12 research outputs found
2021 The SELA Agenda
The SELA Agenda is a collectively and inclusively drafted report that addresses the impact of COVID-19 in the Southeast Los Angeles (SELA) region by highlighting investment opportunities in eight policy areas: education, environmental justice, economic recovery, healthcare, housing, nonprofit safety net, civic engagement and regional advocacy. The goal is to lay out a COVID-19 recovery plan that prioritizes the SELA region and ensures the region's recovery and future prosperity by bringing SELA's needs to the attention of elected officials, philanthropy, business sector, and community stakeholders.
An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for <em>in vivo</em> Tumor Imaging and Monitoring of Tumor Immune Responses
Analysis of the LAT adapter role in the development and in the function of T lymphocytes using knock-in mice
AIX-MARSEILLE2-BU Sci.Luminy (130552106) / SudocSudocFranceF
Differential Requirement for the SAP-Fyn Interaction during NK T Cell Development and Function
Improving T Cell Expansion with a Soft Touch
Protein-coated microbeads
provide a consistent approach for activating
and expanding populations of T cells for immunotherapy but do not
fully capture the properties of antigen presenting cells. In this
report, we enhance T cell expansion by replacing the conventional,
rigid bead with a mechanically soft elastomer. Polydimethylsiloxane
(PDMS) was prepared in a microbead format and modified with activating
antibodies to CD3 and CD28. A total of three different formulations
of PDMS provided an extended proliferative phase in both CD4<sup>+</sup>-only and mixed CD4<sup>+</sup>–CD8<sup>+</sup> T cell preparations.
CD8<sup>+</sup> T cells retained cytotoxic function, as measured by
a set of biomarkers (perforin production, LAMP2 mobilization, and
IFN-γ secretion) and an in vivo assay of targeted cell killing.
Notably, PDMS beads presented a nanoscale polymer structure and higher
rigidity than that associated with conventional bulk material. These
data suggest T cells respond to this higher rigidity, indicating an
unexpected effect of curing conditions. Together, these studies demonstrate
that adopting mechanobiology ideas into the bead platform can provide
new tools for T cell based immunotherapy