Effect of Plant Gowth Regulator on The Gowth of Zygotic Embryos in Three Types of Oil Palm Fruit (Elaeis guineensis Jacq.) in Tissue Culture

Surahman H, Sulaksono G, Sembiring Z, Kurnianingsih A, Priadi DP, Asmono D. 2020. Effect of plant gowth regulator on the gowth of zygotic embryos in three types of oil palm fruit (Elaeis guineensis Jacq.) in tissue culture. Jurnal Lahan Suboptimal : Journal of Suboptimal Lands 9(2): 149-159.Embryo culture is a widely used and effective way of overcoming the dormancy nature of seeds in oil palm.  At the germination stage, the influence of gowth regulators play an important role although in relatively lower concentrations in the germination media. This aim research determined the effect of adding various concentrations of gowth regulator on the three types of oil palm fruit based on the thickness of the shell. This research carried out at the Tissue Culture Laboratory of PT. Sampoerna Ago Tbk. from September 2018 to January 2019. The gowth regulator substances used in this study were NAA, BAP and GA3. The composition of the gowth regulators used were H1 = 0.05 mg/L NAA + 0.1 mg/L BAP + 0.1 mg/L GA3, H2 = 0.05 mg/L NAA + 0.3 mg/L BAP + 0.1 mg/L GA3, H3 = 0.05 mg/L NAA + 0.5 mg/L BAP + 0.1 mg/L GA3 and H4 = No gowth regulator. While the types of oil palm fruit used were C1 = Dura, C2 = Tenera and C3 = Pisifera. This research used a factorial complete randomized design with three replications. The results showed that in general the use of gowth regulator substances H1 = 0.05 mg/L NAA + 0.1 mg/L BAP + 0.1 mg/L GA3 and the type of dura fruit showed positive results based on viability, scoring, survival and height of plantlets when compared with other treatments

Pengelolaan Kebun Induk Kelapa Sawit (Elaeis guinensis Jacq.) pada Lahan Suboptimal di PT Binasawit Makmur

Sirait et al, 2019. Maintaining of Seed Garden for Suboptimal Area in PT Binasawit Makmur. pp. 360-365.   Continual improvement of seed production is very important to fulfill of oil palm sprouts demand. To fulfill that demand, suboptimal land factor that so far being limiting factors should be minimized so that’s not to inhibit the production of oil palm seeds. This reasearch aim was maintaining of seed garden for suboptimal area  in PT Binasawit Makmur.  PT. Binasawit Makmur, Sampoerna Agro Tbk. is one of the seed producers in Indonesia which has the selected Dura mains planted on suboptimal land were located on Mesuji Districts, Ogan Komering Ilir Regency, South Sumatera Province. Maintaining of Dura palm strategy is needed by stricty Quality Control of processes and environmental conditions in the field to produce quality seeds. Optimalization of seed production on sub-optimal land are by adding organic matter to the soil and applying irrigation systems to the selected elite Dura palms. The addition of organic matter and applying of irrigation systems can made continuity of seed production with optimal quality and quantity so that the demand for oil palm sprouts for custumer satisfaction

Strategi Program Pemuliaan Tanaman Kelapa Sawit (Elaeis guinensis Jacq.) pada Lahan Suboptimal di PT Binasawit Makmur

Pratama et al, 2018. Oil Palm Breeding Program Strategy for Suboptimal Area in PT Binasawit Makmur  . JLSO 7(1):26-36. Suboptimal areas have great potential to be used an alternative land for cultivation of oil palm.We needed superior oil palm material to be able to grow well on that land. PT. Binasawit Makmur (BSM) through the Breeding Unit has developed a strategy regarding the assembly of superior material oil palm drought resistant. Therefore, this research aimed to develop  a strategy regarding breeding program in suboptimal lands of PT Binasawit Makmur.  The strategies used were  introducing, evaluating, and selecting genetic material based on productivity components (total and weight of fresh fruit bunches/ha, extraction rate) and high increment.  In addition, the efforts have been made to obtain drought tolerant material are conducting a progeny trial (DXP Trial) on mature palm to obtain drought tolerant in the dried season. Based on the results of the progeny trial, both of parents were traced to a crossing program. Currently, the seeds of the crossing have been obtained to confirm the results of the research on the nursery scale. In the nursery trial, research will be conducted to measure the proline content, stomata and chlorophyll content. Selection in the mature palm DXP progeny trial was followed by application a molecular tool such as SSR and SNP. Through this approach, a superior genetic material in terms of productivity and also drought tolerance can be developed and therefore can be used for recombination and release of new varieties

Association Mapping between Candidate Gene SNP and Production and Oil Quality Traits in Interspecific Oil Palm Hybrids

Oil palm production is gaining importance in Central and South America. However, the main species Elaeis guineensis (Eg) is suffering severely from bud rod disease, restricting the potential cultivation areas. Therefore, breeding companies have started to work with interspecific Elaeis oleifera x Eg (Eo x Eg) hybrids which are tolerant to this disease. We performed association studies between candidate gene (CG) single nucleotide polymorphisms (SNP) and six production and 19 oil quality traits in 198 accessions of interspecific oil palm hybrids from five different origins. For this purpose, barcoded amplicons of initially 167 CG were produced from each genotype and sequenced with Ion Torrent. After sequence cleaning 115 SNP remained targeting 62 CG. The influence of the origins on the different traits was analyzed and a genetic diversity study was performed. Two generalized linear models (GLM) with principle component analysis (PCA) or structure (Q) matrixes as covariates and two mixed linear models (MLM) which included in addition a Kinship (K) matrix were applied for association mapping using GAPIT. False discovery rate (FDR) multiple testing corrections were applied in order to avoid Type I errors. However, with FDR adjusted p values no significant associations between SNP and traits were detected. If using unadjusted p values below 0.05, seven of the studied CG showed potential associations with production traits, while 23 CG may influence different quality traits. Under these conditions the current approach and the detected candidate genes could be exploited for selecting genotypes with superior CG alleles in Marker Assisted Selection systems