Mycobacterium tuberculosis Beijing genotype induces differential cytokine production by peripheral blood mononuclear cells of healthy BCG vaccinated individuals.

Contains fulltext : 124298.pdf (publisher's version ) (Closed access)Members of the Mycobacterium tuberculosis (Mtb) Beijing genotype are a major concern due to their high prevalence in tuberculosis patients and their high rate of multi-drug resistance. Although it has been shown that Beijing modifies macrophage behavior, little is known about how this genotype could affect the cellular immune response. In order to address this issue, peripheral blood mononuclear cells (PBMC) from healthy BCG vaccinated individuals were stimulated with protein extracts from three Mycobacterium tuberculosis genotypes: Canetti, H37Rv and Beijing evaluating T cell proliferation and cytokine production. In this system both CD4+ and CD8+ proliferated in a similar manner independently of the Mtb genotype used for stimulation. Regarding cytokines, all strains induced similar levels of IFN-gamma, but were unable to induce IL-4 and TGF-beta. Contrasting, Canetti strain induced lower production of IL-10, TNF-alpha and IL-12 compared to H37Rv and Beijing. Interestingly, PBMC stimulated with the Beijing strain produced the highest levels of IL-12 and IL-10 than those stimulated with other strains. This differential cytokine expression could affect the pathogenesis induced by Beijing strain through the modulation of inflammatory process in the host, but the precise mechanisms by which this cytokine environment affects the Beijing strain pathogenesis needs further characterization

Segmented political assimilation: perceptions of racialized opportunities and Latino immigrants' partisan identification

To account for Latino immigrants' assimilation into the American political mainstream, I derive social psychological factors from the contextual notion of 'modes of incorporation' in the segmented assimilation literature. These social psychological factors, perceptions of racialized opportunities (PROPs), relate to immigrants' adoption of political party identities (i.e. Democrat, Republican). I test these PROPs factors utilizing the 2006 Latino National Survey (N=5,717 immigrant Latino respondents). Multinomial logistic regressions predicting party identification, compared to either 'Don't Know' or 'Don't Care' options, indicate that PROPs are significantly related to Latino immigrants' identification as either Democrats or Republicans. High levels of identification with perceived white opportunities are related to Republican identity and high levels of identification with perceived black opportunities differentiate Democrats from Republicans