110 research outputs found
BPA Endocrine Disruptor Detection at the Cutting Edge: FPIA and ELISA Immunoassays
BPA is a chemical commonly used in the production of polymer-based materials that can have detrimental effects on the thyroid gland and impact human reproductive health. Various expensive methods, such as liquid and gas chromatography, have been suggested for detecting BPA. The fluorescence polarization immunoassay (FPIA) is an inexpensive and efficient homogeneous mix-and-read method that allows for high-throughput screening. FPIA offers high specificity and sensitivity and can be carried out in a single phase within a timeframe of 20â30 min. In this study, new tracer molecules were designed that linked the fluorescein fluorophore with and without a spacer to the bisphenol A moiety. To assess the influence of the C6 spacer on the sensitivity of an assay based on the respective antibody, haptenâprotein conjugates were synthesized and assessed for performance in an ELISA setup, and this resulted in a highly sensitive assay with a detection limit of 0.05 g/L. The lowest limit of detection was reached by employing the spacer derivate in the FPIA and was 1.0 ÎŒg/L, working range from 2 to 155 ÎŒg/L. The validation of the methods was conducted using actual samples compared to LCâMS/MS, which served as the reference method. The FPIA and ELISA both demonstrated satisfactory concordance.BAMâs MI-1 programPeer Reviewe
Fluorescence polarization immunoassay for the determination of diclofenac in wastewater
Pharmacologically active compounds are often detected in wastewater and surface waters. The nonsteroidal anti-inflammatory drug diclofenac (DCF) was included in the European watch list of substances that requires its environmental monitoring in the member states. DCF may harmfully influence the ecosystem already at concentrations â€â1 Όg Lâ1. The fast and easy quantification of DCF is becoming a subject of global importance. Fluorescence polarization immunoassay (FPIA) is a homogeneous mix-and-read method which does not require the immobilization of reagents. FPIA can be performed in one phase within 20â30 min, making it possible to analyse wastewater without any complicated pre-treatment. In this study, new tracer molecules with different structures, linking fluorophores to derivatives of the analyte, were synthesized, three homologous tracers based on DCF, two including a C6 spacer, and one heterologous tracer derived from 5-hydroxy-DCF. The tracer molecules were thoroughly assessed for performance. Regarding sensitivity of the FPIA, the lowest limit of detection reached was 2.0 Όg Lâ1 with a working range up to 870 Όg Lâ1. The method was validated for real wastewater samples against LC-MS/MS as reference method with good agreement of both methods.
Graphical abstractBundesanstalt fĂŒr Materialforschung und -PrĂŒfung
http://dx.doi.org/10.13039/100009553Peer Reviewe
Fluorescence polarization immunoassay for rapid screening of the pesticides thiabendazole and tetraconazole in wheat
[EN] Fluorescence polarization immunoassays (FPIAs) for thiabendazole and tetraconazole were first developed. Tracers for FPIAs of thiabendazole and tetraconazole were synthesized and the tracers' structures were confirmed by HPLC-MS/MS. The 4-aminomethylfluorescein-labeled tracers allowed achieving the best assay sensitivity and minimum reagent consumption in comparison with aminofluorescein-labeled and alkyldiaminefluoresceinthiocarbamyl-labeled tracers. Measurements of fluorescence polarization were performed using a portable device. The developed FPIA methods were applied for the analysis of wheat. Fast and simple sample preparation technique earlier developed by authors for pesticides was adapted for thiabendazole and tetraconazole. The limits of detection of thiabendazole and tetraconazole in wheat were 20 and 200g/kg, and the lower limits of quantification were 40 and 600g/kg, respectively. The recovery test was performed by two methodsFPIA and HPLC-MS/MS. The results obtained by FPIA correlated well with those obtained by HPLC-MS/MS (r(2)=0.9985 for thiabendazole, r(2)=0.9952 for tetraconazole). Average recoveries of thiabendazole and tetraconazole were 744% and 723% by FPIA, and average recoveries of thiabendazole and tetraconazole were 86 +/- 2% and 74 +/- 1% by HPLC-MS/MS (n=15).The work was financially supported by the Russian Science Foundation (project No. 14-16-00149).Boroduleva, AY.; Manclus Ciscar, JJ.; Montoya, Ă.; Eremin, SA. (2018). Fluorescence polarization immunoassay for rapid screening of the pesticides thiabendazole and tetraconazole in wheat. Analytical and Bioanalytical Chemistry. 410(26):6923-6934. https://doi.org/10.1007/s00216-018-1296-zS6923693441026Robinson HJ, Stoerk HC, Graessle OE. Studies on the toxicologic and pharmacologic properties of thiabendazole. Toxicol Appl Pharmacol. 1965;7:53â63.Abbassy MA, Marzouk MA, Nasr HM, Mansy AS. Effect of imidacloprid and tetraconazole on various hematological and biochemical parameters in male albino rats (Rattus norvegious). J Pol Sci Pub Aff. 2014;2:7.European Commission, Regulation (EC) No 2017/1164 amending Annexes II and III to Regulation (EC) No 396/2005 of the European Parliament and of the Council as regards maximum residue levels for acrinathrin, metalaxyl and thiabendazole in or on certain products. Off J EU L170. 2017:3â30.Hygienic standard GN 1.2.3539-18. Hygienic standards for pesticide residues in environmental samples (list). 2018. In Russian. â© http://docs.cntd.ru/document/557532326 . Accessed 07.07.2018).European Commission, Regulation (EC) No 822/2009 amending Annexes II, III and IV to Regulation (EC) No 396/2005 of the European Parliament and of the Council as regards maximum residue levels for azoxystrobin, atrazine, chlormequat, cyprodinil, dithiocarbamates, fludioxonil, fluroxypyr, indoxacarb, mandipropamid, potassium tri-iodide, spirotetramat, tetraconazole, and thiram in or on certain products. Off J EU L239. 2009:5â45.GarcĂa-FernĂĄndez M, DĂaz-Ălvarez M, MartĂn-Esteban A. Molecularly imprinted magnetic nanoparticles for the micro solid-phase extraction of thiabendazole from citrus samples. J Sep Sci. 2017;40:2638â44.Yu QW, Sun H, Wang K, He HB, Feng YQ. Monitoring of carbendazim and thiabendazole in fruits and vegetables by SiO2@ NiO-based solid-phase extraction coupled to high-performance liquid chromatography-fluorescence detector. Food Anal Methods. 2017;10:2892â901.Alves AA, Rodrigues AS, Barros EBP, Uekane TM, Bizzo HR, Rezende CM. Determination of pesticides residues in Brazilian grape juices using GC-MS-SIM. Food Anal Methods. 2014;7:1834â9.Zhang H, Qian M, Wang X, Wang X, Xu H, Qi P, et al. Analysis of tebuconazole and tetraconazole enantiomers by chiral HPLC-MS/MS and application to measure enantioselective degradation in strawberries. Food Anal Methods. 2012;5:1342â8.Bordagaray A, GarcĂa-Arrona R, MillĂĄn E. Development and application of a screening method for triazole fungicide determination in liquid and fruit samples using solid-phase microextraction and HPLC-DAD. Anal Methods. 2013;5:2565â71.Aquino A, Navickiene S. MSPD procedure for determination of carbofuran, pyrimethanil and tetraconazole residues in banana by GCâMS. Chromatographia. 2009;70:1265â9.Dankwardt A, Pullen S, Hock B. Immunoassays: applications for the aquatic environment. In: Wells PG, Lee K, Blaise C, editors. Microscale testing in aquatic toxicology. Boca Raton: CRC Press; 2018. p. 13â29.Wells MJM, Bell KY, Traexler KA, Pellegrin M-L, Morse A. Emerging pollutants. Water Environ Res. 2011;82(10):2095â70.Abad A, ManclĂșs JJ, Moreno MJ, Montoya A. Determination of thiabendazole in fruit juices by a new monoclonal enzyme immunoassay. J AOAC Int. 2001;84:156â61.΀sialla Z, Ucles-Moreno A, Petrou P, Fernandez-Alba AR, Îakabakos SE. Development of an indirect enzyme immunoassay for the determination of thiabendazole in white and red wines. Int J Environ Anal Chem. 2015;95:1299â309.UclĂ©s A, GarcĂa AV, GarcĂa MDG, del Real AMA, FernĂĄndez-Alba AR. Benzimidazole and imidazole fungicide analysis in grape and wine samples using a competitive enzyme-linked immunosorbent assay. Anal Methods. 2015;7:9158â65.BlaĆŸkovĂĄ M, Rauch P, Fukal L. Strip-based immunoassay for rapid detection of thiabendazole. Biosens Bioelectron. 2010;25:2122â8.Estevez MC, Belenguer J, Gomez-Montes S, Miralles J, Escuela AM, Montoya A, et al. Indirect competitive immunoassay for the detection of fungicide thiabendazole in whole orange samples by surface plasmon resonance. Analyst. 2012;137:5659â65.Cairoli S, Arnoldi A, Pagani S. Enzyme-linked immunosorbent assay for the quantitation of the fungicide tetraconazole in fruits and fruit juices. J Agric Food Chem. 1996;44:3849â54.ManclĂșs JJ, Moreno MJ, Plana E, Montoya A. Development of monoclonal immunoassays for the determination of triazole fungicides in fruit juices. J Agric Food Chem. 2008;56:8793â800.Plana E, Moreno MJ, Montoya Ă, ManclĂșs JJ. Development and application of recombinant antibody-based immunoassays to tetraconazole residue analysis in fruit juices. Food Chem. 2014;143:205â13.Feng J, Hu Y, Grant E, Lu X. Determination of thiabendazole in orange juice using an MISPE-SERS chemosensor. Food Chem. 2018;239:816â22.Smith DS, Eremin SA. Fluorescence polarization immunoassays and related methods for simple, high-throughput screening of small molecules. Anal Bioanal Chem. 2008;391:1499â07.Eremin SA, Smith DS. Fluorescence polarization immunoassays for pesticides. Comb Chem High Throughput Screen. 2003;6:257â66.Boroduleva AY, Wu J, Yang Q, Li H, Zhang Q, Li P, et al. Development of fluorescence polarization immunoassays for parallel detection of pesticides carbaryl and triazophos in wheat grains. Anal Methods. 2017;9:6814â22.Pourfarzaneh M, White GW, Landon J, Smith DS. Cortisol directly determined in serum by fluoroimmunoassay with magnetizable solid phase. Clin Chem. 1980;26:730â3.Mi T, Liang X, Ding L, Zhang S, Eremin SA, Beier RC, et al. Development and optimization of a fluorescence polarization immunoassay for orbifloxacin in milk. Anal Methods. 2014;6:3849â57
Development of Competitive ELISA and CLEIA for Quantitative Analysis of Polymyxin B
Polymyxin B (PMB), a member of polypeptide antibiotics, is widely used for the treatment of infection in animals such as cattle, sheep, pigs, and chickens. However, it is toxic on the kidneys and nervous system, and polymyxin resistance is increasingly reported, which leaves a serious threat to human health. Therefore, it is essential to establish rapid methods for detecting PMB with high sensitivity and specificity. In this study, an anti-PMB polyclonal antibody (pAb) was obtained by immunizing New Zealand white rabbits with PMB conjugated with glycosylated bovine serum albumin (GBSA). Indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and indirect competitive chemiluminescent enzyme immunoassay (ic-CLEIA) were developed. Under the optimal conditions, inhibitory concentrations (IC50) of PMB were 257.1 ng/mL (ic-ELISA) and 250.8 ng/mL (ic-CLEIA); the limits of detection (LOD) were 17.4 ng/mL (ic-ELISA) and 14.5 ng/mL (ic-CLEIA), respectively. Cross-reactivity of the pAb toward polymyxin E (PME) was 257.1%, and no response was found with other antibiotics. The recovery rates in spiked meat samples were 77.4 similar to 106.1% (ic-ELISA) and 84.1 similar to 107.1% (ic-CLEIA), respectively.Polymyxin B (PMB), a member of polypeptide antibiotics, is widely used for the treatment of infection in animals such as cattle, sheep, pigs, and chickens. However, it is toxic on the kidneys and nervous system, and polymyxin resistance is increasingly reported, which leaves a serious threat to human health. Therefore, it is essential to establish rapid methods for detecting PMB with high sensitivity and specificity. In this study, an anti-PMB polyclonal antibody (pAb) was obtained by immunizing New Zealand white rabbits with PMB conjugated with glycosylated bovine serum albumin (GBSA). Indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and indirect competitive chemiluminescent enzyme immunoassay (ic-CLEIA) were developed. Under the optimal conditions, inhibitory concentrations (IC50) of PMB were 257.1 ng/mL (ic-ELISA) and 250.8 ng/mL (ic-CLEIA); the limits of detection (LOD) were 17.4 ng/mL (ic-ELISA) and 14.5 ng/mL (ic-CLEIA), respectively. Cross-reactivity of the pAb toward polymyxin E (PME) was 257.1%, and no response was found with other antibiotics. The recovery rates in spiked meat samples were 77.4 similar to 106.1% (ic-ELISA) and 84.1 similar to 107.1% (ic-CLEIA), respectively
Quantification of Thiram in Honeybees: Development of a Chemiluminescent ELISA
International audienceA Chemiluminescence Enzyme-Linked Immuno-Sorbent Assay (CL-ELISA) for determination and quantification of the fungicide thiram in honeybees was developed in an indirect competitive format. The assay was optimized by determining: the optimal coating conjugate concentration and anti-thiram antiserum dilution, the effect of the incubation time on the competitive step, the tolerance to organic solvents. The IC50 and the limit of detection (LOD) values were 60 ng mL-1 and 9 ng mL -1, respectively, similar to those of colorimetric ELISA with a calibration range of 9 â 15,000 ng mL-1. Cross reactivity of some related compounds such as some dithiocarbamates, a thiocarbamate, the ethylenethiourea and the tetramethylthiourea were tested. The assay was then applied to honeybees sample extracts obtained by using the liquid-liquid extraction or the graphitized carbon-based solid phase extraction. The calibration curves in honeybee extracts from liquid-liquid procedure gave an IC50 of 141 ng mL-1 and a LOD of 17 ng mL-1. In case of extracts obtained by SPE these values were 139 ng mL-1 and 15 ng mL-1, respectively. The average recovery value from honeybee extracts spiked with 75 ng mL-1 of thiram was 72% for SPE, higher than for liquid-liquid extraction (60%). On the opposite, when the honeybees were directly spiked with 2 and 10 ppm the average recovery was higher for liquid-liquid extraction (54%), than for SPE (31%). Finally, the assay was applied to honeybee samples collected during monitoring activities in Italy and Russia
Exploring the antiâαâamylase activity of flavonoid aglycones in fabaceae plant extracts: a combined MALDIâTOFâMS and LCâMS/MS approach
A combination of TLC-bioautography, MALDI-TOF-MS and LCâMS/MS methods was used to identify flavonoids with anti-α-amylase activity in extracts of Lathyrus pratensis L. (herb), L.âpolyphillus L. (fruits), Thermopsisâlanceolata R. Br. (herb) and S.âjaponica L. (buds). After the TLC-autobiography assay, substances with anti-amylase activity were identified by MALDI-TOF-MS followed by confirmation of the result by LCâMS/MS. Results of the study revealed that the flavonoids apigenin, luteolin, formononetin, genistein and kaempferol display marked anti-α-amylase activity. Formononetin showed the largest activity. Compared with LCâMS/MS, MALDI-TOF-MS is a quick and convenient method; results can be obtained within minutes; and only minor sample amounts are required which allows us to analyse mixtures of substances without preliminary separation. However, the inability to distinguish between isomers is the main limitation of the method.BAM/BMWi
http://dx.doi.org/10.13039/501100002765German Research Foundation
http://dx.doi.org/10.13039/501100001659Peer Reviewe
Recommended from our members
EPMA-World Congress 2015: Bonn, Germany. 3-5 September 2015
Table of contents A1 Predictive and prognostic biomarker panel for targeted application of radioembolisation improving individual outcomes in hepatocellular carcinoma Jella-Andrea Abraham, Olga Golubnitschaja A2 Integrated market access approach amplifying value of âRx-CDxâ Ildar Akhmetov A3 Disaster response: an opportunity to improve global healthcare Russell J. Andrews, Leonidas Quintana A4 USA PPPM: proscriptive, profligate, profiteering medicine-good for 1 % wealthy, not for 99 % unhealthy Russell J. Andrews A5 The role of IDO in a murine model of gingivitis: predictive and therapeutic potentials Babak Baban, Jun Yao Liu, Xu Qin, Tailing Wang, Mahmood S. Mozaffari A6 Specific diets for personalised treatment of diabetes type 2 Viktoriia V. Bati, Tamara V. Meleshko, Olga B. Levchuk, Nadiya V. Boyko A7 Towards personalized physiotherapeutic approach Joanna Bauer, Ewa Boerner, Halina Podbielska A8 Cells, animal, SHIME and in silico models for detection and verification of specific biomarkers of non-communicable chronic diseases Alojz Bomba, Viktor O. Petrov, Volodymyr G. Drobnych, Rostyslav V. Bubnov, Oksana M. Bykova, Nadiya V. Boyko A9 INTERACT-chronic care model: Self-treatment by patients with decision support e-Health solution Hans-Peter Brunner-La Rocca, Lutz Fleischhacker, Olga Golubnitschaja, Frank Heemskerk, Thomas Helms, Tiny Jaarsma, Judita Kinkorova, Jan Ramaekers, Peter Ruff, Ivana Schnur, Emilio Vanoli, Jose Verdu A10 PPPM in cardiovascular medicine in 2015 Hans-Peter Brunner-La Rocca A11 Magnetic resonance imaging of nanoparticles in mice, potential for theranostic and contrast media development â pilot results Rostyslav V. Bubnov, Sergiy A. Grabovetskyi, Olena M. Mykhalchenko, Natalia O. Tymoshok, Oleksandr B. Shcherbakov, Igor P. Semeniv, Mykola Y. Spivak A12 Ultrasound diagnosis for diabetic neuropathy - comparative study Rostyslav V. Bubnov, Tetyana V. Ostapenko A13 Ultrasound for stratification patients with diabetic foot ulcers for prevention and personalized treatment - pilot results Rostyslav V. Bubnov, Nazarii M. Kobyliak, Nadiya M. Zholobak, Mykola Ya. Spivak A14 Project ImaGenX â designing and executing a questionnaire on environment and lifestyle risk of breast cancer John Paul Cauchi A15 Genomics â a new structural brand of predictive, preventive and personalized medicine or the new driver as well? Dmitrii Cherepakhin, Marina Bakay, Artem Borovikov, Sergey Suchkov A16 Survey of questionnaires for evaluation of the quality of life in various medical fields Barbara CieĆlik, Agnieszka Migasiewicz, Maria-Luiza Podbielska, Markus Pelleter, Agnieszka Giemza, Halina Podbielska A17 Personalized molecular treatment for muscular dystrophies Sebahattin Cirak A18 Secondary mutations in circulating tumour DNA for acquired drug resistance in patients with advanced ALK + NSCLC Marzia Del Re, Paola Bordi, Valentina Citi, Marta Palombi, Carmine Pinto, Marcello Tiseo, Romano Danesi A19 Recombinant species-specific FcΔRI alpha proteins for diagnosis of IgE-mediated allergies in dogs, cats and horses Lukas Einhorn, Judit Fazekas, Martina Muhr, Alexandra Schoos, Lucia Panakova, Ina Herrmann, Krisztina Manzano-Szalai, Kumiko Oida, Edda Fiebiger, Josef Singer, Erika Jensen-Jarolim A20 Global methodology for developmental neurotoxicity testing in humans and animals early and chronically exposed to chemical contaminants ArpinĂ© A. Elnar, Nadia Ouamara, Nadiya Boyko, Xavier Coumoul, Jean-Philippe Antignac, Bruno Le Bizec, Gauthier Eppe, Jenny Renaut, Torsten Bonn, CĂ©dric Guignard, Margherita Ferrante, Maria Liusa Chiusano, Salvatore Cuzzocrea, Gerard O'Keeffe, John Cryan, Michelle Bisson, Amina Barakat, Ihsane Hmamouchi, Nasser Zawia, Anumantha Kanthasamy, Glen E. Kisby, Rui Alves, Oscar Villacañas PĂ©rez, Kim Burgard, Peter Spencer, Norbert Bomba, Martin Haranta, Nina Zaitseva, Irina May, StĂ©phanie Grojean, Mathilde Body-Malapel, Florencia Harari, Raul Harari, Kristina Yeghiazaryan, Olga Golubnitschaja, Vittorio Calabrese, Christophe Nemos, Rachid Soulimani A21 Mental indicators at young people with attributes hypertension and pre-hypertension Maria E. Evsevyeva, Elena A. Mishenko, Zurida V. Kumukova, Evgeniy V. Chudnovsky, Tatyana A. Smirnova A22 On the approaches to the early diagnosis of stress-induced hypertension in young employees of State law enforcement agencies Maria E. Evsevyeva, Ludmila V. Ivanova, Michail V. Eremin, Maria V. Rostovtseva A23 ĐĄentral aortic pressure and indexes of augmentation in young persons in view of risk factors Maria E. Evsevyeva, Michail V. Eremin, Vladimir I. Koshel, Oksana V. Sergeeva, Nadesgda M. Konovalova A24 Breast cancer prediction and prevention: Are reliable biomarkers in horizon? Shantanu Girotra, Olga Golubnitschaja A25 Flammer Syndrome and potential formation of pre-metastatic niches: A multi-centred study on phenotyping, patient stratification, prediction and potential prevention of aggressive breast cancer and metastatic disease Olga Golubnitschaja, Manuel Debald, Walther Kuhn, Kristina Yeghiazaryan, Rostyslav V. Bubnov, Vadym M. Goncharenko, Ulyana Lushchyk, Godfrey Grech, Katarzyna Konieczka A26 Innovative tools for prenatal diagnostics and monitoring: improving individual pregnancy outcomes and health-economy in EU Olga Golubnitschaja, Jan Jaap Erwich, Vincenzo Costigliola, Kristina Yeghiazaryan, Ulrich Gembruch A27 Immunohistochemical assessment of APUD cells in endometriosis Vadym M. Goncharenko, Vasyl O. Beniuk, Olga V. Kalenska, Rostyslav V. Bubnov A28 Updating personalized management algorithm of endometrial hyperplasia in pre-menopause women Vadym M. Goncharenko, Vasyl O. Beniuk, Rostyslav V. Bubnov, Olga Melnychuk A29 The personified treatment approach of polimorbid patients with periodontal inflammatory diseases Irina A. Gorbacheva, Lyudmila Y. Orekhova, Vadim V. Tachalov A30 Ukrainian experience in hybrid war â the challenge to update algorithms for personalized care and early prevention of different military injuries Olena I. Grechanyk, Rizvan Ya. Abdullaiev, Rostyslav V. Bubnov A31 Tear fluid biomarkers: a comparison of tear fluid sampling and storage protocols Suzanne Hagan, Eilidh Martin, Ian Pearce, Katherine Oliver A32 The correlation of dietary habits with gingival problems during menstruation Cenk Haytac, Fariz Salimov, Servin Yoksul, Anatoly A. Kunin, Natalia S. Moiseeva A33 Genomic medicine in a contemporary Spanish population of prostate cancer: our experience Bernardo Herrera-Imbroda, Sergio del RĂo-GonzĂĄlez, Maria Fernanda Lara, Antonia Angulo, Francisco Javier Machuca Santa-Cruz A34 Challenges, opportunities and collaborations for personalized medicine applicability in uro-oncological disease Bernardo Herrera-Imbroda, Sergio del RĂo-GonzĂĄlez, Maria Fernanda Lara A35 Metabolic hallmarks of cancer as targets for a personalized therapy John Ionescu A36 Influence of genetic polymorphism as a predictor of the development of periodontal disease in patients with gastric ulcer and 12 duodenal ulcer Alfiya Z. Isamulaeva, Anatoly A. Kunin, Shamil Sh. Magomedov, Aida I. Isamulaeva A37 Challenges in diabetic macular edema Tatjana Josifova A38 Overview of the EPMA strategies in laboratory medicine relevant for PPPM Marko Kapalla, Juraj KubĂĄĆ, Olga Golubnitschaja, Vincenzo Costigliola A39 EPMA initiative for effective organization of medical travel: European concepts and criteria Vincenzo Costigliola, Marko Kapalla, Juraj KubĂĄĆ, Olga Golubnitschaja A40 Design and innovation in e-textiles: implications for PPPM Anthony Kent, Tom Fisher, Tilak Dias A41 Biobank in Pilsen as a member of national node BBMRI_CZ Judita KinkorovĂĄ, OndĆej TopolÄan A42 Big data in personalized medicine: hype and hope Matthias Kohl A43 The 3P approach as the platform of the European Dentistry Department (DPPPD) Anatoly A. Kunin, Natalia S. Moiseeva A44 The endometrium cytokine patterns for predictive diagnosis of proliferation severity and cancer prevention Andrii I. Kurchenko, Vasyl A. Beniuk, Vadym M. Goncharenko, Rostyslav V. Bubnov, Nadiya V. Boyko, Andriy M. Strokan A45 A monocyte-based in-vitro system for testing individual responses to the implanted material: future for personalized implant construction Julia Kzhyshkowska, Alexandru Gudima, Ksenia S. Stankevich, Victor D. Filimonov4, Harald KlĂŒter, Evgeniya M. Mamontova, Sergei I. Tverdokhlebov A46 Prediction and prevention of adverse health effects by meteorological factors: Biomarker patterns and creation of a device for self-monitoring and integrated care Ulyana B. Lushchyk, Viktor V. Novytskyy, Igor P. Babii, Nadiya G. Lushchyk, Lyudmyla S. Riabets, Ivanna I. Legka A47 Targeting "disease signatures" towards personalized healthcare Mira Marcus-Kalish, Alexis Mitelpunkt, Tal Galili, Neta Shachar, Yoav Benjamini A48 Influence of the skin imperfection on the personal quality of life and possible tools for objective diagnosis Agnieszka Migasiewicz, Markus Pelleter, Joanna Bauer, Ewelina DereĆ, Halina Podbielska A49 The new direction in caries prevention based on the ultrastructure of dental hard tissues and filling materials Natalia S. Moiseeva, Anatoly A. Kunin, Dmitry A. Kunin A50 The use of LED radiation in prevention of dental diseases Natalia S. Moiseeva, Yury A. Ippolitov, Dmitry A. Kunin, Alexei N. Morozov, Natalia V. Chirkova, Nakhid T. Aliev A51 Status of endothelial progenitor cells in diabetic nephropathy: predictive and preventive potentials Mahmood S. Mozaffari, Jun Yao Liu, Babak Baban A52 The status of glucocorticoid-induced leucine zipper protein in salivary gland in Sjögrenâs syndrome: predictive and personalized treatment potentials Mahmood S. Mozaffari, Jun Yao Liu, Rafik Abdelsayed, Xing-Ming Shi, Babak Baban A53 Maximal aerobic capacity - important quality marker of health Jaroslav NovĂĄk, Milan Ć tork, VĂĄclav Zeman A54 The EMPOWER project: laboratory medicine and Horizon 2020 Wytze P. Oosterhuis, Elvar Theodorsson A55 Personality profile manifestations in patientâs attitude to oral care and adherence to doctorâs prescriptions Lyudmila Y. Orekhova, Tatyana V. Kudryavtseva, Elena R. Isaeva, Vadim V. Tachalov, Ekaterina S. Loboda A56 Results of an European survey on personalized medicine addressed to directions of laboratory medicine Mario Pazzagli, Francesca Malentacchi, Irene Mancini, Ivan Brandslund, Pieter Vermeersch, Matthias Schwab, Janja Marc, Ron H.N. van Schaik, Gerard Siest, Elvar Theodorsson, Chiara Di Resta A57 MCI or early dementia predictive speech based diagnosis techniques Matus Pleva, Jozef Juhar A58 Personalized speech based mobile application for eHealth Matus Pleva, Jozef Juhar A59 Circulating tumor cell-free DNA as the biomarker in the management of cancer patients JiĆĂ PolĂvka jr., Filip JankĆŻ, Martin PeĆĄta, Jan DoleĆŸal, Milena KrĂĄlĂÄkovĂĄ, JiĆĂ PolĂvka A60 Complex stroke care â educational programme in Stroke Centre University Hospital Plzen JiĆĂ PolĂvka, Alena LukeĆĄovĂĄ, Nina MĂŒllerovĂĄ, Petr Ć evÄĂk, VladimĂr Rohan A61 Sleep apnea and sleep fragmentation contribute to brain aging Kneginja Richter, Lence Miloseva, GĂŒnter Niklewski A62 Personalised approach for sleep disturbances in shift workers Kneginja Richter, Jens Acker, Guenter Niklewski A63 Medical travel and innovative PPPM clusters: new concept of integration Olga Safonicheva, Vincenzo Costigliola A64 Medical travel and women health Olga Safonicheva A65 Continuity of generations in the training of specialists in the field of reconstructive microsurgery Maxim Sautin, Janna Sinelnikova, Sergey Suchkov A66 Telemonitoring of stroke patients â empirical evidence of individual risk management results from an observational study in Germany SongĂŒl Secer, Stephan von Bandemer A67 Womenâs increasing breast cancer risk with n-6 fatty acid intake explained by estrogen-fatty acid interactive effect on DNA damage: implications for gender-specific nutrition within personalized medicine Niva Shapira A68 Cytobacterioscopy of the gingival crevicular fluid as a method for preventive diagnosis of periodontal diseases Aleksandr Shcherbakov, Anatoly A. Kunin, Natalia S. Moiseeva A69 Use of specially treated composites in dentistry to avoid violations of aesthetics Bogdan R. Shumilovich, Zhanna Lipkind, Yulia Vorobieva, Dmitry A. Kunin, Anastasiia V. Sudareva A70 National eHealth system â platform for preventive, predictive and personalized diabetes care Ivica Smokovski, Tatjana Milenkovic A72 The common energy levels of Prof. Szent-Györgyi, the intrinsic chemistry of melanin, and the muscle physiopathology. Implications in the context of Preventive, Predictive, and Personalized Medicine Arturo SolĂs-Herrera, MarĂa del Carmen Arias-Esparza, Sergey Suchkov A73 Plurality and individuality of hepatocellular carcinoma: PPPM perspectives Krishna Chander Sridhar, Olga Golubnitschaja A74 Strategic aspects of higher medical education reforms to secure newer educational platforms for getting biopharma professionals matures Maria Studneva, Sihong Song, James Creeden, Đark Đandrik, Sergey Suchkov A75 Overview of the strategies and activities of the European Federation of Clinical Chemistry and Laboratory Medicine, (EFLM) Elvar Theodorsson, EFLM A76 New spectroscopic techniques for point of care label free diagnostics Syed A. M. Tofail A77 Tumor markers for personalized medicine and oncology - the role of Laboratory Medicine OndĆej TopolÄan, Judita KinkorovĂĄ, OndĆej Fiala, Marie KarlĂkovĂĄ, Ć ĂĄrka SvobodovĂĄ, Radek KuÄera, Radka FuchsovĂĄ, Vladislav TĆeĆĄka, VĂĄclav Ć imĂĄnek, Ladislav Pecen, Jan Ć oupal, Ć tÄpĂĄn SvaÄina2 A78 Modern medical terminology (MMT) as a driver of the global educational reforms Evgeniya Tretyak, Maria Studneva, Sergey Suchkov A79 Juvenile hypertension; the relevance of novel predictive, preventive and personalized assessment of its determinants Francesca M. Trovato, G. Fabio Martines, Daniela Brischetto, Daniela Catalano, Giuseppe Musumeci, Guglielmo M. Trovato A80 Proteomarkers Biotech George Th. Tsangaris, Athanasios K. Anagnostopoulos A81 Proteomics and mass spectrometry based non-invasive prenatal testing of fetal health and pregnancy complications George Th. Tsangaris, Athanasios K. Anagnostopoulos A82 Integrated Ecosystem for an Integrated Care model for Heart Failure (HF) patients including related comorbidities (ZENITH) JosĂ© VerdĂș, German GutiĂ©rrez, Jordi Rovira, Marta Martinez, Lutz Fleischhacker, Donna Green, Arthur Garson, Elena Tamburini, Stefano Cuomo, Juan Martinez-Leon, Teresa Abrisqueta, Hans-Peter Brunner-La Rocca, Tiny Jaarsma, Teresa Arredondo, Cecilia Vera, Giuseppe Fico, Olga Golubnitschaja, Fernando Arribas, Martina Onderco, Isabel Vara, on behalf of ZENITH consortium A83 Predictive, preventive and personalized medicine in diabetes onset and complication (MOSAIC project) JosĂ© VerdĂș, Francesco Sambo, Barbara Di Camillo, Claudio Cobelli, Andrea Facchinetti, Giuseppe Fico, Riccardo Bellazzi, Lucia Sacchi, Arianna Dagliati, Daniele Segnani, Valentina Tibollo, Manuel Ottaviano, Rafael Gabriel, Leif Groop, Jacqueline Postma, Antonio Martinez, Liisa Hakaste, Tiinamaija Tuomi, Konstantia Zarkogianni, on behalf of MOSAIC consortium A84 Possibilities for personalized therapy of diabetes using in vitro screening of insulin and oral hypoglycemic agents Igor Volchek, Nina Pototskaya, Andrey Petrov A85 The innovative technology for personalized therapy of human diseases based on in vitro drug screening Igor Volchek, Nadezhda Pototskaya, Andrey Petrov A86 Bone destruction and temporomandibular joint: predictive markers, pathogenetic aspects and quality of life Ălle Voog-Oras, Oksana Jagur, Edvitar Leibur, Priit Niibo, Triin JagomĂ€gi, Minh Son Nguyen, Chris Pruunsild, Dagmar Piikov, Mare Saag A87 Sub-optimal health management â global vision for concepts in medical travel Wei Wang A88 Sub-optimal health management: synergic PPPM-TCAM approach Wei Wang A89 Innovative technologies for minimal invasive diagnostics Andreas WeinhĂ€usel, Walter Pulverer, Matthias Wielscher, Manuela Hofner, Christa Noehammer, Regina Soldo, Peter Hettegger, Istvan Gyurjan, Ronald Kulovics, Silvia Schönthaler, Gabriel Beikircher, Albert Kriegner, Stephan Pabinger, Klemens Vierlinger A90 Rare disease diobanks for personalized medicine AyĆe YĂŒzbaĆıoÄlu, Meral ĂzgĂŒĂ§, Member of EuroBioBank - European Network of DNA, Cell and Tissue Banks for Rare Disease
Express Detection of Pentachlorophenol as Dioxins Precursor in Natural Water
A rapid detection method for the pesticide pentachlorophenol (PCP) â polarization fluoroimmunoassay (PFIA) â in the dynamic range of 10â9,000 ppb was developed. PCP may form polychlorinated dibenzo-p-dioxins, making environmental monitoring of this compound an issue of great importance. In order to optimize the PFIA procedure, a number of fluorescein-labeled PCP derivatives and similar compounds (tracers) were synthesized, and the influence of their structure on PFIA characteristics was studied. Also, two antisera were tested in developing PFIA for PCP. The developed method is highly specific for PCP and can be used for its determination in water samples at a level down to 10 ppb. Total time of the assay for 10 samples is about 7 min. The assay provides a useful and a highly practical screening tool for the processing of large numbers of samples and for the preliminary estimation of potential dioxins contamination in water resources
Hematological parameters that determine the course of labor and the postpartum period in highly productive Holstein cows
The aim of the study is to determine the effect of hematological parameters of cows on the course of labor and the postpartum period in highly productive Holstein cows. For this purpose, one group of cows was formed on the principle of analogue pairs in the amount of 30 animals with a gestation period of 7.5â8.0 months, followed by blood sampling from them using the Monovet system in vacuum test tubescontainers to determine morphological and biochemical parameters. Blood was taken 25â30 days before delivery in the morning 2 hours before feeding. Blood values were studied according to generally accepted methods using certified equipment. Subsequently, depending on the nature of the course of the birth, the animals were divided into two groups. The first group included 24 cows without pathology of the course of labor and the postpartum period, the second group included 16 cows with pathologies of the course of labor and the postpartum period. In the process of research, it was found that in 60.0 % of the cows the birth and the postpartum period passed without pathologies, and in 40.0 % of the birth and postpartum complications were revealed (retention of the placenta, acute postpartum endometritis, uterine subinvolution). A comparative analysis of the blood values of cows 25â30 days before birth the course of the labor process and postpartum period showed that in animals without pathology â compared to the animals with pathologies â the hematological parameters are considerably higher (hemoglobin content by 16.42 g/l, total protein by 10.96 g/l, albumin by 7.7 %, calcium by 0.98 mmol/l, glucose by 0.91 mmol, immunoglobin A by 59.77 mg/M), while the content of beta-globulins is lower by 8.51 % and that of enzyme AcT is lower by 25.94 units/l. A decrease in blood biochemical parameters contributes to the manifestation of the pathology of labor and the postpartum period. The data obtained can be used to develop an algorithm for the prevention of postpartum complications in highly productive cows
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