On a Discourse Marker Combination in Spoken American English: Oh Well as a Case Study

This paper investigates a combined discourse marker oh well in spoken American English. The vast majority of research on discourse markers has been conducted on individual items, yet their combinations have received little attention in the literature. In the hope of expanding the knowledge of untapped niche in discourse marker research, the current study aims to examine the functions of a combined discourse marker oh well as a case study. From the analysis, we have found that oh well mostly functions like an amalgamation of two individual discourse markers oh and well, (as a face threat mitigation marker, a discourse initiating marker, a quotative marker, and a topic shift marker), but it sometimes earns extra discourse functions (as a turn exiting marker and a sarcastic speech indexing marker)

Multiplex Real-Time Polymerase Chain Reaction-Based Method for the Rapid Detection of gyrA and parC Mutations in Quinolone-Resistant Escherichia coli and Shigella spp.

AbstractTwo real-time polymerase chain reaction assays were developed to detect mutations in codons 83 and 87 in gyrA and in codons 80 and 91 in parC, the main sites that causes quinolone resistance in pathogenic Escherichia coli and Shigella spp. isolates. These assays can be employed as a useful method for controlling infections caused by quinolone-resistant E coli and Shigella isolates

Effect of Si and SiO 2

Carbon coils could be synthesized using C2H2/H2 as source gases and SF6 as an incorporated additive gas under thermal chemical vapor deposition system. Si substrate, SiO2 thin film deposited Si substrate (SiO2 substrate), and quartz substrate were employed to elucidate the effect of substrate on the formation of carbon coils. The characteristics (formation densities, morphologies, and geometries) of the deposited carbon coils on the substrate were investigated. In case of Si substrate, the microsized carbon coils were dominant on the substrate surface. While, in case of SiO2 substrate, the nanosized carbon coils were prevailing on the substrate surface. The surface morphologies of samples were investigated step by step during the reaction process. The cause for the different geometry formation of carbon coils according to the different substrates was discussed in association with the different thermal expansion coefficient values of Si and SiO2 substrates and the different etched characteristics of Si and SiO2 substrates by SF6 + H2 flow

Simultaneous detection and subtyping of porcine endogenous retroviruses proviral DNA using the dual priming oligonucleotide system

The purpose of this study was to develop a multiplex PCR that can detect porcine endogenous retrovirus (PERV) proviral genes (pol, envA, envB, envC) and porcine mitochondrial DNA, using a dual priming oligonucleotide (DPO) system. The primer specifically detected the PERV proviral genes pol, envA, envB, envC, and porcine mitochondrial DNA only in samples of pig origin. The sensitivity of the primer was demonstrated by simultaneous amplification of all 5 target genes in as little as 10 pg of pig DNA containing PERV proviral genes and mitochondrial DNA. The multiplex PCR, when applied to field samples, simultaneously and successfully amplified PERV proviral genes from liver, blood and hair root samples. Thus, the multiplex PCR developed in the current study using DPO-based primers is a rapid, sensitive and specific assay for the detection and subtyping of PERV proviral genes

Gastropericardial Fistula as a Complication in a Refractory Gastric Ulcer after Esophagogastrostomy with Gastric Pull-Up

A gastropericardial fistula, defined as penetration of a gastric lesion into the pericardium, is a rare occurrence. Such a fistula is usually associated with a huge ulcer in the gastric fundus, an ulcer within a hiatus hernia, a history of esophagogastric surgery, the concurrent use of non-steroidal anti-inflammatory drugs (NSAIDs), or Zollinger-Ellison syndrome. The patient in this case presented with shoulder pain and melena, caused by a gastropericardial fistula that had occurred as a late complication of postoperative esophagogastrostomy and a refractory gastric ulcer. Despite the severity of the condition, the patient showed great improvement after medical treatment and the fistula was cured at the end

Benefits of Recurrent Colonic Stent Insertion in a Patient with Advanced Gastric Cancer with Carcinomatosis Causing Colonic Obstruction

Malignant obstruction develops frequently in advanced gastric cancer. Although it is primarily the gastric outlet that is obstructed, there are occasional reports of colonic obstruction. Treating intestinal obstruction usually requires emergency surgery or stent insertion. There are several kinds of complications with stent insertion, such as bowel perforation, stent migration, bleeding, abdominal pain and reobstruction. Nevertheless, endoscopic stent insertion could be a better treatment than emergency surgery in cases of malignant bowel obstruction in cancer patients with poor performance status. We report a case of advanced gastric cancer with carcinomatosis in which a recurrent colonic stent was inserted at the same site because of cancer growth into the stent. The patient maintained a good condition for chemotherapy, thus improving their chances for survival

Acetylation changes tau interactome to degrade tau in Alzheimer’s disease animal and organoid models

© 2019 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.Alzheimer's disease (AD) is an age-related neurodegenerative disease. The most common pathological hallmarks are amyloid plaques and neurofibrillary tangles in the brain. In the brains of patients with AD, pathological tau is abnormally accumulated causing neuronal loss, synaptic dysfunction, and cognitive decline. We found a histone deacetylase 6 (HDAC6) inhibitor, CKD-504, changed the tau interactome dramatically to degrade pathological tau not only in AD animal model (ADLPAPT) brains containing both amyloid plaques and neurofibrillary tangles but also in AD patient-derived brain organoids. Acetylated tau recruited chaperone proteins such as Hsp40, Hsp70, and Hsp110, and this complex bound to novel tau E3 ligases including UBE2O and RNF14. This complex degraded pathological tau through proteasomal pathway. We also identified the responsible acetylation sites on tau. These dramatic tau-interactome changes may result in tau degradation, leading to the recovery of synaptic pathology and cognitive decline in the ADLPAPT mice11Nsciescopu

Literally does not always mean literally: a corpus-based diachronic study on literally as an intensifier

This paper explores the adverb literallys linguistic state over the past century. Nine-hundred tokens of literally are collected from the Corpus of Historical American English and a social network service, Twitter. Three decades (the 1900s, 1950s, and 2000s) of data are examined as well as the data of 2016. The empirical data is investigated by literallys seven pragmatic functions and syntactic patterns. The results of data analysis are not consistent, but the overall results support the idea that literally is going through grammaticalization, though it is far from a complete internsifier

Modeling narrative structure and dynamics with networks, sentiment analysis, and topic modeling.

Human communication is invariably executed in the form of a narrative, an account of connected events comprising characters, actions, and settings. A coherent and well-structured narrative is therefore essential for effective communication, confusion caused by a haphazard attempt at storytelling being a common experience. This also suggests that a scientific understanding of how a narrative is formed and delivered is key to understanding human communication and dialog. Here we show that the definition of a narrative lends itself naturally to network-based modeling and analysis, and they can be further enriched by incorporating various text analysis methods from computational linguistics. We model the temporally unfolding nature of narrative as a dynamical growing network of nodes and edges representing characters and interactions, which allows us to characterize the story progression using the network growth pattern. We also introduce the concept of an interaction map between characters based on associated sentiments and topics identified from the text that characterize their relationships explicitly. We demonstrate the methods via application to Victor Hugo's Les Misérables. Going beyond simple, aggregate occurrence-based methods for narrative representation and analysis, our proposed methods show promise in uncovering its essential nature of a highly complex, dynamic system that reflects the rich structure of human interaction and communication