Prefoldin 6 mediates longevity response from heat shock factor 1 to FOXO in C-elegans

Heat shock factor 1 (HSF-1) and forkhead box O (FOXO) are key transcription factors that protect cells from various stresses. In Caenorhabditis elegans, HSF-1 and FOXO together promote a long life span when insulin/IGF-1 signaling (IIS) is reduced. However, it remains poorly understood how HSF-1 and FOXO cooperate to confer IIS-mediated longevity. Here, we show that prefoldin 6 (PFD-6), a component of the molecular chaperone prefoldin-like complex, relays longevity response from HSF-1 to FOXO under reduced IIS. We found that PFD-6 was specifically required for reduced IIS-mediated longevity by acting in the intestine and hypodermis. We showed that HSF-1 increased the levels of PFD-6 proteins, which in turn directly bound FOXO and enhanced its transcriptional activity. Our work suggests that the prefoldin-like chaperone complex mediates longevity response from HSF-1 to FOXO to increase the life span in animals with reduced IIS.11Ysciescopu

Calibration of TRACE Lyman-

1216 Å  channel images from the Transition Region and Coronal Explorer (TRACE) contain not only the desired HI Lyman-α (Lα) line emission from the Sun, but also some UV continuum around 1600 Å. By comparing TRACE data with Lα images from the Very High Angular Resolution Ultraviolet Telescope, Handy et al. proposed a simple procedure to remove the contamination: a linear combination of 1216 Å  and 1600 Å  channel images from the TRACE. In order to check the reliability of this procedure, here we compare TRACE data with Lα raster scan images from the SUMER instrument onboard the Solar and Heliospheric Observatory (SOHO). We obtain a linear combination coefficient very similar to that of Handy et al., and also find that the apparent spatial resolution of the Lα raster scan images from SOHO/SUMER is at least 4 \arcsec \times 4 \arcsec, which is more than 2–4 times lower than that of its single spectrum

Anti-allergic inflammatory effect of vanillic acid through regulating thymic stromal lymphopoietin secretion from activated mast cells

<p>Vanillic acid, which is well known as a benzoic acid derivative, has been used as a flavouring agent. Currently, we ascertained the therapeutic potential action of vanillic acid on allergic inflammatory reaction in human mast cell line, HMC-1. Treatment with vanillic acid resulted in a significant decrease in levels of thymic stromal lymphopoietin and pro-inflammatory cytokines compared to phorbol 12-myristate 13-acetate and calcium ionophore A23187 (PMACI)-treated HMC-1 cells. In PMACI-stimulated cells, treatment with vanillic acid also dramatically inhibited activities of caspase-1 and nuclear factor-kB (p65). Furthermore, treatment with vanillic acid suppressed phosphorylation of mitogen-activated protein kinases in PMACI-treated HMC-1 cells. Taken together, these findings suggest that vanillic acid has a beneficial effect on allergic inflammatory disorders.</p

Tumor Mesenchymal Stem-Like Cell as a Prognostic Marker in Primary Glioblastoma

The isolation from brain tumors of tumor mesenchymal stem-like cells (tMSLCs) suggests that these cells play a role in creating a microenvironment for tumor initiation and progression. The clinical characteristics of patients with primary glioblastoma (pGBM) positive for tMSLCs have not been determined. This study analyzed samples from 82 patients with pGBM who had undergone tumor removal, pathological diagnosis, and isolation of tMSLC from April 2009 to October 2014. Survival, extent of resection, molecular markers, and tMSLC culture results were statistically evaluated. Median overall survival was 18.6 months, 15.0 months in tMSLC-positive patients and 29.5 months in tMSLC-negative patients (P=0.014). Multivariate cox regression model showed isolation of tMSLC (OR = 2.5, 95% CI = 1.1~5.6, P=0.021) showed poor outcome while larger extent of resection (OR = 0.5, 95% CI = 0.2~0.8, P=0.011) has association with better outcome. The presence of tMSLCs isolated from the specimen of pGBM is associated with the survival of patient

Stimulation of the Extracellular Matrix Production in Dermal Fibroblasts by Velvet Antler Extract

고, RT-PCR에서 는 COL1A1, TGF-β1, keratinocyte growth factor (KGF), insulin growth factor (IGF)-1 의 유전 자 발현 을 증가시켰다. 이상 의 결과로부 터 대복피추출물 은 진피섬유모세포에 서 세포외기질 의 생성 을 촉진시키 는 천연소재인 것으 로 판단되었다. Abstract Dermal fibroblasts produce the many components of the extracellular matrix (ECM) that are needed to maintain connective tissue integrity and repair tissue injuries. This study investigated the effects of Areca catechu extract (ACE) on dermal fibroblast cell activation. Cultured human dermal fibroblasts were treated with ACE, and then ECM production was determined by ELISA, Western blot and RT-PCR. ACE significantly accelerated the production of type 1 collagen, fibronectin, and transforming growth factor (TGF)-β1 by ELISA and type 1 collagen by Western blot assay. ACE also increased the gene expression of COL1A1, TGF-β1, keratinocyte growth factor (KGF) and insulin growth factor (IGF)-1. These results suggest that ACE has th