50 research outputs found
Transition from self-supported to supported living: Older people's experiences
To become dependent on professional support to accomplish the daily activities of life can be considered a turning point, involving a range of challenging changes in life. The purpose of the study was to describe the experiences of older home-dwelling individuals in transition from self-supported to supported living from a lifeworld perspective. Five women and five men were interviewed, and a descriptive phenomenological design was used. The findings showed that an attitude of acceptance was an essential characteristic for this group. An attitude of acceptance comprised: flexibility and tolerance, recognition and hopes, and valuation of self and situation. Finding themselves in a situation they had to submit to, they took an attitude of acceptance. An attitude of acceptance implied acknowledgement of the situation as well as positivity and desires to manage. This attitude may represent a significant potential for improvement. Awareness of this is crucial to support older individuals in a healthy way through the transition process. An attitude of acceptance, however, also implied an acceptance of discontinuity in their lives, renunciations, and denigration of own needs. But this aspect of the acceptance was trivialized by the participants and not equally obvious. Insight into this complexity is vital to avoid ignorance of older individualsâ vulnerability in the transition process
ActinoBase: tools and protocols for researchers working on Streptomyces and other filamentous actinobacteria
Selection of Streptomyces against soil borne fungal pathogens by a standardized dual culture assay and evaluation of their effects on seed germination and plant growth
Recommended from our members
Minimum Information about a Biosynthetic Gene cluster
A wide variety of enzymatic pathways that produce specialized metabolites in bacteria, fungi and plants are known to be encoded in biosynthetic gene clusters. Information about these clusters, pathways and metabolites is currently dispersed throughout the literature, making it difficult to exploit. To facilitate consistent and systematic deposition and retrieval of data on biosynthetic gene clusters, we propose the Minimum Information about a Biosynthetic Gene cluster (MIBiG) data standard.Chemistry and Chemical Biolog
Disulphide Bridge Formation of Proinsulin Fusion Proteins during Secretion in Streptomyces
Biosynthesis of Antimycins with a Reconstituted 3âFormamidosalicylate Pharmacophore in <i>Escherichia coli</i>
Antimycins
are a family of natural products generated from a hybrid
nonribosomal peptide synthetase (NRPS)-polyketide synthase (PKS) assembly
line. Although they possess an array of useful biological activities,
their structural complexity makes chemical synthesis challenging,
and their biosynthesis has thus far been dependent on slow-growing
source organisms. Here, we reconstituted the biosynthesis of antimycins
in <i>Escherichia coli</i>, a versatile host that is robust
and easy to manipulate genetically. Along with <i>Streptomyces</i> genetic studies, the heterologous expression of different combinations
of <i>ant</i> genes enabled us to systematically confirm
the functions of the modification enzymes, AntHIJKL and AntO, in the
biosynthesis of the 3-formamidosalicylate pharmacophore of antimycins.
Our <i>E. coli</i>-based antimycin production system can
not only be used to engineer the increased production of these bioactive
compounds, but it also paves the way for the facile generation of
novel and diverse antimycin analogues through combinatorial biosynthesis