5,294 research outputs found
Hydrodynamic lift on bound vesicles
Bound vesicles subject to lateral forces such as arising from shear flow are
investigated theoretically by combining a lubrication analysis of the bound
part with a scaling approach to the global motion. A minor inclination of the
bound part leads to significant lift due to the additive effects of lateral and
tank-treading motions. With increasing shear rate, the vesicle unbinds from the
substrate at a critical value. Estimates are in agreement with recent
experimental data.Comment: 9 pages, one figur
Bilayer Membrane in Confined Geometry: Interlayer Slide and Steric Repulsion
We derived free energy functional of a bilayer lipid membrane from the first
principles of elasticity theory. The model explicitly includes
position-dependent mutual slide of monolayers and bending deformation. Our free
energy functional of liquid-crystalline membrane allows for incompressibility
of the membrane and vanishing of the in-plane shear modulus and obeys
reflectional and rotational symmetries of the flat bilayer. Interlayer slide at
the mid-plane of the membrane results in local difference of surface densities
of the monolayers. The slide amplitude directly enters free energy via the
strain tensor. For small bending deformations the ratio between bending modulus
and area compression coefficient, Kb/KA, is proportional to the square of
monolayer thickness, h. Using the functional we performed self-consistent
calculation of steric potential acting on bilayer between parallel confining
walls separated by distance 2d. We found that temperature-dependent curvature
at the minimum of confining potential is enhanced four times for a bilayer with
slide as compared with a unit bilayer. We also calculate viscous modes of
bilayer membrane between confining walls. Pure bending of the membrane is
investigated, which is decoupled from area dilation at small amplitudes. Three
sources of viscous dissipation are considered: water and membrane viscosities
and interlayer drag. Dispersion has two branches. Confinement between the walls
modifies the bending mode with respect to membrane in bulk solution.
Simultaneously, inter-layer slipping mode, damped by viscous drag, remains
unchanged by confinement.Comment: 23 pages,3 figures, pd
Generation of specific antibodies against the rap1A, rap1B and rap2 small GTP-binding proteins. Analysis of rap and ras proteins in membranes from mammalian cells
Specific antibodies against rap1A and rap1B small GTP-binding proteins were generated by immunization of rabbits with peptides derived from the C-terminus of the processed proteins. Immunoblot analysis of membranes from several mammalian cell lines and human thrombocytes with affinity-purified antibodies against rap1A or rap1B demonstrated the presence of multiple immunoreactive proteins in the 22-23 kDa range, although at strongly varying levels. Whereas both proteins were present in substantial amounts in membranes from myelocytic HL-60, K-562 and HEL cells, they were hardly detectable in membranes from lymphoma U-937 and S49.1 cyc- cells. Membranes from human thrombocytes and 3T3-Swiss Albino fibroblasts showed strong rap1B immunoreactivity, whereas rap1A protein was present in much lower amounts. In the cytosol of HL-60 cells, only small amounts of rap1A and rap1B proteins were detected, unless the cells were treated with lovastatin, an inhibitor of hydroxymethylglutaryl-coenzyme A reductase, suggesting that both proteins are isoprenylated. By comparison with recombinant proteins, the ratio of rap1A/ras proteins in membranes from HL-60 cells was estimated to be about 4:1. An antiserum directed against the C-terminus of rap2 reacted strongly with recombinant rap2, but not with membranes from tested mammalian cells. In conclusion, rap1A and rap1B proteins are distributed differentially among membranes from various mammalian cell types and are isoprenylated in HL-60 cells
Can the Tajmar effect be explained using a modification of inertia?
The Tajmar effect is an unexplained acceleration observed by accelerometers
and laser gyroscopes close to rotating supercooled rings. The observed ratio
between the gyroscope and ring accelerations was 3+/-1.2x10^-8. Here, a new
model for inertia which has been tested quite successfully on the Pioneer and
flyby anomalies is applied to this problem. The model assumes that the inertia
of the gyroscope is caused by Unruh radiation that appears as the ring and the
fixed stars accelerate relative to it, and that this radiation is subject to a
Hubble-scale Casimir effect. The model predicts that the sudden acceleration of
the nearby ring causes a slight increase in the inertial mass of the gyroscope,
and, to conserve momentum in the reference frame of the spinning Earth, the
gyroscope rotates clockwise with an acceleration ratio of 1.8+/-0.25x10^-8 in
agreement with the observed ratio. However, this model does not explain the
parity violation seen in some of the gyroscope data. To test these ideas the
Tajmar experiment (setup B) could be exactly reproduced in the southern
hemisphere, since the model predicts that the anomalous acceleration should
then be anticlockwise.Comment: 9 pages, 1 figure. Accepted by EPL on the 4th December, 200
Efficiency at maximum power: An analytically solvable model for stochastic heat engines
We study a class of cyclic Brownian heat engines in the framework of
finite-time thermodynamics. For infinitely long cycle times, the engine works
at the Carnot efficiency limit producing, however, zero power. For the
efficiency at maximum power, we find a universal expression, different from the
endoreversible Curzon-Ahlborn efficiency. Our results are illustrated with a
simple one-dimensional engine working in and with a time-dependent harmonic
potential.Comment: 6 pages, 3 figure
Tidal controls on trace gas dynamics in a seagrass meadow of the Ria Formosa lagoon (southern Portugal)
Coastal zones are important source regions for a variety of trace gases, including halocarbons and sulfur-bearing species. While salt marshes, macroalgae and phyto-plankton communities have been intensively studied, little is known about trace gas fluxes in seagrass meadows. Here we report results of a newly developed dynamic flux chamber system that can be deployed in intertidal areas over full tidal cycles allowing for highly time-resolved measurements. The fluxes of CO2, methane (CH4) and a range of volatile organic compounds (VOCs) showed a complex dynamic mediated by tide and light. In contrast to most previous studies, our data indicate significantly enhanced fluxes during tidal immersion relative to periods of air exposure. Short emission peaks occurred with onset of the feeder current at the sampling site. We suggest an overall strong effect of advective transport processes to explain the elevated fluxes during tidal immersion. Many emission estimates from tidally influenced coastal areas still rely on measurements carried out during low tide only. Hence, our results may have significant implications for budgeting trace gases in coastal areas. This dynamic flux chamber system provides intensive time series data of community respiration (at night) and net community production (during the day) of shallow coastal systems.German Federal Ministry of Education and Research (BMBF) [03F0611E, 03F0662E]; EU FP7 ASSEMBLE research infrastructure initiative
Influence of shear flow on vesicles near a wall: a numerical study
We describe the dynamics of three-dimensional fluid vesicles in steady shear
flow in the vicinity of a wall. This is analyzed numerically at low Reynolds
numbers using a boundary element method. The area-incompressible vesicle
exhibits bending elasticity. Forces due to adhesion or gravity oppose the
hydrodynamic lift force driving the vesicle away from a wall. We investigate
three cases. First, a neutrally buoyant vesicle is placed in the vicinity of a
wall which acts only as a geometrical constraint. We find that the lift
velocity is linearly proportional to shear rate and decreases with increasing
distance between the vesicle and the wall. Second, with a vesicle filled with a
denser fluid, we find a stationary hovering state. We present an estimate of
the viscous lift force which seems to agree with recent experiments of Lorz et
al. [Europhys. Lett., vol. 51, 468 (2000)]. Third, if the wall exerts an
additional adhesive force, we investigate the dynamical unbinding transition
which occurs at an adhesion strength linearly proportional to the shear rate.Comment: 17 pages (incl. 10 figures), RevTeX (figures in PostScript
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Roles of inventory and reserve capacity in mitigating supply chain disruption risk
This research focuses on managing disruption risk in supply chains using inventory and reserve capacity under stochastic demand. While inventory can be considered as a speculative risk mitigation lever, reserve capacity can be used in a reactive fashion when a disruption occurs. We determine optimal inventory levels and reserve capacity production rates for a firm that is exposed to supply chain disruption risk. We fully characterize four main risk mitigation strategies: inventory strategy, reserve capacity strategy, mixed strategy
and passive acceptance. We illustrate how the optimal risk mitigation strategy depends on product characteristics (functional versus innovative) and supply chain characteristics (agile versus efficient). This work is inspired from a risk management problem of a leading pharmaceutical company
Periodically driven stochastic un- and refolding transitions of biopolymers
Mechanical single molecule experiments probe the energy profile of
biomolecules. We show that in the case of a profile with two minima (like
folded/unfolded) periodic driving leads to a stochastic resonance-like
phenomenon. We demonstrate that the analysis of such data can be used to
extract four basic parameters of such a transition and discuss the statistical
requirements of the data acquisition. As advantages of the proposed scheme, a
polymeric linker is explicitly included and thermal fluctuations within each
well need not to be resolved.Comment: 7 pages, 5 figures, submitted to EP
Elastic deformation of a fluid membrane upon colloid binding
When a colloidal particle adheres to a fluid membrane, it induces elastic
deformations in the membrane which oppose its own binding. The structural and
energetic aspects of this balance are theoretically studied within the
framework of a Helfrich Hamiltonian. Based on the full nonlinear shape
equations for the membrane profile, a line of continuous binding transitions
and a second line of discontinuous envelopment transitions are found, which
meet at an unusual triple point. The regime of low tension is studied
analytically using a small gradient expansion, while in the limit of large
tension scaling arguments are derived which quantify the asymptotic behavior of
phase boundary, degree of wrapping, and energy barrier. The maturation of
animal viruses by budding is discussed as a biological example of such
colloid-membrane interaction events.Comment: 14 pages, 9 figures, REVTeX style, follow-up on cond-mat/021242
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