Stability of Hematological Parameters in Woodland Caribou (Rangifer tarandus caribou) Blood Stored at 4°C

Eighteen free-ranging female woodland caribou were captured in northern Alberta in January and February 1993. Blood was collected into ethylenediaminetetraacetate (EDTA) tubes which were packaged in coolers containing ice packs, and transported to the laboratory where they arrived within 48 hrs of collection. Complete blood counts (CBC) were performed on five consecutive days to assess the stability of hematological parameters. Average values of hematocrit (HCT), mean cell hemoglobin (MCH), mean cell volume (MCV), red cell distribution width (P-J3W), white blood cell count (WBC), and red blood cell count (RJ3C) remained stable with no statistically significant changes occurring during 5 days of post-collection storage at 4°C. Mean PvBC values exhibited significant differences (p<0.05) between geographic locations. Mean platelet volume (MPV) increased significantly (p<0.001) with storage time, while platelet (PLT) values decreased (p<0.001) over time and were significantly different (p<0.01) between locations. For optimal hematological results, it is recommended that sample analysis be performed within 24 hours of blood collection; however, if caribou blood samples are properly stored at 4°C, useful information may be obtained from stable parameters up to 5 days following collection

Limiting nutrients for bean production on contrasting soil types of Lake Victoria Crescent of Uganda

Common bean (Phaseolus vulgaris L.) is one of the most important grain legumes in East Africa, but its yield has remained below the genetic potential. Declining soil fertility is among the primary constraints to bean production in most East African bean producing regions. Often existing recommendations are generic and inept to guide farm level decision making on nutrient replenishment. A greenhouse nutrient omission study was conducted to determine the limiting nutrients in three soils of Masaka District, commonly cropped to beans: “Liddugavu” a Phaeozem, “Limyufumyufu” a Cambisol and “Luyinjayinga” an Umbrisol soil. Nine treatments; (i) complete nutrient treatment, (ii) N omitted, (iii) P omitted, (iv) K omitted, (v) Mg omitted, (vi) S omitted, (vii) Ca omitted, (viii) Micronutrients omitted and (ix) control without nutrients. Each treatment was randomly assigned to the three soils and replicated three times using a completely randomised design. Nitrogen, phosphorus and potassium were limiting nutrients for bean production in Umbrisol (Luyinjayinja) while in Cambisol (‘Limyufumyufu), common bean production was most limited by soil acidity. The performance varied with soil types, with beans grown on the Phaeozem registering greater leaf number and growth, confirming both scientist’s and local farmer’s knowledge that this soil has greater potential than the other two soils

Limiting nutrients for bean production on contrasting soil types of Lake Victoria Crescent of Uganda

Common bean (Phaseolus vulgaris L.) is one of the most important grain legumes in East Africa, but its yield has remained below the genetic potential. Declining soil fertility is among the primary constraints to bean production in most East African bean producing regions. Often existing recommendations are generic and inept to guide farm level decision making on nutrient replenishment. A greenhouse nutrient omission study was conducted to determine the limiting nutrients in three soils of Masaka District, commonly cropped to beans: \u201cLiddugavu\u201d a Phaeozem, \u201cLimyufumyufu\u201d a Cambisol and \u201cLuyinjayinga\u201d an Umbrisol soil. Nine treatments; (i) complete nutrient treatment, (ii) N omitted, (iii) P omitted, (iv) K omitted, (v) Mg omitted, (vi) S omitted, (vii) Ca omitted, (viii) Micronutrients omitted and (ix) control without nutrients. Each treatment was randomly assigned to the three soils and replicated three times using a completely randomised design. Nitrogen, phosphorus and potassium were limiting nutrients for bean production in Umbrisol (Luyinjayinja) while in Cambisol (\u2018Limyufumyufu), common bean production was most limited by soil acidity. The performance varied with soil types, with beans grown on the Phaeozem registering greater leaf number and growth, confirming both scientist\u2019s and local farmer\u2019s knowledge that this soil has greater potential than the other two soils.Le haricot commun (Phaseolus vulgaris L.) est un des l\ue9gumes \ue0 grains les plus importants en Afrique de l\u2019Est, mais son rendement reste toujours en dessous de son potentiel g\ue9n\ue9tique. La baisse de la fertilit\ue9 du sol est parmi les contraintes primaires \ue0 la production du haricot dans la plupart des r\ue9gions productrices de l\u2019Afrique de l\u2019Est. Le plus souvent, les recommandations sont g\ue9n\ue9riques et inad\ue9quates pour guider la prise de d\ue9cision au niveau champ sur le r\ue9approvisionnement en nutriment. Une \ue9tude sous serre sur l\u2019omission de nutriment a \ue9t\ue9 conduite pour d\ue9terminer les nutriments limitants dans les trois sols du district de Masaka, commun\ue9ment utilis\ue9s pour produire du haricot\ua0: \u201cLiddugavu\u201d un sol du Phaeozem, \u201cLimyufumyufu\u201d un sol du Cambisol et \u201cLuyinjayinga\u201d un sol du Umbrisol. Neuf traitements, (i) traitement complet de nutriments, (ii) N omis, (iii) P omis, (iv) K omis, (v) Mg omis, (iv) S omis, (vii) Ca omis, (viii) micronutriments omis et (ix) control sans nutriments. Chacun des traitements a \ue9t\ue9 al\ue9atoirement distribu\ue9 aux trois types de sols et r\ue9pliqu\ue9 trois fois dans un dispositif compl\ue8tement al\ue9atoire. Azote, phosphore, et potassium ont \ue9t\ue9 les nutriments limitants pour la production du haricot dans Umbrisol (Luyinjayinja) tandis que dans Cambisol (\u2018Limyufumyufu), la production du haricoct commun a \ue9t\ue9 limit\ue9e par l\u2019acidit\ue9 du sol. Les performances varient en fonction des types de sols, avec le haricot produit sur le Phaeozem comptant plus de feuilles et de croissance, confirmant \ue0 la fois les connaissances des scientifiques et des populations locales qui stipulent que le sol a un potentiel plus \ue9lev\ue9 que les deux autres sols

Immediate effect of the COVID-19 pandemic on patient health, health-care use, and behaviours : results from an international survey of people with rheumatic diseases

Funding Information: We would like to thank all the clinicians, health-care providers, and patient organisations who helped to develop and disseminate this survey. A full list of all the contributors can be found in the appendix (pp 44?45). Preliminary results were presented at the American College of Rheumatology 2020 conference. The views expressed here are those of the authors and participating members of the COVID-19 Global Rheumatology Alliance and do not necessarily represent the views of the American College of Rheumatology, the European League Against Rheumatism, the UK National Health Service, the National Institute for Health Research, the UK Department of Health, or any other organisation. Funding Information: JSH reports grants from Childhood Arthritis and Rheumatology Research Alliance and Rheumatology Research Alliance; and personal fees from Novartis, Pfizer, and Biogen, outside of the submitted work. JWL reports grants from Pfizer, outside of the submitted work. JAS reports grants and personal fees from Bristol-Myers Squibb; and personal fees from Gilead, Inova Diagnostics, Optum, and Pfizer, outside of the submitted work. CH reports personal fees from AstraZeneca and Aurinia Pharmaceuticals, outside of the submitted work. MJL reports grants from American College of Rheumatology during the conduct of the study and consulting fees from AbbVie, Amgen, Actelion, Boehringer Ingelheim, BMS, Celgene, Gilead, Johnson & Johnson, Mallinckrodt, Novartis, Pfizer, Roche, Sandoz, Sanofi, Sobi, and UCB, outside of the submitted work. SES is supported by the Vasculitis Clinical Research Consortium and Vasculitis Foundation outside of the submitted work. KLD reports grants from Novartis, Sobi, National Institutes of Health, and Horizon Bio, outside of the submitted work. EFM reports that the Liga Portuguesa Contra as Doenças Reumaticas received support for specific activities: grants from Abbvie, Novartis, Janssen-Cilag, Lilly Portugal, Sanofi, Grünenthal SA, MSD, Celgene, Medac, Pharmakern, GAfPA, AMGEN, A Menarini Portugal; grants and non-financial support from Pfizer; and non-financial support from Grünenthal GmbH and Tilray, outside of the submitted work. DPR is the volunteer Vice President of the Canadian Arthritis Patient Alliance, which is primarily supported by independent grants from pharmaceutical companies. DPR reports consulting fees from NovoNordisk Canada and speaking fees and an honoraria from Eli Lilly Canada, outside of the submitted work. DPR also lives with rheumatoid arthritis. SB reports personal fees from Novartis, AbbVie, Pfizer, and Horizon Pharma, outside of the submitted work. RG reports personal fees from AbbVie New Zealand, Cornerstones, Janssen New Zealand; and personal fees and non-financial support from Pfizer New Zealand, (all <$10 000) outside of the submitted work. PMM reports personal fees from Abbvie, Eli Lilly, Janssen, Novartis, Pfizer, and UCB; and grants and personal fees from Orphazyme, outside of the submitted work. PCR reports personal fees from Abbvie, Gilead, Lilly, and Roche; grants and personal fees from Novartis, UCB Pharma, Janssen, and Pfizer; and non-financial support from BMS, outside of the submitted work. PS reports honoraria from being a social media editor for @ACR_Journals, outside of the submitted work. ZSW reports grants from National Institutes of Health, BMS, and Sanofi; and personal fees from Viela Bio and MedPace, outside of the submitted work. JY reports personal fees from Pfizer and Eli Lilly, and grants and personal fees from Astra Zeneca, outside of the submitted work. ES is a Board Member of the Canadian Arthritis Patient Alliance, which is a patient-run, volunteer-based organisation whose activities are primarily supported by independent grants from pharmaceutical companies. All other authors declare no competing interests. Publisher Copyright: © 2021 Elsevier LtdBackground: The impact and consequences of the COVID-19 pandemic on people with rheumatic disease are unclear. We developed the COVID-19 Global Rheumatology Alliance Patient Experience Survey to assess the effects of the COVID-19 pandemic on people with rheumatic disease worldwide. Methods: Survey questions were developed by key stakeholder groups and disseminated worldwide through social media, websites, and patient support organisations. Questions included demographics, rheumatic disease diagnosis, COVID-19 diagnosis, adoption of protective behaviours to mitigate COVID-19 exposure, medication access and changes, health-care access and communication with rheumatologists, and changes in employment or schooling. Adults age 18 years and older with inflammatory or autoimmune rheumatic diseases were eligible for inclusion. We included participants with and without a COVID-19 diagnosis. We excluded participants reporting only non-inflammatory rheumatic diseases such as fibromyalgia or osteoarthritis. Findings: 12 117 responses to the survey were received between April 3 and May 8, 2020, and of these, 10 407 respondents had included appropriate age data. We included complete responses from 9300 adults with rheumatic disease (mean age 46·1 years; 8375 [90·1%] women, 893 [9·6%] men, and 32 [0·3%] participants who identified as non-binary). 6273 (67·5%) of respondents identified as White, 1565 (16·8%) as Latin American, 198 (2·1%) as Black, 190 (2·0%) as Asian, and 42 (0·5%) as Native American or Aboriginal or First Nation. The most common rheumatic disease diagnoses included rheumatoid arthritis (3636 [39·1%] of 9300), systemic lupus erythematosus (2882 [31·0%]), and Sjögren's syndrome (1290 [13·9%]). Most respondents (6921 [82·0%] of 8441) continued their antirheumatic medications as prescribed. Almost all (9266 [99·7%] of 9297) respondents adopted protective behaviours to limit SARS-CoV-2 exposure. A change in employment status occurred in 2524 (27·1%) of 9300) of respondents, with a 13·6% decrease in the number in full-time employment (from 4066 to 3514). Interpretation: People with rheumatic disease maintained therapy and followed public health advice to mitigate the risks of COVID-19. Substantial employment status changes occurred, with potential implications for health-care access, medication affordability, mental health, and rheumatic disease activity. Funding: American College of Rheumatology.publishersversionPeer reviewe

Effect of redox conditions on phosphate exchangeability and iron forms in a soil amended with ferrous iron

Application of iron (Fe) -rich amendments to soils has been proposed as a means of decreasing phosphorus (P) losses from soils. However, anoxic conditions following soil saturation are known to increase Fe and P solubility in soils, thus cancelling out the potential benefits. Our aim was to evaluate the effects of continuous oxic, continuous anoxic and alternating anoxic/oxic conditions on P exchangeability and Fe forms in soil amended with Ca(OH)(2) and FeSO(4). We incubated amended and unamended soils under these conditions for 8 weeks and measured Fe forms and P exchangeability. Under oxic conditions, addition of Ca(OH)(2) and FeSO(4) resulted in a strong decrease in P exchangeability and an increase in oxalate-extractable Fe. Mossbauer analyses suggested that an unidentified Fe oxide (D1oxide) with a strong sorbing capacity for P was precipitated. Under continuously anoxic conditions, P exchangeability and oxalate-extractable Fe increased with or without the amendments. Mossbauer analyses suggested that there was a partial dissolution of the D1oxide phase, precipitation of another unidentified Fe oxide (S3) and a reduction of structural Fe(3+) in phyllosilicate, thereby increasing soil negative charge. These transformations resulted in a strong increase in rapidly exchangeable P. Alternating anoxic and oxic periods induced the dissolution and precipitation of iron oxides and the increase and decrease in P exchangeability. Implications of the results for limiting P losses from grassland soils are discussed

Limiting nutrients for bean production on contrasting soil types of Lake Victoria Crescent of Uganda

Common bean (Phaseolus vulgaris L.) is one of the most important grain legumes in East Africa, but its yield has remained below the genetic potential. Declining soil fertility is among the primary constraints to bean production in most East African bean producing regions. Often existing recommendations are generic and inept to guide farm level decision making on nutrient replenishment. A greenhouse nutrient omission study was conducted to determine the limiting nutrients in three soils of Masaka District, commonly cropped to beans: “Liddugavu” a Phaeozem, “Limyufumyufu” a Cambisol and “Luyinjayinga” an Umbrisol soil. Nine treatments; (i) complete nutrient treatment, (ii) N omitted, (iii) P omitted, (iv) K omitted, (v) Mg omitted, (vi) S omitted, (vii) Ca omitted, (viii) Micronutrients omitted and (ix) control without nutrients. Each treatment was randomly assigned to the three soils and replicated three times using a completely randomised design. Nitrogen, phosphorus and potassium were limiting nutrients for bean production in Umbrisol (Luyinjayinja) while in Cambisol (‘Limyufumyufu), common bean production was most limited by soil acidity. The performance varied with soil types, with beans grown on the Phaeozem registering greater leaf number and growth, confirming both scientist’s and local farmer’s knowledge that this soil has greater potential than the other two soils.This article is published as Kyomuhendo, P., M.M. Tenywa, O. Semalulu, A. Lenssen, R. Yost, S. Kyebegola, L. Goettsch, R. Mazur. 2018. Limiting nutrients for bean production on three contrasting soils. African Crop Science Journal. 26:543-554. doi: 10.4314/acsj.v26i4.8.</p