidado e a qualidade de vida relacionada com a saúde.The purpose of the present study was to identify sociodemographic and psychological variables related to self-care and quality of life in Mexican adults with type 2 Diabetes Mellitus. A cross-sectional design was used in a sample of 60 people (93% women) aged between 36 and 66 years (M = 54.3, SD = 4.71) attached to the public health system in San Luis Potosí, Mexico. Self-care, self-efficacy, knowledge about diabetes, anxiety, depression and quality of life were measured using the EECAC, EAG, DKQ-24, AMAS, BDI-II and SF-36 scales. For data processing, a linear regression analysis was used to evaluate the impact of the measured variables on self-care and quality of life. In general, the model explained 33.9% of the variance of self-care through the variables depression (β = -.27) and self-efficacy (β = .74). The 56% variance in quality of life related to physical health was explained by the variables depression (β = -34) and self-care (β = .34). In their mental health component, 43.4% of variance in quality of life was explained through anxiety (β = -.26) and depression (β = -.40). Finally, the positive perception of self-efficacy and health status free from anxiety and depression were determinant factors for self-care and healthrelated quality of life

Endurance training induces apoptosis in the tumor mass in the C26-bearing mouse model

Cachexia, sarcopenia and anorexia are characterised by muscle wasting. This condition is a weakening, shrinking, and loss of muscle caused by a disease or lack of use. The loss of muscle causes a decrease in strength and inability to move compromising the quality of life. Recently we demonstrated that the skeletal muscle of endurance trained Balb/c mice release IL-6 and Hsp60 (inside exosomes) in the blood stream. We studied the expression of Hsp60 in the muscles of trained and untrained C26-bearing mice, to understand if Hsp60 was over-expression may improve muscle performance and reduce cachexia. Four different interleukins have been also studied in cachectic mice, to understand which was their effect on Hsp60 expression both in the tumor mass and the trained muscle. In the present study we demonstrated that: 1) IL-6 is released by the trained muscle; 2) IL-6 is release also by the tumor mass, 3) in animals inoculated with the C26 tumor and trained after inoculation, IL-6 is synthesized mainly by the skeletal muscle and the tumor mass undergo apoptosis

Endogenous glutamine decrease is associated with pancreatic cancer progression

Abstract Pancreatic ductal adenocarcinoma (PDAC) is becoming the second leading cause of cancer-related death in the Western world. The mortality is very high, which emphasizes the need to identify biomarkers for early detection. As glutamine metabolism alteration is a feature of PDAC, its in vivo evaluation may provide a useful tool for biomarker identification. Our aim was to identify a handy method to evaluate blood glutamine consumption in mouse models of PDAC. We quantified the in vitro glutamine uptake by Mass Spectrometry (MS) in tumor cell supernatants and showed that it was higher in PDAC compared to non-PDAC tumor and pancreatic control human cells. The increased glutamine uptake was paralleled by higher activity of most glutamine pathway-related enzymes supporting nucleotide and ATP production. Free glutamine blood levels were evaluated in orthotopic and \u202

Chimeric Rat/Human HER2 Efficiently Circumvents HER2 Tolerance in Cancer Patients.

Purpose: Despite the great success of HER2 vaccine strategies in animal models, effective clinical results have not yet been obtained. We studied the feasibility of using DNA coding for chimeric rat/human HER2 as a tool to break the unresponsiveness of T cells from patients with HER2-overexpressing tumors (HER2-CP). Experimental Design: Dendritic cells (DCs) generated from patients with HER2-overexpressing breast (n = 28) and pancreatic (n = 16) cancer were transfected with DNA plasmids that express human HER2 or heterologous rat sequences in separate plasmids or as chimeric constructs encoding rat/human HER2 fusion proteins and used to activate autologous T cells. Activation was evaluated by IFN-γ ELISPOT assay, perforin expression, and ability to halt HER2+ tumor growth in vivo. Results: Specific sustained proliferation and IFN-γ production by CD4 and CD8 T cells from HER2-CP was observed after stimulation with autologous DCs transfected with chimeric rat/human HER2 plasmids. Instead, T cells from healthy donors ( n = 22) could be easily stimulated with autologous DCs transfected with any human, rat, or chimeric rat/human HER2 plasmid. Chimeric HER2-transfected DCs from HER2-CP were also able to induce a sustained T-cell response that significantly hindered the in vivo growth of HER2+ tumors. The efficacy of chimeric plasmids in overcoming tumor-induced T-cell dysfunction relies on their ability to circumvent suppressor effects exerted by regulatory T cells (Treg) and/or interleukin (IL)-10 and TGF-β1. Conclusions: These results provide the proof of concept that chimeric rat/human HER2 plasmids can be used as effective vaccines for any HER2-CP with the advantage of being not limited to specific MHC

Hsp60 expression in skeletal muscle increases after endurance training

Adult skeletal muscle is remarkably plastic. Increased contractile activity, such as endurance exercise, elicits multiple signals to activate a large set of genes, leading to phenotypic changes in skeletal muscle, including IIb-to-IIa fiber type switching, enhanced mitochondrial biogenesis, and angiogenesis, to match physiologic capability to functional demand. Heat shock protein (Hsp) 60 is a mitochondrial protein which plays a key role in the translocation of proteins from the cytoplasm to mitochondria. Apart from its mitochondrial localization, Hsp60 has been detected in the cytoplasm, in the cellular membrane and inside exosomes [1]. In the skeletal muscle the expression of Hsp60 is fibre type specific, being expressed more in type IIa and I fibers compared to IIx and IIb. Upon endurance training Hsp60 increases particularly in type I fibers (unpublished data). To investigate the role of Hsp60 in skeletal muscle plasticity and mitochondrial biogenesis, we used three experimental models: 1) Forty-eight trained young healthy male mice; 2) in vitro C2C12, where Hsp60 was over expressed by plasmids or repressed by siRNAs; 3) in vivo transfected muscles where Hsp60 was over expressed by plasmids and inhibited by siRNAs