The influence of the food environment on overweight and obesity in young children : a systematic review

The increasing prevalence of childhood obesity has led to interest in its prevention, particularly through school-based and family-based interventions in the early years. Most evidence reviews, to date, have focused on individual behaviour change rather than the ‘obesogenic environment’. This paper reviews the evidence on the influence of the food environment on overweight and obesity in children up to 8 years. Electronic databases (including MEDLINE, EMBASE, Cochrane Controlled Trials Register (CCTR), DARE, CINAHL and Psycho-Info) and reference lists of original studies and reviews were searched for all papers published up to 31 August 2011. Study designs included were either population-based intervention studies or a longitudinal study. Studies were included if the majority of the children studied were under 9 years, if they related to diet and if they focused on prevention rather than treatment in clinical settings. Data included in the tables were characteristics of participants, aim, and key outcome results. Quality assessment of the selected studies was carried out to identify potential bias and an evidence ranking exercise carried out to prioritise areas for future public health interventions. Thirty-five studies (twenty-five intervention studies and ten longitudinal studies) were selected for the review. There was moderately strong evidence to support interventions on food promotion, large portion sizes and sugar-sweetened soft drinks. Reducing food promotion to young children, increasing the availability of smaller portions and providing alternatives to sugar-sweetened soft drinks should be considered in obesity prevention programmes aimed at younger children. These environment-level interventions would support individual and family-level behaviour change

Polyploidy, infraspecific cytotype variation, and speciation in Goldenrods: The cytogeography of Solidago subsect. Humiles (Asteraceae) in North America

Polyploidy is an important evolutionary mechanism in plants, and in some genera (e.g., Solidago in Asteraceae) it is particularly widespread and is hypothesized to have played a major role in diversification. Goldenrods are notorious for their ploidy variation, with roughly 14% and 32% of recognized North American species being polyploid or including multiple cytotypes, respectively. We used traditional chromosome counts and flow cytometry to examine cytogeographic patterns, biogeographic and evolutionary hypotheses, and species boundaries in S. subsect. Humiles. Chromosome numbers and DNA ploidy determinations are reported for 337 individuals, including 148 new reports. Cytotypes show significant geographic structuring. Solidago simplex and S. spathulata were uniformly diploid (2n = 18) in western North America, while cytogeographic patterns in eastern North America were regionally complex and included 2n, 4n, and 6n cytotypes. Cytotypes within S. simplex were ecogeographically segregated and mixed‐ploidy populations were rare. Data from this study and additional biosystematic data indicate that cytotypes in S. simplex fulfill the requirements of multiple species concepts and should best be treated as distinct species. Polyploid cytotypes possibly formed recurrently, however, and evolution and species boundaries within poly ploid S. simplex will require additional study. Results from this study and accumulated data from other studies suggest that biological species diversity in Solidago is considerably higher than currently recognized taxonomically.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/147108/1/tax611014-sup-001-PDF.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/147108/2/tax611014.pd

Arranged marriages, broken families and concerned parents : a "bounded choice" perspective on commitment in Grace Gospel Church.

Thesis (M.Th.)-University of KwaZulu-Natal, Pietermaritzburg, 2013.Grace Gospel Church (GGC) is a small congregation that has met in various venues in Pinetown, KwaZulu-Natal since 2003. It is a partner church of the Mauritian-based organisation, Church Team Ministries International (CTMI), led by Miki Hardy. Hardy is a pastor who claims to have received a unique revelation from God, and he is considered to be an apostle by his followers. Grace Gospel Church and Church Team Ministries International have gained international attention due to claims that they exert a controversial influence over their members. This influence appears to induce a radical commitment to the partner church and CTMI, leading to behavioural, career and study changes, arranged marriages and estrangement between members and their families. Grace Gospel Church has been accused of being a cult, and this exploratory research questions whether this accusation has justification by comparing the group’s theology and practices against theological, psychological and sociological research on cults. Cult researcher Janja Lalich’s theory of “bounded choice” is described and used to evaluate the commitment witnessed in GGC. Bounded choice exists when, as a result of psycho-social group dynamics, a personal fusion occurs between the individual and the ideology and mores of the group. Out of this fusion, a charismatically committed “true believer” emerges who may appear to have personal freedom, but whose palette of choices is severely curtailed by the beliefs and practices of the group. “Bounded” decision making results which might appear irrational or repugnant to those outside the group, but to true believers represent their highest aspirations. This research indicates that GGC shares many characteristic features of typical cults and also promotes the conditions necessary for bounded choice to occur. It is thus concluded that GGC could legitimately be termed a cult, and that bounded choice is a valid explanation for the controversial choices made by its members

Location Coding by Opponent Neural Populations in the Auditory Cortex

Although the auditory cortex plays a necessary role in sound localization, physiological investigations in the cortex reveal inhomogeneous sampling of auditory space that is difficult to reconcile with localization behavior under the assumption of local spatial coding. Most neurons respond maximally to sounds located far to the left or right side, with few neurons tuned to the frontal midline. Paradoxically, psychophysical studies show optimal spatial acuity across the frontal midline. In this paper, we revisit the problem of inhomogeneous spatial sampling in three fields of cat auditory cortex. In each field, we confirm that neural responses tend to be greatest for lateral positions, but show the greatest modulation for near-midline source locations. Moreover, identification of source locations based on cortical responses shows sharp discrimination of left from right but relatively inaccurate discrimination of locations within each half of space. Motivated by these findings, we explore an opponent-process theory in which sound-source locations are represented by differences in the activity of two broadly tuned channels formed by contra- and ipsilaterally preferring neurons. Finally, we demonstrate a simple model, based on spike-count differences across cortical populations, that provides bias-free, level-invariant localization—and thus also a solution to the “binding problem” of associating spatial information with other nonspatial attributes of sounds

Assessing progress in protecting non-smokers from secondhand smoke

Objective To examine trends in population exposure to second-hand tobacco smoke (SHS) and consider two exposure metrics as appropriate targets for tobacco control policymakers. Design Comparison of adult non-smokers’ salivary cotinine data available from eleven Scottish Health Surveys between 1998 and 2016. Methods The proportions of non-smoking adults who had measurable levels of cotinine in their saliva were calculated for the eleven time points. The Geometric Mean (GM) concentrations of cotinine levels were calculated using Tobit regression. Changes in both parameters were assessed for the whole period and also for the years since implementation of smoke-free legislation in Scotland in 2006. Results Salivary cotinine expressed as a GM fell from 0.464 ng/ml (95% CI 0.444-0.486 ng/ml) in 1998 to 0.013 ng/ml (95% CI 0.009-0.020 ng/ml) in 2016: a reduction of 97.2%. The percentage of non-smoking adults who had no measurable cotinine in their saliva increased by nearly six-fold between 1998 (12.5% [95% CI 11.5%-13.6%]) and 2016 (81.6% [95% CI 78.6%-84.6%]). Reductions in population exposure to SHS have continued even after smoke-free legislation in 2006. Conclusions Scotland has witnessed a dramatic reduction in SHS exposure in the past two decades but there are still nearly one in five non-smoking adults who have measurable exposure to SHS on any given day. Tobacco control strategies globally should consider the use of both the proportion of non-smoking adults with undetectable salivary cotinine and the GM as targets to encourage policies that achieve a smoke-free future

Distinct phenotypes of platelet, monocyte, and neutrophil activation occur during the acute and convalescent phase of COVID-19

SARS-CoV-2 has spread rapidly worldwide, causing the COVID-19 pandemic. Platelet activation and platelet-leukocyte complex formation are proposed to contribute to disease progression. Here, we report platelet and leukocyte activation during acute and convalescent COVID-19 in patients recruited between May-July 2020. Blood samples were analyzed by flow cytometry and ELISA using paired comparison between inclusion (day 0) and 28 days later. The majority of patients were mildly or moderately ill with significantly higher cytokine levels (IL-6 and IL-10) on day 0 as compared with day 28. Platelet activation and granule release were significantly higher on day 0 compared with day 28, as determined by ADP- or thrombin-induced surface CD62P expression, baseline released CD62P, and thrombin-induced platelet-monocyte complex formation. Monocyte activation and procoagulant status at baseline and post activation were heterogeneous but generally lower on day 0 compared with day 28. Baseline and thrombin- or fMLF-induced neutrophil activation and procoagulant status were significantly lower on day 0 compared with day 28. We demonstrate that during the acute phase of COVID-19 compared with the convalescent phase, platelets are more responsive while neutrophils are less responsive. COVID-19 is associated with thromboembolic events where platelet activation and interaction with leukocytes may play an important role

Synaptically-competent neurons derived from canine embryonic stem cells by lineage selection with EGF and noggin

Pluripotent stem cell lines have been generated in several domestic animal species; however, these lines traditionally show poor self-renewal and differentiation. Using canine embryonic stem cell (cESC) lines previously shown to have sufficient self-renewal capacity and potency, we generated and compared canine neural stem cell (cNSC) lines derived by lineage selection with epidermal growth factor (EGF) or Noggin along the neural default differentiation pathway, or by directed differentiation with retinoic acid (RA)-induced floating sphere assay. Lineage selection produced large populations of SOX2+ neural stem/progenitor cell populations and neuronal derivatives while directed differentiation produced few and improper neuronal derivatives. Primary canine neural lines were generated from fetal tissue and used as a positive control for differentiation and electrophysiology. Differentiation of EGF- and Noggin-directed cNSC lines in N2B27 with low-dose growth factors (BDNF/NT-3 or PDGFαα) produced phenotypes equivalent to primary canine neural cells including 3CB2+ radial progenitors, MOSP+ glia restricted precursors, VIM+/GFAP+ astrocytes, and TUBB3+/MAP2+/NFH+/SYN+ neurons. Conversely, induction with RA and neuronal differentiation produced inadequate putative neurons for further study, even though appropriate neuronal gene expression profiles were observed by RT-PCR (including Nestin, TUBB3, PSD95, STX1A, SYNPR, MAP2). Co-culture of cESC-derived neurons with primary canine fetal cells on canine astrocytes was used to test functional maturity of putative neurons. Canine ESC-derived neurons received functional GABAA- and AMPA-receptor mediated synaptic input, but only when co-cultured with primary neurons. This study presents established neural stem/progenitor cell populations and functional neural derivatives in the dog, providing the proof-of-concept required to translate stem cell transplantation strategies into a clinically relevant animal model. © 2011 Wilcox et al

Pneumonia and exposure to household air pollution in children under the age of 5 years in rural Malawi findings from the Cooking And Pneumonia Study

Background Exposure to household air pollution is associated with an increased risk of pneumonia in children in low- and middle-income countries, however exposure-response data are limited and there are uncertainties around the extent to which biomass-fueled cookstoves can reduce these exposures. Research question What is the association between exposure to household air pollution and pneumonia in children under the age of 5 years in rural Malawi and what are the effects of a biomass-fueled cookstove intervention on personal exposure to household air pollution? Study design and methods We measured personal exposure to carbon monoxide (CO) [48 hours of continuous measurement and transcutaneous carboxyhemoglobin (COHb)] 6-monthly in children participating in a cluster-randomised controlled trial of a cleaner-burning biomass-fueled cookstove intervention to prevent pneumonia in children under the age of 5 years in rural Malawi – the Cooking And Pneumonia Study (CAPS). Exposure-response and multi-variable analyses were done. Results We recruited 1805 (928 intervention; 877 control) children (mean age 25.6 months, 50.6% female). We found no evidence of an association between exposure to CO (IRR=1.0 95% CI:0.967-1.014; p=0.53) or COHb (IRR=1.00 95% CI:0.993-1.003; p=0.41)) in children who experienced pneumonia versus those who did not. Median exposure to CO in the intervention and control groups was was 0.34 ppm (IQR 0.15-0.81) and 0.37 ppm (IQR 0.15-0.97), respectively. The group difference in means was 0.46 (95% CI:-0.95-0.012; p=0.06). Interpretation Exposure to CO in our population was low with no association seen between exposure to CO and pneumonia incidence and no effect of the CAPS intervention on these exposures. These findings suggest that CO may not be an appropriate measure of household air pollution exposure in settings like rural Malawi and that there is a need to develop ways to directly measure particulate matter exposures in young children instead